BLASTN 2.2.26+ Reference: Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000), "A greedy algorithm for aligning DNA sequences", J Comput Biol 2000; 7(1-2):203-14. Database: TAIR10_cdna_20110103_representative_gene_model_updated 33,602 sequences; 51,074,197 total letters Query= Ahg359794 Length=1977 Score E Sequences producing significant alignments: (Bits) Value AT4G27010.2 | Symbols: | INVOLVED IN: biological_process unkno... 1637 0.0 AT1G72270.1 | Symbols: | CONTAINS InterPro DOMAIN/s: Ribosome ... 1581 0.0 > AT4G27010.2 | Symbols: | INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 20 plant structures; EXPRESSED DURING: 10 growth stages; CONTAINS InterPro DOMAIN/s: Ribosome 60S biogenesis N-terminal (InterPro:IPR021714); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G72270.1); Has 312 Blast hits to 226 proteins in 103 species: Archae - 0; Bacteria - 2; Metazoa - 129; Fungi - 90; Plants - 83; Viruses - 0; Other Eukaryotes - 8 (source: NCBI BLink). | chr4:13557974-13568296 FORWARD LENGTH=7880 Length=7880 Score = 1637 bits (886), Expect = 0.0 Identities = 1298/1495 (87%), Gaps = 36/1495 (2%) Strand=Plus/Plus Query 1 ATGGCTATTTCTTGGG-ATTTATATGGAACATCATTCTCATTTTTACAGTCCATCGTCTT 59 |||| | ||||||||| ||| ||||||||| ||||||||||||||||||||||| ||| Sbjct 2991 ATGGATGTTTCTTGGGCCTTT-TATGGAACACCATTCTCATTTTTACAGTCCATCACCTT 3049 Query 60 TCTAGAGGAAAACTTCCTCGGAAACCTTTCAAAGCTATCACCTGACTTATTTG-TGAGAG 118 |||||||||||| ||||| |||||||||||||||||||| |||||||| |||| | || Sbjct 3050 TCTAGAGGAAAATTTCCTTGGAAACCTTTCAAAGCTATCGCCTGACTTGTTTGCT-TCAG 3108 Query 119 GTTTAGAACTTACTAGGTCAAGGAATCTCCGTGAAGGAATAGTAGATAGTGAGATTGATT 178 ||| |||| ||||| ||||| ||||||| ||||||||| |||||||||||||||||||| Sbjct 3109 GTTCAGAATTTACTGGGTCAGGGAATCTGTGTGAAGGAACAGTAGATAGTGAGATTGATT 3168 Query 179 TTGCTGACTACTCATCCGTAACTGAAGCAATCAAAAGTAAGATGGAGATTCGTGATATTT 238 || ||| | |||||||||||||||||| ||||| |||||||||||| | ||||||||| Sbjct 3169 TTTCTGGCCACTCATCCGTAACTGAAGAAATCAGAAGTAAGATGGACAATCGTGATATGG 3228 Query 239 ATTCTTCTGCCTTGTCTATGTTCTTCGAGCAGGCTCCTTTTCCAGTATTACTTAACGAAA 298 | ||||| ||||| ||||| ||||| |||||||||||||||| ||||||||||||| || Sbjct 3229 AATCTTCAGCCTTCTCTATATTCTTAAAGCAGGCTCCTTTTCCTGTATTACTTAACGCAA 3288 Query 299 TTATGAGCATGGATATATCTTGCGTGCCAGAGTTTCCAAGATTAGCAGAGCTACTGCTGC 358 ||||||||||||||||||||||| | ||||||||||||||| || |||||||| |||||| Sbjct 3289 TTATGAGCATGGATATATCTTGCTTACCAGAGTTTCCAAGAATATCAGAGCTATTGCTGC 3348 Query 359 ---AAGTTTGGCAACCTAAAAGTGATAGTATTGAAT-CAGATATCCGATTAATACTATTC 414 |||||| || |||||||||| ||||||||| | || |||| | ||||||||| ||| Sbjct 3349 TAAAAGTTTCACAGCCTAAAAGTGGTAGTATTGACTCCA-ATATTCAATTAATACTGTTC 3407 Query 415 TGGCTCTTCCAAATACGATCATCTTACAAGATTCAACCAGCTTCGGTAC-TCTGTCGACT 473 |||||||| ||||||||||||||||||||| ||||||||||| | |||| || || ||| Sbjct 3408 TGGCTCTTTCAAATACGATCATCTTACAAGGTTCAACCAGCTCCAGTACTTC-ATCAACT 3466 Query 474 CTCTGAGATCTGCTTACGTCTCTTGAAGCACTTGTTCTCGCAAATATCAGAA-CGGGGCT 532 |||||||||||||||||||||| ||||| |||||||||| |||||||||||| ||| || Sbjct 3467 CTCTGAGATCTGCTTACGTCTCATGAAGAACTTGTTCTCTCAAATATCAGAACCGGAGC- 3525 Query 533 TTGTTTCTGGACCTTCTTCAGATAAGTTGGTTGCTCCTTTTGCAAAGTGGAAGCATCAAG 592 ||||||||||||| |||||| |||||||| |||| |||||||||||||||||||||||| Sbjct 3526 TTGTTTCTGGACCCTCTTCAAATAAGTTGCCTGCTTCTTTTGCAAAGTGGAAGCATCAAG 3585 Query 593 TGGCCCAGACAGTTCTTTGCCATCCAGTTGTGATGGCACTGTTGGAGAGTCCCTTGGATT 652 ||||| |||||||||||||||| |||||||||||||||||||| |||||||||||||||| Sbjct 3586 TGGCCGAGACAGTTCTTTGCCACCCAGTTGTGATGGCACTGTTAGAGAGTCCCTTGGATT 3645 Query 653 GTGGCACATTACCTCCAGTGCATAATGTTAAGATTTTTTCAGAAACGTCGCTGACGACGA 712 |||||||||||||||||||||| |||||| |||||||||||||||| |||||||| | | Sbjct 3646 GTGGCACATTACCTCCAGTGCAGAATGTTGAGATTTTTTCAGAAACATCGCTGACAATGG 3705 Query 713 GCAGGCTAGTTATTT-GTGAAATAGATCAACACATCCTTGATCTATTGGTTTCTATTTGT 771 |||||||||| |||| ||||||||||||||||||||||| ||||||||||||||| |||| Sbjct 3706 GCAGGCTAGT-ATTTAGTGAAATAGATCAACACATCCTTAATCTATTGGTTTCTACTTGT 3764 Query 772 GAGCACTTTTTGTTTGATGAGAGACAC---ATTGTGCAGGAAGGAG-ATCTCAGGGAAAA 827 || ||||||||||||||||||| || | | | || |||| ||| | |||||| |||| Sbjct 3765 GAACACTTTTTGTTTGATGAGAAACCCCCCAATCTGT-GGAAAGAGGACCTCAGGAAAAA 3823 Query 828 TAAGTCAAGTACGGTTTTTAAGGACTTGGT-GCAAAGGCTTCTTTTGTTATTCAGAGACA 886 |||||||| || | |||||||||||||| | |||||||||| ||| ||| || | || Sbjct 3824 TAAGTCAATTATAGCGTTTAAGGACTTGGTTG-AAAGGCTTCTCTTGGAATTTAGGGTCA 3882 Query 887 AGTTTGAGCTTTGTGTTGGTTCTCAAAGTTACGCTCCGCTTCTCCAACCATCACAATTAA 946 ||||||||||||||||||||||||||||||||| | | |||||||||||| ||||||| | Sbjct 3883 AGTTTGAGCTTTGTGTTGGTTCTCAAAGTTACGTTTCTCTTCTCCAACCAGCACAATTGA 3942 Query 947 TTCATGCGTTGTTGAGATTTATTTCACCTTTCAAAATTTT-AGAGCTAGCCCGTTCCATG 1005 ||||||| |||||||||||||| |||||||| ||| |||| | | | || | ||||||| Sbjct 3943 TTCATGCTTTGTTGAGATTTATATCACCTTTTAAACTTTTTA-ATATTGCTCATTCCATG 4001 Query 1006 CTGAGTAAAATTGATGAGGAAGAATTGGCTAGCCCAAATTCGAGTATGATTATCAGTTTG 1065 ||||||||||||||||||||| ||| | ||||||||||| ||||| ||| |||||||| Sbjct 4002 TTGAGTAAAATTGATGAGGAAGGATTAACAAGCCCAAATTCAAGTATTATTCTCAGTTTG 4061 Query 1066 GGATTGGATATTGCTGGCGGAGCTTTTGAAATGCTTATATCT-TATTCACACCTGCCGGC 1124 ||||||| |||||| || |||||||||||||||||| ||| | |||||||||| |||| | Sbjct 4062 GGATTGGGTATTGCAGGTGGAGCTTTTGAAATGCTTGTAT-TATATTCACACCAGCCGAC 4120 Query 1125 TGCTAAGAGAGGGTTATATGATTTGCTGTGGGAATTAAAAGAAGAGAATTATGACAGTAT 1184 ||||||||||||| ||||||||||||||||||||||| ||||| ||||||||| ||||| Sbjct 4121 TGCTAAGAGAGGGGTATATGATTTGCTGTGGGAATTAGAAGAAAAGAATTATGCCAGTA- 4179 Query 1185 AC-TCATTGAGGAAGTCTATAGCATGGCATGCAGGTTTTCTACCTCTTTTGGTTTGGTTT 1243 || |||||||| |||| |||||||||||||||| ||||||||||||| ||| ||||| || Sbjct 4180 ACATCATTGAGAAAGTATATAGCATGGCATGCAAGTTTTCTACCTCTCTTGATTTGGATT 4239 Query 1244 CGGCAGATACTTGTTTGCTTAAAGTT-G-GGAGCAGCATTTTCAGGGGGAAACATAATCG 1301 | ||||||| |||||||||||||||| | || | | ||||||||||| |||||||||| Sbjct 4240 CAGCAGATATTTGTTTGCTTAAAGTTTGTGGTG--GTATTTTCAGGGGAAAACATAATCA 4297 Query 1302 GCATT--GCAATGTTCATCCATTGACCGTGATAATTTCACAGATTGTTGGGAGAACTCCT 1359 ||| || |||| | || ||| | ||| |||||||| ||||||||| ||||| ||| Sbjct 4298 AAATTATGC--TGTTGACCCGTTGGTCTTGAAAATTTCACTGATTGTTGGAAGAACCCCT 4355 Query 1360 AAAGACTTGATTATCCATTATATTAACCAGCCAAGCATGACCAGAGCCAAGATATTGTTC 1419 |||||||||||||||||| ||||||| | ||||||| ||||||||||||||||| ||| Sbjct 4356 GAAGACTTGATTATCCATTGCATTAACCGGGCAAGCATAACCAGAGCCAAGATATTATTC 4415 Query 1420 TATCTTGTGGAGTCCAGTCCCTTGCATCTGTCAGTCTTTGGACACTCTTTCTTTA 1474 || |||||||||||||||||||||||||||| |||||||||||||| |||||||| Sbjct 4416 TACCTTGTGGAGTCCAGTCCCTTGCATCTGTTAGTCTTTGGACACTTTTTCTTTA 4470 Score = 200 bits (108), Expect = 4e-50 Identities = 189/227 (83%), Gaps = 10/227 (4%) Strand=Plus/Plus Query 1475 ATTATTTGTGGGGTAAAGCTGCCTTGAAAATAAGAGAGGGACTGCGTTTTTCTCAGGATG 1534 ||||||||||||||||||||||||||||||||||||||||||| | | ||||||||| Sbjct 5773 ATTATTTGTGGGGTAAAGCTGCCTTGAAAATAAGAGAGGGACT-C-T----CTCAGGATG 5826 Query 1535 CATATTATGGTGGTGAAGCTGGTTTGGTGGAAAATCTCCAGCAGATTCTT-TTCAAAGAG 1593 ||| | ||| | || ||| || ||| |||||| || ||| |||| |||| || |||||| Sbjct 5827 CATCTGATGTTTGTCAAGTTGATTTAGTGGAAGATGTCCGGCAGGGTCTTATT-AAAGAG 5885 Query 1594 AATCTTTG-GATTGATCCCAAAATATGTGCTCAAACTCTTTTGTATTTTCCCTATCAACG 1652 |||||||| | ||||||||||||| ||||||| |||| |||||| |||||| |||||||| Sbjct 5886 AATCTTTGTG-TTGATCCCAAAATCTGTGCTCTAACTGTTTTGTTTTTTCCTTATCAACG 5944 Query 1653 AACTGCAGAGGTATCGGATAACTCTTACATATCTGATGATCCAGTAA 1699 ||| ||||| |||||| || | |||| ||| |||||||||| ||| Sbjct 5945 TACTACAGAGAAATCGGAGAATTTTTACCTATATGATGATCCAATAA 5991 Score = 169 bits (91), Expect = 1e-40 Identities = 127/144 (88%), Gaps = 3/144 (2%) Strand=Plus/Plus Query 1763 ACTCATTGTCCATGGGATGTATTGAACCTGTAAAATTCGCCAG-TTCAGGCTTGCTTGCA 1821 ||||||||||| |||| | ||||||||||| |||||||||| || ||||||||||||| Sbjct 6023 ACTCATTGTCC-TGGGTTACATTGAACCTGTGGAATTCGCCAGCTT-AGGCTTGCTTGCA 6080 Query 1822 GTTGCGTTAGCGAGCACGTCTTCAGCAGATCTTGGGATGAGAAAATTGGGCTATGAAACT 1881 ||||| || | ||||| ||||||||||||||||||||||||||||||||||||||||||| Sbjct 6081 GTTGCATTTGTGAGCATGTCTTCAGCAGATCTTGGGATGAGAAAATTGGGCTATGAAACT 6140 Query 1882 CTTGGGATATTTGTGCATGCCCTT 1905 ||| |||||| || |||||||| Sbjct 6141 CTTCAAATATTTCTGGATGCCCTT 6164 > AT1G72270.1 | Symbols: | CONTAINS InterPro DOMAIN/s: Ribosome 60S biogenesis N-terminal (InterPro:IPR021714); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G27010.1); Has 772 Blast hits to 657 proteins in 120 species: Archae - 0; Bacteria - 0; Metazoa - 344; Fungi - 94; Plants - 322; Viruses - 0; Other Eukaryotes - 12 (source: NCBI BLink). | chr1:27199733-27211161 REVERSE LENGTH=8577 Length=8577 Score = 1581 bits (856), Expect = 0.0 Identities = 1295/1496 (87%), Gaps = 74/1496 (5%) Strand=Plus/Plus Query 1 ATGGCTA-TTTCTTGGGATTTATATGGAACATCATTCTCATTTTTACAGTCCATCGTCTT 59 ||||| | ||||||||||| | || |||||||||||||||||||||| ||||||| |||| Sbjct 3661 ATGGC-AGTTTCTTGGGATCTCTACGGAACATCATTCTCATTTTTACTGTCCATCATCTT 3719 Query 60 TCTAGAGGAAAACTTCCTCGGAAACCTTTCAAAGCTATCACCTGACTTATTTGTGAGAGG 119 | ||||| |||||||||||||||||||||||||||||||||||||||||||| || |||| Sbjct 3720 TTTAGAGAAAAACTTCCTCGGAAACCTTTCAAAGCTATCACCTGACTTATTTATGCGAGG 3779 Query 120 TTTAGAACTTACTAG-GTCAAGGAATCTCCGTGAAGGAATAGTAGATAGTGAGATTGATT 178 || ||||||||| | ||||||||||||||||||||||| |||||||||||||||||||| Sbjct 3780 GTTGGAACTTACT-GTGTCAAGGAATCTCCGTGAAGGAACAGTAGATAGTGAGATTGATT 3838 Query 179 TTGCTGACTACTCATCCGTAACTGAAGCAATCAAAAGTAAGATGGAGATTCGTGATATTT 238 |||||||| |||||||| ||||||| |||||| ||||||||||| |||| ||||||| Sbjct 3839 TTGCTGACCACTCATCCACAACTGAAAAAATCAAGAGTAAGATGGACATTCCTGATATTG 3898 Query 239 ATTCTTC-TGCCTTGTCTATGTTCTTCGAGCAGGCTCCTTTTCCAGTATTACTTAACGAA 297 | || || ||| || ||| | ||||| |||||| |||||||||||||||||||||| ||| Sbjct 3899 AATC-TCTTGCATTCTCTGTATTCTTAGAGCAGACTCCTTTTCCAGTATTACTTAATGAA 3957 Query 298 ATTATGAGCATGGATATATCTTGCGTGCCAGAGTTTCCAAGATTAGCAGAGCTACTGCTG 357 |||||||||||||||||||||||| | |||||||||||||||||| |||||||||| ||| Sbjct 3958 ATTATGAGCATGGATATATCTTGCTTACCAGAGTTTCCAAGATTAACAGAGCTACTACTG 4017 Query 358 C---AAGTTTGGCAACCTAAAAGTGATAGTATTGAATCAGATATCCGATTAATACTATTC 414 | |||||| ||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 4018 CTTAAAGTTTCGCAACCTAAAAGTGATAGTATTGAATCAGATATCCGATTAATACTATTC 4077 Query 415 TGGCTCTTCCAAATACGATCATCT-TACAAGATTCAACCAGCTTC-GGTACTCTGTCGAC 472 |||||||||||||||||||||| | |||||| |||||||| | | ||||||||||| || Sbjct 4078 TGGCTCTTCCAAATACGATCAT-TGTACAAGGTTCAACCA-CACCCGGTACTCTGTCAAC 4135 Query 473 TCTCTGAGATCTGCTTACGTCTCTTGAAGCACTTGTTCTCGCAAATATCAGAA-CGGGGC 531 |||||||||||||||||||| ||| |||||||||||| ||||||||| || | | | Sbjct 4136 AATCTGAGATCTGCTTACGTCTAATGAGGCACTTGTTCTCTCAAATATCA-AAGCTTGAC 4194 Query 532 TTTGTTTCTGGACCTTCTTCAGATAAGTTGGTTGCTCCTTTTGCAAAGTGGAAGCATCAA 591 ||||||||||||||||| ||||||||||| || | |||||| Sbjct 4195 CTTGTTTCTGGACCTTCTGCAGATAAGTTG--------------AA-G------CATCAA 4233 Query 592 GTGGCCCAGACAGTTCTTTGCCATCCAGTTGTGATGGCACTGTTGGAGAGTCCCT-TGGA 650 ||| ||||||||||||||| ||| |||||||||||||||||||||||||||||| || | Sbjct 4234 GTGCCCCAGACAGTTCTTTCCCACCCAGTTGTGATGGCACTGTTGGAGAGTCCCGCTG-A 4292 Query 651 TTGTGGCACATTACCTCCAGTGCATAATGTTAAGATTTTTTCAGAAACGTCGCTGAC-GA 709 ||||| ||||||||||| |||||| |||||| || ||||||||||||||| |||||| | Sbjct 4293 TTGTGACACATTACCTCGAGTGCAGAATGTTGAGGTTTTTTCAGAAACGTTGCTGACAG- 4351 Query 710 CGAGCAGGCTAGTTATTTGTGAAATAGATCAACACATCCTTGATCTATTGGTTTCTATTT 769 || ||||||||| |||| |||||||||||||||||||||||||||| |||| ||||| || Sbjct 4352 CGGGCAGGCTAGGTATTAGTGAAATAGATCAACACATCCTTGATCTCTTGGCTTCTACTT 4411 Query 770 GTGAGCACTTTTTGTTTGATGAGAGACACATTGTGCAGGAA-GGAGATCTCAGGGAAAAT 828 ||||| ||||||||||||| ||||| || |||| || |||| || || ||||||| | || Sbjct 4412 GTGAGAACTTTTTGTTTGAGGAGAGTCATATTGAGC-GGAAAGGGGACCTCAGGGCAGAT 4470 Query 829 AAGTCAAGTACGGTTTTTAAGGACTTGGT-GCAAAGGCTTCTTTTGTTATTCAGAGACAA 887 ||||||| || || |||||||| |||||| | |||||||||| ||| ||||||||||||| Sbjct 4471 AAGTCAATTATGGCTTTTAAGGTCTTGGTTG-AAAGGCTTCTCTTGGTATTCAGAGACAA 4529 Query 888 GTTTGAGCTTTGTGTTGGTTCTCAAAGTTACGCTCCGCTTCTCCAACCATC-ACAATTAA 946 ||||||||||||||||||||||||||||||||||||||||||||||| ||| |||||||| Sbjct 4530 GTTTGAGCTTTGTGTTGGTTCTCAAAGTTACGCTCCGCTTCTCCAAC-ATCCACAATTAA 4588 Query 947 TTCATGCGTTGTTGAGATTTATTTCACCTTTCAAAATTTTAGAGCTAGCCCGTTCCATGC 1006 |||| || ||||||| |||||| |||||||| ||| ||||| | || || | |||||||| Sbjct 4589 TTCAGGCTTTGTTGAAATTTATATCACCTTTTAAACTTTTATATCTTGCACATTCCATGC 4648 Query 1007 TGAGTAAAA-TTGATGAGGAAGAATTGGCTAGCCCAAATTCGAGTATGATTATCAGTTTG 1065 ||||||||| || | |||||||||||||| ||||| | ||| | | || || | | Sbjct 4649 TGAGTAAAACTT-ACGAGGAAGAATTGGCGAGCCCGA-TTCTC--A-G-TT-TC-G---G 4697 Query 1066 GGATTGGATATTGCTGGCGGAGCTTTTGAAATGCTTATATCT-TATTCACACCTGCCGGC 1124 | ||||||||||||||||||||||||||||||||||||| | ||||||| || ||| || Sbjct 4698 G--TTGGATATTGCTGGCGGAGCTTTTGAAATGCTTATAT-TGTATTCACGCCAGCCTGC 4754 Query 1125 TGCTAAGAGA-GGGTTATATGATTTGCTGTGGGAATTAAAAGA-AGAGAATTATGACAGT 1182 |||||||||| |||| |||||||| |||||||||||| |||| | |||||||||||||| Sbjct 4755 TGCTAAGAGAAGGGTC-TATGATTTTCTGTGGGAATTAGAAGACA-AGAATTATGACAGT 4812 Query 1183 ATACTCATTGAGGAAGTCTATAGCATGGCATGCAGGTTTTCTACCTCTTTTGGTTTGGTT 1242 | | | |||||| |||| |||||| ||||||||||||||||||||||||||||||||| | Sbjct 4813 AGAATTATTGAGCAAGTGTATAGCCTGGCATGCAGGTTTTCTACCTCTTTTGGTTTGGCT 4872 Query 1243 TCGGCAGATACTTGTTTGCTTAAAGTTGGGAGCAGCATTTTCAGGGGGA-AACATAATCG 1301 |||||||||||||||||||||||||| | || |||||||||||||| || ||| || Sbjct 4873 TCGGCAGATACTTGTTTGCTTAAAGTCGTTAGTGGCATTTTCAGGGGGGGAA-ATAGTCA 4931 Query 1302 GCATTGCAATGTTCATCCA-TTGACCGTGATAATTTCACAGATTGTTGGGAGAACTCCTA 1360 |||||| | |||||| ||| ||||| |||||||| ||||||||||||||||||||| ||| Sbjct 4932 GCATTGTAGTGTTCA-CCAGTTGACGGTGATAATGTCACAGATTGTTGGGAGAACTTCTA 4990 Query 1361 AAGAC-TTGATTATCCATTATATTAACCAGCCAAGCATGACCAGAGCCAAGATATTGTTC 1419 ||||| || |||||||||| ||||||||| ||||||||| ||| ||||||||||||||| Sbjct 4991 AAGACCTT-ATTATCCATTGCATTAACCAGGCAAGCATGAGCAGGGCCAAGATATTGTTC 5049 Query 1420 TATCTTGTGGAGTCCAGTCCCTTGCATCTGTCAGTCTTTGGACAC-TCTTTCTTTA 1474 ||||||||||||||||||||||||||||||||||||||||||||| | |||||||| Sbjct 5050 TATCTTGTGGAGTCCAGTCCCTTGCATCTGTCAGTCTTTGGACACAT-TTTCTTTA 5104 Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 97941263295 Database: TAIR10_cdna_20110103_representative_gene_model_updated Posted date: Sep 25, 2014 6:13 PM Number of letters in database: 51,074,197 Number of sequences in database: 33,602 Matrix: blastn matrix 1 -2 Gap Penalties: Existence: 0, Extension: 2.5