BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg489029
Length=2706
Score E
Sequences producing significant alignments: (Bits) Value
AT5G21222.1 | Symbols: | protein kinase family protein | chr5:... 4176 0.0
AT5G21326.1 | Symbols: | Ca2+regulated serine-threonine protei... 1014 0.0
> AT5G21222.1 | Symbols: | protein kinase family protein | chr5:7208643-7213894
FORWARD LENGTH=3010
Length=3010
Score = 4176 bits (2261), Expect = 0.0
Identities = 2508/2629 (95%), Gaps = 9/2629 (0%)
Strand=Plus/Plus
Query 81 CCGAGACAATTCGCAGGTTGTGAAGAAGATCAAGAGGTGAAAATCTGAGTGTGATGAGTG 140
||| |||||||||||| |||||||||||||||||||||| || ||||| ||||||||||
Sbjct 271 CCGGGACAATTCGCAGCTTGTGAAGAAGATCAAGAGGTG-AACTCTGA--GTGATGAGTG 327
Query 141 AGCCAAAGGCCAGGCGTTGGGTTGGAAAATATGAGCTTGGAAGAATGATTGCCGAATGTT 200
||||||||| ||||||||||||||| ||||||||| |||||||| |||| | ||||||
Sbjct 328 AGCCAAAGGTCAGGCGTTGGGTTGGCAAATATGAGGTTGGAAGATTGATCGGTGAATGTA 387
Query 201 CTTTTGGGAAAGTGCGGAGTGCTGTTGATACTCAGACAGGAGACCCCGTGGCTCTGATGA 260
|||||||||| |||||||||||||||| || ||||||||||||| |||||| ||||||
Sbjct 388 ATTTTGGGAAACTGCGGAGTGCTGTTGACACAGAGACAGGAGACCCTGTGGCTTTGATGA 447
Query 261 TCCTTGATAAAGACAAGATTCTCAACCATAAGATGGCTGAACAAATAAAGAGAGAGATTT 320
||||||||||||||||| |||| || ||||||||||||||||||||||||||||| ||||
Sbjct 448 TCCTTGATAAAGACAAGGTTCTGAAGCATAAGATGGCTGAACAAATAAAGAGAGAAATTT 507
Query 321 CTACCATGAAGCTGATAAACCATCCTAATGTCGTGTGGCTGTATGAGGTTTTGGCGAGTA 380
||| ||||||||||| |||||||| ||||| ||| ||| ||||||||||||||||| |
Sbjct 508 CTATTATGAAGCTGATCAACCATCCAAATGTAGTGCAGCTTTATGAGGTTTTGGCGAGCA 567
Query 381 AGATGAAGATATATATTGTCCTAGAGTTCATCAGTGGTGGAGAGCTTTTTGAC-AGAATT 439
|| ||||||||||||||||||||||||||||||||||||| ||||||||||| || |||
Sbjct 568 AGGCGAAGATATATATTGTCCTAGAGTTCATCAGTGGTGGAAAGCTTTTTGACAAG-ATT 626
Query 440 AAAAATGATGGACGCATGAAAGAAGAGGAAGCACGGAGATATTTCCAGCAGCTTATCAAT 499
||||||||||| |||||||| ||||| ||||||| |||||||||||||||||||||||||
Sbjct 627 AAAAATGATGGGCGCATGAATGAAGACGAAGCACAGAGATATTTCCAGCAGCTTATCAAT 686
Query 500 GCAGTTGATTACTGCCACAGCAGGGGATTGTACCACAGAGACTTAAAGCCGGAAAATTTG 559
||||||||||||||||||||||||||| |||||||||||||||||||||| |||||||||
Sbjct 687 GCAGTTGATTACTGCCACAGCAGGGGAGTGTACCACAGAGACTTAAAGCCAGAAAATTTG 746
Query 560 CTTCTTGATGTGCAAGAGAATTTGAAAGTCTCTGACTTTGGATTGAGTGCCTTGTCCCGA 619
|||||||||| ||||||||||||||||||| |||| |||||||||| ||||||||||| |
Sbjct 747 CTTCTTGATGCGCAAGAGAATTTGAAAGTCGCTGAATTTGGATTGATTGCCTTGTCCCAA 806
Query 620 CAAGTCGGGGGTGATGGCCTTCATCACACTGCATGTGGAAATCCAAATTATGCTGCTCCT 679
|||| |||||| |||||||||| |||||||||||||||||||||| ||||||||||||||
Sbjct 807 CAAGCCGGGGGAGATGGCCTTCGTCACACTGCATGTGGAAATCCAGATTATGCTGCTCCT 866
Query 680 GAGGTTCTTAATGATCAAGGCTATGATGGGGCAAAGGCAGACTTGTGGTCCTGTGGAGTG 739
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 867 GAGGTTCTTAATGATCAAGGCTATGATGGGGCAAAGGCAGACTTGTGGTCCTGTGGAGTG 926
Query 740 ATACTCTTTGTACTACTTTCTGGGTATTTGCCTTTCGAGGATTCTAGTCTCACGACTCTG 799
|||||||||||||||||| ||||||||||||||||||||||||||||||||||||| |||
Sbjct 927 ATACTCTTTGTACTACTTGCTGGGTATTTGCCTTTCGAGGATTCTAGTCTCACGACGCTG 986
Query 800 TACAAAAAGATCTCATCTGCTGATTTCAGTTGTCCTCCTTGGCTCTCTTCTGGTGCTAAG 859
|||||||||||||||||||||||||| |||||||||||||||||||||||||||| ||||
Sbjct 987 TACAAAAAGATCTCATCTGCTGATTTTAGTTGTCCTCCTTGGCTCTCTTCTGGTGTTAAG 1046
Query 860 AATTTGATCGTCCGAATCTTGGATCCAAACCCTATGACTCGTATAACAATTCCAGAGATT 919
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1047 AATTTGATCGTCCGAATCTTGGATCCAAACCCTATGACTCGTATAACAATTCCAGAGATT 1106
Query 920 TTGGAAGATGTGTGGTTCAAGAAAGATTACAAGCCAGCTGTATTTGAAGAGAAGAAAGAA 979
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1107 TTGGAAGATGTGTGGTTCAAGAAAGATTACAAGCCAGCTGTATTTGAAGAGAAGAAAGAA 1166
Query 980 GCAAATTTGGCTGATGTGGAAGCTGTTTTCAAAGATTCTGAAGAAGGGAGAGTGCAACTG 1039
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1167 GCAAATTTGGCTGATGTGGAAGCTGTTTTCAAAGATTCTGAAGAAGGGAGAGTGCAACTG 1226
Query 1040 AGGTCATTTCCTTGTGTGATATGTTCAGGTGGTACTACTTGTGGGGATGTTCGTTCAAGG 1099
|||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||
Sbjct 1227 AGGTCATTTCCTTGTGTGATATGTTCAGGTGGTACTACTTGTGGTGATGTTCGTTCAAGG 1286
Query 1100 ACCAAACTGATGAACGGCTTGATAGAACGAGGGAGACCTCAAGAAGCTCATTCCATTTTC 1159
||||||||||||||||||||||||||| ||||||| ||||||||||||||||||||||||
Sbjct 1287 ACCAAACTGATGAACGGCTTGATAGAAAGAGGGAGGCCTCAAGAAGCTCATTCCATTTTC 1346
Query 1160 AACACACTTATTGAGGAAGGCCACAAGCCAAGCATCATCACATACACCACGCTTGTAACT 1219
||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||
Sbjct 1347 AACACACTTATTGAGGAAGGCCACAAGCCAAGCCTCATCACATACACCACGCTTGTAACT 1406
Query 1220 GCATTAACCCGTCAAAAGCACTTCCACTCTCTCCTCTCCCTCATCTCCAAAGTCGAGAAA 1279
||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1407 GCATTGACCCGTCAAAAGCACTTCCACTCTCTCCTCTCCCTCATCTCCAAAGTCGAGAAA 1466
Query 1280 AACGGCCTCAAACCCGACACCATTCTCTTCAACGCCATCATCAACGCCTCTTCCGAGTCT 1339
|||||||| ||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1467 AACGGCCTTAAACCTGACACCATTCTCTTCAACGCCATCATCAACGCCTCTTCCGAGTCT 1526
Query 1340 GGAAATCTCGATCAAGCAATGAAGATATTT-GAAAAAATGAAGGAAAGTGGATGTAAACC 1398
|||||||||||||||||||||||||| ||| |||||||||||||||||||||||||||||
Sbjct 1527 GGAAATCTCGATCAAGCAATGAAGAT-TTTCGAAAAAATGAAGGAAAGTGGATGTAAACC 1585
Query 1399 CACAGCAAGTACATTTAATACTCTCATCAAAGGATATGGTAAAATTGGTAAGCTAGAGGA 1458
||||||||||||||||||||||||||||||||||||||| |||||||||||||| |||||
Sbjct 1586 CACAGCAAGTACATTTAATACTCTCATCAAAGGATATGGCAAAATTGGTAAGCTGGAGGA 1645
Query 1459 ATCATCAAGACTCCTGGATATGATGTTACGGGATGAGATGCTCCAACCAAATGACAGAAC 1518
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1646 ATCATCAAGACTCCTGGATATGATGTTACGGGATGAGATGCTCCAACCAAATGACAGAAC 1705
Query 1519 ATGTAACATACTTGTCCAGGCTTGGTGTAACCAGAGGAAGATTGAAGAAGCATGGAACAT 1578
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1706 ATGTAACATACTTGTCCAGGCTTGGTGTAACCAGAGGAAGATTGAAGAAGCATGGAACAT 1765
Query 1579 TGTATATAAGATGCAATCTTTTGGGGTAAAACCTGATGCAGTCACTTTTAACACATTGGC 1638
|||||||||||||||||||| ||||||||||||||||| ||| || ||||||||||||||
Sbjct 1766 TGTATATAAGATGCAATCTTATGGGGTAAAACCTGATGTAGTTACGTTTAACACATTGGC 1825
Query 1639 AAGAGCGTATTCGCGGATCGGGTCAACATGTACAGCTGAGGATATGATCATACCAAGGAT 1698
|| ||||||| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1826 AAAAGCGTATGCGCGGATCGGGTCAACATGTACAGCTGAGGATATGATCATACCAAGGAT 1885
Query 1699 GTTACACAACAAAGTTAAACCGAATGTACGTACCTGTGGTACCATTGTAAATGGCTACTG 1758
||||||||| |||||||||||||||||||||||||| ||||||||||| |||||||||||
Sbjct 1886 GTTACACAATAAAGTTAAACCGAATGTACGTACCTGCGGTACCATTGTGAATGGCTACTG 1945
Query 1759 TGAAGAGGGAAAGATGGAAGAAGCTTTGAGGTTTTTCTATAGAATGAAGGAACTTGGAGT 1818
||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1946 TGAAGAGGGCAAGATGGAAGAAGCTTTGAGGTTTTTCTATAGAATGAAGGAACTTGGAGT 2005
Query 1819 TCATCCAAATCTGTTTGTTTTTAACTCTCTAATCAAAGGCTTTCTTAACATAAACGACAT 1878
|||||| ||||||||||||||||||||||||||||||||||| |||||||| ||||||||
Sbjct 2006 TCATCCTAATCTGTTTGTTTTTAACTCTCTAATCAAAGGCTTCCTTAACATCAACGACAT 2065
Query 1879 GGATGGAGTTGGTGAGGTGGTGGATCTGATGGAAGACTTCGGAGTAAAACCTGATGTGGT 1938
|||||||||||||||||||||||||||||||||||| || ||||||||||||||||||||
Sbjct 2066 GGATGGAGTTGGTGAGGTGGTGGATCTGATGGAAGAATTTGGAGTAAAACCTGATGTGGT 2125
Query 1939 AACGTTTAGCACTCTAATGAATGCATGGAGCTCTGTAGGAGATATGAAAAGATGCGAAGA 1998
|||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
Sbjct 2126 AACGTTTAGCACTCTAATGAATGCATGGAGTTCTGTAGGAGATATGAAAAGATGCGAAGA 2185
Query 1999 GATCTATAGAGATATGTTGGAAGGAGGAATAGATCCCGACATTCATGCATTTAGTATCCT 2058
|||||||| ||||||||||||||||||||||||||| || ||||||||||||||||||||
Sbjct 2186 GATCTATACAGATATGTTGGAAGGAGGAATAGATCCTGATATTCATGCATTTAGTATCCT 2245
Query 2059 TGCCAAAGGGTATGCTCGAGCTGGAGAGCCCGAAAAGGCGGAGCAAATCTTGAATCAGAT 2118
|||||||||||||||||||||||| ||||||||||| |||||||||||||||||||||||
Sbjct 2246 TGCCAAAGGGTATGCTCGAGCTGGGGAGCCCGAAAAAGCGGAGCAAATCTTGAATCAGAT 2305
Query 2119 GAGAAAATTCGGCGTTCGCCCAAATGTAGTGATCTACACACAGATCATAAGCGGTTGGTG 2178
||||||||| || |||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2306 GAGAAAATTTGGTGTTCGCCCAAATGTAGTGATCTACACACAGATCATAAGCGGTTGGTG 2365
Query 2179 CAGCGCAGGTGAAATGAAGAAAGCGATGCAGGTATATAACAAAATGCGTGGAAGCATTGG 2238
||| || |||||||||||||||||||||||||||||||| |||||| |||||| | ||||
Sbjct 2366 CAGTGCTGGTGAAATGAAGAAAGCGATGCAGGTATATAAGAAAATGTGTGGAATCGTTGG 2425
Query 2239 TTTATCGCCTAACTTGACTACTTACGAGACTCTTATATGGGGATTTGGGGAAGCTAAGCA 2298
|||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
Sbjct 2426 TTTATCGCCTAACTTGACTACTTACGAGACTCTTATATGGGGATTTGGGGAAGCGAAGCA 2485
Query 2299 GCCATGGAAAGCGGAAGAGCTTCTGAAAGAAATGGAAGAGAAGAATGTTGTTCCCACAAG 2358
|||||||||||| ||||||||||||||||| ||||||| |||||||||||||||||||||
Sbjct 2486 GCCATGGAAAGCAGAAGAGCTTCTGAAAGACATGGAAGGGAAGAATGTTGTTCCCACAAG 2545
Query 2359 GAAAACTATGCAACTCATAGCTGATGGATGGAAATCTATTGGTGTCTCTAATAGTAATGA 2418
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2546 GAAAACTATGCAACTCATAGCTGATGGATGGAAATCTATTGGTGTCTCTAATAGTAATGA 2605
Query 2419 TGCCAATACATTGGGATCTTCCTTCTCAACTTCCTCCAAACTCAACATCCCAAATAACAT 2478
||||||||||||||| |||||||||||||||||||| |||||||| ||||||||||| ||
Sbjct 2606 TGCCAATACATTGGGCTCTTCCTTCTCAACTTCCTCTAAACTCAATATCCCAAATAATAT 2665
Query 2479 CGCCTCGTCGAAAAGTCCATTGTT-TTCAAAAGGAATGCCTGAAAAACCAAAGCTATGTA 2537
||||||||||| |||||||||||| || ||||||||||||||||||||||||||||||||
Sbjct 2666 CGCCTCGTCGAGAAGTCCATTGTTCTT-AAAAGGAATGCCTGAAAAACCAAAGCTATGTA 2724
Query 2538 TCAAGTCTCAGTTTGGTTTGAGGCAGAGTGTTTTGGTGGTGTTGTGCAGAGACCAGATTG 2597
||||||||||||||||||||||||||| | ||||||||||||||||||||||||||||||
Sbjct 2725 TCAAGTCTCAGTTTGGTTTGAGGCAGACTCTTTTGGTGGTGTTGTGCAGAGACCAGATTG 2784
Query 2598 GGGAAGCTGGGAGATTTTGCAAGTTCATGTAGACTTTGTTTTGCTTTATAGATGGAGCTA 2657
||||||| ||||||||||| ||||||||||||| ||||||||||| || |||||||||||
Sbjct 2785 GGGAAGCCGGGAGATTTTGTAAGTTCATGTAGAATTTGTTTTGCTGTAGAGATGGAGCTA 2844
Query 2658 AATTTAAGGGTAAGAAAGGCTAACCAAAGATTGGTGTCGATCGTTTTTT 2706
|||| |||||||||||| ||| | ||||||||||||||||||| |||||
Sbjct 2845 AATTAAAGGGTAAGAAAAGCTGATCAAAGATTGGTGTCGATCGATTTTT 2893
> AT5G21326.1 | Symbols: | Ca2+regulated serine-threonine protein
kinase family protein | chr5:7217340-7222007 FORWARD LENGTH=1784
Length=1784
Score = 1014 bits (549), Expect = 0.0
Identities = 808/931 (87%), Gaps = 25/931 (3%)
Strand=Plus/Plus
Query 105 GAAGATCAAGAGGTGAA-AATCTGAGTGTGATGAGT-GAGCCAAAGG-CCAGGCGTTGGG 161
||||||||||||||||| | || | | ||||||| | | |||||||| || |||| | |
Sbjct 173 GAAGATCAAGAGGTGAACATTC-G-GAGTGATGAATCG-GCCAAAGGTTCA-GCGTCGTG 228
Query 162 TTGGAAAATATGAGCTTGG-AAGAATGATT-GCCGAATGTTCTTTTGGGAAAGTGCGGAG 219
||||||| |||||| |||| ||| | | || | | || | |||||| |||||| ||| |
Sbjct 229 TTGGAAAGTATGAGGTTGGAAAG-ACGCTTGGAC-AAGGCACTTTTGCGAAAGTTCGGTG 286
Query 220 TGCTGTTGATACTCAGACAGGAGACCCCGTGGCTCTGATGATCCTTGATAAAGACAAGAT 279
||||||| ||||| |||||||||| | ||||||||| | || |||||||| || ||| |
Sbjct 287 TGCTGTTAATACTGAGACAGGAGAACGCGTGGCTCTCAAAATACTTGATAAGGAGAAGGT 346
Query 280 TCTCAACCATAAGATGGCTGAACAAATAAAGAGAGAGATTTCTACCATGAAGCTGATAAA 339
||| || ||||||||||||||||| |||| |||||| |||| ||| ||||||||||||||
Sbjct 347 TCTTAAGCATAAGATGGCTGAACAGATAAGGAGAGAAATTTGTACTATGAAGCTGATAAA 406
Query 340 CCATCCTAATGTCGTGTGGCTGTATGAGGTTTTGGCGAGTAAGA-TGAAGATATATATTG 398
|||||| ||||| ||| |||||||||||||||||||||| |||| | |||||||||||||
Sbjct 407 CCATCCAAATGTAGTGCGGCTGTATGAGGTTTTGGCGAGCAAGACT-AAGATATATATTG 465
Query 399 TCCTAGAGTTCATCAGTGGTGGAGAGCTTTTTGACAGAATTAAAAATGATGGACGCATGA 458
| ||||||||| || |||||||||||||||||||| |||| | ||||||| ||| |||
Sbjct 466 TTCTAGAGTTCGGCACTGGTGGAGAGCTTTTTGACAAAATTGTACATGATGGGCGCTTGA 525
Query 459 AAGAAGAGGAA-GCACGGAGATATTTCCAGCAGCTTATCAATGCAGTTGATTACTGCCAC 517
||||||| ||| ||||||| |||||| ||||||||||| ||||||||||||||||||||
Sbjct 526 AAGAAGA-GAATGCACGGAAATATTTTCAGCAGCTTATTAATGCAGTTGATTACTGCCAT 584
Query 518 AGCAGGGGATTGTACCACAGAGACTTAAAGCCGGAAAATTTGCTTCTTGATG-TGCAA-G 575
||||||||| |||||||| | ||||||||||||||||| |||||||| |||| | ||| |
Sbjct 585 AGCAGGGGAGTGTACCACCGGGACTTAAAGCCGGAAAACTTGCTTCTAGATGCT-CAAGG 643
Query 576 AGAATTTGAAAGTCTCTGACTTTGGATTGAGTGCCTTGTCCCGACAAGTCGGGGGTGATG 635
| ||| ||||||||||||| |||||||||||||| ||||||||||||||| |||||||||
Sbjct 644 A-AATCTGAAAGTCTCTGATTTTGGATTGAGTGCGTTGTCCCGACAAGTCAGGGGTGATG 702
Query 636 GCCTTCATCACACTGCATGTGGAAATCCAAATTATGCTGCTCCTGAGGTTCTTAATGATC 695
| |||| |||||||||||| |||| |||||| ||||||||||||||||||||||||||||
Sbjct 703 GTCTTCTTCACACTGCATGCGGAACTCCAAACTATGCTGCTCCTGAGGTTCTTAATGATC 762
Query 696 AAGGCTATGATGGGGCAAAGGCAGACTTGTGGTCCTGTGGAGTGATACTCTTTGTACTAC 755
|||| ||||| ||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 763 AAGGTTATGACGGGGCAACCGCAGACTTGTGGTCCTGTGGAGTGATACTCTTTGTACTAC 822
Query 756 TTTCTGGGTATTTGCCTTTCGAGGATTCTAGTCTCACGACTCTGTACAAAAAGATC-TCA 814
|| |||| || ||||||||||||||||||| ||||| ||| ||||| |||||||| | |
Sbjct 823 TTGCTGGCTACTTGCCTTTCGAGGATTCTAATCTCATGACGCTGTATAAAAAGATAAT-A 881
Query 815 TCTGCTGATT-TCAGTTGTCCTCCTTGGCTCTCTTCTGGTGCTAAGAATTTGATCGTCCG 873
||| ||| | ||| ||||||||||||||||||| ||||||||||||| ||||| |||||
Sbjct 882 GCTGGTGAATATCA-TTGTCCTCCTTGGCTCTCTCCTGGTGCTAAGAACTTGATTGTCCG 940
Query 874 AATCTTGGATCCAAACCCTATGACTCGTATAACAATTCCAGAGATTTTGGAAGATGTGTG 933
|||||||||||||||||||||||||||||| |||||||||||| || ||| ||||| |||
Sbjct 941 AATCTTGGATCCAAACCCTATGACTCGTATCACAATTCCAGAGGTTCTGGGAGATGCGTG 1000
Query 934 GTTCAAGAAAGATTACAAGCCAGCTGTATTT-GAAGAGAAGAAAGAAGCAAATTTGGCTG 992
|||||||||| |||||||||||||||| ||| || |||||| ||||||| || |||| ||
Sbjct 1001 GTTCAAGAAAAATTACAAGCCAGCTGT-TTTCGAGGAGAAGGAAGAAGCCAACTTGGATG 1059
Query 993 ATGTGGAAGCTGTTTTCAAAGATTCTGAAGA 1023
||||||| ||||||||||||||||| |||||
Sbjct 1060 ATGTGGACGCTGTTTTCAAAGATTCAGAAGA 1090
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 134397240297
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5