BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg489848
Length=1335
Score E
Sequences producing significant alignments: (Bits) Value
AT4G10030.1 | Symbols: | alpha/beta-Hydrolases superfamily pro... 1288 0.0
AT4G10020.1 | Symbols: AtHSD5, HSD5 | hydroxysteroid dehydrogen... 261 1e-68
> AT4G10030.1 | Symbols: | alpha/beta-Hydrolases superfamily protein
| chr4:6270241-6273241 REVERSE LENGTH=1535
Length=1535
Score = 1288 bits (697), Expect = 0.0
Identities = 844/912 (93%), Gaps = 21/912 (2%)
Strand=Plus/Plus
Query 1 GTAATGTCGGCGGTATCTTGCTCCACGGCGAGCTCTAGCGGCGGATGTGGAATTAGGTTT 60
|||||||||||||| || |||||||||||||| |||||||| ||||||| | ||||||||
Sbjct 141 GTAATGTCGGCGGTCTCGTGCTCCACGGCGAGTTCTAGCGGAGGATGTGCATTTAGGTTT 200
Query 61 CAATCAAAGACATTTGCTTCATCAGCAAAAACCCATTCTCCTAATCTCATCAGAATCGGG 120
| ||||||| | ||| | || ||||||||||||| |||||||||| |||
Sbjct 201 CGATCAAAG--A-------CAT---C---AATCCATTCTCCTAATTTCATCAGAATTGGG 245
Query 121 CCTAATTCTCAGAGTGACAGATTCTGT-ATGCATCGTTTGGAATTGGTACATGGAACTCC 179
| |||||||||||||||||||||||| || ||||||||||||||||||||||||||| |
Sbjct 246 ACAAATTCTCAGAGTGACAGATTCTGTAAT-CATCGTTTGGAATTGGTACATGGAACT-C 303
Query 180 G-AAAGTCAGAGGAACATCTATACGTATGGCATTGGTTGATGAGAGACAATCAACGAGTA 238
| ||||| | |||| ||||| ||||||||||||| ||||| ||||||||||||||||| |
Sbjct 304 GTAAAGTGAAAGGAGCATCTGTACGTATGGCATTAGTTGAGGAGAGACAATCAACGAGCA 363
Query 239 AAGATTTTGCTGAGCCTCCTCGGATTCTGGCGTATGATCTTGTTCAAGGGGCACTTGTGA 298
||||| ||||||||||||||||| | ||||||||||||||||||||||| ||||||||||
Sbjct 364 AAGATGTTGCTGAGCCTCCTCGGGTCCTGGCGTATGATCTTGTTCAAGGAGCACTTGTGA 423
Query 299 AATGGAGATGGAAGGATGACAAATCTGTTCCAGATACACCTACCGCTGTTCTCTTACACG 358
||||||||||||||||||||||||||||||||||||||||||| ||||||||| ||||||
Sbjct 424 AATGGAGATGGAAGGATGACAAATCTGTTCCAGATACACCTACTGCTGTTCTCCTACACG 483
Query 359 GAATTCTAGGGAGCGGGAAAAACTGGGGCACTTTTGCCCGAAGATTGGCTCATGAGTTCC 418
||||| ||||||||||||||||||||||||||||||| || |||||||||||||| ||||
Sbjct 484 GAATTTTAGGGAGCGGGAAAAACTGGGGCACTTTTGCTCGTAGATTGGCTCATGAATTCC 543
Query 419 CAACTTGGCAGTTTCTCTTGGTAGATCTTCGTTGCCATGGGGATTCAGCATCTCTCAAGA 478
|||| ||||||||||| |||||||| || |||||||||||||||||| ||||||||||||
Sbjct 544 CAACGTGGCAGTTTCTATTGGTAGACCTGCGTTGCCATGGGGATTCAACATCTCTCAAGA 603
Query 479 AGAGAGGTCCACACTCTGTTGCTACAACAGCTTCTGATGTTCTAAAACTTGTTGGTCAGT 538
|||||||||||||||| |||||||| || |||||||||||||||||||||||||||||||
Sbjct 604 AGAGAGGTCCACACTCGGTTGCTACTACTGCTTCTGATGTTCTAAAACTTGTTGGTCAGT 663
Query 539 TGAGGTTAACACCTCGGGTCCTTGTTGGTCATAGCTTTGGGGGGAAAGTTGTATTAAGCA 598
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 664 TGAGGTTAACACCTCGGGTCCTTGTTGGTCATAGCTTTGGGGGGAAAGTTGTATTAAGCA 723
Query 599 TGGTGGAACAAGCAGCTAAGCCTCTTCCAAGACCAGTCCGGGCTTGGGTATTGGATGCTA 658
|||| || ||||||||||||||||||||| |||||||||| |||||||||||||||||||
Sbjct 724 TGGTAGAGCAAGCAGCTAAGCCTCTTCCACGACCAGTCCGAGCTTGGGTATTGGATGCTA 783
Query 659 CTCCTGGAAAAGTTCGTGCAGGAGGAGATGGAGAAGATCATCCACGAGAGCTTATATCAT 718
||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 784 CTCCTGGTAAAGTTCGTGCAGGAGGAGATGGAGAAGATCATCCACGAGAGCTTATATCAT 843
Query 719 TTCTACGTAAATTGCCAAAAGTGGTCTTGTCAAAGCGCGAGGTTCTTAAAGCTCTTATAA 778
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 844 TTCTACGTAAATTGCCAAAAGTGGTCTTGTCAAAGCGCGAGGTTCTTAAAGCTCTTATAA 903
Query 779 ATGAAGGGTTTTCCAATGATGTCGCGCAGTGGGTCATTACCAATCTCAAACCTACTGGAC 838
||||||||||||||||||||||||| |||||||||||||||||||||| |||||||||||
Sbjct 904 ATGAAGGGTTTTCCAATGATGTCGCACAGTGGGTCATTACCAATCTCAGACCTACTGGAC 963
Query 839 CATCGGCTTCTAGCTTCTCGTGGACATTTGACCTAGACGGGATCGCTGAACTTTACCAAA 898
|||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 964 CATCAGCTTCTAGCTTCTCGTGGACATTTGACCTAGACGGGATCGCTGAACTTTACCAG- 1022
Query 899 TCTTCC-AAGAG 909
|||| | |||||
Sbjct 1023 TCTTACGAAGAG 1034
Score = 545 bits (295), Expect = 3e-154
Identities = 421/475 (89%), Gaps = 36/475 (8%)
Strand=Plus/Plus
Query 897 AATCTTCCAAGA-G-GTACATGTGAATTTTCTGAAAGCGGAAAGAAGCTTGCACCGTTGG 954
|||||||||||| | |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1059 AATCTTCCAAGAGGAGTACATGTGAATTTTCTGAAAGCGGAAAGAAGCTTGCACCGTTGG 1118
Query 955 GCCCTAAAAGACCTTCAGCGAATCCACGCCGCTGAAGAGCTAGCCTCTGAAGAAGGCGGT 1014
|||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1119 GCCCTAGAAGACCTTCAGCGAATCCACGCCGCTGAAGAGCTAGCCTCTGAAGAAGGCGGT 1178
Query 1015 GGCGTAGAAATGCATGTCCTGGAAGATGCCGGTCATTGGGTTCACACAGATAACCCGGAT 1074
|| ||||||||||||||| |||||||||||||||||||||||||||| ||||||||||||
Sbjct 1179 GGTGTAGAAATGCATGTCTTGGAAGATGCCGGTCATTGGGTTCACACGGATAACCCGGAT 1238
Query 1075 GGTCTCTTCAGGATCTTGTCATCTTCTTTCCAGGTTCTCAGAGCCTAGTGGTGCTGAACG 1134
|||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1239 GGTCTCTTCAGGATCTTGTCATCTTCTTTCCAGGTTCTCAGAGCCTAGAGGTGCTGAACG 1298
Query 1135 --Ctt--tt----t-ttGTT-T----AATACTTGTG-ACAT-TT-G----A----CAGAT 1169
||| || | || || | |||||||||| | | || | | ||| |
Sbjct 1299 GTCTTGGTTCGGCTCTTTTTCTGTGTAATACTTGTGGAGAAGTTAGGTCTAAAAACAGTT 1358
Query 1170 G-CA-ATA---T--CATATACATCAACATTGTGTCCGTGTAATGTTTTGTGTGACATAAG 1222
| || ||| | |||||||||||||| ||||||||||||||||| |||||||||||||
Sbjct 1359 GACAGATACAATATCATATACATCAACACTGTGTCCGTGTAATGTTCTGTGTGACATAAG 1418
Query 1223 TAATTAAGAAGTAGCATCATATGGCTGTATCATCCGGCATTATTCTTTACTCGATGTCCT 1282
||||| ||||||| ||||||||||||||||||||||| ||||||||| ||||||||||||
Sbjct 1419 TAATTCAGAAGTAACATCATATGGCTGTATCATCCGGTATTATTCTTGACTCGATGTCCT 1478
Query 1283 TTACATTGCTTTTCTTTTT--GTCGTTCACTTAGTCGGAGAAAGTTCTAAAGCTC 1335
||||||||||||||||||| || |||||||| ||| ||||||||||||||||||
Sbjct 1479 TTACATTGCTTTTCTTTTTTTGTGGTTCACTTGGTCAGAGAAAGTTCTAAAGCTC 1533
> AT4G10020.1 | Symbols: AtHSD5, HSD5 | hydroxysteroid dehydrogenase
5 | chr4:6268289-6270434 FORWARD LENGTH=1499
Length=1499
Score = 261 bits (141), Expect = 1e-68
Identities = 168/181 (93%), Gaps = 2/181 (1%)
Strand=Plus/Minus
Query 1157 ACATTTGACAGATGCAATATCATATACATCAACATTGTGTCCGTGTAATGTTTTGTGTGA 1216
||| ||||||||| |||||||||||||||||||| ||||||||||||||||| |||||||
Sbjct 1488 ACAGTTGACAGATACAATATCATATACATCAACACTGTGTCCGTGTAATGTTCTGTGTGA 1429
Query 1217 CATAAGTAATTAAGAAGTAGCATCATATGGCTGTATCATCCGGCATTATTCTTTACTCGA 1276
||||||||||| ||||||| ||||||||||||||||||||||| ||||||||| ||||||
Sbjct 1428 CATAAGTAATTCAGAAGTAACATCATATGGCTGTATCATCCGGTATTATTCTTGACTCGA 1369
Query 1277 TGTCCTTTACATTGCTTTTCTTTTT--GTCGTTCACTTAGTCGGAGAAAGTTCTAAAGCT 1334
||||||||||||||||||||||||| || |||||||| ||| |||||||||||||||||
Sbjct 1368 TGTCCTTTACATTGCTTTTCTTTTTTTGTGGTTCACTTGGTCAGAGAAAGTTCTAAAGCT 1309
Query 1335 C 1335
|
Sbjct 1308 C 1308
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 65712513405
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5