BLASTN 2.2.26+ Reference: Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000), "A greedy algorithm for aligning DNA sequences", J Comput Biol 2000; 7(1-2):203-14. Database: halleri_transcript_ahg.fa 32,672 sequences; 38,939,717 total letters Query= AT4G23170.1 Length=1065 Score E Sequences producing significant alignments: (Bits) Value Ahg492547 jgi|Araly1|492547|fgenesh2_kg.7__1980__AT4G23150.1 904 0.0 Ahg889850 jgi|Araly1|889850|Al_scaffold_0007_1836 569 1e-161 Ahg889852 jgi|Araly1|889852|Al_scaffold_0007_1838 556 9e-158 Ahg329316 jgi|Araly1|329316|fgenesh1_pm.C_scaffold_7001543 505 3e-142 Ahg492546 jgi|Araly1|492546|fgenesh2_kg.7__1979__AT4G23180.1 243 3e-63 > Ahg492547 jgi|Araly1|492547|fgenesh2_kg.7__1980__AT4G23150.1 Length=1656 Score = 904 bits (489), Expect = 0.0 Identities = 675/765 (88%), Gaps = 12/765 (2%) Strand=Plus/Plus Query 113 ATGTCTTCTCTGATCTCTTTTATCTTCCTTTTCCTTTTCTCATTCCTCACTAGC-TTCAA 171 ||||||||||||| | |||| ||||||||||||||||||||||||||||||| | |||| Sbjct 1 ATGTCTTCTCTGACCACTTTCATCTTCCTTTTCCTTTTCTCATTCCTCACTA-CTTTCAC 59 Query 172 AGCTTCCGCTCAAGATCCTTTTTACTTAAATC-ATTATTGTCCAAATACGACAACTTACT 230 ||||| |||||||| ||| | || | | | ||| |||||||||| ||||||||||| Sbjct 60 TGCTTCTGCTCAAGAACCTAATGACCT-AGCCTATTGGTGTCCAAATAGGACAACTTACT 118 Query 231 CAAGAT-TCAGCACTTACTCCACCAATCTCAGAACCCTTTTGTCCTCT-TTTGCTTCCCG 288 |||| | ||||||||||| ||||||||| | ||||||||| |||||| | | |||||| Sbjct 119 CAAG-TAACAGCACTTACTTCACCAATCTTAAAACCCTTTTTTCCTCTCTCT-CTTCCCC 176 Query 289 CAACGCCTCTTACTCCACCGGATTCCAGAACGTCACGGTGGGACAAACCCCCGACCTGGT 348 |||||||||||||||||||||||| || |||| ||||||||||||| |||||||| ||| Sbjct 177 CAACGCCTCTTACTCCACCGGATTTCAAAACGCCACGGTGGGACAAGCCCCCGACAGGGT 236 Query 349 CACCGGACTTTTCCTCTGCCGCGGAGACCTCTCGCCGGAAGTTTGCAGTAACTGCGTTGC 408 ||| ||||||||||| |||||||||||| ||||||||||||||||| |||||||||| || Sbjct 237 CACTGGACTTTTCCTTTGCCGCGGAGACTTCTCGCCGGAAGTTTGCCGTAACTGCGTCGC 296 Query 409 CTTCTCCGTTGACGAAGCGTTAACTAGGTGTCCAAGTCAGAGAGAAGCTGTGTTCTATTA 468 ||||||||| ||||| |||||| | ||||||||| ||||||||||| |||||||||||| Sbjct 297 TTTCTCCGTTAACGAATCGTTAATTCGGTGTCCAAATCAGAGAGAAGTTGTGTTCTATTA 356 Query 469 CGAGGAGTGTATACTCAGATACTCTGACAAGAATATTCTATCGACGGCCATTACAAACGA 528 ||| || || || |||||||||||| |||||||||||||||||||||||| |||||||| Sbjct 357 CGATGAATGCATGCTCAGATACTCTCACAAGAATATTCTATCGACGGCCAACACAAACGA 416 Query 529 AGGAGAATTTATCTTGA-GTAACACCAACACTATTTCTCCTAATCAAAGTCAAATAAACC 587 |||||||| |||| ||| | ||| | || | ||||||||||| ||||| ||||| ||||| Sbjct 417 AGGAGAATATATCATGACG-AACGCTAATAATATTTCTCCTATTCAAAATCAAACAAACC 475 Query 588 AGTTTATAGTTTTGGTGCAATCTAATATGAACCAAGCTGCCATGGAAGCAGCCAACAGTT 647 |||||||| |||||||| ||||||||||||||||||||||| ||||||||||||||||| Sbjct 476 AGTTTATAATTTTGGTGTTATCTAATATGAACCAAGCTGCCACGGAAGCAGCCAACAGTT 535 Query 648 CTAGAAAATTCTCTACGATAAAGACCGAATTGACCGAACTCCAGACTTTGTACGGGCTTG 707 |||||||||||||||||||||||||||||||||||| ||||||||||||||||||||| | Sbjct 536 CTAGAAAATTCTCTACGATAAAGACCGAATTGACCGCACTCCAGACTTTGTACGGGCTCG 595 Query 708 TTCAATGCACTCCTGATCTTACAAGTCAAGACTGTTTGAGATGTCTGACAAGATCCATCA 767 |||||||||||||||||||| |||| || |||| |||| ||||||||||| ||||||| Sbjct 596 TTCAATGCACTCCTGATCTTTCAAGAGAAAACTGCATGAGTTGTCTGACAAGTTCCATCA 655 Query 768 ATAGAATGCCACTTTCTAGAATTGGAGCAAGACAGT-TTTGGCCAAGTTGTAATTCAAGG 826 ||||| ||||| |||||||||||||||||||||| | ||||||||||||||||||||||| Sbjct 656 ATAGACTGCCATTTTCTAGAATTGGAGCAAGACA-TCTTTGGCCAAGTTGTAATTCAAGG 714 Query 827 TACGAGCTTTACTCGTTCTACAACGAAACTACCATTGGAACACCA 871 |||||||||||| |||||||| ||||||| ||||||| |||||| Sbjct 715 TACGAGCTTTACGCGTTCTACGACGAAACCGCCATTGGTACACCA 759 > Ahg889850 jgi|Araly1|889850|Al_scaffold_0007_1836 Length=1821 Score = 569 bits (308), Expect = 1e-161 Identities = 425/481 (88%), Gaps = 9/481 (2%) Strand=Plus/Plus Query 113 ATGTCTTCTCTGATCTCTTTTATCTTCCTTTTCCTTTTCTCATTCCTCACTAGCTTCAAA 172 ||||||||| | |||| || | |||||||||||||||||| ||||||| |||||||| | Sbjct 1 ATGTCTTCTTTCTTCTCCTTCACCTTCCTTTTCCTTTTCTCCTTCCTCATTAGCTTCAGA 60 Query 173 GCTTCCGCTCAAGATCCTTTTTA-CTTAAATCATTATTGTCCAAATACGACAACTTACTC 231 ||||| |||||| | |||| ||| ||| |||||| ||||||||||||||||||||||||| Sbjct 61 GCTTCTGCTCAAAAACCTTATTACCTT-AATCATAATTGTCCAAATACGACAACTTACTC 119 Query 232 AAG-ATTCAGCACTTACTCCACCAATCTCAGAACCCTTTTGTCCTCTTT-TGCTTCCCGC 289 ||| | ||||||||||||||||||||||| |||||||||||||||| | | |||||||| Sbjct 120 AAGTAA-CAGCACTTACTCCACCAATCTCAAAACCCTTTTGTCCTCTCTAT-CTTCCCGC 177 Query 290 AACGCCTCTTACTCCACCGGATTCCAGAACGTCACGGTGGGACAAACCCCCGACCTGGTC 349 ||||||||||| |||||||||||||| |||| |||||||||||||||||||||| |||| Sbjct 178 AACGCCTCTTATTCCACCGGATTCCAAAACGCCACGGTGGGACAAACCCCCGACAGGGTC 237 Query 350 ACCGGACTTTTCCTCTGCCGCGGAGACCTCTCGCCGGAAGTTTGCAGTAACTGCGTTGCC 409 |||||||||||||| |||||||||||||||||||| ||||||||| |||||||||||||| Sbjct 238 ACCGGACTTTTCCTTTGCCGCGGAGACCTCTCGCCCGAAGTTTGCCGTAACTGCGTTGCC 297 Query 410 TTCTCCGTTGACGAAGCGTTAACTAGGTGTCCAAGTCAGAGAGAAGCTGTGTTCTATTAC 469 ||||||||| ||||| |||||||| ||||||||| |||||||||||||||||||||||| Sbjct 298 TTCTCCGTTAACGAATCGTTAACTCGGTGTCCAAATCAGAGAGAAGCTGTGTTCTATTAT 357 Query 470 GAGGAGTGTATACTCAGATACTCTGACAAGAATATTCTATCGACGGCCATTACAAACGAA 529 |||||||| ||||| ||||||||| ||||||||||||||||||| | ||||| ||||| Sbjct 358 GAGGAGTGCATACTTAGATACTCTCACAAGAATATTCTATCGACAG---TTACATACGAA 414 Query 530 GGAGAATTTATCTTGAGTAACACCAACACTATTTCTCCTAATCAAAGTCAAATAAACCAG 589 |||||| |||| |||| ||| |||| | ||||||| ||| ||||| ||||| | ||||| Sbjct 415 GGAGAACATATCATGAGGAACCCCAATAATATTTCTTCTATTCAAAATCAAAGAGACCAG 474 Query 590 T 590 | Sbjct 475 T 475 > Ahg889852 jgi|Araly1|889852|Al_scaffold_0007_1838 Length=2088 Score = 556 bits (301), Expect = 9e-158 Identities = 599/736 (81%), Gaps = 47/736 (6%) Strand=Plus/Plus Query 138 TCCTTTTCCTTTTCTCATTCCTCACTAGCTTCAAAGCTTCCGCTCAAG-ATCCT--TTTT 194 |||||||||||||||| |||||||||||||||||||||| |||| || | ||| ||| Sbjct 26 TCCTTTTCCTTTTCTCCCTCCTCACTAGCTTCAAAGCTTCTGCTC-AGAAGCCTACTTTC 84 Query 195 ACTTAAATC-ATTATTGTCCAAATACGACAACTTACTCAAG-ATTCAGCACTTACTCCAC 252 || | | | || |||||||||||||||||||||||||||| | ||||||||||| ||| Sbjct 85 AC--ATA-CGATCATTGTCCAAATACGACAACTTACTCAAGGAA-CAGCACTTACTTCAC 140 Query 253 CAATCTCAGAACCCTTTTGTCCTCTTT-TGCTTCCCGCAACGCCTCTTACTCCACCGGAT 311 |||||||| ||||||||| || ||| | | || ||| ||| ||||||||||||||||||| Sbjct 141 CAATCTCAAAACCCTTTTATCGTCTCTCTCCTCCCC-CAATGCCTCTTACTCCACCGGAT 199 Query 312 TCCAGAACGTCACGGTGGGACAAACCCCCGACCTGGTCACCGGACTTTTCCTCTGCCGCG 371 |||| |||| || |||||||||| |||||||| |||||| ||||||||||| ||||||| Sbjct 200 TCCAAAACGCCAAGGTGGGACAAGCCCCCGACAGGGTCACTGGACTTTTCCTTTGCCGCG 259 Query 372 GAGACCTCTCGCCGGAAGTTTGCAGTAACTGCGTTGCCTTCTCCGTTGACGAAGCGTTAA 431 ||||||||||||| ||||||| | |||||||| | |||||||||||| ||||| |||||| Sbjct 260 GAGACCTCTCGCCCGAAGTTTTCCGTAACTGCATCGCCTTCTCCGTTAACGAAACGTTAA 319 Query 432 CTAGGTGTCC-AAGTCAGAGAGAAGCTGTGTTCTATTACGAGGAGTGTATACTCAGATAC 490 || ||||||| || |||||||||||| ||| |||||||||||||||| || ||||||||| Sbjct 320 CTCGGTGTCCGAA-TCAGAGAGAAGCCGTGCTCTATTACGAGGAGTGCATGCTCAGATAC 378 Query 491 TCTGACAAGAATATTCTATCGACGGCCATTACAAACGAAGGAGAATTTATCTTGA-GTAA 549 ||| ||||||||||||||||||| || ||| || | |||| ||| ||| ||| | || Sbjct 379 TCTCACAAGAATATTCTATCGAC--CC-TTATTTACCATGGAGGATTCATCATGACG-AA 434 Query 550 CACCAACACTATTTC-TCCTAATCAAAGTCAAATAAACCAGTTTATA-G-TTTTGG-TGC 605 | |||| | |||||| || | ||| | | | | ||| | || | | ||||| | | Sbjct 435 CCCCAATAATATTTCATC-T---CAAC-T-AGA-AGACC-GATTCGAAGATTTTGTAT-C 485 Query 606 AATCTAATATGAACCAAGCTGCC-ATGGAAGCAGCCAACAGTTCTAGAAAATTCTCTACG 664 || | ||||||||||||||| | ||| |||| || | |||||| || |||| ||| Sbjct 486 G-TCCACGATGAACCAAGCTGCCGA-GGAGGCAGTGAATAATTCTAGGAAGTTCTATACA 543 Query 665 ATAAAGACCGAATTG-ACCGAACTCCAGACTTTGTACGGGCTTGTTCAATGCACTCCTGA 723 |||| | || || || |||| ||||| || |||| ||| || ||||||||||||||||| Sbjct 544 ATAAGGGCC-AACTGGACCGCACTCCGGAGTTTGCACGTACTGGTTCAATGCACTCCTGA 602 Query 724 TCTTACAAGTCAAGACTGTTTGAGA-TGTCTGACAAGA-TCCATCAATAGAATGCCACTT 781 ||||| ||| |||||||| ||| | |||||| ||| | ||||||||| ||||||| ||| Sbjct 603 TCTTAGAAGACAAGACTGCTTG-GCCTGTCTG-CAATACTCCATCAATGGAATGCCTCTT 660 Query 782 T-CTAGAATTGGAGCAAGACAGTT-T-TGGCCAAGTTGTAATTCAAGGTACGAGCTTTAC 838 | | ||||| |||| ||||| || | |||||||| ||||| |||||||||||||||||| Sbjct 661 TAC-AGAATCGGAGGAAGAC--TTCTCTGGCCAAGCTGTAACTCAAGGTACGAGCTTTAC 717 Query 839 TCGTTCTACAACGAAA 854 ||||||||||||||| Sbjct 718 CCGTTCTACAACGAAA 733 > Ahg329316 jgi|Araly1|329316|fgenesh1_pm.C_scaffold_7001543 Length=666 Score = 505 bits (273), Expect = 3e-142 Identities = 316/337 (94%), Gaps = 2/337 (1%) Strand=Plus/Plus Query 547 TAACACCAACACTATTTCTCCTAATCAAAGTCAAATAAACCAGTTTATAGTTTTGGTGCA 606 ||||||||| | ||||||||||| ||||| |||||||||||||||||| |||||||| Sbjct 303 TAACACCAATAATATTTCTCCTATTCAAAAACAAATAAACCAGTTTATAATTTTGGTGTT 362 Query 607 ATCTAATATGAACCAAGCTGCCATGGAAGCAGCCAACAGTTCTAGAAAATTCTCTACGAT 666 |||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||| Sbjct 363 ATCTAATATGAACCAAGCTGCCATGGAAGCAGCCAAAAGTTCTAGAAAATTCTCTACGAT 422 Query 667 AAAGACCGAATTGACCGAACTCCAGACTTTGTACGGGCTTGTTCAATGCACTCCTGATCT 726 || |||||||||||||| |||||||||||||||||||||||||||||||||||||||||| Sbjct 423 AACGACCGAATTGACCGCACTCCAGACTTTGTACGGGCTTGTTCAATGCACTCCTGATCT 482 Query 727 TACAAGTCAAGACTGTTTGAGATGTCTGACAAGATCCATCAATAGAATGCCACTTTCTAG 786 |||||| |||||||| |||||||||||||||| |||||||||||||||||||||||||| Sbjct 483 TACAAGACAAGACTGCATGAGATGTCTGACAAGCTCCATCAATAGAATGCCACTTTCTAG 542 Query 787 AATTGGAGCAAGACAGTTTTGGCCAAGTTGTAATTCAAGGTACGAGCTTTACTCGTTCTA 846 |||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||| Sbjct 543 AATTGGAGCAAGACAGTTTTGGCCAAGTTGTAATTCAAGGTACGAGCTTTACGCGTTCTA 602 Query 847 CAACGAAACTACCATTG-GAACACCATCTCCACCACC 882 ||||||||| |||||| || ||||||| |||||||| Sbjct 603 CAACGAAACCGCCATTGAGA-CACCATCACCACCACC 638 Score = 364 bits (197), Expect = 6e-100 Identities = 273/308 (89%), Gaps = 11/308 (4%) Strand=Plus/Plus Query 113 ATGTCTTCTCTGATCTCTTTTATCTTCCTTTTCCTTTTCTCATTCCTCACTAGCTTCAAA 172 ||||||||||| || ||||| |||||||| ||||||||||| |||||||||||||||||| Sbjct 1 ATGTCTTCTCTTATATCTTTCATCTTCCTCTTCCTTTTCTCCTTCCTCACTAGCTTCAAA 60 Query 173 GCTTCCGCTCAAGATCCTT-TTTACTTAAATCATTATTGTCCAAATACGACAACTTACTC 231 ||||| |||||||||| || ||| | || ||||| ||||||||||||||||||||||||| Sbjct 61 GCTTCTGCTCAAGATCATTATTT-CCTATATCATAATTGTCCAAATACGACAACTTACTC 119 Query 232 AAG-ATTCAGCACTTACTCCACCAATCTCAGAACCCTTTTGTCCTCTTT-TGCTT-CCCG 288 ||| | ||||||||||| ||||||||||| ||| |||||||||||| | | ||| ||| Sbjct 120 AAGTAA-CAGCACTTACTTCACCAATCTCAAAACTCTTTTGTCCTCTCTCT-CTTTCCC- 176 Query 289 CAACGCCTCTTACTCC-ACCGGATTCCAGAACGTCACGGTGGGACAAACCCCCGACCTGG 347 |||||||||||| ||| ||||||||||| |||| |||||||||||||||||| ||| || Sbjct 177 CAACGCCTCTTA-TCCCACCGGATTCCAAAACGCCACGGTGGGACAAACCCCTGACAGGG 235 Query 348 TCACCGGACTTTTCCTCTGCCGCGGAGACCTCTCGCCGGAAGTTTGCAGTAACTGCGTTG 407 |||||||||||||||| |||||||||||||||| ||| ||||||||| |||||||||| | Sbjct 236 TCACCGGACTTTTCCTTTGCCGCGGAGACCTCT-GCCCGAAGTTTGCCGTAACTGCGTCG 294 Query 408 CCTTCTCC 415 |||||||| Sbjct 295 CCTTCTCC 302 > Ahg492546 jgi|Araly1|492546|fgenesh2_kg.7__1979__AT4G23180.1 Length=2181 Score = 243 bits (131), Expect = 3e-63 Identities = 588/798 (74%), Gaps = 73/798 (9%) Strand=Plus/Plus Query 127 CTCTTT-TATCTTCCTTTTCCTTTTCTCATTCCTCACTAGCTTCAAAGCTTCCGCTCAAG 185 |||||| | ||||||||||||||||||| || |||||||| |||| || ||| ||||||| Sbjct 92 CTCTTTCT-TCTTCCTTTTCCTTTTCTCCTTTCTCACTAGTTTCAGAGTTTCTGCTCAAG 150 Query 186 ATCCTTTTTACTTAAATCATTAT-TGTCCAAATACGACAACTTACTCAAGAT-TCAGCAC 243 |||| |||| || |||| || ||| |||||||| ||| |||||| || | |||| | Sbjct 151 ATCCCACTTACCTATATCA-CATCTGTTCAAATACGGCAAATTACTCGAG-TAACAGC-C 207 Query 244 -TTACTCCACCAATCTCAGAACCCTTTTGTCCTCT-TTTGCTTCCCGCAACGCCTCTTAC 301 |||| |||||||||||||||||||||| | ||| ||| |||||||||||||||||||| Sbjct 208 TTTACAACACCAATCTCAGAACCCTTTTGGCTTCTCTTT-CTTCCCGCAACGCCTCTTAC 266 Query 302 TCCACCGGATTCCAGAACGTCACGGT-GGGACAAACCCCCGACCTG-GTCACCGGACTTT 359 |||||||| ||||| |||| ||| || ||| ||| | || ||| | ||||||||||||| Sbjct 267 TCCACCGGCTTCCAAAACGCCACCGTCGGG-CAAGCTCCAGACA-GAGTCACCGGACTTT 324 Query 360 TCC-TCTGCCGCGGAGACCTCTCGCCGGAAGTTTGCA---GTAA-C--TGCGTTGCCTTC 412 | | ||||| ||||| ||||| | ||||||||| || | ||||| |||| Sbjct 325 TTAAT-TGCCGTGGAGATTTCTCGACAGAAGTTTGCCTTTGTCGTCGCTGCGTCACCTT- 382 Query 413 TCC-GTTGACGA-AGCGTTAACTAGGTGTCC-AAGTCAGAGAGAAG-CTGTGTTCTATTA 468 | | || |||| | | |||| | ||||| | || | ||| ||| | | | ||| || Sbjct 383 TGCTGTCAACGACACC-TTAAGTCGGTGTTCGAA-TGAGAAAGAGGTCACAAT-CTACTA 439 Query 469 CGAGGAGTGTATACTCAGATACTCTGACAAGAATATTCTATCGACGGCCATTA-CAAACG 527 ||| ||||||| || ||||||||| || ||| || || || || || | | || || Sbjct 440 CGATCAGTGTATGCTTAGATACTCTAACGGGAACATACTCTCAAC--CC-TAAGCAC-CG 495 Query 528 AAGGAGAA-T-TT-ATCTTGAGTAACACCAACACTATTTCTCCTAATCAAAGTCAAATAA 584 |||| | | || || | | | ||| || || || | | | | || || ||| || Sbjct 496 GTGGAGGAGTGTTTATGT-G-G-AAC-CCT-CAGAATGT-TAC-A-TCTAAC-CAACTAG 546 Query 585 ACCAGTTTATAGTTTTGGT-GCAATCTAATATGAACCAAGCTGCCA-TGGAAGCAGCCAA 642 |||| || | || |||||| || | | | ||||||||||| ||| || |||| |||| Sbjct 547 ACCAATTCAGAGATTTGGTCGCGACC-ACGATGAACCAAGCCGCCGCTG-AAGCTGCCAG 604 Query 643 CAGTTCTAGAAAATTCTC-TACGATAAA-GACCGAA-TTGACCGAACTCCAGACTTTGTA 699 || ||| |||||||| | | | | ||| | || || || || | | |||| ||||| Sbjct 605 CAATTCCAGAAAATT-TGATGCCAGAAAAG-CC-AACTTCACTGCGTTGCAGAAGTTGTA 661 Query 700 CGGGCTTGTTCAATGCACTCCTGATCTTACAAGTCAAGACTGTTTGAGATGTCTGACAA- 758 ||| || ||||| |||||||||||||| || || |||||||||||||| ||||| |||| Sbjct 662 CGGACTGGTTCAGTGCACTCCTGATCTGACGAGACAAGACTGTTTGAGTTGTCT-ACAAC 720 Query 759 -GATCCATCAATAGAAT-GCC-ACTTTCTAGAATTGGAGCAAGACAGTTT-TG--GCCA- 811 || | |||||| ||| ||| ||| || |||||| | |||| ||| || || | Sbjct 721 AGAGC-ATCAATC-AATTGCCCACTGA-TAAAATTGGGGGAAGA---TTTATGATGC-AG 773 Query 812 AGTTGTAATTCAAGGTACGAGCTTTACTCGTTCTACAACGAAACTACCATTGGAACACCA 871 || |||| |||||| | |||||||||| |||| ||||||||| | ||||| ||||| Sbjct 774 AGCTGTAGTTCAAGATTCGAGCTTTACGCGTTTTACAACGAATCCGCCATTACTACACCT 833 Query 872 TCTCCACCACCACCACCG 889 || |||||||| |||||| Sbjct 834 TCACCACCACCTCCACCG 851 Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 39647833680 Database: halleri_transcript_ahg.fa Posted date: Sep 25, 2014 2:02 AM Number of letters in database: 38,939,717 Number of sequences in database: 32,672 Matrix: blastn matrix 1 -2 Gap Penalties: Existence: 0, Extension: 2.5