BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: halleri_transcript_ahg.fa
32,672 sequences; 38,939,717 total letters
Query= AT1G66410.2
Length=850
Score E
Sequences producing significant alignments: (Bits) Value
Ahg925800 jgi|Araly1|925800|scaffold_201361.1 651 0.0
Ahg947442 jgi|Araly1|947442|scaffold_703671.1 472 3e-132
Ahg489030 jgi|Araly1|489030|fgenesh2_kg.6__2164__AT5G21274.1 363 2e-99
> Ahg925800 jgi|Araly1|925800|scaffold_201361.1
Length=490
Score = 651 bits (352), Expect = 0.0
Identities = 384/399 (96%), Gaps = 3/399 (1%)
Strand=Plus/Plus
Query 304 TGGTTGCATCACCACAAAGGAGCTGGGAACAGTGATGCGGTCACTAGGGCAGAACCCAAC 363
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 95 TGGTTGCATCACCACAAAGGAGCTGGGAACAGTGATGCGGTCACTAGGGCAGAACCCAAC 154
Query 364 AGAAGCTGAGCTACAAGACATGATCAACGAGGTTGACGCTGATGGAAACGGAACCATTGA 423
|||||||||||||||||||||||||||||||||||| |||||||||||||| ||||||||
Sbjct 155 AGAAGCTGAGCTACAAGACATGATCAACGAGGTTGATGCTGATGGAAACGGCACCATTGA 214
Query 424 CTTCCCTGAGTTCTTAAACCTAATGGCTAAAAAGATGAAGGACACAGACTCGGAGGAAGA 483
||||||||||||| ||||||| |||||||| |||||||||||||||||||||||||||||
Sbjct 215 CTTCCCTGAGTTCCTAAACCTTATGGCTAAGAAGATGAAGGACACAGACTCGGAGGAAGA 274
Query 484 GCTGAAAGAAGCTTTCAGGGTGTTCGACAAAGACCAGAACGGTTTCATATCCGCTGCAGA 543
|||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
Sbjct 275 GCTGAAAGAAGCTTTCAGGGTGTTCGACAAAGACCAGAACGGGTTCATATCCGCTGCAGA 334
Query 544 GCTTCGCCATGTGATGACGAATCTAGGTGAGAAACTGACTGATGAAGAGGTCGAGGAGAT 603
||| |||||||||||||| ||| |||||||||||||||||||||||||||||||||||||
Sbjct 335 GCTGCGCCATGTGATGACTAATTTAGGTGAGAAACTGACTGATGAAGAGGTCGAGGAGAT 394
Query 604 GATTCGTGAAGCAGATGTTGATGGAGATGGTCAGATAAACTACGAGGAGTTTGTCAAGAT 663
||| |||||||| |||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 395 GATCCGTGAAGCTGATGTTGATGGAGATGGTCAGATAAACTACGAGGAGTTTGTCAAGAT 454
Query 664 TATGATGGCTAAGTGATGATGATAAGaaaaaaaTGAAGA 702
|||||||||||||||||||| | | ||| ||||||||||
Sbjct 455 TATGATGGCTAAGTGATGAT-A-A-GAAGAAAATGAAGA 490
Score = 165 bits (89), Expect = 5e-40
Identities = 89/89 (100%), Gaps = 0/89 (0%)
Strand=Plus/Plus
Query 187 AGAAGAAGAAGCTATGGCGGATCAGCTAACTGATGAACAGATCTCTGAGTTTAAGGAAGC 246
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 8 AGAAGAAGAAGCTATGGCGGATCAGCTAACTGATGAACAGATCTCTGAGTTTAAGGAAGC 67
Query 247 TTTTAGCCTCTTCGACAAAGATGGAGATG 275
|||||||||||||||||||||||||||||
Sbjct 68 TTTTAGCCTCTTCGACAAAGATGGAGATG 96
> Ahg947442 jgi|Araly1|947442|scaffold_703671.1
Length=495
Score = 472 bits (255), Expect = 3e-132
Identities = 343/386 (89%), Gaps = 3/386 (1%)
Strand=Plus/Plus
Query 304 TGGTTGCATCACCA-CAAAGGAGCTGGGAACAGTGATGCGGTCACTAGGGCAGAACCCAA 362
|||||||||||| | |||| |||||||||||||| ||||| |||||||| || |||||||
Sbjct 100 TGGTTGCATCACAACCAAA-GAGCTGGGAACAGTTATGCGTTCACTAGGACAAAACCCAA 158
Query 363 CAGAAGCTGAGCTACAAGACATGATCAACGAGGTTGACGCTGATGGAAACGGAACCATTG 422
|||| |||||||| ||||||||||||||||||||||| || ||||||||||| || ||||
Sbjct 159 CAGAGGCTGAGCTTCAAGACATGATCAACGAGGTTGATGCAGATGGAAACGGCACAATTG 218
Query 423 ACTTCCCTGAGTTCTTAAACCTAATGGCTAAAAAGATGAAGGACACAGACTCGGAGGAAG 482
| ||||| |||||| | ||||| |||||||| |||||||| ||||| ||||| |||||||
Sbjct 219 ATTTCCCAGAGTTCCTGAACCTGATGGCTAAGAAGATGAAAGACACTGACTCAGAGGAAG 278
Query 483 AGCTGAAAGAAGCTTTCAGGGTGTTCGACAAAGACCAGAACGGTTTCATATCCGCTGCAG 542
|||| |||||||| |||||||| |||||||||||||||||||||||||| |||||||| |
Sbjct 279 AGCTAAAAGAAGCCTTCAGGGTTTTCGACAAAGACCAGAACGGTTTCATCTCCGCTGCTG 338
Query 543 AGCTTCGCCATGTGATGACGAATCTAGGTGAGAAACTGACTGATGAAGAGGTCGAGGAGA 602
|||| |||||||||||||| ||||| |||||||| || |||||||| || || || ||||
Sbjct 339 AGCTACGCCATGTGATGACCAATCTTGGTGAGAAGCTAACTGATGAGGAAGTTGAAGAGA 398
Query 603 TGATTCGTGAAGCAGATGTTGATGGAGATGGTCAGATAAACTACGAGGAGTTTGTCAAGA 662
|||| ||||| || ||||||||||||||||||||||| ||||| ||||||||||||||||
Sbjct 399 TGATCCGTGAGGCTGATGTTGATGGAGATGGTCAGATTAACTATGAGGAGTTTGTCAAGA 458
Query 663 TTATGATGGCTAAGTGATGATGATAA 688
|||||||||||||||||| ||||||
Sbjct 459 TTATGATGGCTAAGTGATT-TGATAA 483
Score = 119 bits (64), Expect = 4e-26
Identities = 78/85 (92%), Gaps = 0/85 (0%)
Strand=Plus/Plus
Query 191 GAAGAAGCTATGGCGGATCAGCTAACTGATGAACAGATCTCTGAGTTTAAGGAAGCTTTT 250
|||||||||||||||||||| || ||||| ||||||||||| || || ||||||||||||
Sbjct 17 GAAGAAGCTATGGCGGATCAACTCACTGACGAACAGATCTCCGAATTCAAGGAAGCTTTT 76
Query 251 AGCCTCTTCGACAAAGATGGAGATG 275
|||||||||||||||||||| ||||
Sbjct 77 AGCCTCTTCGACAAAGATGGCGATG 101
> Ahg489030 jgi|Araly1|489030|fgenesh2_kg.6__2164__AT5G21274.1
Length=634
Score = 363 bits (196), Expect = 2e-99
Identities = 324/385 (84%), Gaps = 11/385 (3%)
Strand=Plus/Plus
Query 301 GTG-TGGTTGCATCACCACAAAGGAGCTGGGAACAGTGATGCGGTCACTAGGGCAGAACC 359
||| ||||||||||||||| |||||||| ||||| |||||| |||| | ||||| ||||
Sbjct 88 GTGATGGTTGCATCACCACCAAGGAGCTCGGAACCGTGATGAGGTCTTTGGGGCAAAACC 147
Query 360 CAACAGAAGCTGAGCTACAAGACATGATCAACGAGGTTGACGCTGATGGAAACGGAACCA 419
||||||||||||||||||||||||||||||| || ||||| || ||||| ||||| ||||
Sbjct 148 CAACAGAAGCTGAGCTACAAGACATGATCAATGAAGTTGATGCAGATGGGAACGGGACCA 207
Query 420 TTGACTTCCCTGAGTTCTTAAACCTAATGGCTAA--AAAGATGAAGGACACAGACTCGGA 477
| || ||||| |||||| | ||||| ||||| || ||| ||||||||||| || || ||
Sbjct 208 TAGATTTCCCGGAGTTCCTGAACCTGATGGC-AAGGAAA-ATGAAGGACACTGATTCAGA 265
Query 478 GGAAGAGCTGAAAGAAGC-TTTCAGGGTGTTCGACAAAGACCAGAACGGTTTCATATCCG 536
|||||||||||| ||||| ||| ||||| || || ||||||||||| ||||||||||| |
Sbjct 266 GGAAGAGCTGAAGGAAGCCTTT-AGGGTTTTTGATAAAGACCAGAATGGTTTCATATCTG 324
Query 537 CTGCAGAGCTTCGCCATGTGATGACGAATCTAGGTGAGAAACTGA-CTGATGAAGAGGTC 595
| ||||||||||| ||||||||||| ||||| || ||||| || | | ||||| || ||
Sbjct 325 CAGCAGAGCTTCGACATGTGATGACAAATCTCGGGGAGAAGCT-ATCCGATGAGGAAGTT 383
Query 596 GAGGAGATGATTCGTGAAGCAGATGTTGATGGAGATGGTCAGATAAACTACGAGGAGTTT 655
|| |||||||| || ||||| ||||||||||| ||||||||||| ||||| || || ||
Sbjct 384 GATGAGATGATCCGAGAAGCGGATGTTGATGGCGATGGTCAGATCAACTATGAAGAATTC 443
Query 656 GTCAAGATT-ATGATGGCTAAGTGA 679
||||| | | |||||||||||||||
Sbjct 444 GTCAA-AGTCATGATGGCTAAGTGA 467
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 31451406525
Database: halleri_transcript_ahg.fa
Posted date: Sep 25, 2014 2:02 AM
Number of letters in database: 38,939,717
Number of sequences in database: 32,672
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5