BLASTN 2.2.26+


Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.



Database: TAIR10_cdna_20110103_representative_gene_model_updated
           33,602 sequences; 51,074,197 total letters



Query= Ahg359123

Length=1674
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

  AT4G12700.1 | Symbols:  | unknown protein; BEST Arabidopsis tha...  2628    0.0  
  AT2G04280.1 | Symbols:  | unknown protein; FUNCTIONS IN: molecu...  1291    0.0  


> AT4G12700.1 | Symbols:  | unknown protein; BEST Arabidopsis thaliana 
protein match is: unknown protein (TAIR:AT2G04280.1); 
Has 136 Blast hits to 136 proteins in 17 species: Archae - 
0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 125; Viruses 
- 0; Other Eukaryotes - 11 (source: NCBI BLink). | chr4:7482466-7484405 
REVERSE LENGTH=1940
Length=1940

 Score = 2628 bits (1423),  Expect = 0.0
 Identities = 1588/1668 (95%), Gaps = 9/1668 (1%)
 Strand=Plus/Plus

Query  13    TCCAGAACCGGAGGTTTCCGACCAGAGAATCTAGGGCAAAACGCAGTAAGCTTAATCGGA  72
             ||||||||||||||||||||||| ||||||||||||||||||||||||  ||||||||||
Sbjct  99    TCCAGAACCGGAGGTTTCCGACCTGAGAATCTAGGGCAAAACGCAGTATCCTTAATCGGA  158

Query  73    AGCATAGGCTTCTCTATATTCGTAATCGGAGTCGTAGTTTTCACCATTACCGCTGCTACA  132
             ||||||||||||||| || |||||||||||||||||||||||||||||| ||||||||||
Sbjct  159   AGCATAGGCTTCTCTGTACTCGTAATCGGAGTCGTAGTTTTCACCATTATCGCTGCTACA  218

Query  133   TATGAACCAGAAGATCCACTTTTTCACCCATCTGACAAAATCACAACTTTCCTCTCATCC  192
             |||||||||||||||||||| ||||||||||||||||||||||||||||||||| |||||
Sbjct  219   TATGAACCAGAAGATCCACTATTTCACCCATCTGACAAAATCACAACTTTCCTCACATCC  278

Query  193   AATTCAAACGCTACTCTTAAATCCGACGACAGTGTTGTTAAAACCGGTGAAGATTTCATG  252
             ||||||||||||||||||||||||||||||||| |||| |||||||||||||||||||||
Sbjct  279   AATTCAAACGCTACTCTTAAATCCGACGACAGTATTGTCAAAACCGGTGAAGATTTCATG  338

Query  253   GCGGCGAACCAAACCGCG-TTCGCTGGATTCATCAACATAGCTGATGTGGAAACATCTGA  311
             |||||||||||||||||| || || ||||||||||| ||||||||||| |||||||||||
Sbjct  339   GCGGCGAACCAAACCGCGTTTGGC-GGATTCATCAATATAGCTGATGTTGAAACATCTGA  397

Query  312   GAATGATTCTGATGCTAATCAATTGGATTGTGATTCCAATGTCCCAATCGATTGCAAAGA  371
             |||||| ||||||| ||||||||||||||||||| ||||| ||||||| |||||||||||
Sbjct  398   GAATGACTCTGATGGTAATCAATTGGATTGTGATACCAATATCCCAATTGATTGCAAAGA  457

Query  372   TCCTGAAGTTTTTCACTTGATGATGAAAGCTACTATGGAGAAGTTTAAAGACATTCACTT  431
             ||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||
Sbjct  458   TCCTGAAGTTTTTCACTTGATGATGAAAGCTACTATGGAGAAGTTTAAAGACAGTCACTT  517

Query  432   TTATAAGTTTGGTAAACCTGTGACTGTGGAA-G--GTACTAGTTCTTGTGATATGGCTTG  488
             ||||||||||||||||||||||| ||||||| |  ||| |||||||||||||||||||||
Sbjct  518   TTATAAGTTTGGTAAACCTGTGATTGTGGAAGGTAGTAGTAGTTCTTGTGATATGGCTTG  577

Query  489   GCGTTATAGACCTAAGGATGGTAAAACGGCTACGTTTTATAAGGATTATAGGAGGTTTGT  548
             ||||||||||||||||||||||||| ||||| ||||||||||||||||||||||||||||
Sbjct  578   GCGTTATAGACCTAAGGATGGTAAAGCGGCTGCGTTTTATAAGGATTATAGGAGGTTTGT  637

Query  549   GATTGCAAAGTCTGAGAATTGTAGTGTTAGTGTGATGGGTATTGGTGAGTATCATTCGGG  608
             ||||| |||||||| |||||||||||||||||||||||| ||||||||||||||||||||
Sbjct  638   GATTGAAAAGTCTGGGAATTGTAGTGTTAGTGTGATGGGGATTGGTGAGTATCATTCGGG  697

Query  609   TGTGAATGCGAGGAAGCGGAAGAGGCCCGGGTTTCGGAATTCGAGTGGTGGTAAGGTTGA  668
             |||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
Sbjct  698   TGTGAATGCGAGGAAGCGGAAGAGGCCCGGGTTTCGAAATTCGAGTGGTGGTAAGGTTGA  757

Query  669   TGATTTCTCATTGCCTGTGGTTGGTGAAGCAGTGAATGATTCACTTCCTGTGGTTGAGTC  728
             ||||||| |||||||||| |||||||||||||||||||||||||||||||| ||||||||
Sbjct  758   TGATTTCGCATTGCCTGTAGTTGGTGAAGCAGTGAATGATTCACTTCCTGTTGTTGAGTC  817

Query  729   TGAGAATGCGTTTAAAGAAGGTCATTACTTGGTTTATTCGGGTGGAGGAGATAGGTGTAA  788
             ||| |||| |||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct  818   TGAAAATGTGTTTAAAGAAGGTCATTACTTGGTTTATTCTGGTGGAGGAGATAGGTGTAA  877

Query  789   GAGTATGAACCATTTCTTGTGGAGTTTCTTGTGTGCTTTGGGGGAAGCTCAGTATTTGAA  848
             ||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct  878   GAGTATGAACCATTTCTTGTGGAGTTTCTTGTGTGCTTTAGGGGAAGCTCAGTATTTGAA  937

Query  849   TAGGACTTTAGTGATGGATTTGAGTCTTTGTTTGTCTTCTGTTTATACGTTGTCTGGTCA  908
             ||| || |||||||||||||||| |||||||||||||||||||||||| |||||||||||
Sbjct  938   TAGAACATTAGTGATGGATTTGACTCTTTGTTTGTCTTCTGTTTATACATTGTCTGGTCA  997

Query  909   AAACGAGGAAGGAAAGGACTTTAGGTTTTACTTTGATTTCGAGCATTTGAAAGAGGCTGC  968
             |||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  998   AAACGAGGAAGGGAAGGACTTTAGGTTTTACTTTGATTTCGAGCATTTGAAAGAGGCTGC  1057

Query  969   TTCTGTGATAGACCAGGTGCAGTTTTGGGCAGATTGGGGAAAATGGTATAAGAAGAATGG  1028
             |||| || |||||||||||||||||||||| |||||||| ||||||||||||||||||||
Sbjct  1058  TTCTATGTTAGACCAGGTGCAGTTTTGGGCTGATTGGGGGAAATGGTATAAGAAGAATGG  1117

Query  1029  ATTAAAGCTTCATCTTGTGGAAGACTTTAGGGTCACACCGATGAAGCTTGTTGATGTTAA  1088
             |||||||||||||||||||||||| ||| |||||||||||||||||||||||||||| ||
Sbjct  1118  ATTAAAGCTTCATCTTGTGGAAGATTTTCGGGTCACACCGATGAAGCTTGTTGATGTGAA  1177

Query  1089  GGACACGTTGATAATGAGGAAGTTCGGGACAGTAGAACCAGATAACTATTGGTACAGAGT  1148
             |||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
Sbjct  1178  GGACACGTTGATAATGAGGAAGTTCGGGACAGTAGAACCAGATAACTATTGGTATAGAGT  1237

Query  1149  TTGTGAAGGGGAGACAGAATCTGTTGTGCAAAAGCCTTGGCATCTGTTGTGGAAATCTAG  1208
              || |||||||||||||||||||||||||||| ||||||| |||| ||||||||||||| 
Sbjct  1238  ATGCGAAGGGGAGACAGAATCTGTTGTGCAAAGGCCTTGGAATCTATTGTGGAAATCTAA  1297

Query  1209  ACGGCTGATGGAGATTGTTTCTGCGATTGCTTCGAGGTTAAACTGGGATTATGATGCTAT  1268
             ||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  1298  ACGACTGATGGAGATTGTTTCTGCGATTGCTTCGAGGTTAAACTGGGATTATGATGCTAT  1357

Query  1269  TCACATTGAGAGAGGAGATAAGGCGAGAAACAAGGAAGTTTGGCCTAATCTGGAAAAGGA  1328
             |||||||||||||||||| |||||||||||||||||||||||||||||||| ||||||||
Sbjct  1358  TCACATTGAGAGAGGAGACAAGGCGAGAAACAAGGAAGTTTGGCCTAATCTTGAAAAGGA  1417

Query  1329  TACTTCACCAAGCTCCATTTTATCTACCCTGAAGGACAAAATCGAGCAAGGGAGGAATCT  1388
             ||||||||||||||||||||||||||||||  ||||||||||||| ||||| ||||||||
Sbjct  1418  TACTTCACCAAGCTCCATTTTATCTACCCTCCAGGACAAAATCGAACAAGGAAGGAATCT  1477

Query  1389  TTATATTGCAACAAATGAACCAGATGTAA-CTTTCCTTAACCC-TATGAAAGACAAGTAC  1446
             |||||||||||||||||||||||| || | ||||| ||||||| | ||||||||||||||
Sbjct  1478  TTATATTGCAACAAATGAACCAGA-GTTATCTTTCTTTAACCCCT-TGAAAGACAAGTAC  1535

Query  1447  AAAACCCATCTTCTGGATGAGTTTAAGGATCTCTGGGACGAGAGCAGCGAATGGTATTCA  1506
             ||| ||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  1536  AAACCCCATTTTCTGGATGAGTTTAAGGATCTCTGGGACGAGAGCAGCGAATGGTATTCA  1595

Query  1507  GAGACCATGAAGCTTAATGGAGGAAACCCAGTCGATTTTGACGGTTACATGAGAGCGTCT  1566
             ||||| | |||||||||||||||||||||||| || ||||||||||||||||||||||||
Sbjct  1596  GAGACAACGAAGCTTAATGGAGGAAACCCAGTTGAGTTTGACGGTTACATGAGAGCGTCT  1655

Query  1567  GTTGATACGGAAGTGTTCTTGAGAGGGAAGAAGCAGATTGAAACATTCAATGATCTTACC  1626
             |||||||| || ||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  1656  GTTGATACAGAGGTGTTCTTGAGAGGGAAGAAGCAGATTGAAACATTCAATGATCTTACC  1715

Query  1627  AATGACTGTAGAGACGGAATCGGCACTTGCAACGTAGCAGCAAGCTGA  1674
             |||||||||||||| |||||||||||||||||||||||||||||||||
Sbjct  1716  AATGACTGTAGAGATGGAATCGGCACTTGCAACGTAGCAGCAAGCTGA  1763


> AT2G04280.1 | Symbols:  | unknown protein; FUNCTIONS IN: molecular_function 
unknown; INVOLVED IN: biological_process unknown; 
EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 
growth stages; BEST Arabidopsis thaliana protein match is: 
unknown protein (TAIR:AT4G12700.1); Has 130 Blast hits to 130 
proteins in 16 species: Archae - 0; Bacteria - 0; Metazoa 
- 0; Fungi - 0; Plants - 124; Viruses - 0; Other Eukaryotes 
- 6 (source: NCBI BLink). | chr2:1480068-1482089 REVERSE LENGTH=2022
Length=2022

 Score = 1291 bits (699),  Expect = 0.0
 Identities = 1388/1705 (81%), Gaps = 109/1705 (6%)
 Strand=Plus/Plus

Query  22    GGAGGTTTCCGACCAGAGAATCTAGGGCAAAACGCAGTAAGCTTAATCGG-AAGCATAGG  80
             ||||| |||||  ||||||||||||| ||||||||| | | ||||||||| || ||||||
Sbjct  134   GGAGGATTCCGTGCAGAGAATCTAGGTCAAAACGCACTTACCTTAATCGGTAA-CATAGG  192

Query  81    CTTCTCTA-TATTCGTAATCGGAGT-CGTAGTTTTCACCATTACCGCTGCTACATATGAA  138
             |||||| | | |||||  ||||||| | || | |||||||| | ||| || || || |||
Sbjct  193   CTTCTC-ACTCTTCGTCTTCGGAGTAC-TAATCTTCACCATAATCGCCGCAACTTACGAA  250

Query  139   CCAGAAGATCCACTTTTTCACCCATCTGACAAAATCACAACTTTCCTCTCATCCA-ATTC  197
             ||||||||||| || || |||||||||||||||||||||||||||||| | |||| | ||
Sbjct  251   CCAGAAGATCCTCTCTTCCACCCATCTGACAAAATCACAACTTTCCTCACTTCCACA-TC  309

Query  198   AAACGCTACTCTTAAATCCGACGACAGTGTTGTTAAAACCGGTGAAGATTTCATGGC-GG  256
             ||||||||| |||| |||||| |||   |||||||||||||||||||||||||| ||  |
Sbjct  310   AAACGCTACACTTAGATCCGATGACTCCGTTGTTAAAACCGGTGAAGATTTCAT-GCTCG  368

Query  257   CGAACCAAACCGCGTTCGCTGGA-TTCATCAACAT-AGCTGATGTGGAA---A-CATC--  308
             | ||||||||||| || ||  || || |||||||| | | ||||| |||   | || |  
Sbjct  369   CTAACCAAACCGCATTTGC-AGAGTTTATCAACATAAAC-GATGTTGAAGCCAGCACCAA  426

Query  309   TGAGA--A-T---GATTCTGATGCTAATCAA-TTGGATTGTGAT-TCCAATGTC-CCAAT  359
             |||||  | |   ||    || | ||| ||| ||||| |||||| |  |||  | |||||
Sbjct  427   TGAGACTACTACAGAGGAAGAGGGTAA-CAAGTTGGAATGTGATGT-GAAT-ACTCCAAT  483

Query  360   CGATTGCAAAGATCCTG-AAGTTTTTCA-CTTGATGATGAAAGCTACTATGGAGAAGTTT  417
              |||||||||||| | | |||| ||||| ||| ||||||| ||||||||| || ||||||
Sbjct  484   TGATTGCAAAGAT-CAGCAAGTGTTTCATCTT-ATGATGAGAGCTACTATTGACAAGTTT  541

Query  418   AAAGACATTCACTTTTATAAGTTTGGTAAACCTGTGACT-GTG--GAAGGTACT-AGTTC  473
             ||||| ||||||||||||||||||||||| ||||| ||| | |  ||||||  | | |||
Sbjct  542   AAAGATATTCACTTTTATAAGTTTGGTAAGCCTGTTACTGGCGAAGAAGGT-GTGAATTC  600

Query  474   TTGTGATATGGCTTGGCGTTATAGACCTAAG-GATGGTAAAACG-GCTACGTTTTATAAG  531
             |||||||||||| ||||||||||| ||| || ||||| || | | ||| | |||||||||
Sbjct  601   TTGTGATATGGCGTGGCGTTATAGGCCT-AGAGATGGGAAGA-GTGCTGCTTTTTATAAG  658

Query  532   GATTATAGGAGGTTTG-TGATTGCAAAGTCTGAGAATTGTAGTGTTAGTG-TGATGGGTA  589
             |||||||||||||||| || |||| |||||||||||||||||||| |||| || |||| |
Sbjct  659   GATTATAGGAGGTTTGTTG-TTGCTAAGTCTGAGAATTGTAGTGTGAGTGTTGTTGGG-A  716

Query  590   TTGGTGAGTATCATTCGGGTGTGAATGCGAGGAAGCGGAAG-AG-----G---CCCGGGT  640
             |||||||||||||||||||| |||||||||||||| ||||| ||     |    | ||||
Sbjct  717   TTGGTGAGTATCATTCGGGTTTGAATGCGAGGAAGAGGAAGAAGAATCAGAAAGCTGGGT  776

Query  641   TTCG-GAATTCGAGTGGTGGTAAGGTTGATGATTTCTCATTGCCTGTGGTTGGTGAAGC-  698
             || | |||    | |||||| |||   ||||||||||| |||||||| ||||||||| | 
Sbjct  777   TT-GAGAA---AACTGGTGGGAAGAAAGATGATTTCTCTTTGCCTGTTGTTGGTGAA-CT  831

Query  699   AGTGAATGATTCACTTCCTGTGGTTGAGTCTGAGAATGCGTTTAAAGA-AGGTC-ATTAC  756
              ||||||||||| |||||| ||||||||||||| | || |||| ||||  || | | || 
Sbjct  832   TGTGAATGATTCTCTTCCTATGGTTGAGTCTGATAGTGTGTTT-AAGACTGG-CAAGTAT  889

Query  757   TTGGTTTAT-TCGGGTGGAGGAGATAGGTGTAAGAGTATGAACCA-TTTCTTGTGGAGTT  814
             ||||||||| |  ||||| ||||||||||||||||||||||| || |||| |||||||||
Sbjct  890   TTGGTTTATGT-TGGTGGTGGAGATAGGTGTAAGAGTATGAATCATTTTC-TGTGGAGTT  947

Query  815   TCTTGTGTGCT-TTGGGGGAAGCTCAGTATTTGAATAGGACTTTAGTGATGGATTTGAGT  873
             ||||||||||| || ||||||||||||||||||||||||||| |||||||||||||||  
Sbjct  948   TCTTGTGTGCTCTT-GGGGAAGCTCAGTATTTGAATAGGACTCTAGTGATGGATTTGA-C  1005

Query  874   CTT-TGTTTGTCTTCTGTTTATACGTT-GTCTGGTCAAAACGAGGAAGGAAAGGACTTT-  930
             ||| |||||||||||| | ||||| || ||||||||| || |||||||| ||||| ||| 
Sbjct  1006  CTTGTGTTTGTCTTCTATCTATAC-TTCGTCTGGTCAGAATGAGGAAGGCAAGGA-TTTC  1063

Query  931   AGGTTTTACTTTGATTTCGAGCATTTGAAAGAGGCTGCTTCTGTGATAGA-CCAGGTGCA  989
             |||||||||||||||||||||||||||||||| |||||||||||| | ||   || | ||
Sbjct  1064  AGGTTTTACTTTGATTTCGAGCATTTGAAAGAAGCTGCTTCTGTGTTGGATGAAGCT-CA  1122

Query  990   GTTTTGGG--CAGATTGGGGAAAATG-GT-A-TA-AGAAG-AATGGATTAAAGCTTCATC  1042
             ||||||||  ||| | ||||||| || || |  | ||||| ||| |||| || |||||||
Sbjct  1123  GTTTTGGGCTCAG-TGGGGGAAATTGCGTAAGAAGAGAAGAAATAGATTGAACCTTCATC  1181

Query  1043  TTGTGGAAGACTTTAGGGTCACACCGATGAAGCTTGTTGATGTTAAGGACACGTTGATAA  1102
             | ||||| || ||||||||||| || |||||||| |  | ||| || || ||||||||||
Sbjct  1182  TCGTGGAGGATTTTAGGGTCACTCCCATGAAGCTCGCGGCTGTCAAAGATACGTTGATAA  1241

Query  1103  TGAGGAAGTTCGGGA-CAGTAGAACCAGATAACTATTGGTACAGAGTTTGTGAAGGGGA-  1160
             ||||||||||  ||| | ||||||||||| || ||||||||||| ||||||||||| || 
Sbjct  1242  TGAGGAAGTT-TGGATCTGTAGAACCAGACAATTATTGGTACAGGGTTTGTGAAGGAGAT  1300

Query  1161  GACAGAATCTGTTGTGCAAAAG-CCTTGGCATCTGTTGTGGAAATCTAGACGGCTGATGG  1219
             | |||||||||||||  ||| | || ||||||||||| |||||||| ||| || ||||||
Sbjct  1301  G-CAGAATCTGTTGT-TAAACGACCGTGGCATCTGTTATGGAAATCAAGAAGGTTGATGG  1358

Query  1220  AGATTGTTTCTGCGATTGCTTCGAGGTTAAACTGGGATTATGATGCTATTCACATTGAGA  1279
             | || || ||||| ||||| || |||||||||||||| || |||||| | ||||||||||
Sbjct  1359  AAATCGTCTCTGCTATTGCATCAAGGTTAAACTGGGACTACGATGCTGTACACATTGAGA  1418

Query  1280  GAGGAGATAAGGCGAGAAACAAGGAAGTTTGGCCTAATCTGGAAAAGGATACTTCACCAA  1339
             ||||||| || || ||||||||||| |||||||||||||| |||  ||||||||| || |
Sbjct  1419  GAGGAGAGAAAGCAAGAAACAAGGAGGTTTGGCCTAATCTTGAAGCGGATACTTCCCCGA  1478

Query  1340  GCTC-CATTTTATCTACCCTGAAGGACAAAATCG-AGCAAGGGAGGAATCTTTATATTGC  1397
             || | | | || || ||| || |||||||| | | || |||| ||| |||| || || ||
Sbjct  1479  GCGCTC-TCTTGTCGACCTTGCAGGACAAAGTTGAAG-AAGGAAGGCATCTCTACATCGC  1536

Query  1398  AACAAATGAACCAG-ATGTAACTTTCCTT-AACCCTATGAAAGACAAGTACAAAACCCAT  1455
             ||||||||||  || || || | || ||| |||||| ||||||| ||||||   || |||
Sbjct  1537  AACAAATGAAGGAGAAT-TATCCTT-CTTCAACCCTTTGAAAGATAAGTACGCCACTCAT  1594

Query  1456  CTT-CTGGATGAGTTTAAGGATCTCTGGGACGAGAGCAGCGAATGGTATTCAGAGACCA-  1513
              || ||  | || |  || ||||| ||||| |||||||| || |||||||||||||| | 
Sbjct  1595  -TTCCTTTACGACTACAAAGATCTTTGGGATGAGAGCAGTGAGTGGTATTCAGAGACTAC  1653

Query  1514  TGAAGCTTAATGGAGGAAACCCAGTCGATTTTGACGGTTACATGAGAGCGTCTGTTGATA  1573
             | |||||||||||||| ||||| ||||| ||||| |||||||||||||| |||||||| |
Sbjct  1654  T-AAGCTTAATGGAGGCAACCCGGTCGAATTTGATGGTTACATGAGAGCATCTGTTGACA  1712

Query  1574  CGGAAGTGTTCTTGAGAGGGAAGAAGCAGATTGAAACATTCAATGATCTTACCAATGACT  1633
             | ||||| ||||||||||||||||||||||||||||||||||| |||||||| || || |
Sbjct  1713  CAGAAGTATTCTTGAGAGGGAAGAAGCAGATTGAAACATTCAACGATCTTACAAACGATT  1772

Query  1634  GTAGAGACGGAATCGGCACTTGCAA  1658
             ||| ||| ||| | || || |||||
Sbjct  1773  GTAAAGATGGAGTTGGGACCTGCAA  1797



Lambda     K      H
    1.33    0.621     1.12 

Gapped
Lambda     K      H
    1.28    0.460    0.850 

Effective search space used: 82730498160


  Database: TAIR10_cdna_20110103_representative_gene_model_updated
    Posted date:  Sep 25, 2014  6:13 PM
  Number of letters in database: 51,074,197
  Number of sequences in database:  33,602



Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5