BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg474176
Length=2154
Score E
Sequences producing significant alignments: (Bits) Value
AT1G50480.1 | Symbols: THFS | 10-formyltetrahydrofolate synthet... 3413 0.0
AT2G12280.1 | Symbols: | P-loop containing nucleoside triphosp... 388 9e-107
AT2G12200.1 | Symbols: | P-loop containing nucleoside triphosp... 255 9e-67
AT2G12230.1 | Symbols: | pseudogene, C-1-tetrahydrofolate synt... 128 2e-28
AT1G50490.1 | Symbols: UBC20 | ubiquitin-conjugating enzyme 20 ... 115 2e-24
> AT1G50480.1 | Symbols: THFS | 10-formyltetrahydrofolate synthetase
| chr1:18701951-18704938 FORWARD LENGTH=2269
Length=2269
Score = 3413 bits (1848), Expect = 0.0
Identities = 2070/2174 (95%), Gaps = 28/2174 (1%)
Strand=Plus/Plus
Query 1 AGAGAGAGCGATGAGTTCATCATCAAGAAAGCTTGAGCTTGTTTCACCAGTTCCGGCCGA 60
|||||| ||||||||||||||| |||||||||||||| |||||||||||||||| |||||
Sbjct 104 AGAGAGTGCGATGAGTTCATCAACAAGAAAGCTTGAGGTTGTTTCACCAGTTCCCGCCGA 163
Query 61 TATCGATATAGCTAATTCCGTCGAGCCTTTACACATCTCTGAGATTGCCAAAGATCTTAA 120
|||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 164 TATCGACATAGCTAATTCCGTCGAGCCTTTACACATCTCTGAGATTGCCAAAGATCTTAA 223
Query 121 TATCAACCCACTTCACTACGATCTCTATGGCAAGTATAAAGCTAAGGTTTTGTTGTCTGC 180
||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 224 TATCAACCCTCTTCACTACGATCTCTATGGCAAGTATAAAGCTAAGGTTTTGTTGTCTGC 283
Query 181 GTTTGATGAGCTTCAAGGACAAGAAGATGGTTACTATGTTGTTGTTGGAGGGATTACTCC 240
|||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
Sbjct 284 GTTTGATGAGCTTCAAGGACAAGAAGATGGATACTATGTTGTTGTTGGAGGGATTACTCC 343
Query 241 TACTCCTCTTGGAGAAGGCAAGAGTACTACCACTGTTGGACTTTGCCAAGCCTTAGGCGC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 344 TACTCCTCTTGGAGAAGGCAAGAGTACTACCACTGTTGGACTTTGCCAAGCCTTAGGCGC 403
Query 301 TTACCTTGATAAGAAGGTTGTTACTTGTCTTCGCCAACCGTCACAAGGACCCACCTTTGG 360
|||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 404 TTACCTCGATAAGAAGGTTGTTACTTGTCTTCGCCAACCGTCACAAGGACCCACCTTTGG 463
Query 361 AATCAAAGGAGGTGCAGCTGGTGGTGGGTATAGTCAGGTCATTCCTATGGATGAGTTCAA 420
||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 464 AATCAAAGGAGGTGCAGCTGGTGGTGGGTATAGTCAGGTGATTCCTATGGATGAGTTCAA 523
Query 421 TCTTCATCTCACTGGAGATATTCACGCGATCACCGCTTCCAACAATCTCTTAGCTGCTGC 480
|||||||||||||||||||||||||||||| |||||||||||||| ||||||||||||||
Sbjct 524 TCTTCATCTCACTGGAGATATTCACGCGATTACCGCTTCCAACAACCTCTTAGCTGCTGC 583
Query 481 AATCGACACTCGGATTTTCCATGAGACGTCTCAATCCGACAAGGCGCTTTTTAATAGGTT 540
||||||||||||||||||||||||||||||||||||||||||||| |||||||||||| |
Sbjct 584 AATCGACACTCGGATTTTCCATGAGACGTCTCAATCCGACAAGGCTCTTTTTAATAGGCT 643
Query 541 GTGCCCTCCGAATAAAGAAGGGAAGCGTAGTTTCAGTGACATTATGTTCAGGAGATTGAC 600
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 644 GTGCCCTCCGAATAAAGAAGGGAAGCGTAGTTTCAGTGACATTATGTTCAGGAGATTGAC 703
Query 601 AAAGCTTGGGATATCCAAGACCACTCCTGAGGAGCTTACTCCCGAGGAGATAAAGAAATT 660
||||||||||||||||||||||| ||||||||||||||||||||||||||||||||| ||
Sbjct 704 AAAGCTTGGGATATCCAAGACCAGTCCTGAGGAGCTTACTCCCGAGGAGATAAAGAAGTT 763
Query 661 TGCTAGGCTTGATATCGATCCTGCTTCTATCACGTGGAGAAGAGTTATGGATGTTAATGA 720
||| ||||||||||| ||||||||||||||||| ||||||||||| ||||||||||||||
Sbjct 764 TGCGAGGCTTGATATTGATCCTGCTTCTATCACTTGGAGAAGAGTGATGGATGTTAATGA 823
Query 721 TCGGTTCTTGAGGAAGATTACTATTGGACAGGGACCTGAGGAAAAGGGGATGACTAGAGA 780
||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||
Sbjct 824 TCGGTTCTTGAGGAAGATTACTATTGGTCAGGGACCTGAGGAAAAGGGGATGACTAGAGA 883
Query 781 AACTGGTTTTGACATTTCTGTTGCTAGTGAGATTATGGCTGTTTTGGCTTTGACAACCTC 840
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 884 AACTGGTTTTGACATTTCTGTTGCTAGTGAGATTATGGCTGTTTTGGCTTTGACAACCTC 943
Query 841 TCTAGGTGATATGAGAGAGAGGCTAGGTAAAATGGTGATTGGAAACAGCAAAGCCGGGGA 900
||||||||||||||||||||||| ||||||||||||||||| |||||||| || |||||
Sbjct 944 ACTAGGTGATATGAGAGAGAGGCTTGGTAAAATGGTGATTGGTAACAGCAAGGCAGGGGA 1003
Query 901 TCCGATAACAGCAGATGATCTTGGTGTTGGAGGAGCCTTGACTGTTCTGATGAAAGACGC 960
||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
Sbjct 1004 TCCGATAACAGCAGATGATCTCGGTGTTGGAGGAGCCTTGACTGTTCTGATGAAAGACGC 1063
Query 961 TATTAACCCAACACTCATGCAGACACTGGAAGGGACACCTGTCCTTGTTCATGCTGGTCC 1020
||||||||| |||||||||||||||||||||||||| ||||| || || |||||||||||
Sbjct 1064 TATTAACCCGACACTCATGCAGACACTGGAAGGGACCCCTGTTCTAGTGCATGCTGGTCC 1123
Query 1021 TTTTGCAAACATAGCTCATGGAAATTCATCGGTTGTTGCTGATAAAATCGCTTTGAAGCT 1080
||||||||| ||||||||||||||||||||| ||||||||||||||||||||||||||||
Sbjct 1124 TTTTGCAAATATAGCTCATGGAAATTCATCGATTGTTGCTGATAAAATCGCTTTGAAGCT 1183
Query 1081 GGTGGGACCTGGTGGATTCGTGGTAACAGAAGCGGGTTTTGGTTCTGATATTGGAACAGA 1140
|||||||||||||||||| |||||||| ||||||||||||||||||||||||||||||||
Sbjct 1184 GGTGGGACCTGGTGGATTTGTGGTAACTGAAGCGGGTTTTGGTTCTGATATTGGAACAGA 1243
Query 1141 GAAGTTCATGAATATTAAGTGCCGCTACAGCGGGCTAACACCTCAGTGTGCGATTGTTGT 1200
|||||||||||||||||||||||| ||||| |||||||| ||||||||||| ||||||||
Sbjct 1244 GAAGTTCATGAATATTAAGTGCCGTTACAGTGGGCTAACGCCTCAGTGTGCAATTGTTGT 1303
Query 1201 GGCGACTGTTAGGGCCTTGAAAATGCATGGTGGTGGGCCTGATGTTGTTGCCGGGAGGCC 1260
||||||||||||||| |||||||||||||| |||||||||||||||||||||||||| ||
Sbjct 1304 GGCGACTGTTAGGGCTTTGAAAATGCATGGAGGTGGGCCTGATGTTGTTGCCGGGAGACC 1363
Query 1261 ACTTGATCGTGCTTATGTAAGCGAGAATGTTTCCTTGGTTGAAGCTGGCTGTGTGAATCT 1320
||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
Sbjct 1364 TCTTGATCGTGCTTATGTAAGCGAGAATGTTTCCTTAGTTGAAGCTGGCTGTGTGAATCT 1423
Query 1321 GGCAAAGCACATCTCAAACACAAAGGCCTACGGTGTGAATGTAGTTGTTGCTGTGAATAT 1380
||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
Sbjct 1424 GGCAAAGCACATCTCAAACACAAAGGCCTACGGTGTGAATGTAATTGTTGCTGTGAATAT 1483
Query 1381 GTTCTCAACAGATACCGAAGCAGAACTAAATGCAGTTAGGAAATTTTCAATGGATGCTGG 1440
|||| |||||||||||||||||||||||||||||||||||||||||||||||||||| ||
Sbjct 1484 GTTCGCAACAGATACCGAAGCAGAACTAAATGCAGTTAGGAAATTTTCAATGGATGCCGG 1543
Query 1441 TGCTTTTGATGCGGTGGTCTGCTCCCACCATGCTCATGGTGGCAAAGGAGCGGTGGATCT 1500
|||||||||||| |||||||||||||||||||||||| |||| |||||||||||||||||
Sbjct 1544 TGCTTTTGATGCTGTGGTCTGCTCCCACCATGCTCATAGTGGTAAAGGAGCGGTGGATCT 1603
Query 1501 TGGTATTGCGGTTGAAAAAGCTTGTCAAAACATTACACAGCCACTTAGGTTTCTCTACCC 1560
|||||| ||||||||||||||||||||||||||||||||||| || ||||||||||||||
Sbjct 1604 TGGTATCGCGGTTGAAAAAGCTTGTCAAAACATTACACAGCCCCTCAGGTTTCTCTACCC 1663
Query 1561 ATTGGACATTAGTATCAAAGACAAAATTGAGGCCATAGCTAAGTCATATGGAGCCAGTGG 1620
|||||||||| |||||||||||||||||||||| ||||||||||||||||||||||||||
Sbjct 1664 ATTGGACATTGGTATCAAAGACAAAATTGAGGCAATAGCTAAGTCATATGGAGCCAGTGG 1723
Query 1621 TGTTGAATACTCAGAACAGGCAGAGAAACAGATTGAGATGTACACACAACAAGGCTTCTC 1680
||||||||| ||||| ||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1724 TGTTGAATATTCAGACCAGGCAGAGAAACAGATTGAGATGTACACACAACAAGGCTTCTC 1783
Query 1681 GAATCTTCCCATATGCATGTCGAAAACACAGTACTCATTCTCGCATGATGCATCAAAGAA 1740
|||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
Sbjct 1784 GAATCTTCCCATATGCATGTCGAAAACACAGTACTCATTCTCACATGATGCATCAAAGAA 1843
Query 1741 AGGAGCACCTTCGGGGTTTGTTTTGCCAATAAGGGATGTAAGAGGAAGCATCGGAGCTGG 1800
|||||||||||| |||||||| |||||||| |||||||||||||||||||| ||||||||
Sbjct 1844 AGGAGCACCTTCAGGGTTTGTATTGCCAATTAGGGATGTAAGAGGAAGCATTGGAGCTGG 1903
Query 1801 TTTCATATATCCACTGGTTGGTACAATGAGTACAATGCCTGGACTTCCTACAAGACCTTG 1860
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1904 TTTCATATATCCACTGGTTGGTACAATGAGTACAATGCCTGGACTTCCTACAAGACCTTG 1963
Query 1861 TTTCTACGAGATTGATATTGACACTGAAACTGGAAAAGTTCGTGGCCTTTCTTAAATTTG 1920
||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
Sbjct 1964 TTTCTACGAGATTGATATTGACACTGAAACTGGAAAAGTTCGTGGCCTTTCTTAAGTTTG 2023
Query 1921 GTGGATCGATCAGTCAAAGCTTCAAG-CTT-CCTTCACCATCATTTCCCTCTTCAACAAT 1978
|| |||| | |||||||||||||| ||| |||||| | || |||| | |||| |||||
Sbjct 2024 GTAGATC--T--GTCAAAGCTTCAAGGCTTTCCTTCATCGTCGTTTCTCACTTCCACAAT 2079
Query 1979 AAAAATTTGGCGATTGTACTT-GATATTGAGTGTTTTTCCATCTTTAA-ATGAATAA-AA 2035
||||||||||||||||||||| |||| ||||||||||||||| ||||| |||||||| ||
Sbjct 2080 AAAAATTTGGCGATTGTACTTTGATACTGAGTGTTTTTCCAT-TTTAAGATGAATAATAA 2138
Query 2036 TAAAAGTATCTTTATCATCT-GATTAATCTCTGAAA-A--ACT-A--A------CTCAAG 2082
|||| |||||||| || | | ||||||||||||||| | ||| | | ||||||
Sbjct 2139 TAAA-GTATCTTT-TCCTTTTGATTAATCTCTGAAATACTACTCATTATTTGAACTCAAG 2196
Query 2083 GTAAAGCTACAGA--GAACTTCGGATGTTCAAAAACAAAGAATAAAAGCAAATGAATGAC 2140
|||| | |||||| ||||||| ||||||||||||||||||||||| |||||||||||||
Sbjct 2197 GTAACGTTACAGATAGAACTTCTGATGTTCAAAAACAAAGAATAAA-GCAAATGAATGAC 2255
Query 2141 AGTGTTTTCTTCTG 2154
||||||||||||||
Sbjct 2256 AGTGTTTTCTTCTG 2269
> AT2G12280.1 | Symbols: | P-loop containing nucleoside triphosphate
hydrolases superfamily protein | chr2:4922282-4922679
FORWARD LENGTH=398
Length=398
Score = 388 bits (210), Expect = 9e-107
Identities = 232/243 (95%), Gaps = 0/243 (0%)
Strand=Plus/Plus
Query 1636 ACAGGCAGAGAAACAGATTGAGATGTACACACAACAAGGCTTCTCGAATCTTCCCATATG 1695
||||||| | ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 21 ACAGGCACATAAACAGATTGAGATGTACACACAACAAGGCTTCTCGAATCTTCCCATATG 80
Query 1696 CATGTCGAAAACACAGTACTCATTCTCGCATGATGCATCAAAGAAAGGAGCACCTTCGGG 1755
|||||||||||| |||||||||||||| ||||||||||||||||||||||||||||| ||
Sbjct 81 CATGTCGAAAACGCAGTACTCATTCTCACATGATGCATCAAAGAAAGGAGCACCTTCAGG 140
Query 1756 GTTTGTTTTGCCAATAAGGGATGTAAGAGGAAGCATCGGAGCTGGTTTCATATATCCACT 1815
|||||| |||||||| ||||||| ||||||||||| |||||||||||||||||||||||
Sbjct 141 GTTTGTATTGCCAATTCGGGATGTTAGAGGAAGCATTGGAGCTGGTTTCATATATCCACT 200
Query 1816 GGTTGGTACAATGAGTACAATGCCTGGACTTCCTACAAGACCTTGTTTCTACGAGATTGA 1875
||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
Sbjct 201 GGTTGGTACAATGAGTACAATGCCTGGACTTCCTACAAGACCTTGTTTCTATGAGATTGA 260
Query 1876 TAT 1878
|||
Sbjct 261 TAT 263
> AT2G12200.1 | Symbols: | P-loop containing nucleoside triphosphate
hydrolases superfamily protein | chr2:4900613-4900944
FORWARD LENGTH=230
Length=230
Score = 255 bits (138), Expect = 9e-67
Identities = 162/174 (93%), Gaps = 0/174 (0%)
Strand=Plus/Plus
Query 1636 ACAGGCAGAGAAACAGATTGAGATGTACACACAACAAGGCTTCTCGAATCTTCCCATATG 1695
||||||| | ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 17 ACAGGCACATAAACAGATTGAGATGTACACACAACAAGGCTTCTCGAATCTTCCCATATG 76
Query 1696 CATGTCGAAAACACAGTACTCATTCTCGCATGATGCATCAAAGAAAGGAGCACCTTCGGG 1755
|||||||||||| |||||||||||||| |||||| ||||||||||| |||||||||| ||
Sbjct 77 CATGTCGAAAACGCAGTACTCATTCTCACATGATACATCAAAGAAAAGAGCACCTTCAGG 136
Query 1756 GTTTGTTTTGCCAATAAGGGATGTAAGAGGAAGCATCGGAGCTGGTTTCATATA 1809
|||||| |||||||| ||||||| ||||||||||| |||||||||||||||||
Sbjct 137 GTTTGTATTGCCAATTCGGGATGTTAGAGGAAGCATTGGAGCTGGTTTCATATA 190
> AT2G12230.1 | Symbols: | pseudogene, C-1-tetrahydrofolate synthase,
blastp match of 91% identity and 2.9e-07 P-value to
GP|9843502|emb|CAC03667.1||AL133260 dJ292B18.2 (novel protein
similar to methylenetetrahydrofolate dehydrogenase (NADP+
dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate
synthetase (MTHFD1) (EC 1.5.1.5, EC 3.5.4.9,
EC-TRUNCATED- | chr2:4909261-4909488 FORWARD LENGTH=228
Length=228
Score = 128 bits (69), Expect = 2e-28
Identities = 71/72 (99%), Gaps = 0/72 (0%)
Strand=Plus/Plus
Query 1807 ATATCCACTGGTTGGTACAATGAGTACAATGCCTGGACTTCCTACAAGACCTTGTTTCTA 1866
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 22 ATATCCACTGGTTGGTACAATGAGTACAATGCCTGGACTTCCTACAAGACCTTGTTTCTA 81
Query 1867 CGAGATTGATAT 1878
|||||||||||
Sbjct 82 TGAGATTGATAT 93
> AT1G50490.1 | Symbols: UBC20 | ubiquitin-conjugating enzyme 20
| chr1:18704805-18706478 REVERSE LENGTH=918
Length=918
Score = 115 bits (62), Expect = 2e-24
Identities = 76/82 (93%), Gaps = 3/82 (4%)
Strand=Plus/Minus
Query 2075 AACTCAAGGTAAAGCTACAG--AGAACTTCGGATGTTCAAAAACAAAGAATAAAAGCAAA 2132
|||||||||||| | ||||| |||||||| ||||||||||||||||||||||| |||||
Sbjct 865 AACTCAAGGTAACGTTACAGATAGAACTTCTGATGTTCAAAAACAAAGAATAAA-GCAAA 807
Query 2133 TGAATGACAGTGTTTTCTTCTG 2154
||||||||||||||||||||||
Sbjct 806 TGAATGACAGTGTTTTCTTCTG 785
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 106826759760
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5