BLASTN 2.2.26+ Reference: Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000), "A greedy algorithm for aligning DNA sequences", J Comput Biol 2000; 7(1-2):203-14. Database: halleri_transcript_ahg.fa 32,672 sequences; 38,939,717 total letters Query= AT1G23840.1 Length=1080 Score E Sequences producing significant alignments: (Bits) Value Ahg921440 jgi|Araly1|921440|scaffold_102721.1 547 5e-155 Ahg472644 jgi|Araly1|472644|fgenesh2_kg.1__2598__AT1G23830.1 252 5e-66 Ahg921441 jgi|Araly1|921441|scaffold_102722.1 207 1e-52 > Ahg921440 jgi|Araly1|921440|scaffold_102721.1 Length=731 Score = 547 bits (296), Expect = 5e-155 Identities = 352/378 (93%), Gaps = 8/378 (2%) Strand=Plus/Plus Query 701 ACATTAGTATGGTTGTTTCGGTCTTAGAAGAGGAAGAAGATAGTAAAGGCATCTATGGAA 760 |||||||||||||||||||||||||||| |||||||||||| |||||||||||||||||| Sbjct 349 ACATTAGTATGGTTGTTTCGGTCTTAGAGGAGGAAGAAGATGGTAAAGGCATCTATGGAA 408 Query 761 GTAGTGCTCTGTCTCTTTCAGCTTGGTACTTAAGAGGACAAGAGAAGCGTGACTTCTGGA 820 | |||||| |||||||||| ||||||||||||||||||||||||||||||||| |||||| Sbjct 409 GCAGTGCTTTGTCTCTTTCCGCTTGGTACTTAAGAGGACAAGAGAAGCGTGACCTCTGGA 468 Query 821 TGATGCTAG-TGTTCTTGGTCGGCGCTCTTGTGACAAGGATGCCATGTTTATACTACAAA 879 ||||||| | ||||||||||||||||||||||||| |||||||| ||||||||||||||| Sbjct 469 TGATGCT-GATGTTCTTGGTCGGCGCTCTTGTGACGAGGATGCCGTGTTTATACTACAAA 527 Query 880 TGCAGTGAAAGTCTGAGTGGGAATGGAGTGTTGTACACTGGTCTCTATGTAAGTTTGATT 939 ||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||| Sbjct 528 TGCAGTGAAAGTCTGAGTGGGAATGGAGTGTTGTACACTGGTCTCTATGTAGGTTTGATT 587 Query 940 TGTGTTGGGAATGTCGTTAAATGGGTTTCTTGTGTTGTTTGGTACCATGATTGTAACACA 999 |||||||||||||| ||||||||||| |||||||||||||| |||||||||||||||||| Sbjct 588 TGTGTTGGGAATGTGGTTAAATGGGTATCTTGTGTTGTTTGTTACCATGATTGTAACACA 647 Query 1000 AGGGTCTTGAGGAAAAAGGGTGACGTGGAGATTGGGTCAAAAGC------TTTTGCTACA 1053 | ||| |||||||| |||||||| || |||||||| |||||||| |||||||||| Sbjct 648 AAGGTTTTGAGGAAGAAGGGTGATGTAGAGATTGGATCAAAAGCCAAGGCTTTTGCTACA 707 Query 1054 TAGAGAAATTGTTTGTGA 1071 |||||||||||||||||| Sbjct 708 TAGAGAAATTGTTTGTGA 725 Score = 481 bits (260), Expect = 5e-135 Identities = 320/349 (92%), Gaps = 4/349 (1%) Strand=Plus/Plus Query 24 AGAAAGAGATCAACAAATGGAGACAAAGAGCGAGGAGAAGCTAAGTGTCATCGAACTACT 83 |||||||||| ||||||||||||| |||||||| | |||||||||| ||||||||||||| Sbjct 5 AGAAAGAGATAAACAAATGGAGACGAAGAGCGACGTGAAGCTAAGTATCATCGAACTACT 64 Query 84 AAAACGAGCT-CTTAAGCTACTCTTTGGTAACATCAATCTCGCTCTCTTCCTCTTTCTT- 141 |||||||||| | |||||||||||||||||||||||||||||||||||| || ||||| Sbjct 65 AAAACGAGCTGC-GAAGCTACTCTTTGGTAACATCAATCTCGCTCTCTTCGTC-TTCTTC 122 Query 142 TGCTCTCTCCCACTGTTTTGTTTCTTGATCTTCTTCGAGCTCTCCCTCCAAACCACTGTC 201 ||||| |||||||||||||||||||||||||||||||||||||| |||||||| |||||| Sbjct 123 TGCTCCCTCCCACTGTTTTGTTTCTTGATCTTCTTCGAGCTCTCTCTCCAAACTACTGTC 182 Query 202 TCACTCGCTTCCACATACATCTCCAAACTAGTCAATTCTGAGGAGGACTTGTCGGAGAAT 261 |||||||||||||||||| ||||||||||||||||||||||||| ||||| ||||||||| Sbjct 183 TCACTCGCTTCCACATACCTCTCCAAACTAGTCAATTCTGAGGAAGACTTATCGGAGAAT 242 Query 262 GATCTTCTTCCTTGGCTGATTCAGACAACTCTGCTCTACTTCTTTCCCTATACTATTCTT 321 |||||| |||| ||| || | |||||| |||| ||||||||||||||||| |||||| | Sbjct 243 GATCTTATTCCATGGTTGGTGCAGACATCTCTACTCTACTTCTTTCCCTACACTATTATC 302 Query 322 GACCTCCTTACCACCACCACGATCGTTGCAGCATCATCGATTGCTTACA 370 ||||||||||||||||||||||||||||||||||| |||||| |||||| Sbjct 303 GACCTCCTTACCACCACCACGATCGTTGCAGCATCTTCGATTTCTTACA 351 > Ahg472644 jgi|Araly1|472644|fgenesh2_kg.1__2598__AT1G23830.1 Length=1091 Score = 252 bits (136), Expect = 5e-66 Identities = 211/246 (86%), Gaps = 10/246 (4%) Strand=Plus/Plus Query 26 AAAGAGATCAACAAATGGAGACAAAGAGCGAGGAGAAGCTAAGTGTCATCGAACTACTAA 85 ||||||||||||| |||||||| |||||||| ||||||||||||||||||||||| |||| Sbjct 1 AAAGAGATCAACAGATGGAGACCAAGAGCGAAGAGAAGCTAAGTGTCATCGAACTTCTAA 60 Query 86 AACGAGCT-CTTAAGCTACTCTTTGGTAACATCAATCTCGCT-CTCTTCCTCTTTCTTTG 143 ||| |||| | ||||||||||| ||||| | ||||||| | |||||||| | || || Sbjct 61 AACTAGCTGC-GAAGCTACTCTTCGGTAATAGCAATCTC-TTGCTCTTCCTATGCCTCTG 118 Query 144 CTCTCTCCCACTGTTTTGTTTCTTGATCTTCTTCGAGCTCTCCCTCCAAACCACTGTCTC 203 ||||||||||||||||||||||||||||||||| |||||||| ||||||||||||||||| Sbjct 119 CTCTCTCCCACTGTTTTGTTTCTTGATCTTCTTTGAGCTCTCACTCCAAACCACTGTCTC 178 Query 204 ACTCGCTTCCACA-TACATCTCCAAACTAGTCAATTCT-G-AGGAGGACTTGTCGGAGAA 260 ||| ||||| || | | ||| ||||||| ||| | || | || |||||||| | ||||| Sbjct 179 TCTCACTTCC-CAATTCCTCTTCAAACTACTCACT-CTTGGAG-AGGACTTGCCAGAGAA 235 Query 261 TGATCT 266 |||||| Sbjct 236 TGATCT 241 > Ahg921441 jgi|Araly1|921441|scaffold_102722.1 Length=1108 Score = 207 bits (112), Expect = 1e-52 Identities = 383/506 (76%), Gaps = 50/506 (10%) Strand=Plus/Plus Query 38 AAATGGAGACAAAGAGCGAGGAGAAGCTAAGTGT-CATCGAAC-TACTAAAACGAGC-TC 94 ||| ||||| |||| ||||||| | ||| || | |||| ||| |||| |||||||| | Sbjct 14 AAA-GGAGATGAAGATCGAGGAGGATCTAGGT-TACATC-AACGTACTCAAACGAGCAAC 70 Query 95 TTAAGCTACTCTTTGGTAACATCAATCTCGCTCTCTTCCTCTTTCTTTGCTCTCTCCCAC 154 ||||| || | |||||||||||||||| ||||||||||| |||||||||||||| | Sbjct 71 -AAAGCTTCTATGGGGTAACATCAATCTCGTTCTCTTCCTCTGCCTTTGCTCTCTCCCTC 129 Query 155 TGTTTTGTTTCTTGATCTTCTTCGAGCTCTCCCTCCAAACCACTGTCTCACTCGCTTC-C 213 | ||||||||||||||||||||||||||||| |||||||||||||||||||||||||| | Sbjct 130 TCTTTTGTTTCTTGATCTTCTTCGAGCTCTCACTCCAAACCACTGTCTCACTCGCTTCTC 189 Query 214 ACATACATC-TCC-A-AACT-A------G---T--C-A--A--TTC-T-GAGGAG--GAC 249 | |||| || | | | |||| | | | | | | ||| | ||| | | | Sbjct 190 A-ATACCTCGTTCGACAACTCAATAATTGGAATTACTACTACGTTCCTCGAGTTGCTGCC 248 Query 250 TTGTCGGAGAATGATCTTCTTCC-TTGGCTGATTCAGACAACTCTGCTCTACTTCTTTCC 308 |||||||||||| || ||||| ||| || || |||||| ||| |||||||| || || Sbjct 249 TTGTCGGAGAAT---CTGCTTCCATTG-CTTATCCAGACATTTCTACTCTACTTGTT-CC 303 Query 309 C-TATA-CTATTCTTGACCTCCTTACCACCACCACGATCGT-TGCAGCATCATCGATTGC 365 | || | | |||| |||||||| |||||||||| || || | | ||||| | ||||| Sbjct 304 CGTACAGCC-TTCTAGACCTCCTCACCACCACCATAATGGTCTCC-GCATCTTGGATTG- 360 Query 366 TT-ACACATCTGAGGAAGAGCCGTTAGGGTT-GCTTTATTTGGTAGGGAGATCTTTCA-A 422 || |||| || | ||| || |||||| |||| | | ||| ||| ||||| | | | | Sbjct 361 TTCACACGTCCGGGGAGGAACCGTTAAGGTTCGGTCAATT-GGTTCGGAGAACCGT-AGA 418 Query 423 GCTATGTCAGAACAAAGTAGGAGGTTGTCTGATCACATCTCTCTATGTCCTTCTCTTGTC 482 | ||||||| |||| | | | ||||||||||||||| ||||| || ||||||| |||| Sbjct 419 GATATGTCAAAACAGACTTGAAGGTTGTCTGATCACCTCTCTGTACATCCTTCTGATGTC 478 Query 483 GACCTCTGTTTTCCTCGGTTTGTTCT 508 ||| ||||| || | ||||| |||| Sbjct 479 AACCCCTGTTCTCTTTGGTTTCTTCT 504 Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 40219677435 Database: halleri_transcript_ahg.fa Posted date: Sep 25, 2014 2:02 AM Number of letters in database: 38,939,717 Number of sequences in database: 32,672 Matrix: blastn matrix 1 -2 Gap Penalties: Existence: 0, Extension: 2.5