BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg337252
Length=1683
Score E
Sequences producing significant alignments: (Bits) Value
AT1G50500.2 | Symbols: HIT1 | Membrane trafficking VPS53 family... 652 0.0
AT1G50970.1 | Symbols: | Membrane trafficking VPS53 family pro... 379 4e-104
> AT1G50500.2 | Symbols: HIT1 | Membrane trafficking VPS53 family
protein | chr1:18707990-18715935 REVERSE LENGTH=2907
Length=2907
Score = 652 bits (353), Expect = 0.0
Identities = 527/609 (87%), Gaps = 19/609 (3%)
Strand=Plus/Plus
Query 945 ACAGATAAAGGTCTCGGGGATAGATGAAAGGGTGATATGTTACATAGTTAATTCTGCTGA 1004
||||||||||||||||| || |||||||||||||||||| ||||||||||||||||||||
Sbjct 1690 ACAGATAAAGGTCTCGGAGAGAGATGAAAGGGTGATATGCTACATAGTTAATTCTGCTGA 1749
Query 1005 GTATTGCTGCAAAACATCTGGTG-ACTTGGCAGAAGAA-GTCTCA-ACAATTATTGACCC 1061
||||||| |||||||||||||| || |||||||| || |||||| | ||||||||||||
Sbjct 1750 GTATTGCCACAAAACATCTGGTGAAC-TGGCAGAA-AACGTCTCAGA-AATTATTGACCC 1806
Query 1062 GCATTACGCAGATGGTGTAGACATGTCAGAGGTCCAGGATAAATTTTCGTG-CGTCATAA 1120
||||| || |||||||||||||||||||||||||||||| ||||||| | ||||||||
Sbjct 1807 ACATTATGCTGATGGTGTAGACATGTCAGAGGTCCAGGATGAATTTTC-AGCCGTCATAA 1865
Query 1121 CGAAAGCATTGATGACACTAGTCCGTGGACTGGAAACTAAATTTGACACAGAAAT-GCAA 1179
||||||||||| | || || |||| |||||| || |||||||||||||||||||| || |
Sbjct 1866 CGAAAGCATTGGTAACGCTGGTCCTTGGACTTGAGACTAAATTTGACACAGAAATGGC-A 1924
Query 1180 GAGATGGCACGTGTTTCATGGGGCACACTTGAGAGTGTTGGTGACCATTCTCAGTACGTA 1239
| |||| |||||||| ||||| ||||||||||||||||||||||||| ||| ||||||
Sbjct 1925 GTGATGACACGTGTTCCATGGAGCACACTTGAGAGTGTTGGTGACCAGTCTGGGTACGTG 1984
Query 1240 AATGGAATATATACAATCCTTAAAAACAGCGTTCCTGTCCTCGGGAAACTTCTAACACCA 1299
||||||||| ||||| |||||| |||| | |||||||||||||||||||||||||||
Sbjct 1985 AATGGAATAAATACAGTCCTTAGCGGCAGCATACCTGTCCTCGGGAAACTTCTAACACCA 2044
Query 1300 GTTTACTTCCAATTCTTCCTGGAAAAGTTGGCATCATCCCTTGGGCTACGGTTCTATGCT 1359
||||||||||| || ||||| || ||| ||||||||||||| || | |||||||||||||
Sbjct 2045 GTTTACTTCCAGTTTTTCCTTGACAAGCTGGCATCATCCCTAGGACCACGGTTCTATGCT 2104
Query 1360 AATATTTTCAGATGCAAGAAAATATCAGAAACTGGAGCTCAGCAGATGTTGCTAGATACG 1419
|||||||||||||||||| || |||| |||||||||||||| |||||||| |||||||||
Sbjct 2105 AATATTTTCAGATGCAAGCAACTATCCGAAACTGGAGCTCAACAGATGTTACTAGATACG 2164
Query 1420 CAAGCCATGGAGAT-GATTCTTCTA-AATATTCCATCTCTTGACAGACAGAC-TGTAAGT 1476
||||| || || | |||||| ||| || ||||| || |||| ||||||||| | || |
Sbjct 2165 CAAGCTGTGAAG-TCGATTCTACTAGAA-ATTCCTTCCCTTGCCAGACAGACCTC-AACT 2221
Query 1477 GCTGCAAGCTATTCGGAATTCGTGAAGC-GTCAAATGAGCAGAGCTGAAGCAGTCCTAAA 1535
||||| |||||||| |||| |||| || || |||||||||||||||||||| |||| ||
Sbjct 2222 GCTGCCAGCTATTCCAAATTTGTGA-GCCGTGAAATGAGCAGAGCTGAAGCACTCCTTAA 2280
Query 1536 GGGTCTTAC 1544
|| || |||
Sbjct 2281 GG-TCATAC 2288
Score = 628 bits (340), Expect = 4e-179
Identities = 695/856 (81%), Gaps = 65/856 (8%)
Strand=Plus/Plus
Query 127 CCATCTGTGAGGGAAGAGTTGGTAACCAACTTTTGCAGCAGGGAGCTTACCTCATATGAA 186
||||||||||||||||||||||||| |||||||||||||||||||| ||||||||||||
Sbjct 824 CCATCTGTGAGGGAAGAGTTGGTAAATAACTTTTGCAGCAGGGAGCTCACCTCATATGAA 883
Query 187 CAAAAGTTTTT--AGGAGCTGAATTGACAAAGTTGGATGAA-ATACAGCGGATATACAAT 243
| ||| ||||| ||||||||||||| ||||||||||| || | | |||||| ||| |
Sbjct 884 C-AAA-TTTTTGAAGGAGCTGAATTGGCAAAGTTGGAT-AAGACAGAGCGGAGATATGCT 940
Query 244 CAGATAAATTGTC-ATATCCGAACAAATCAAGAAATTTGGAAGATTTTTCCTGCTTCGTG 302
|||||| ||| | |||||||||||| |||||||||||||||||||||||||||| ||
Sbjct 941 TGGATAAAGCGTCGA-ATCCGAACAAATGAAGAAATTTGGAAGATTTTTCCTGCTTCTTG 999
Query 303 GCATGTGCCTTAT-GGACTGTGCAT-CAAGATGTGCAAG-ATAACAAGGGACCAAGTAGA 359
||||||||| ||| || || ||||| || | | |||||| | ||||||| | ||||||||
Sbjct 1000 GCATGTGCCGTATAGG-CTATGCATTCA-GTTCTGCAAGCA-AACAAGGAAGCAAGTAGA 1056
Query 360 ATCTATTATGGTTAACATGAAAGAGAAGCCAACTGTAGCGAGATTGCTTCAGGAACTGA- 418
||||||| |||| |||||||||||||||||| |||||||| |||||||| || ||| |
Sbjct 1057 ATCTATTCTGGTCAACATGAAAGAGAAGCCAGTTGTAGCGATATTGCTTCTGGCACT-AC 1115
Query 419 AAAGGAC-GCTAAATTTTGAACTGGAACTTGAAATGAAATTTGGTGGTGGTGTTCCTACT 477
|||| || | | | |||||| |||||||||| |||||||||||||||||||||||||
Sbjct 1116 AAAGTACAG-TGGAGTTTGAAAAAGAACTTGAAAAGAAATTTGGTGGTGGTGTTCCTACT 1174
Query 478 AA-GAATATCGGAGATGACATTGAGGAAACTGTTGACG-GGGAAAATTATAGTCCGAATG 535
|| || ||| | |||||||||||| |||| || | || ||||| || |||||| ||||
Sbjct 1175 AAAGA-TATTGAAGATGACATTGAAGAAATTGGT-ACATGGGAAGATAATAGTCAGAATA 1232
Query 536 TATCCGAAATTCACATGATGTATGAAATGAAGTTTGCTGCTAATCACGACTTGGTGGA-A 594
||||| |||| | || || ||||||| |||||||||||||| ||| || | ||| |
Sbjct 1233 TATCCAAAATCCGCAAGAAATATGAAAAGAAGTTTGCTGCTAGTCAAGAAACCGAGGAGA 1292
Query 595 AC--T---CAA-A--AAACG---GGAATCAAAGATTTATCTGATT-CTGGAGGCAGG-TT 641
| | ||| | ||| | |||| |||||||||||||| || |||||| | || ||
Sbjct 1293 ATGGTTTTCAACAGGAAAAGACCGGAAACAAAGATTTATCTG-TTACTGGAG-CTGGGTT 1350
Query 642 TAACTTCCGTGGAATTATCTCTTCTTGCTTCGAGCCTCATTTAA-TCCCTTATATTGAG- 699
||||||||||||||| |||||||| ||||| || |||||||||| ||| | ||||||||
Sbjct 1351 TAACTTCCGTGGAATGATCTCTTCCTGCTTTGAACCTCATTTAACTCCAT-ATATTGAGT 1409
Query 700 -G-A-G-GAA--C--TTATGCAATG-CCTGGA-AAAAGTTGTTCAGGAGGAAACATGGGA 749
| | | ||| | ||||| | || |||||| |||| |||||||||||||| |||||||
Sbjct 1410 TGGAAGAGAAAACACTTATGGA-TGACCTGGAGAAAA-TTGTTCAGGAGGAATCATGGGA 1467
Query 750 TATTGACGA-GGAAAATCTAAATAATATTTTATCTAGCAGTAGAGAGTTGTTTTTGGCTA 808
| |||| || |||| | ||||||| |||||||||| || | | || | |||| ||
Sbjct 1468 TGTTGAGGATGGAAGC-CAAAATAATGTTTTATCTAGTAGCACACAGCTATTTTCCAATA 1526
Query 809 TAAAGAAAAGCTATGACAA-G-TGTAA-AGCTCTGACTAAGAACCTGACTTTATTCAAGT 865
||||||| |||| ||| || | ||||| | |||| | |||||||| |||||||||||| |
Sbjct 1527 TAAAGAAGAGCT-TGA-AACGCTGTAATA-CTCTCAGTAAGAACCAGACTTTATTCAATT 1583
Query 866 TATTCAAGGTCTTTCAACGAGTTCTACAAG-CCTATGCTACAAAGCTATTTTTTAAGCTC 924
| ||||||||||||||||||||| || ||| |||||||||||||||||||||||||||||
Sbjct 1584 TGTTCAAGGTCTTTCAACGAGTTTTA-AAGGCCTATGCTACAAAGCTATTTTTTAAGCTC 1642
Query 925 TCCACA-GGAGGCACT 939
||| | |||||||||
Sbjct 1643 -CCAAAAGGAGGCACT 1657
> AT1G50970.1 | Symbols: | Membrane trafficking VPS53 family protein
| chr1:18896323-18900268 FORWARD LENGTH=1710
Length=1710
Score = 379 bits (205), Expect = 4e-104
Identities = 304/350 (87%), Gaps = 14/350 (4%)
Strand=Plus/Plus
Query 127 CCATCTGTGAGGGAAGAGTTGGTAACCAACTTTTGCAGCAGGGAGCTTACCTCATATGAA 186
||||||||||||||||||||| ||| ||||||||||||||||||||| ||||||||||||
Sbjct 631 CCATCTGTGAGGGAAGAGTTGATAAACAACTTTTGCAGCAGGGAGCTAACCTCATATGAA 690
Query 187 CAAAAGTTT-T-TAGGAGCTGAATTGACAAA-GTTGGATGAAATACAGCGGATATACAAT 243
| ||| ||| | ||||||||||| ||| ||| ||||||||||||| ||| ||||||||||
Sbjct 691 C-AAA-TTTATGTAGGAGCTGAACTGA-AAACGTTGGATGAAATAGAGCTGATATACAAT 747
Query 244 CAGATAAATTGTCAT-ATCCG-AACAAATCAA-GAAATTTGGAAGATTTTTCCTGCTTCG 300
||| ||| ||||| | ||||| || ||||||| |||| |||| |||||||||||||||
Sbjct 748 CAGCTAAGTTGTC-TAATCCGCAA-AAATCAAGGAAA-GTGGACGATTTTTCCTGCTTCA 804
Query 301 TGGCATGTGCCTTATGGACTGTGCATCAAGATGTGCAAGATAACAAGGGACCAAGTAGAA 360
||||||||||||||| |||| |||||| || || ||| || |||||||| |||||||||
Sbjct 805 TGGCATGTGCCTTATAGACTATGCATCCAGTTGAGCAGGAAAACAAGGGTGCAAGTAGAA 864
Query 361 TCTATTATGGTTAACATGAAAGAGAAGCCA-ACTGTAGCGAGATTGCTTCAGGAACTGAA 419
|||||| |||| ||| ||||||||||| || | ||||| | |||||||| |||||||||
Sbjct 865 TCTATTCTGGTCAACCTGAAAGAGAAGTCAGA-TGTAGAAAAATTGCTTCTGGAACTGAA 923
Query 420 AAGGACGCTAAATTTTGAACTGGAACTTGAAATGAAATTTGGTGGTGGTG 469
|||||||||| | |||||| |||||||||||||||||||||||||||||
Sbjct 924 AAGGACGCTAGAGTTTGAAAGGGAACTTGAAATGAAATTTGGTGGTGGTG 973
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 83182303065
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5