BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg474726
Length=2163
Score E
Sequences producing significant alignments: (Bits) Value
AT1G54510.1 | Symbols: ATNEK1, NEK1 | NIMA-related serine/threo... 3094 0.0
AT3G63280.1 | Symbols: ATNEK4, NEK4 | NIMA-related kinase 4 | c... 425 7e-118
> AT1G54510.1 | Symbols: ATNEK1, NEK1 | NIMA-related serine/threonine
kinase 1 | chr1:20358319-20362545 REVERSE LENGTH=2254
Length=2254
Score = 3094 bits (1675), Expect = 0.0
Identities = 1908/2020 (94%), Gaps = 17/2020 (1%)
Strand=Plus/Plus
Query 1 CAAATTTGTCATCTTCGTCCCCACAAATCACTCTCCCAAATAGCTTTCTTCTCTTACCAT 60
|||||||||||||||||||||||| |||||||||| ||| || ||||||||| || || |
Sbjct 1 CAAATTTGTCATCTTCGTCCCCACTAATCACTCTCGCAAGTATCTTTCTTCT-TT-CC-T 57
Query 61 T-AACTCTTAAACTCTCGCCATCAGATC--TTTCTCTGATTTCTTGGGTGAGAAAAT-A- 115
| |||||||||||||||||||||||||| ||||||||||||||||||||||||||| |
Sbjct 58 TAAACTCTTAAACTCTCGCCATCAGATCTTTTTCTCTGATTTCTTGGGTGAGAAAATAAC 117
Query 116 AAA---AGCTAGAAATGGAACAGTATGAGATTCTTGAGCAAATTGGAAAAGGATCTTTTG 172
||| ||||||||||||||||||||||| ||||||||||||||||||||||||||||||
Sbjct 118 AAAGCTAGCTAGAAATGGAACAGTATGAGTTTCTTGAGCAAATTGGAAAAGGATCTTTTG 177
Query 173 GGTCAGCTCTTTTAGTGAGACATAAGCATGAGAAGAAAAAATATGTATTGAAAAAGATCC 232
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 178 GGTCAGCTCTTTTAGTGAGACATAAGCATGAGAAGAAAAAATATGTATTGAAAAAGATCC 237
Query 233 GTCTTGCCCGGCAAACTCAGAGGACCAGACGGTCTGCACATCAAGAGATGGAGCTTATCT 292
||||||||||||||||||| ||||||||||| ||||||||||||||||||||||||||||
Sbjct 238 GTCTTGCCCGGCAAACTCAAAGGACCAGACGATCTGCACATCAAGAGATGGAGCTTATCT 297
Query 293 CGAAAATGCGTCATCCATTTATTGTGGAATACAAAGATTCTTGGGTGGAAAAGGCTTGCT 352
|||||||||||||||||||||| |||||||||||||| ||||||||||||||||||||||
Sbjct 298 CGAAAATGCGTCATCCATTTATCGTGGAATACAAAGACTCTTGGGTGGAAAAGGCTTGCT 357
Query 353 ATGTGTGCATCGTCATAGGTTATTGTGAAGGAGGGGACATGGCACAAGCTATCAAGAAAT 412
|||||||||| ||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 358 ATGTGTGCATTGTCATAGGTTATTGTGAAGGAGGAGACATGGCACAAGCTATCAAGAAAT 417
Query 413 CCAATGGCATGTATTTTTC-GGAAGAGAAACTGTGCAAGTGGCTTGTTCAGCTTCTGATG 471
||||||| || ||||| | ||||||||||||||||||||||||||||||||| ||||||
Sbjct 418 CCAATGGTGTGCATTTT-CAGGAAGAGAAACTGTGCAAGTGGCTTGTTCAGCTCCTGATG 476
Query 472 GGCCTTGAGTACTTACACTCTAACCATATTCTTCATCGTGATGTCAAGTGTTCTAACATA 531
||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
Sbjct 477 GGCCTTGAGTACTTACATTCTAACCATATTCTTCATCGTGATGTCAAGTGTTCTAACATA 536
Query 532 TTCTTGACCAAAGAGCAAGATATACGCCTTGGTGATTTTGGTTTAGCCAAGATTCTAACA 591
||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
Sbjct 537 TTCTTGACCAAAGAGCAAGATATACGCCTTGGTGATTTTGGGTTAGCCAAGATTCTAACA 596
Query 592 TCCGACAATCTCACTTCCTCCGTTGTCGGGACTCCCAGCTATATGTGCCCTGAGCTTCTA 651
|||||| ||||||| ||||| |||||||||||||||||||||||||||||||||||| ||
Sbjct 597 TCCGACGATCTCACATCCTCTGTTGTCGGGACTCCCAGCTATATGTGCCCTGAGCTTTTA 656
Query 652 GCTGATATACCTTATGGTTCTAAATCTGACATTTGGTCTTTAGGATGCTGTATATATGAA 711
|||||||||||||||||||| ||||||||||||||||||||||| |||||||||||||||
Sbjct 657 GCTGATATACCTTATGGTTCCAAATCTGACATTTGGTCTTTAGGGTGCTGTATATATGAA 716
Query 712 ATGGCTTATCTTAAGCCGGCATTTAAAGCATTTGATATGCAAGCACTGATTAATAAGATA 771
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 717 ATGGCTTATCTTAAGCCGGCATTTAAAGCATTTGATATGCAAGCACTGATTAATAAGATA 776
Query 772 AACAAGACGATTGTGGCTCCGCTCCCTGCAAAATATTCTGGTTCATTCCGAAGTCTTGTC 831
||||||||||||||| ||||||||||||| |||||||||||| |||||||| |||| |||
Sbjct 777 AACAAGACGATTGTGTCTCCGCTCCCTGCCAAATATTCTGGTCCATTCCGAGGTCTCGTC 836
Query 832 AAAAGCATGCTGCGAAAAAACCCAGAAGTTCGGCCATCTGCCTCAGATTTACTTAGACAT 891
|||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 837 AAAAGCATGCTGCGGAAAAACCCAGAAGTTCGGCCATCTGCCTCAGATTTACTTAGACAT 896
Query 892 CCACATCTTCAGCCTTATGTTCTGGATGTCAAACTCAGGCTCAATAACCTGAGGCGCAAA 951
|||||||||||||||||||||||||||||||| || ||||||||||| || || ||||||
Sbjct 897 CCACATCTTCAGCCTTATGTTCTGGATGTCAAGCTTAGGCTCAATAATCTTAGACGCAAA 956
Query 952 ACATTACCGCCTCAATGGCC-AGATTCTAAGAGGATTATGAAGAGAGCTCATTTTTCTGA 1010
|||||||||||| | | ||| || ||| |||||||||||||||| |||||||||||||||
Sbjct 957 ACATTACCGCCTGAGTTGCCGAG-TTCCAAGAGGATTATGAAGAAAGCTCATTTTTCTGA 1015
Query 1011 ACCCGCTGTCACTTGTCCAGCCTTTGGAGAACGACAACATAGTTCTCTATGGAATGACAG 1070
||| |||||||||||||||||||||||||||||||||||||| ||||| |||||||||||
Sbjct 1016 ACCAGCTGTCACTTGTCCAGCCTTTGGAGAACGACAACATAGGTCTCTCTGGAATGACAG 1075
Query 1071 AGCTTTGAATCCCGAAGCTGAAGAAGACACTGCATCTTCTATCAAATGTATAAGTCGCAG 1130
|||| ||||||| ||||||||||||||||||||||||||||| |||||||| ||||||||
Sbjct 1076 AGCTCTGAATCCAGAAGCTGAAGAAGACACTGCATCTTCTATTAAATGTATTAGTCGCAG 1135
Query 1131 AATTACAGATTTATCAATTGAGAGCAGCAGTAAAAGAACAGTGATATGTAAGCAAGTTGC 1190
|||| |||||||||| |||||||||||||||||| ||||| ||||||||||||||||| |
Sbjct 1136 AATTTCAGATTTATCGATTGAGAGCAGCAGTAAAGGAACATTGATATGTAAGCAAGTTTC 1195
Query 1191 TTCAAGTGCTTCTAAGGTCTCAAAGTATCCTCTTGCAAAATCTTCAGTGACATCCAGAAG 1250
||||||||| | ||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1196 TTCAAGTGCATGTAAGGTCTCAAAGTATCCTCTTGCAAAATCTTCAGTGACATCCAGAAG 1255
Query 1251 AATCATGGAAACTGGAAGGATATTCGCAC-ACTTGCATCCGGTTTCAGGTGGTGGTACTA 1309
|||||||||||||||| ||| || || || |||||||||||||||||||||||||||| |
Sbjct 1256 AATCATGGAAACTGGACGGAGATCCG-ACCACTTGCATCCGGTTTCAGGTGGTGGTACCA 1314
Query 1310 CCAGCAAGATAATTCCATCAGCTCGCCGGACTTCTCTACCACTGACGAAAAGGGCTACAA 1369
||||||||||||||||||||||||||||||||||||||||||| |||||||| |||||||
Sbjct 1315 CCAGCAAGATAATTCCATCAGCTCGCCGGACTTCTCTACCACTCACGAAAAGAGCTACAA 1374
Query 1370 ACCAAGAAGCTGCAGCTTACAACCGAACAGTTGGAATCTTGCAGAATGTGAAGTCTCCAG 1429
||||||||| |||||||||||||| || |||||| |||||||||||||| ||||||||||
Sbjct 1375 ACCAAGAAGTTGCAGCTTACAACCCAATAGTTGGGATCTTGCAGAATGTCAAGTCTCCAG 1434
Query 1430 AATATTCCATTAATGAGCCACAAGTTGATAAGATTGCCATTTTCCCATTAGCTCCATATG 1489
||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
Sbjct 1435 AATATTCCATTAATGAGCCACAAGTTGATAAGATTGCCATTTTTCCATTAGCTCCATATG 1494
Query 1490 AACAAGACATCTTCTTTACTCCCATGCAAAGGAGAACATCTTCCAAAAGCTCCTCTGTCT 1549
||||||||||||||||||||||||||||||||| |||||| || ||||||||||||||||
Sbjct 1495 AACAAGACATCTTCTTTACTCCCATGCAAAGGAAAACATCGTCTAAAAGCTCCTCTGTCT 1554
Query 1550 CAGACCGCTCTATAACAAAAGACAAATGCACAGTTCAAACTCATACGACGTGGCAGGGAA 1609
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1555 CAGACCGCTCTATAACAAAAGACAAATGCACAGTTCAAACTCATACGACGTGGCAGGGAA 1614
Query 1610 TCCAACTCAACATGGTAGACAACATAAGCGAAGGGTCATCATCATCTGATCAGAATGCAA 1669
||||||||||||||||||| ||||||||||| ||||||||||||||||||||||||||||
Sbjct 1615 TCCAACTCAACATGGTAGATAACATAAGCGACGGGTCATCATCATCTGATCAGAATGCAA 1674
Query 1670 CTGCTGGTGTATCAAGCCAGACCACATCCTCTACTACAAGAAGATGTCGGTTTGACCCAT 1729
| ||||||| ||||||||| ||||||||||||| | ||||||||||||||||||||||||
Sbjct 1675 CAGCTGGTGCATCAAGCCACACCACATCCTCTAGTTCAAGAAGATGTCGGTTTGACCCAT 1734
Query 1730 CATCGTATCGTCAACGGGCTGATGCTTTGGAGGGGTTACTTGAGTTTAGTGCCAGATTAT 1789
||||||||||||||||||||||||||||||||||||| |||||||||||||||||||| |
Sbjct 1735 CATCGTATCGTCAACGGGCTGATGCTTTGGAGGGGTTGCTTGAGTTTAGTGCCAGATTGT 1794
Query 1790 TACAAGAAGGGAGATATGATGAACTCAACGTGTTGCTTAAGCCTTTTGGCCCAGGTAAGG 1849
|||||||||||||||||||||||||||| || ||||||||||||||||| ||||| ||||
Sbjct 1795 TACAAGAAGGGAGATATGATGAACTCAATGTATTGCTTAAGCCTTTTGGTCCAGGAAAGG 1854
Query 1850 TGTCTCCCAGAGAAACCGCTATATGGATCGCAAAGAGCTTGAAGGAGAATCGTGAGAAGT 1909
||||||||||||||||||| |||||||||||||||||||||||||||||||| || |||
Sbjct 1855 TGTCTCCCAGAGAAACCGCGATATGGATCGCAAAGAGCTTGAAGGAGAATCGAGACAAGA 1914
Query 1910 CAAAAACGGTAAATCTAAATGTTTCTAGAGAGATACCACCTGTGGGTCTCCTCTGACAGG 1969
| |||| |||| ||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 1915 CCAAAATGGTAGATCTAAATGTTTCTAGAGAGATACCACATGTGGGTCTCCTCTGACAGG 1974
Query 1970 CTTCACTTTGTCGTAACGTCCAGTTTAGTACGGTTTTAGC 2009
|| ||| ||||| |||||| | ||||||| ||||||||||
Sbjct 1975 CTCCACATTGTCATAACGTTCGGTTTAGTTCGGTTTTAGC 2014
Score = 167 bits (90), Expect = 5e-40
Identities = 149/174 (86%), Gaps = 17/174 (10%)
Strand=Plus/Plus
Query 2007 AGCTAGAGTTGGTTTA-G----GTTTGATGGCTGACACTTTTAAAAGATGAGTAACAGTA 2061
|||||||||||||||| | ||||||||||||||||||||||||||||||||||||||
Sbjct 2063 AGCTAGAGTTGGTTTAGGTTTAGTTTGATGGCTGACACTTTTAAAAGATGAGTAACAGTA 2122
Query 2062 TTAGTAGG---------ATGGTCTTTGCTTGAGCCGAACCAGTAGCTTTGCATATGTAAT 2112
|||||||| ||||||||||||| || |||||| |||||||||||| ||||||
Sbjct 2123 TTAGTAGGTTGCGTTGGATGGTCTTTGCTTTAGTCGAACCGGTAGCTTTGCATTTGTAAT 2182
Query 2113 TTGTACACTT-A--GTGTTGACACGTTTTATAACTTGTATTAATATAGACTTCA 2163
||||||||| | ||||| |||| ||||||||||||||||| |||||||||||
Sbjct 2183 CTGTACACTTGATAGTGTTAACACCTTTTATAACTTGTATTACTATAGACTTCA 2236
> AT3G63280.1 | Symbols: ATNEK4, NEK4 | NIMA-related kinase 4 |
chr3:23377979-23381546 FORWARD LENGTH=2066
Length=2066
Score = 425 bits (230), Expect = 7e-118
Identities = 621/805 (77%), Gaps = 46/805 (6%)
Strand=Plus/Plus
Query 127 ATGGAACAGTATGAGATTCTT-GAGCAAATTGGAAAAGGATCTTTTGGGTCAGCTCTT-T 184
||||| | |||||| | |||| ||||| || || ||||| || ||||| || |||||| |
Sbjct 215 ATGGAGCGGTATGA-AGTCTTGGAGCAGATCGGGAAAGGGTCCTTTGGCTCTGCTCTTCT 273
Query 185 TAGTGAGACATAAGCATGAGAA-G-AAAAAATATGTATTGAAAAAGATCCGTCTTGCCCG 242
| || ||||| || || |||| | || ||||| || ||||||||||| ||||| |||||
Sbjct 274 T-GTTAGACACAAACA--AGAACGCAAGAAATACGTTTTGAAAAAGATTCGTCTCGCCCG 330
Query 243 GCAAACTCAGAGGAC-CAGACGGTCTGCACATCAAGAGATGGAGCTTATCTCGAAAATGC 301
|| || | ||| | | |||| || || ||||| ||||||||||| || || | | |
Sbjct 331 CCAGTCTGATAGGGCTC-GACGATCAGCTCATCAGGAGATGGAGCTCATTTCTACTGTCC 389
Query 302 GTC-ATCCATTTATTGTGGAATACAAAGATTCTTGGGTGGAAAAGGCTTGCTATGTGTGC 360
| | |||||||| |||| || ||||||||||| ||||| ||||||| |||||||||||||
Sbjct 390 G-CAATCCATTTGTTGTCGAGTACAAAGATTCATGGGTTGAAAAGGGTTGCTATGTGTGC 448
Query 361 ATCGTCATAGGTTATTGTGAAGGAGGGGACATGGCACA-AGCTATCAAGAA-ATCCAATG 418
|| || || || ||||| |||| ||||||||| || | | | ||||| || | | |||
Sbjct 449 ATTGTTATTGGATATTGCCAAGGCGGGGACATGACAGATA-CCATCAA-AAGA-GC-ATG 504
Query 419 -GCATG-T-ATTTTTCGGAAGAGAAACTGTG-CAAGTGGCTTGTTCAGCT-TCTGATGGG 473
| || | ||||| | || |||||| | || ||| |||||||| || || | | ||||
Sbjct 505 TG-GTGTTCATTTTCCTGAGGAGAAATTATGCCAA-TGGCTTGTCCAACTAT-TAATGGC 561
Query 474 CCTTGAGTA-CTTACACTCTAACCATATTCTTCATCGTGATGTCAAGTGTTCTAACATAT 532
||| || || ||| ||||| |||||||||||||| || ||||||||||||||||||||||
Sbjct 562 CCTCGATTATCTT-CACTCCAACCATATTCTTCACCGCGATGTCAAGTGTTCTAACATAT 620
Query 533 TCTTGACCAAAGAGCAAGATATACGCCTTGGTGATTTTGGTTTAGCCAAGATTCTAACAT 592
||||||| ||||||||||| ||||| |||||||| ||||| | || ||||||||||| |
Sbjct 621 TCTTGACAAAAGAGCAAGACATACGGCTTGGTGACTTTGGACTCGCTAAGATTCTAACTT 680
Query 593 CCGACAATCTCACTTCCTCCGTTGTCGGGACTCCCAGCTATATGTGCCCTGAGCTTCTAG 652
| || | || || ||||| ||||| || ||||| ||||| ||||| ||||||||||| |
Sbjct 681 CTGATGACCTTACATCCTCTGTTGTTGGAACTCCAAGCTACATGTGTCCTGAGCTTCTTG 740
Query 653 CTGATATACCTTATGGTTCTAAA-TCTGACATTTGGTCTTTAGGATGCTGTATATATGAA 711
|||| |||||||| || || ||| || ||||||||||| || |||||||| || ||||||
Sbjct 741 CTGACATACCTTACGGATC-AAAGTCAGACATTTGGTCGTTGGGATGCTGCATGTATGAA 799
Query 712 ATGGCTTATCTTAAGCCGGCATTT-AAAGCATTT-GATATGCA-AGCACTGATTAATAAG 768
|||||| || ||| || | ||| ||||| || ||| |||| | ||||||| | ||
Sbjct 800 ATGGCTGCTCATAAACCTCC-TTTCAAAGC-TTCCGATGTGCAGA-CACTGATCACCAAA 856
Query 769 ATAAACAAGACGATTGTGGCTCCGCT-CCCTGCAAAATATTCTGGTTCATTCCGAAGTCT 827
||| ||||| ||| ||| ||| | ||| || | ||||| || ||||||||| | ||
Sbjct 857 ATACACAAGTTGATAATGGATCCTATTCCC-GCCATGTATTCCGGCTCATTCCGAGGCCT 915
Query 828 TGTCAAAAGCATGCTGCGAAAAAACCCAGAAGTTC-GGCCAT-CTGCCTCA-GATTTACT 884
| | ||||||||| || | ||||| || ||| | | ||||| | || | | || | ||
Sbjct 916 TATTAAAAGCATGTTGAGGAAAAATCCTGAACT-CAGGCCAAGC-GC-TAACGAGCTGCT 972
Query 885 TAGAC-ATCCACATCTTCAGCCTTA 908
|| || |||| ||||||||||||||
Sbjct 973 TA-ACCATCCTCATCTTCAGCCTTA 996
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 107278564665
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5