BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg350042
Length=1587
Score E
Sequences producing significant alignments: (Bits) Value
AT5G07830.1 | Symbols: AtGUS2, GUS2 | glucuronidase 2 | chr5:25... 2401 0.0
AT5G61250.2 | Symbols: AtGUS1, GUS1 | glucuronidase 1 | chr5:24... 898 0.0
> AT5G07830.1 | Symbols: AtGUS2, GUS2 | glucuronidase 2 | chr5:2503323-2506688
FORWARD LENGTH=1954
Length=1954
Score = 2401 bits (1300), Expect = 0.0
Identities = 1498/1590 (94%), Gaps = 27/1590 (2%)
Strand=Plus/Plus
Query 1 ATGGCTCAAGAAACGAAACGTGCATCGATCGTTGTGGAAGGAGCCCGTCGAGTCTGTGAG 60
||||||||||||| ||||||||||||||||||| | ||||||| || ||||| ||||||
Sbjct 268 ATGGCTCAAGAAATGAAACGTGCATCGATCGTTATCCAAGGAGCTCGACGAGTTTGTGAG 327
Query 61 ACCGACGAGAACTTTGTCTGTGCCACATTGGACTGGTGGCCTCATGACAAGTGCAACTAT 120
||||| ||||||||||||||||| ||||||||||||||||||||||||||||||||||||
Sbjct 328 ACCGATGAGAACTTTGTCTGTGCGACATTGGACTGGTGGCCTCATGACAAGTGCAACTAT 387
Query 121 GACCAATGTCCTTGGGGTTACTCTTCAGTTATCAATATGGATTTAACTCGTCCCCTTCTT 180
||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
Sbjct 388 GACCAATGTCCTTGGGGTTACTCTTCAGTTATCAATATGGATTTAACGCGTCCCCTTCTT 447
Query 181 ACTAAAGCTATTCAAGCTTTCAAGCCATTGAGGATAAGAATTGGTGGTTC-CTTGCAAGA 239
|||||||||||| ||||||||||||| ||||||||||||||||||||||| | |||||||
Sbjct 448 ACTAAAGCTATTAAAGCTTTCAAGCCGTTGAGGATAAGAATTGGTGGTTCGC-TGCAAGA 506
Query 240 TCAAGTAACGTACGACGTAGGAAATCTCAAAACTCCTTGCCGTCCATTCCAAAAAATGAA 299
|||||| | ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 507 TCAAGTTATTTACGACGTAGGAAATCTCAAAACTCCTTGCCGTCCATTCCAAAAAATGAA 566
Query 300 CAGCGGTTTGTTTGGATTCTCTAAAGGATGCTTGCACATGAAGA-GATGGGACGAGCTCA 358
|||||||||||||||||||||||||||||||||||||||||| | |||||||||||||||
Sbjct 567 CAGCGGTTTGTTTGGATTCTCTAAAGGATGCTTGCACATGAA-ACGATGGGACGAGCTCA 625
Query 359 ACAGTTTCCTAACCGCAACCGGAGCGGTAGTGACATTCGGTTTAAACGCTTTGAGTGGGA 418
||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||
Sbjct 626 ACAGTTTCCTAACCGCAACCGGAGCGGTAGTGACATTCGGTTTAAACGCTTTGCGTGGGA 685
Query 419 GACACAAACTCCGCGGAAAGGCGTGGGGTGGGGCGTGGGATCACATAAACACTCAAGATT 478
||||||||||||||||||||||||||||||| || |||||||||||||||||||||||||
Sbjct 686 GACACAAACTCCGCGGAAAGGCGTGGGGTGGTGCATGGGATCACATAAACACTCAAGATT 745
Query 479 TCATAAACTATACAGTCTCAAAGGGTTACGTTATTGATTCTTGGGAATTCGGTTTCCTTG 538
|| ||||||| |||||||| |||||||||||||||||||||||||||
Sbjct 746 TCTTAAACTACACAGTCTCGAAGGGTTACGTTATTGATTCTTGGGAA------------- 792
Query 539 TTCTCGGTATAGGAAACGAGCTGAGTGGAAGCGGGGTTGGTGCAAGCGTGAGCGCAGAGC 598
| || ||||||||||||||||||||||| |||||||| ||||||||||||||||
Sbjct 793 -T-TC------GGAAACGAGCTGAGTGGAAGCGGAGTTGGTGCGAGCGTGAGCGCAGAGC 844
Query 599 TTTATGGGAAAGACTTGATTGTACTGAAAGATGTAATCAACACAGTTTATAAAAATTCTC 658
|||| |||||||||||||||||||| |||||||||||||||| ||||||||||||||||
Sbjct 845 TTTACGGGAAAGACTTGATTGTACTTAAAGATGTAATCAACAAAGTTTATAAAAATTCTT 904
Query 659 GGTTACACAAGCCTATACTTGTCGCTCCTGGAGGGTTCTATGAACAACAATGGTACACCA 718
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 905 GGTTACACAAGCCTATACTTGTCGCTCCTGGAGGGTTCTATGAACAACAATGGTACACCA 964
Query 719 AACTTCTTCACATCTCCGGTCCTGGTGTTGTCGACGTCGTGACTCATCATATATACAATC 778
|||||||| | || ||||||||| ||||||||||||| ||||||||||||||||||||||
Sbjct 965 AACTTCTTGAAATTTCCGGTCCTAGTGTTGTCGACGTTGTGACTCATCATATATACAATC 1024
Query 779 TTGGTTCAGGTAATGATCCTGCGCTTGTGAAGAAGATAATGGATCCGAGTTACTTAAGCC 838
|||||||||| |||||||| ||||| |||||||||||||||||||||||||| |||||||
Sbjct 1025 TTGGTTCAGGAAATGATCCCGCGCTGGTGAAGAAGATAATGGATCCGAGTTATTTAAGCC 1084
Query 839 AGGTATCAAAAACGTTCAAGGACGTGAATCAGACGATTCAAGAACATGGACGGTGGGCTT 898
||||||||||||| |||||||||||||| |||||||||||||||||||||| ||||||||
Sbjct 1085 AGGTATCAAAAACATTCAAGGACGTGAACCAGACGATTCAAGAACATGGACCGTGGGCTT 1144
Query 899 CTCCTTGGGTTGGAGAGTCTGGTGGAGCTTACAATAGTGGTGGTCGTCATGTTTCTGATA 958
|||||||||||||||| |||||||||||||||||||||||||| |||||||||||||| |
Sbjct 1145 CTCCTTGGGTTGGAGAATCTGGTGGAGCTTACAATAGTGGTGGCCGTCATGTTTCTGACA 1204
Query 959 CATTCATAGATAGTTTCTGGTATCTAGATCAGCTTGGAATGTCGGCGAGATACAACACTA 1018
||||||||||||| ||||||||||||||||||||||||||||| |||||| |||||||||
Sbjct 1205 CATTCATAGATAGCTTCTGGTATCTAGATCAGCTTGGAATGTCAGCGAGACACAACACTA 1264
Query 1019 AAGTTTACTGCAGACAGACTTTGGTTGGAGGGTTTTACGGCTTGCTCGAAAAGGGAACAT 1078
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
Sbjct 1265 AAGTTTACTGCAGACAGACTTTGGTTGGAGGGTTTTACGGCTTGCTCGAAAAGGGAACGT 1324
Query 1079 TTGTTCCAAATCCTGATTACTATAGTGCGCTGCTTTGGCATCGTTTAATGGGAAAAGGGG 1138
||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
Sbjct 1325 TTGTTCCAAATCCTGATTACTATAGCGCGCTGCTTTGGCATCGTTTAATGGGAAAAGGGG 1384
Query 1139 TTCTTGCGGTTCAAACAGATGGACCGCCACAGCTACGAGTTTATGCACATTGTTCAAAAG 1198
|||||||||| || ||||||||||||||||||||||| ||||| ||||||||||||||||
Sbjct 1385 TTCTTGCGGTGCAGACAGATGGACCGCCACAGCTACGGGTTTACGCACATTGTTCAAAAG 1444
Query 1199 GAAGAGCCGGTGTGACATTGCTTCTGATAAATCTAAGCAATCAATTGGATTTTACGGTTA 1258
|||||||||||||||||||||| |||||||||||||||||||||| ||||||||||||||
Sbjct 1445 GAAGAGCCGGTGTGACATTGCTACTGATAAATCTAAGCAATCAATCGGATTTTACGGTTA 1504
Query 1259 GTGTCAGCAATGGCATAAAGGTTGTTTTGAATGCGGAATCGAGCAAGAAGAAGTCATTGT 1318
||||||||||||||||||| || ||||||||||||||||| || ||||||||||||||||
Sbjct 1505 GTGTCAGCAATGGCATAAACGTGGTTTTGAATGCGGAATCAAGGAAGAAGAAGTCATTGT 1564
Query 1319 TGGATACTTTAAAAAGACCATTTTCTTGGATTGGAAGCAAAGCTTCGGATGGATATTTGA 1378
|||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
Sbjct 1565 TGGATACTTTAAAAAGACCATTTTCTTGGATTGGAAGCAAAGCTTCTGATGGATATTTGA 1624
Query 1379 ACAGAGAAGAGTATCATCTGACACCAGAAAACGGTGAGTTGCAG-AGCAAAACAATGATC 1437
| |||||||||||||||||||||||||||||||||| ||||| | |||||||||||| ||
Sbjct 1625 ATAGAGAAGAGTATCATCTGACACCAGAAAACGGTGTGTTGC-GCAGCAAAACAATGGTC 1683
Query 1438 TTGAATGGTAAATCGTTAAAACCGACGGTAACAGGAGATATCCCGAGCCTTGAACCGGTC 1497
|||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 1684 TTGAATGGTAAATCGTTAAAACCGACGGCAACAGGAGATATCCCGAGCCTTGAACCGGTC 1743
Query 1498 CTCCGTGGTGCGAACTCTCCGCTTAATGTCTTGCCATTGTCGATGTCGTTCATTGTGTTG 1557
|||||| ||| ||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1744 CTCCGTAGTGTGAACTCTCCGCTTAATGTCTTGCCATTGTCGATGTCGTTCATTGTGTTA 1803
Query 1558 CCTAATTTTGATGCTTCTGCTTGTTCATGA 1587
||||||||||||||||||||||||||||||
Sbjct 1804 CCTAATTTTGATGCTTCTGCTTGTTCATGA 1833
> AT5G61250.2 | Symbols: AtGUS1, GUS1 | glucuronidase 1 | chr5:24632154-24635303
REVERSE LENGTH=1909
Length=1909
Score = 898 bits (486), Expect = 0.0
Identities = 1241/1589 (78%), Gaps = 118/1589 (7%)
Strand=Plus/Plus
Query 46 CGTCGAGTCTG-TGAGACCGACGAGAACTTTGTCTGTGCCAC-ATTGGACTGGTGGCCTC 103
|||||| || | ||||||||| ||||||||| |||| || || || || |||||||| |
Sbjct 252 CGTCGAATC-GCTGAGACCGATGAGAACTTTATCTGCGCAACGCTT-GATTGGTGGCCAC 309
Query 104 ATGACAAGTGCAACTATGACCAATGTCCTTGGGGTTACTCTTCAGTTATCAATATGGATT 163
||| || ||||||||||| |||||||||||||||||| | || | |||||| || | |
Sbjct 310 CTGAAAAATGCAACTATGATCAATGTCCTTGGGGTTACGCATCTCTCATCAATTTGAACT 369
Query 164 TAAC-TCGTCCCCTTCTTACTAAAGCTATTCAAGCTTTCAAGCCATTGAGGATAAGAATT 222
|| | || ||| |||||| ||||||||||||||||||| | | | ||||||||||||||
Sbjct 370 TAGCTTC-TCCTCTTCTTGCTAAAGCTATTCAAGCTTTTAGGACGTTGAGGATAAGAATA 428
Query 223 GGTGGTTCCTTGCAAGATCAAGT-AACGTACGACGTAGGA-AATCTCAAAACTCCTTGC- 279
||||||||||||||||||||||| | | |||||||| ||| | | | || |||||||||
Sbjct 429 GGTGGTTCCTTGCAAGATCAAGTGATC-TACGACGTTGGAGACT-TGAAGACTCCTTGCA 486
Query 280 CGTCCA-TTCCAA-AAAA-TGAACAG-CGGTTTGTTTGGATTCTCTAAAGGATGCTTGCA 335
| | || || ||| |||| || || | ||| ||||| ||||||||| ||||||| ||| |
Sbjct 487 C-T-CAGTT-CAAGAAAACTG-AC-GACGGATTGTTCGGATTCTCTGAAGGATGTTTGTA 541
Query 336 CATGAAGA-GATGGGACG-AGCTCAA-CAGTTTCCT-AACCGCAACCGGAGCGGTAGTGA 391
||||| | | ||||||| || | || || |||| | || |||||| ||||| | ||||
Sbjct 542 TATGAA-ACGTTGGGACGAAG-TTAACCA-TTTCTTCAA-CGCAACGGGAGCTATTGTGA 597
Query 392 CATTCGGTTTAAACGCTTTG-AGTGGGAGACACAAACTCCGCGGAAAGGCGTGGGGTGGG 450
| |||||||| ||||| || | |||||| | |||||| ||||| |||||||| ||
Sbjct 598 CTTTCGGTTTGAACGCGCTGCA-CGGGAGAAATAAACTCAACGGAACTGCGTGGGGAGGT 656
Query 451 GCGTGGGATCACATAAACACTCAAGATTTCATAAACTATACAGTCTCAAAGGGTTACGTT 510
| |||||||| | |||||||| |||||||| ||||| ||||||||||||||||| | |
Sbjct 657 GATTGGGATCATACCAACACTCAGGATTTCATGAACTACACAGTCTCAAAGGGTTATGCT 716
Query 511 ATTGATTCTTGGGAATTCGGTTTCCTTGTTCTCGGTATAGGAAACGAGCTGAGTGGAAGC 570
|| || || |||| | ||| | || | || ||||| |||||||||
Sbjct 717 ATAGACTCGTGGG-A------------GTT-T-GG--T----AATGAGCTTAGTGGAAGC 755
Query 571 GGG-GTTGGTGCAAGCGTGAGCGCAGAGCTTTATGGGAAAGACTTGATTGTACTGAAAGA 629
||| |||| ||||||||||| | |||||||||||||||||||||||||||||||||| |
Sbjct 756 GGGATTTGG-GCAAGCGTGAGTGTAGAGCTTTATGGGAAAGACTTGATTGTACTGAAAAA 814
Query 630 TGTAATCAA-CACAGTTTATAAAAATTCTCGGTTACACAAGCCTATACTTGTCGCTCCTG 688
||||||||| || ||||| || |||||| | || ||||||| | |||| ||||| |
Sbjct 815 TGTAATCAAGAAC-GTTTACAAGAATTCT-AG-AAC-CAAGCCTTTGGTTGTAGCTCCAG 870
Query 689 GAGGGTTCTATGAACAACAATGGTACACCAAACTTCTTC-ACATCTCCGGTCCTGGTGTT 747
| || |||| |||| ||||||||||| | | ||||||| | |||| || |||||||||
Sbjct 871 GTGGATTCTTTGAAGAACAATGGTACTCGGAGCTTCTTCGGC-TCTCTGGGCCTGGTGTT 929
Query 748 GTCGACGTCGTGACTCATCATATATACAATCTTGGTTCAGGTAATGATCCTGCGCTTGTG 807
| || ||| |||||||||||||||||||||||||| |||| ||||||||| ||| |||
Sbjct 930 CTTGATGTCCTGACTCATCATATATACAATCTTGGTCCAGGGAATGATCCTAAGCTAGTG 989
Query 808 AAGAAGATAATGGATCCGAGTTACTTAAGCCAGGTAT--CAAAAACGTTCAAGGACGTGA 865
|| |||||| | ||||| | |||||| ||| || || | || ||| | | ||||
Sbjct 990 AATAAGATACTAGATCCAAATTACTTGAGC--GGAATTTCGGAATTATTCGCGAATGTGA 1047
Query 866 ATCAGACGATTCAAGAACATGGACGGTGGGCTTCTCCTTGGGTTGGAGA-GTCTGGTGGA 924
| || || |||||||||||||||| |||||| || ||||||||||||| | ||||||||
Sbjct 1048 ACCAAACAATTCAAGAACATGGACCTTGGGCTGCTGCTTGGGTTGGAGAAG-CTGGTGGA 1106
Query 925 GC-TTACAATAGTGGTGGTCGTCATGTTTCTGATACATTCAT-AGATAGTTTCTGGTATC 982
|| || || |||||||| ||||| ||||| || |||||||| | | ||||||||||| |
Sbjct 1107 GCCTTT-AACAGTGGTGGCCGTCAGGTTTCCGAGACATTCATTA-ACAGTTTCTGGTA-C 1163
Query 983 T-AGATCAGCTTGGAATGTCGGCGA-G-ATACAACACTAAAGTTTACTGCAGACA-GACT 1038
| ||||||||| || || ||| ||| | ||| | ||| ||||| ||||||||||| | ||
Sbjct 1164 TTAGATCAGCTCGGTATTTCGTCGAAGCATA-A-CACCAAAGTATACTGCAGACAAG-CT 1220
Query 1039 TTGGTTGGAGGGTTT-TACGG-CTTGCTCGAAAAGGGAACATTTGTTCCAAATCCTGATT 1096
||||| ||||| ||| ||||| || ||| ||||||| ||| |||||||||||||| ||||
Sbjct 1221 TTGGTCGGAGG-TTTCTACGGTCT-GCTTGAAAAGGAAACGTTTGTTCCAAATCCAGATT 1278
Query 1097 ACTATAGTGCGCTGCTTTGGCATCGTTTAATGGGAAAAGGGGTTCTTG-CGGTTCAAACA 1155
|||| || ||||| |||||||||||||| ||||| ||||| |||| | || |||| ||
Sbjct 1279 ACTACAGCGCGCTTCTTTGGCATCGTTTGATGGGTAAAGGCATTCTCGGCG-TTCAGAC- 1336
Query 1156 GA-TGGAC-CGCCAC-AGCTACGAGTTTATGCACATTGTTC-AAAAGGAAGAGCCGGTGT 1211
|| || | || | | ||||||| ||| | ||||| || ||||| |||||| ||| |
Sbjct 1337 GACTGC-CTCGG-AGTATCTACGAGCTTACGTTCATTGCTCCAAAAG-AAGAGCGGGTAT 1393
Query 1212 GAC-ATTGCTTCTGATAAATCTAAGCAATCA-ATTGGAT-TTTACGGTTAGT-GTCAGCA 1267
|| ||| |||||||| ||||| || || || | | | ||||| || | |||||||
Sbjct 1394 AACGATT-CTTCTGATCAATCTGAGTAAGCACAC-G-ACGTTTACAGTC-GCAGTCAGCA 1449
Query 1268 ATGGCATAAAGGTTGTTTTG-AATGCGGAATCGA-GCAAGAAGAAGTCATTGTTGGATAC 1325
||||| | ||||| |||||| || || ||||||| | |||| ||| || || ||||| ||
Sbjct 1450 ATGGCGTGAAGGTGGTTTTGCAA-GCAGAATCGATG-AAGAGGAAATCGTTCTTGGAAAC 1507
Query 1326 TTTAAAAAGACCAT-TTTCTTGGATTGGAAGCAAAGCTTCGGATGGATATTTGAACAGAG 1384
| || || | | |||||||| |||||| |||||||||||| ||||||||||||||||
Sbjct 1508 AATCAAGAG-CAAGGTTTCTTGGGTTGGAAACAAAGCTTCGGACGGATATTTGAACAGAG 1566
Query 1385 AAGAGTATCATCT-GACACCAGAAA-ACGGTGAGTTGCAG-AGCAAAACAATGATCTTGA 1441
|||||||||| || || || ||| ||||||| |||| | ||||| | |||| ||||||
Sbjct 1567 AAGAGTATCA-CTTGAGTCCC-AAAGACGGTGACTTGC-GTAGCAAGATAATGCTCTTGA 1623
Query 1442 ATGGTAAATCGTTAAAACCGACGGTAACAGGAGATATCCCGA-GCCTTGAACCGGTCC-T 1499
|||||||| | || |||||| | || ||||||||||| | || |||| ||||||| |
Sbjct 1624 ATGGTAAACCTTTGGTACCGACAGCCACCGGAGATATCCCAAAGC-TTGAGCCGGTCCGT 1682
Query 1500 CCGTGGTGCGAACTCTCCGCTTAATGTCT-TGCCATTGTCGATGTCGTTCATTGTGTTGC 1558
| ||||| ||| |||||| || || || | || ||||||| || |||||||||||||
Sbjct 1683 CA-TGGTGTGAAATCTCCGGTTTATATCAAT-CCGCTGTCGATCTCTTTCATTGTGTTGC 1740
Query 1559 CTAATTTTGATGCTTCTGCTTGTTCATGA 1587
||| ||||||||| |||||||||| |||
Sbjct 1741 CTACCTTTGATGCTCCTGCTTGTTCTTGA 1769
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 78363050745
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5