BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg478365
Length=1861
Score E
Sequences producing significant alignments: (Bits) Value
AT3G10350.2 | Symbols: | P-loop containing nucleoside triphosp... 1105 0.0
AT3G10360.1 | Symbols: APUM4, PUM4 | pumilio 4 | chr3:3210884-3... 486 3e-136
> AT3G10350.2 | Symbols: | P-loop containing nucleoside triphosphate
hydrolases superfamily protein | chr3:3208264-3210931
FORWARD LENGTH=1601
Length=1601
Score = 1105 bits (598), Expect = 0.0
Identities = 733/793 (92%), Gaps = 29/793 (4%)
Strand=Plus/Plus
Query 770 AATTGTGTTTGATACTGCACCAACGGGTCATACGCTAAGGCTACTCTCTTTACCTGATTT 829
||| || |||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 811 AATCGTATTTGATACTGCACCAACGGGTCATACGCTAAGGCTACTCTCTTTACCTGATTT 870
Query 830 CTTGGATGCGTCCATTGGTAAAATCTTGAAGCTTAGACAGAAAATAACCTCAGCTACTTC 889
||||||||| ||||||||||||||||||||||||||||||||||||||||| ||||||||
Sbjct 871 CTTGGATGCATCCATTGGTAAAATCTTGAAGCTTAGACAGAAAATAACCTCGGCTACTTC 930
Query 890 AGCGATCAAATCAGTCTTTGGTAAAGAAGAAAATCGGCCTGATGCTGCCGACAAATTGGA 949
||||||||||||||| ||||| ||||||||||| |||||||||||||||||||||||||
Sbjct 931 AGCGATCAAATCAGTTTTTGGGAAAGAAGAAAAGGGGCCTGATGCTGCCGACAAATTGGA 990
Query 950 ACGACTAAGAGAAAGGATGGTTAAAGTTCGCGAGCTTTTCCGTGACACAGAGTCAACAGA 1009
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 991 AAAACTAAGAGAAAGGATGGTTAAAGTTCGCGAGCTTTTCCGTGACACAGAGTCAACAGA 1050
Query 1010 ATTCGTCATTGTCACTATCCCAACGGTAATGGCTGTCAGCGAGTCTTCTAGGTTAAGTGC 1069
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1051 ATTCGTCATTGTCACTATCCCAACGGTAATGGCTGTCAGCGAGTCTTCTAGGTTAAGTGC 1110
Query 1070 TTCCTTGAAGAAGGAAAGTGTTCCCGTCAAAAGACTCATTGTTAATCAGATTCTCCCGCC 1129
||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
Sbjct 1111 TTCCTTGAAGAAGGAAAGTGTTCCCGTCAAAAGACTCATTGTTAATCAGCTTCTCCCGCC 1170
Query 1130 ATCCTCCTCAGACTGCAAGTTTTGCTCAATTAAGAGAAAGGATCAAATGCGGGCTCTTGA 1189
|||||||||||| ||||| |||||||| |||||||||||||| |||||||||||||||||
Sbjct 1171 ATCCTCCTCAGATTGCAAATTTTGCTCTATTAAGAGAAAGGACCAAATGCGGGCTCTTGA 1230
Query 1190 TATGATCCGGGAGGATTCCGAACTCTCTGCTTTGACACTGATGGAGGCTCCACTGGTTGA 1249
|||||||||||||||||| |||||||||||||||||||||||||||||||| ||||||||
Sbjct 1231 TATGATCCGGGAGGATTCAGAACTCTCTGCTTTGACACTGATGGAGGCTCCGCTGGTTGA 1290
Query 1250 TATGGAGATCAGAGGTGTCCCTGCTCTGCGCTTCTTGGGTGATATCATTTGGAAATAAGA 1309
||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 1291 TATGGAGATCAGAGGTGTCCCTGCTCTGCGCTTCTTGGGCGATATCATTTGGAAATAAGA 1350
Query 1310 TCATTACACATTATCAACACAAGGATGAAGAACAAGCAGGTTCACTTGTATGAGATAGT- 1368
|||||||| ||||||| ||||||||||||||||||||||||||||||||||||||||||
Sbjct 1351 TCATTACATATTATCACCACAAGGATGAAGAACAAGCAGGTTCACTTGTATGAGATAGTG 1410
Query 1369 TT--TT-------------G-----G-TACAGATGTTGATATT-GTCCTTGATGGGTTTG 1406
|| || | | || |||||||||| || ||| ||||||||||||
Sbjct 1411 TTGGTTAATACATTACATAGTTTTTGGTATAGATGTTGATGTTTGTCTTTGATGGGTTTG 1470
Query 1407 GTCTTTGGG-AAAACATGAATATTGACAAGTGTTCCAAACTGTTAACAAATTCAGTATTT 1465
|||||||| |||| |||||||| ||| ||| || ||||||||||||| |||||||||||
Sbjct 1471 GTCTTTGGCTAAAA-ATGAATATCGACGAGTTTTTCAAACTGTTAACAGATTCAGTATTT 1529
Query 1466 ACTACATTTTGGTGGGTGTAAAATGTTCAAT-TAAAACTTTAGTA-CTTTTAAAATAAAA 1523
|||||||||||||||||||||||||||| | ||||||||||| | ||||||||||||||
Sbjct 1530 GCTACATTTTGGTGGGTGTAAAATGTTCAGTGTAAAACTTTAG-AGCTTTTAAAATAAAA 1588
Query 1524 A-TATAGCAATTC 1535
| |||||||||||
Sbjct 1589 AATATAGCAATTC 1601
Score = 774 bits (419), Expect = 0.0
Identities = 452/468 (97%), Gaps = 1/468 (0%)
Strand=Plus/Plus
Query 259 TTCAAGTGAAATCAGTAGCTAGCCCAACGGAGACGATTTCGGAATTCGAAGAGATGGTCT 318
||| |||||||||||||||||||||||||||||||||||| |||||||| ||||||||||
Sbjct 328 TTC-AGTGAAATCAGTAGCTAGCCCAACGGAGACGATTTCAGAATTCGATGAGATGGTCT 386
Query 319 CCGGTACTAAACGAAAGTATTACATGCTCGGTGGGAAAGGAGGAGTGGGCAAAACTAGTT 378
|||| || ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 387 CCGGCACGAAACGAAAGTATTACATGCTCGGTGGGAAAGGAGGAGTGGGCAAAACTAGTT 446
Query 379 GTGCTGCTTCTTTAGCTGTCAGATTCGCCAACAATGGACACCCAACTCTTGTTGTGTCTA 438
||||||||||||||||||| ||||||||||||||||||||||||||||| ||||| ||||
Sbjct 447 GTGCTGCTTCTTTAGCTGTGAGATTCGCCAACAATGGACACCCAACTCTGGTTGTATCTA 506
Query 439 CAGATCCAGCACATTCCTTGAGCGATTCTTTTGCTCAGGACTTGACAGGAGGAATGCTTG 498
|||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
Sbjct 507 CAGATCCAGCACATTCCTTGAGCGATTCTTTTGCTCAGGACTTGACTGGAGGAATGCTTG 566
Query 499 TCCCTGTAGAAGGACCTGAAGCTCCTTTATTTGCTCTAGAAATAAACCCTGAGAAGGCTA 558
|||||||||||||||| |||||||||||||||||| ||||||||||||||||||||||||
Sbjct 567 TCCCTGTAGAAGGACCCGAAGCTCCTTTATTTGCTTTAGAAATAAACCCTGAGAAGGCTA 626
Query 559 GGGAAGAGTTTCGTAGTGCCTCGCAGATGAATGGAGGAACTGGGATGAAAGATTTTATGG 618
|||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||
Sbjct 627 GGGAAGAGTTTCGTAGTGCCTCGCAGATGAATGGAGGAACTGGGGTGAAAGATTTTATGG 686
Query 619 ATGGTATGGGACTTGGGATGCTTGTAGAACAGTTGGGGGAGCTAAAACTTGGAGAATTGC 678
|||||||||||||||| |||||||||||||||||||||||||| ||||||||||||||||
Sbjct 687 ATGGTATGGGACTTGGAATGCTTGTAGAACAGTTGGGGGAGCTTAAACTTGGAGAATTGC 746
Query 679 TAGACACACCGCCTCCTGGATTGGATGAAGCCATTGCCATTTCTAAGG 726
| |||||||||||||||||||||||||||||||| |||||||||||||
Sbjct 747 TCGACACACCGCCTCCTGGATTGGATGAAGCCATAGCCATTTCTAAGG 794
Score = 318 bits (172), Expect = 1e-85
Identities = 196/207 (95%), Gaps = 4/207 (2%)
Strand=Plus/Plus
Query 62 AGCAGACG-AAAAAATGGCGACTCTGTCTTCCTACCTATTATCTTCTCCTCCTCTATGCA 120
|||||||| ||||||||||||||||||||||||| || ||||||||||||||||||||||
Sbjct 33 AGCAGACGAAAAAAATGGCGACTCTGTCTTCCTATCTGTTATCTTCTCCTCCTCTATGCA 92
Query 121 AATCCCGATTCTCGGCAACAAGTCTCGTCTCGGGAATTGATTTCATCTCTTTCTCCCCAC 180
||||||||||||||||||||||||||||||| ||||||||||||||||||||||| || |
Sbjct 93 AATCCCGATTCTCGGCAACAAGTCTCGTCTCAGGAATTGATTTCATCTCTTTCTCTCCTC 152
Query 181 GAACAACTCTCTCCTC---ATCCACTGTATTGCCGGCGATTCTTTCTCTTTCCGTTAAAT 237
|||||||||||||||| ||||||||| ||||||||||||||||||||||||||||||
Sbjct 153 GAACAACTCTCTCCTCGTCATCCACTGTCTTGCCGGCGATTCTTTCTCTTTCCGTTAAAC 212
Query 238 ATAATCGCCGGAGAAACTCGCTTCAAG 264
|||||||||||||||||||||||||||
Sbjct 213 ATAATCGCCGGAGAAACTCGCTTCAAG 239
> AT3G10360.1 | Symbols: APUM4, PUM4 | pumilio 4 | chr3:3210884-3215556
REVERSE LENGTH=3692
Length=3692
Score = 486 bits (263), Expect = 3e-136
Identities = 362/403 (90%), Gaps = 33/403 (8%)
Strand=Plus/Minus
Query 1490 GTTCAAT-TAAAACTTTAGTA-CTTTTAAAAT-AAAAATATAGCAATTCTTGGGGAGCAA 1546
||||| | ||||||||||| | |||||||||| |||||||||||||||||||||||||||
Sbjct 3692 GTTCAGTGTAAAACTTTAG-AGCTTTTAAAATAAAAAATATAGCAATTCTTGGGGAGCAA 3634
Query 1547 AGCCGCAAGATATGT--A-C---A--T--AG--T-A-A--T-----TAAGCCATTGCTTT 1585
||||||||||||| | | | | | || | | | | ||||||||||||||
Sbjct 3633 AGCCGCAAGATATTTACATCGTAATGTGAAGAATAAGACCTAAAAGTAAGCCATTGCTTT 3574
Query 1586 ACATAAATAAAACAGCAAGGCTTCACCTTTTACACAACCAAAAGGAGATTTTTAATTTAA 1645
||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||
Sbjct 3573 ACATAAATAAAACAGCAAGGCTTCACCTTTTACACAACCAAAAGGAGATTTTTGATTTAA 3514
Query 1646 GCCAGTTGAGATCCAAAATACATTTATT-ACCGACTTAAATCTAAAACCCTCTATTTTAT 1704
|||||||| ||||||||||||||||||| || ||||||||||||||||||||||||||||
Sbjct 3513 GCCAGTTGGGATCCAAAATACATTTATTTACTGACTTAAATCTAAAACCCTCTATTTTAT 3454
Query 1705 TTACAAATTATTTGCAAAATATATATAACAGGAACCATCTTGAGATGCTTTGCTTAGTTA 1764
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3453 TTACAAATTATTTGCAAAATATATATAACAGGAACCATCTTGAGATGCTTTGCTTAGTTA 3394
Query 1765 GGCTGTACAAATAGCTCTCGGTCATCA----G--CCTCTTTCTCTGGTTGTGTTATAAAT 1818
||||||||||||||||||||||||||| | |||||| ||| ||||||||||||| |
Sbjct 3393 GGCTGTACAAATAGCTCTCGGTCATCAACTTGTACCTCTT-CTCAGGTTGTGTTATAAGT 3335
Query 1819 TAAGAAGCTTGAGGTTTTACTTACTCAGAGATGGTTAAGGAGT 1861
||||||||||||||||||||||||||||||||||||| |||||
Sbjct 3334 TAAGAAGCTTGAGGTTTTACTTACTCAGAGATGGTTATGGAGT 3292
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 92118000075
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5