BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg477525
Length=1649
Score E
Sequences producing significant alignments: (Bits) Value
AT3G02830.1 | Symbols: ZFN1 | zinc finger protein 1 | chr3:6138... 2357 0.0
AT5G16540.1 | Symbols: ZFN3 | zinc finger nuclease 3 | chr5:540... 416 3e-115
> AT3G02830.1 | Symbols: ZFN1 | zinc finger protein 1 | chr3:613802-616144
FORWARD LENGTH=1695
Length=1695
Score = 2357 bits (1276), Expect = 0.0
Identities = 1426/1494 (95%), Gaps = 27/1494 (2%)
Strand=Plus/Plus
Query 1 TGGAGGTGAGACGAGAACGCttttttgctttttCGTCTTtctctctctctctctctcttt 60
|||||||||||||||||||||||||||| |||||||| || ||||||||||| | |||||
Sbjct 1 TGGAGGTGAGACGAGAACGCTTTTTTGC-TTTTCGTC-TT-TCTCTCTCTCT-T-TCTTT 55
Query 61 cttcactaccctctcacctctctgtttacacga------------------ttcttc-tt 101
|||||||| |||||||||||||||||||||||| |||||| ||
Sbjct 56 CTTCACTA-CCTCTCACCTCTCTGTTTACACGATTCTTCTTCTTCTTCTTCTTCTTCTTT 114
Query 102 agatgtcttcttCCTCCATTGTCATTTACCTATCTACACTTCATTTTCTTGTCGGAGGAA 161
||||||||||||||||||||||||| ||||| |||||||||||||||||||| ||| |||
Sbjct 115 AGATGTCTTCTTCCTCCATTGTCATCTACCTCTCTACACTTCATTTTCTTGTTGGAAGAA 174
Query 162 GATCGATTATCGGTGATTCATTCTTAGTGATGACAGTATAATGTCTGATGCTTatcatca 221
||||||||| |||||| |||||||||||||||||||||||||||||||||||||| ||||
Sbjct 175 GATCGATTACCGGTGACTCATTCTTAGTGATGACAGTATAATGTCTGATGCTTATTATCA 234
Query 222 tcatcatcatcatcGGAGACATTCTTCCTCTTCACGTCTATGGATTTTAATGCCGGAGTT 281
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 235 TCATCATCATCATCGGAGACATTCTTCCTCTTCACGTCTATGGATTTTAATGCCGGAGTT 294
Query 282 CCCATGTCTTCTCTTTCACCTTTAATGAATCAAGATGCAATGTGGCAAATGAATTTGAGT 341
||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
Sbjct 295 CCCATGTCTTCTCTTTCACCTTTGATGAATCAAGATGCAATGTGGCAAATGAATTTGAGT 354
Query 342 TCAGATGAAACAATGGAAACTGGTTCCTACCCTGAACGACCAGGAGAGCCTGATTGTTCT 401
|||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||
Sbjct 355 TCAGATGAAACAATGGAAACTGGTTCTTACCCTGAACGACCAGGAGAGCCTGATTGTTCT 414
Query 402 TATTACATAAGAACTGGACTTTGTAGATTTGGCTCTACTTGTCGGTTCAATCATCCTCGT 461
||||||||||||||||| |||||||||||||||||||||||||||||||| |||||||||
Sbjct 415 TATTACATAAGAACTGGTCTTTGTAGATTTGGCTCTACTTGTCGGTTCAACCATCCTCGT 474
Query 462 GATCGGGAACTGGTTATAGCCACTGCAAGGATGAGAGGAGAGTACCCTGAAAGGATTGGT 521
|||||||||||||||||||| |||||||||||||||||||| ||||||||||||||||||
Sbjct 475 GATCGGGAACTGGTTATAGCTACTGCAAGGATGAGAGGAGAATACCCTGAAAGGATTGGT 534
Query 522 CAGCCTGAATGCGAGTACTATTTGAAGACAGGAACCTGCAAGTTTGGAGTAACATGTAAG 581
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 535 CAGCCTGAATGCGAGTACTATTTGAAGACAGGAACCTGCAAGTTTGGAGTAACATGTAAG 594
Query 582 TTTCATCATCCTAGGAACAAAGCTGGTATTGCTGGAAGAGTCTCTCTCAATATGTTAGGC 641
||||||||||| |||||||||||||| |||||||||||||||||||||||||||||||||
Sbjct 595 TTTCATCATCCAAGGAACAAAGCTGGGATTGCTGGAAGAGTCTCTCTCAATATGTTAGGC 654
Query 642 TATCCTCTCCGCTCGAATGAAGTTGATTGTGCTTATTTTCTAAGAACCGGACATTGTAAA 701
|||||||| ||||||||||| ||||||||||||||||| |||||||||||||| ||||||
Sbjct 655 TATCCTCTACGCTCGAATGAGGTTGATTGTGCTTATTTCCTAAGAACCGGACACTGTAAA 714
Query 702 TTTGGTGGTACTTGTAAATTCAACCACCCTCAGCCTCAACCAACCAACATGATGGTTCCA 761
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 715 TTTGGTGGTACTTGTAAATTCAACCACCCTCAGCCTCAACCAACCAACATGATGGTTCCT 774
Query 762 ACTTCAGGCCAACAGTCTTATCCTTGGTCAAGAGCTTCTTTTATTGCCAGCCCTCGTTGG 821
|| ||||| |||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 775 ACCTCAGGGCAACAGTCTTATCCTTGGTCAAGAGCTTCTTTTATTGCCAGCCCTCGTTGG 834
Query 822 CAAGATCCGTCTAGCTACGCTTCCTTAATCATGCCTCAAGGAGTTGTCCCGGTTCAAGGA 881
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 835 CAAGATCCGTCTAGCTACGCTTCCTTAATCATGCCTCAAGGAGTTGTCCCGGTTCAAGGA 894
Query 882 TGGAACCCTTACAGTGGTCAGCTCGGCTCAGTTTCTCCTTCAGGTACCGGAAATGATCAC 941
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 895 TGGAACCCTTACAGTGGTCAGCTCGGCTCAGTTTCTCCTTCAGGTACCGGAAATGATCAA 954
Query 942 AACTACAGAAACATACAGCAGAACGAGACAATAGAGTCAGGCTCTCAGTCTCAAGGGTCA 1001
||||||||||| ||||||||||||||||||||||||||||||||||||||||||| |||
Sbjct 955 AACTACAGAAATTTACAGCAGAACGAGACAATAGAGTCAGGCTCTCAGTCTCAAGGATCA 1014
Query 1002 TTCTCGGGTTTCAATCCGGGATCCTCTGTTCCGATAGGAGGGTATTATGCATTGCCGAGG 1061
|||||||||| |||||||||||||||||||||| ||||||||||||||||||||||||||
Sbjct 1015 TTCTCGGGTTACAATCCGGGATCCTCTGTTCCGCTAGGAGGGTATTATGCATTGCCGAGG 1074
Query 1062 GAAAATGTGTTCCCGGAAAGACCAGGACAACCCGAATGCCAATTCTACATGAAGACAGGT 1121
||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
Sbjct 1075 GAAAATGTGTTCCCGGAGAGACCAGGACAACCCGAATGCCAATTCTACATGAAGACAGGT 1134
Query 1122 GACTGCAAATTTGGCACAGTTTGCAAGTTTCACCATCCTAGAGACAGACAAGCTCCTCCT 1181
|||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||
Sbjct 1135 GACTGCAAATTTGGCACAGTTTGCAAATTTCACCATCCTAGAGACAGACAAGCTCCTCCT 1194
Query 1182 CCTGATTGTCTCTTAAGCTCCATTGGCCTCCCTTTACGCCCGGGAGAACCGTTGTGTGTG 1241
|||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
Sbjct 1195 CCTGATTGTCTCTTAAGCTCCATTGGCCTCCCCTTACGCCCGGGAGAACCGTTGTGTGTG 1254
Query 1242 TTCTATACTCGATATGGAATCTGCAAATTTGGTCCAAGCTGTAAATTTGATCACCCAATG 1301
|||||||| || ||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1255 TTCTATACACGCTATGGAATCTGCAAATTTGGTCCAAGCTGTAAATTTGATCACCCAATG 1314
Query 1302 AGAGTATTCGCATACGAGAACACAGCTTCAGAGACAGATGAGGTAGTGGAAACATCAACA 1361
|||||||| ||||||| ||||||||||||||||||||||||||||||||||||||||||
Sbjct 1315 CGAGTATTCACATACGACAACACAGCTTCAGAGACAGATGAGGTAGTGGAAACATCAACA 1374
Query 1362 GGGCAATCCAGAAGACACTCTGTATCTGAAACAAGACAAGCAGCAACAACATCCTCTGGT 1421
||| |||| ||||||| |||||| ||||||||||||||||||||||||||||||||||||
Sbjct 1375 GGGAAATCTAGAAGACTCTCTGTGTCTGAAACAAGACAAGCAGCAACAACATCCTCTGGT 1434
Query 1422 AAAGATACTACCATTGATAATACACAGCAGTGAAGAAACAAA--AAGAAGAAGA 1473
||||| |||||||||||||||||||||||||||||||||||| ||||||||||
Sbjct 1435 AAAGACACTACCATTGATAATACACAGCAGTGAAGAAACAAAGGAAGAAGAAGA 1488
Score = 305 bits (165), Expect = 7e-82
Identities = 171/174 (98%), Gaps = 0/174 (0%)
Strand=Plus/Plus
Query 1476 TTTGATTTTTCAAAAGAGGAATCTGTAAATTGTCATTTTCATTTAGTCTCTCAAGCATTT 1535
||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||
Sbjct 1522 TTTGATTTTTCAAAAGAGGAATCTGTAAATTCTCATTTTCATTTAGTCTCTCAAGCATTT 1581
Query 1536 ATTAATAATGTATAGAGGTGATTTATACAACTGATCTTGATCTTGATCTGATGGCCATTG 1595
|||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
Sbjct 1582 ATTAATAATGTATAGAGGTGATTTATACAACTGATCTTGATCTTGATCTGATCGCCATTG 1641
Query 1596 TTGTAATATTCTTTTGGCGAATCAGGTTGAATAAAATTGAAAGTAATTTCCCTC 1649
|||||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 1642 TTGTAATATTCTTTTGGCGAATCAGGTTCAATAAAATTGAAAGTAATTTCCCTC 1695
> AT5G16540.1 | Symbols: ZFN3 | zinc finger nuclease 3 | chr5:5403188-5405154
FORWARD LENGTH=1497
Length=1497
Score = 416 bits (225), Expect = 3e-115
Identities = 396/478 (83%), Gaps = 13/478 (3%)
Strand=Plus/Plus
Query 289 CTTCTCTTTCACCTTTAATGAATCAAGATGCAATGTGGCAAATGAATTTGAGTTCAGATG 348
||| |||||| || || | || ||| ||||||||||||||||||| ||| | |||||||
Sbjct 281 CTTTTCTTTCCCCATTGCTTAACCAAAATGCAATGTGGCAAATGAACTTGGGATCAGATG 340
Query 349 AAACAAT--G-G-AAACTGGTTCCTACCCTGAACGACCA-GGAGAGCCTGATTGTTCTTA 403
| ||||| | | | ||| || || || ||||| || |||||||| |||||| ||||
Sbjct 341 ATACAATGGGTGTTGA-TGGGTCTTATCCGGAACG-TCACGGAGAGCCAGATTGTGCTTA 398
Query 404 TTACATAAGAACTGGACTTTGTAGATTTGGCTCTACTTGTCGGTTCAATCATCCTCGTGA 463
||| ||||| |||||||||||||| ||||| || ||||| || ||||||||||||| ||
Sbjct 399 TTATATAAGGACTGGACTTTGTAGGTTTGGTTCAACTTGCCGATTCAATCATCCTCACGA 458
Query 464 TCGGGAACTGGTTATAGCCACTGCAAGGATGAGAGGAGAGTACCCTGAAAGGATTGGTCA 523
||| ||||||||||||| ||||||||||| | |||||| ||||||||||| ||||||||
Sbjct 459 CCGGAAACTGGTTATAGCAACTGCAAGGATTAAAGGAGAATACCCTGAAAGAATTGGTCA 518
Query 524 GCCTGAATGCGAGTACTATTTGAAGACAGGAACCTGCAAGTTTGGAGTAACATGTAAGTT 583
||||||||| |||| ||| |||||||||| |||||||| || ||||| || || |||||
Sbjct 519 GCCTGAATGTGAGTTTTATCTGAAGACAGGGACCTGCAAATTCGGAGTGACTTGCAAGTT 578
Query 584 TCATCATCCTAGGAACAAAGCTGGTATTGCTGGAAGAGTCTCTCTCAATATGTTAGGCTA 643
||||||||||||||||||||||||||||| |||||| || || | ||| | ||| ||||
Sbjct 579 TCATCATCCTAGGAACAAAGCTGGTATTGATGGAAGTGTATCAGTTAATGTCTTAAGCTA 638
Query 644 TCCTCTCCGCTC-GAATGAAGTTGATTG-TGCTTA-TTTTCTAAGAACCGGACATTGTAA 700
|||||| || || ||| |||| |||||| | |||| |||| | |||| ||||| |||||
Sbjct 639 TCCTCTACG-TCCGAACGAAGATGATTGCT-CTTACTTTT-TGAGAATTGGACAATGTAA 695
Query 701 ATTTGGTGGTACTTGTAAATTCAACCACCCTCAGCCTCAACCAACCAACATGATGGTT 758
|||||| || |||||||||||||| ||||||||| ||||| |||||||| ||||||||
Sbjct 696 ATTTGGGGGAACTTGTAAATTCAATCACCCTCAGACTCAATCAACCAACTTGATGGTT 753
Score = 329 bits (178), Expect = 4e-89
Identities = 273/319 (86%), Gaps = 6/319 (2%)
Strand=Plus/Plus
Query 1006 CGGGTTTCAATCCGGGATCCTCTGTTCCGATAGGAGGGTAT-TATGCATTGCCGAGGGAA 1064
|||||||| || ||||| ||||||||| | |||| | |||||||| || || ||
Sbjct 906 CGGGTTTC-ATTCGGGAAATTCTGTTCCGCT---CGGGT-TCTATGCATTACCTAGAGAG 960
Query 1065 AATGTGTTCCCGGAAAGACCAGGACAACCCGAATGCCAATTCTACATGAAGACAGGTGAC 1124
||||| ||||||||||||||||| ||||| |||||||||||||||||||||||||| ||
Sbjct 961 AATGTATTCCCGGAAAGACCAGGCCAACCTGAATGCCAATTCTACATGAAGACAGGAGAT 1020
Query 1125 TGCAAATTTGGCACAGTTTGCAAGTTTCACCATCCTAGAGACAGACAAGCTCCTCCTCCT 1184
||||||||||| ||||| ||||| ||||| |||||||||||||| ||| | |||||||||
Sbjct 1021 TGCAAATTTGGAACAGTCTGCAAATTTCATCATCCTAGAGACAGGCAAACCCCTCCTCCT 1080
Query 1185 GATTGTCTCTTAAGCTCCATTGGCCTCCCTTTACGCCCGGGAGAACCGTTGTGTGTGTTC 1244
|||||| |||||||||| ||||||| |||||||||||||||||||| |||| ||||||
Sbjct 1081 GATTGTGTCTTAAGCTCGGTTGGCCTTCCTTTACGCCCGGGAGAACCACTGTGCGTGTTC 1140
Query 1245 TATACTCGATATGGAATCTGCAAATTTGGTCCAAGCTGTAAATTTGATCACCCAATGAGA 1304
||| |||| ||||| |||||||| ||||||||||||||||||||||||||||| ||| ||
Sbjct 1141 TATTCTCGGTATGGGATCTGCAAGTTTGGTCCAAGCTGTAAATTTGATCACCCGATGCGA 1200
Query 1305 GTATTCGCATACGAGAACA 1323
|| ||| | ||| | ||||
Sbjct 1201 GTCTTCACTTACAACAACA 1219
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 81475484535
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5