BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: halleri_transcript_ahg.fa
32,672 sequences; 38,939,717 total letters
Query= AT4G35360.1
Length=1380
Score E
Sequences producing significant alignments: (Bits) Value
Ahg491111 jgi|Araly1|491111|fgenesh2_kg.7__544__AT4G35360.1 2180 0.0
Ahg480618 jgi|Araly1|480618|fgenesh2_kg.3__3319__AT2G17340.1 1251 0.0
Ahg931587 jgi|Araly1|931587|scaffold_304044.1 1164 0.0
> Ahg491111 jgi|Araly1|491111|fgenesh2_kg.7__544__AT4G35360.1
Length=1309
Score = 2180 bits (1180), Expect = 0.0
Identities = 1268/1311 (97%), Gaps = 4/1311 (0%)
Strand=Plus/Plus
Query 71 TCACACGTACGAACGAAGAATGGAGAGCGACTCCGAGATGGTAGCGTTGCCTCTGTTACC 130
|||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
Sbjct 1 TCACACGTACGAACGAAGAATGGAGAGCGACTCCGAGATGGTTGCGTTGCCTCTGTTACC 60
Query 131 GACTCCGATAGAATCGAACTACAGGGCTTGCACCATTCCTTATCGATTCCCTTCTGATAA 190
||| ||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
Sbjct 61 GACGCCGATAGAATCAAACTACAGGGCTTGCACCATTCCTTATCGATTCCCTTCTGATAA 120
Query 191 TCCTAGAAAGGCTACTCCCACTGAGATCTCCTGGATCGATCTCTTCTCCAATTCCATCCC 250
|||||||||||||||||| ||||||||||| |||||||||||||||||||||||||||||
Sbjct 121 TCCTAGAAAGGCTACTCCTACTGAGATCTCTTGGATCGATCTCTTCTCCAATTCCATCCC 180
Query 251 TTCTTTCAAGGAACGTGCAGAGAGCGATACAACCGTTCCAGATGCTCCTGTTCGAGCTGA 310
|||||||||||||||||||||||| || ||||||||||||||||||||||||||||||||
Sbjct 181 TTCTTTCAAGGAACGTGCAGAGAGTGACACAACCGTTCCAGATGCTCCTGTTCGAGCTGA 240
Query 311 AAAGTTTGCCAAAAGGTATGCGGAGATTCTTGAGGACCTGAAGAAAGATCCGGAGAGCCA 370
||| ||||||||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 241 AAAATTTGCCAAAAGGTATGCGGAGATTCTTGAGGACTTGAAGAAAGATCCGGAGAGCCA 300
Query 371 TGGTGGTCCACCGGATTGCATTCTTCTCTGCAGAATTCGTGAGCTGATACTAAGAGAATT 430
|||||||||||| ||||||||||||||||||||||| |||||||| ||||||||||||||
Sbjct 301 TGGTGGTCCACCAGATTGCATTCTTCTCTGCAGAATCCGTGAGCTAATACTAAGAGAATT 360
Query 431 GGGATTTAGGGACATATTTAAGAAAGTTAAGGATGAGGAGAATGCAAAGGCAATATCACT 490
|||||||||||||||||| |||||||||||||| || |||||||||||||| ||||||||
Sbjct 361 GGGATTTAGGGACATATTCAAGAAAGTTAAGGACGACGAGAATGCAAAGGCTATATCACT 420
Query 491 ATTTCCTGAAGTTGTCCGTTTGAGTGATGCTATTAACGATGAAGGCAAGCGCATAGAGAA 550
|||||| ||||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 421 ATTTCCAGAAGTTGTCCGTTTGAGTGATGCTATTGACGATGAAGGCAAGCGCATAGAGAA 480
Query 551 TCTGGTCAGAGGGATATTTGCTGGAAACATCTTTGATCTGGGTTCTGCACAGCTTGCTGA 610
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 481 TCTGGTCAGAGGGATATTTGCTGGAAACATCTTTGATCTGGGTTCTGCACAGCTTGCTGA 540
Query 611 GGTTTTCTCAAAAGACGGCATGTCCTTTTTGGCTAGCTGTCAGAACTTGGTTTCACGACC 670
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 541 GGTTTTCTCAAAAGACGGCATGTCCTTTTTGGCTAGCTGTCAGAACTTGGTTTCACGACC 600
Query 671 ATGGGTCATTGATGACTTGGATAACTTTCAAGCTAGATGGCTCAAGAAGCCTTGGAAGAA 730
|||||||||||||||||||||||||||||||||||||||| |||| ||||||||||||||
Sbjct 601 ATGGGTCATTGATGACTTGGATAACTTTCAAGCTAGATGGGTCAAAAAGCCTTGGAAGAA 660
Query 731 GGCTGTGATATTTGTCGATAATTCTGGTGCAGACATAATTTTGGGTATCTTGCCCTTTGC 790
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 661 GGCTGTGATATTTGTCGATAATTCTGGTGCAGACATAATTTTGGGTATCTTGCCCTTTGT 720
Query 791 TAGAGAAATGCTACGTCTAGGAATGCAGGTTGTACTGGCTGCTAATGAGCTTCCGTCTAT 850
||||||||||||||||| ||||||||||||||| |||||||||||||||||||| |||||
Sbjct 721 TAGAGAAATGCTACGTCGAGGAATGCAGGTTGTGCTGGCTGCTAATGAGCTTCCATCTAT 780
Query 851 CAACGATGTAACTTATATCGAGCTTGCAGAGATCTTGTCTAAGCTGAATGATGAAAATGG 910
|||||||||||||||||||||||||||||||||||||||||||||||| |||||||| ||
Sbjct 781 CAACGATGTAACTTATATCGAGCTTGCAGAGATCTTGTCTAAGCTGAAGGATGAAAAAGG 840
Query 911 ACAATTGATGGGTGTTGATACCTCAAATCTTCTTATTGCAAATTCAGGGAATGACTTACC 970
|||||||||||||||||||||||| |||||||||||||||||||||||||| ||||||||
Sbjct 841 ACAATTGATGGGTGTTGATACCTCGAATCTTCTTATTGCAAATTCAGGGAACGACTTACC 900
Query 971 GGTTATTGATCTTGCAAGAGTATCACAGGAGGTTGCGTACCTCTCAAGCGATGCAGACTT 1030
||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
Sbjct 901 GGTTATTGATCTTGCAAGAGTATCACAGGAGGTTGCGTACCTCTCAACCGATGCAGACTT 960
Query 1031 GGTCATCTTAGAAGGCATGGGCCGTGGAATAGAGACAAACCTTTATGCTCAGTTCAAGTG 1090
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 961 GGTCATCTTAGAAGGCATGGGCCGTGGAATAGAGACAAACCTTTATGCTCAGTTCAAGTG 1020
Query 1091 TGATTCCCTCAAGATCGGAATGGTGAAGCATCCAGAAGTAGCACAGTTTCTCGGAGGACG 1150
||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1021 TGATTCCCTCAAGATTGGAATGGTGAAGCATCCAGAAGTAGCACAGTTTCTCGGAGGACG 1080
Query 1151 GCTTTATGACTGTGTCATCAAATACAATGAAGTTCAGATTTAATTATATTTCACA--CAC 1208
|||||||||||||||||||||||||||||||||||||| |||||||||||||||| |||
Sbjct 1081 GCTTTATGACTGTGTCATCAAATACAATGAAGTTCAGAGTTAATTATATTTCACAAACAC 1140
Query 1209 ATTTTTACTAAACCAGCCATTCAGTTTATTATATTAAAAACTGTTAGGCTTTGTAAGTTA 1268
||||||||| |||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 1141 ATTTTTACTGAACCAGCCATTCAGTTTATTATATGAAAAACTGTTAGGCTTTGTAAGTTA 1200
Query 1269 GAGATCCAATCTCTATCTCTGCATTCAGATGTAAGAATTGAAAACTCTTTCGACATTCTT 1328
|||||||| ||||||||||| ||||||||||||||||||||||| |||||||||||||||
Sbjct 1201 GAGATCCACTCTCTATCTCTACATTCAGATGTAAGAATTGAAAAATCTTTCGACATTCTT 1260
Query 1329 TATCAAACACACACAAAAAGCACCAATCTTTCTGATTGGACCTTATCTGGT 1379
||| ||||||||| |||| |||||||||||||||||||| |||||| ||||
Sbjct 1261 TATTAAACACACA-AAAA-GCACCAATCTTTCTGATTGGTCCTTATATGGT 1309
> Ahg480618 jgi|Araly1|480618|fgenesh2_kg.3__3319__AT2G17340.1
Length=1268
Score = 1251 bits (677), Expect = 0.0
Identities = 984/1131 (87%), Gaps = 25/1131 (2%)
Strand=Plus/Plus
Query 85 GAAGAATGGAGAGCGACTCCGAGATGGTAGCGTTGCCTCTGTTACCGACTCCGATAGAAT 144
||| |||||||||||| || |||||||| |||| |||| |||||||| |||||||||
Sbjct 28 GAA-AATGGAGAGCGATTCAGAGATGGTTCCGTTTCCTCAATTACCGACGCCGATAGAAA 86
Query 145 CGAACTACAGGGCTTGCACCATTCCTTATC-GATTCCCTTCTGATAAT-CCTAGAAAGGC 202
| |||||||| ||||||||||||||||| | |||||||||||||| || || ||||| ||
Sbjct 87 CCAACTACAGAGCTTGCACCATTCCTTA-CAGATTCCCTTCTGATGATCCCAAGAAA-GC 144
Query 203 TACTCCCACTGAGATCTCCTGGATCGATC-TCTTCTCCAATTCCATCCCTTCTTTCAAGG 261
|||||| ||||| ||||| |||||| | | ||||| |||||||||| ||||||||||||
Sbjct 145 TACTCCTACTGAAATCTCGTGGATC-AACGTCTTCGCCAATTCCATTCCTTCTTTCAAGA 203
Query 262 AACGTGCAGAGAGCGATACAACCGTTCCAGATGCTCCTGTTCGAGCTGAAAAGTTTGCCA 321
|||||||||||||||||||||| |||||||||||||||| | |||||||||| |||| ||
Sbjct 204 AACGTGCAGAGAGCGATACAACGGTTCCAGATGCTCCTGCTAGAGCTGAAAATTTTG-CA 262
Query 322 -AAAGGTATGC-GGAGATTCTTGAGGACCTGAAGAAAGATCCGGAGAGCCATGGTGGTCC 379
|||| ||||| ||| |||||||| ||| ||||||||||||| ||||| ||||| || ||
Sbjct 263 GAAAGATATGCGGGA-ATTCTTGAAGACTTGAAGAAAGATCCAGAGAGTCATGGCGGGCC 321
Query 380 ACCGGATTGCATTCTTCTCTGCAGAATTCGTGAGCTGA-TACTAAGAGAATTGGGATTTA 438
||| ||| | |||||||| ||| || ||||||||| | |||| |||||||| |||||||
Sbjct 322 ACCAGATGGTATTCTTCTATGCCGACTTCGTGAGC-AAGTACTCAGAGAATTAGGATTTA 380
Query 439 GGGACATATTTAAGAAAGTTAAGGATGAGGAGAATGCAAAGGCAATATCACTATTTCCTG 498
|||||||||| |||||||| ||||||||||||||||||||||| ||||||||||||||||
Sbjct 381 GGGACATATTCAAGAAAGTGAAGGATGAGGAGAATGCAAAGGCTATATCACTATTTCCTG 440
Query 499 AAGTTGTCCGTTTGAGTGATGCTATT-AACGATGAAGGCAAGCGCATAGAGAATCTGGTC 557
|||||||| || |||||||||||||| || ||||| || || || |||||||| ||||
Sbjct 441 AAGTTGTCAGTCTGAGTGATGCTATTGAA-GATGACGGAAAACGGTTAGAGAATTTGGTG 499
Query 558 AGAGGGATATTTGCTGGAAACATCTTTGATCTGGGTTCTGCACAGCTTGCTGAGGTTTTC 617
||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||
Sbjct 500 AGAGGGATATTTGCTGGAAACATCTTTGATCTGGGTTCTGCACAGCTTGCTGAAGTTTTC 559
Query 618 TCAAAAGACGGCATGTCCTTT-TTGGCTAGCTGTCAGAACTTGGTTTCACGACCATGGGT 676
|||| || || ||||| ||| |||||||||||||| || |||||| |||||||||||||
Sbjct 560 TCAAGGGATGGTATGTC-TTTCTTGGCTAGCTGTCAAAATTTGGTTCCACGACCATGGGT 618
Query 677 CATTGATGACTTGGATAACTTTCAAGCTAGATGGCTCAAGAAGCCTTGGAAGAAGGCTGT 736
||||||||||||||| ||||| ||||| | |||| |||| ||| ||||||||||||| ||
Sbjct 619 CATTGATGACTTGGAAAACTTCCAAGCCAAATGGATCAACAAGTCTTGGAAGAAGGCAGT 678
Query 737 GATATTTGTCGATAATTCTGGTGCAGACATAATTTTGGGTATCTTGCCCTTTGCTAGAGA 796
||| ||||| |||||||||||||||||||||||||||||||| ||||| ||||| |||||
Sbjct 679 GATTTTTGTTGATAATTCTGGTGCAGACATAATTTTGGGTATTTTGCCGTTTGCAAGAGA 738
Query 797 AATGCTACGTCTAGGAA-TGCAGGTTGTACTGGCTGCTAATGAGCTTCCGTCTATCAACG 855
|||| |||| ||||| | |||||||| |||||||||||||| || || |||||||| |
Sbjct 739 GTTGCTCCGTCGAGGAACT-CAGGTTGTGCTGGCTGCTAATGAACTGCCATCTATCAATG 797
Query 856 ATGTAACTTATATCGAGCTTGCAGAGATCTTGTCTA-AGCTGAATGATGAAAATGGACAA 914
| |||| |||| ||||||| ||||||||||| | | || |||| ||||||||||| |||
Sbjct 798 ACATAACATATACCGAGCTTACAGAGATCTTGAC-ACAGTTGAAGGATGAAAATGGCCAA 856
Query 915 TTGATGGGTGTTGATACCTCAAATCTTCTTATTGCAAATTCAGGGAATGACTTACCGGTT 974
||| | || |||||||| || || ||||| ||||||||||| |||||||||||||| |||
Sbjct 857 TTGCTAGGCGTTGATACTTCGAAGCTTCTGATTGCAAATTCGGGGAATGACTTACCAGTT 916
Query 975 ATTGATCT-TGCAAGAGTATCACAGGAGGTTGCGTACCTCTCAAGC-GATGCAGACTTGG 1032
|| ||||| | ||||||||||||||||| |||| || || ||| | ||||| ||||| |
Sbjct 917 ATCGATCTCT-CAAGAGTATCACAGGAGCTTGCTTATCTTTCAT-CTGATGCCGACTTAG 974
Query 1033 TCATCTTAGAAGGCATGGGCCGTGGAATAGAGACAAACCTTTATGCTCAGTTCAAGTGTG 1092
||||| ||||||||||||| |||||||| |||||||||||||||||||||||||||||||
Sbjct 975 TCATCGTAGAAGGCATGGGTCGTGGAATTGAGACAAACCTTTATGCTCAGTTCAAGTGTG 1034
Query 1093 ATTCCCTCAAGATCGGAATGGTGAAGCATCCAGAAGTAGCACAGTTTCTCGGAGGACGGC 1152
|||| || ||||| |||||||||||||||| ||||||||| |||||||||||||||| |
Sbjct 1035 ATTCTCTGAAGATTGGAATGGTGAAGCATCTTGAAGTAGCAGAGTTTCTCGGAGGACGAC 1094
Query 1153 TTTATGACTGTGTCATCAAATACAATGAAGTTCAGATTTAATTATATTTCA 1203
||||||||||||| ||||||||||||||||||||| |||||||||||||
Sbjct 1095 TTTATGACTGTGTTTTCAAATACAATGAAGTTCAGAGCTAATTATATTTCA 1145
> Ahg931587 jgi|Araly1|931587|scaffold_304044.1
Length=1126
Score = 1164 bits (630), Expect = 0.0
Identities = 959/1115 (86%), Gaps = 33/1115 (3%)
Strand=Plus/Plus
Query 105 GAGATGGTAGCGTTGCCTCTGTTACCGACTCCGATAGAATCGAACTACAGGGCTTGCACC 164
|||||| | |||| |||||||||||||| ||||| ||| | |||||||| || ||||||
Sbjct 18 GAGATGATTCCGTTTCCTCTGTTACCGACGCCGATTGAAACCAACTACAGAGCCTGCACC 77
Query 165 ATTCCTTATCGATTCCCTTCTGATAATCCT--AGAAAGGCTACTCCCACTGAGATCTCCT 222
||||||||| ||||||||| ||||||| || ||||| || ||||| ||||| ||||| |
Sbjct 78 ATTCCTTATAGATTCCCTTGTGATAAT-CTGAAGAAA-GCCACTCCTACTGAAATCTCGT 135
Query 223 GGATCGATC-TCTTCTCCAATTCCATCCCTTCTTTCAAGGAACGTGCAGAGAGCGATA-C 280
||||| | | ||||| | |||||||||||||| |||||| |||| ||||||||||||| |
Sbjct 136 GGATC-AACGTCTTCGCTAATTCCATCCCTTCGTTCAAGAAACGAGCAGAGAGCGATATC 194
Query 281 AACCGTTCCAGATGCTCCTGTTCGAGCTGAAAA-GTTTGCCA-AAAGGTATGC-GGAGAT 337
|| |||||||||||||||| ||||| |||| |||| || |||| ||||| ||| ||
Sbjct 195 -ACTGTTCCAGATGCTCCTGCGAGAGCT-AAAATATTTG-CAGAAAGATATGCGGGA-AT 250
Query 338 TCTTGAGGACCT-GAAGAAAGATCCGGAGAGCCATGGTGGTCCACCGGATTGCATTCTTC 396
|||||| || | |||||||||||| |||||| |||| || ||||| ||| ||||| |||
Sbjct 251 TCTTGAAGA-TTGGAAGAAAGATCCAGAGAGCAATGGCGGGCCACCAGATGGCATTATTC 309
Query 397 TCTGCAGAATTCGTGAGC-TGATACTAAGAGAATTGGGATTTAGGGACATATTTAAGAAA 455
|| || || ||||||||| | |||| ||||| || ||||||| ||||||||| ||||||
Sbjct 310 TCGGCCGAGTTCGTGAGCAT-TTACTCAGAGAGTTAGGATTTAAGGACATATTCAAGAAA 368
Query 456 GTTAAGGATGAGGAGAATGCAAAGGCAATATCACTATTTCCTGAAGTTGTCCGTTTGAGT 515
|| |||||||| |||||||||||||| |||||||||||||||||||||||| || |||||
Sbjct 369 GTCAAGGATGAAGAGAATGCAAAGGCTATATCACTATTTCCTGAAGTTGTCAGTCTGAGT 428
Query 516 GATGCTATT-AACGATGAAGGCAAGCGCATAGAGAATCTGGTCAGAGGGATATTTGCTGG 574
||||||||| || ||||| || || || |||||||| |||| |||||||||||||||||
Sbjct 429 GATGCTATTGAA-GATGACGGAAAACGGTTAGAGAATTTGGTGAGAGGGATATTTGCTGG 487
Query 575 AAACATCTTTGATCTGGGTTCTGCACAGCTTGCTGAGGTTTTCTCAAAAGACGGCATGTC 634
||||||||||||||| ||||||||||||||||||||||||||||||| || ||||| |
Sbjct 488 AAACATCTTTGATCTAGGTTCTGCACAGCTTGCTGAGGTTTTCTCAAGGGATGGCATAT- 546
Query 635 CTTT-TTGGCTAGCTGTCAGAACTTGGTTTCACGACCATGGGTCATTGATGACTTGGATA 693
|||| ||||||| |||||| |||||||| ||||||| |||||||||||||||||||| |
Sbjct 547 CTTTCTTGGCTACCTGTCAAAACTTGGTACCACGACCTTGGGTCATTGATGACTTGGACA 606
Query 694 ACTTTCAAGCTAGATGGCTCAAGAAGCCTTGGAAGAAGGCTGTGATATTTGTCGATAATT 753
|||| ||||| | |||| |||||||||||||||||||||| ||||||||||| |||||||
Sbjct 607 ACTTCCAAGCCAAATGGATCAAGAAGCCTTGGAAGAAGGCAGTGATATTTGTTGATAATT 666
Query 754 CTGGTGCAGACATAATTTTGGGTATCTTGCCCTTTGCTAGAGAAATGCTACGTCTAGGAA 813
||||||||||||||||||||||||| ||||| ||||| ||||| |||| |||| |||||
Sbjct 667 CTGGTGCAGACATAATTTTGGGTATTTTGCCGTTTGCAAGAGAGCTGCTCCGTCGAGGAA 726
Query 814 -TGCAGGTTGTACTGGCTGCTAATGAGCTTCC-GTCTATCAACGATGTAACTTATATCGA 871
| ||||| || ||||||||||| ||||| || | |||||||||||||||| |||| |||
Sbjct 727 CT-CAGGTGGTGCTGGCTGCTAACGAGCTGCCAG-CTATCAACGATGTAACATATACCGA 784
Query 872 GCTTGCAGAGATCT-TGTCTAAGCTGAATGATGAAAATGGACAATTGATGGGTGTTGATA 930
|||| |||| || | |||| || |||| |||| |||||| |||||||| ||||||||||
Sbjct 785 GCTTACAGATAT-TGTGTCGCAGTTGAAGGATGGAAATGGCCAATTGATAGGTGTTGATA 843
Query 931 CCTCAAATCTTCTTATTGCAAATTCAGGGAATGACTTACCGGTTATTGATCT-TGCAAGA 989
| ||||| ||||| |||||||||||||||||||| ||| ||||||| ||||| | |||||
Sbjct 844 CTTCAAAGCTTCTGATTGCAAATTCAGGGAATGATTTAGCGGTTATCGATCTCT-CAAGA 902
Query 990 GTATCACAGGAGGTTGCGTACCTCTCAAGC-GATGCAGACTTGGTCATCTTAGAAGGCAT 1048
|||||||| ||| |||| ||||| || | | ||||||||||| |||||| ||||||||||
Sbjct 903 GTATCACATGAGCTTGCTTACCTTTC-ATCTGATGCAGACTTAGTCATCCTAGAAGGCAT 961
Query 1049 GGGCCGTGGAATAGAGACAAACCTTTATGCTCAGTTCAAGTGTGATTCCCTCAAGATCGG 1108
||| ||||| || ||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 962 GGGTCGTGGGATCGAGACAAACCTTTATGCTCAGTTCAAGTGTGATTCTCTCAAGATCGG 1021
Query 1109 AATGGTGAAGCATCCAGAAGTAGCACAGTTTCTCGGAGGACGGCTTTATGACTGTGTCAT 1168
||||||||||||| |||||||||||||||||||||||||| ||||| ||||||||| |
Sbjct 1022 AATGGTGAAGCATGTTGAAGTAGCACAGTTTCTCGGAGGACGACTTTACGACTGTGTCTT 1081
Query 1169 CAAATACAATGAAGTTCAGATTTAATTATATTTCA 1203
||||||||||||||||||| ||||| | ||||||
Sbjct 1082 TAAATACAATGAAGTTCAGAGTTAAT-ACATTTCA 1115
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 51656552535
Database: halleri_transcript_ahg.fa
Posted date: Sep 25, 2014 2:02 AM
Number of letters in database: 38,939,717
Number of sequences in database: 32,672
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5