BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg470330
Length=1025
Score E
Sequences producing significant alignments: (Bits) Value
AT1G03550.1 | Symbols: | Secretory carrier membrane protein (S... 1548 0.0
AT1G03545.1 | Symbols: | Potential natural antisense gene, loc... 209 4e-53
> AT1G03550.1 | Symbols: | Secretory carrier membrane protein
(SCAMP) family protein | chr1:885623-887956 REVERSE LENGTH=1258
Length=1258
Score = 1548 bits (838), Expect = 0.0
Identities = 890/916 (97%), Gaps = 0/916 (0%)
Strand=Plus/Plus
Query 110 TTGAATAAGTTTCTTTATCTTAAAACAAACGATCTCAAAGAATGGCACGGCACGATCCTA 169
||||||||||||||| ||||||||||||||||||||||||||||||||| ||||||||||
Sbjct 138 TTGAATAAGTTTCTTGATCTTAAAACAAACGATCTCAAAGAATGGCACGACACGATCCTA 197
Query 170 ATCCATTTGCTGATGAAGAGATCAATCCTTTTGCGAATAATACAAGTGTTCCTCCTGCGA 229
|||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
Sbjct 198 ATCCATTTGCTGATGAAGAGATCAATCCTTTTGCGAATCATACAAGTGTTCCTCCTGCGA 257
Query 230 GCAACTCTTATCTCAAGCCACTTCCACCGGAACCTTATGATCGTGGTGCAACCGTTGATA 289
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 258 GCAACTCTTATCTCAAGCCACTTCCACCGGAACCTTATGATCGTGGTGCAACCGTTGATA 317
Query 290 TCCCTTTGGATTCTGGCAATGATCTTCGAGCTAAAGAGATGGAACTTCAGGCTAAGGAGA 349
|||||||||||||||||||||||||||||||||||||||||||||| ||||| |||||||
Sbjct 318 TCCCTTTGGATTCTGGCAATGATCTTCGAGCTAAAGAGATGGAACTCCAGGCCAAGGAGA 377
Query 350 ACGAACTGAAGCGGAAAGAGCAGGAGCTTAAAAGAAGAGAGGATGCAATAGCGCGAACTG 409
||||| ||||||||||||||||||||||||||||||||||||| |||||||| |||||||
Sbjct 378 ACGAATTGAAGCGGAAAGAGCAGGAGCTTAAAAGAAGAGAGGACGCAATAGCACGAACTG 437
Query 410 GAGTTGTCATCGAAGAGAAAAATTGGCCGGAGTTTTTCCCTATAATTCATCATGACATTC 469
|||||||||| |||||||| ||||||||||||||||| ||| ||||||||||||||||||
Sbjct 438 GAGTTGTCATTGAAGAGAAGAATTGGCCGGAGTTTTTTCCTTTAATTCATCATGACATTC 497
Query 470 CAAATGAGATTCCAATTCATTTACAGAAGATCCAATACGTCGCATTCACCACATTGTTAG 529
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 498 CAAATGAGATTCCAATTCATTTACAGAAGATCCAATACGTCGCATTCACCACATTGTTAG 557
Query 530 GTTTAGTAGGATGTCTCTTGTGGAATATTGTGGCAGTAACTGTAGCTTGGATCAAAGGAG 589
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 558 GGTTAGTAGGATGTCTCTTGTGGAATATTGTGGCAGTAACTGTAGCTTGGATCAAAGGAG 617
Query 590 AAGGTCCCACTATATGGCTTCTTTCAATCATCTACTTCATTGCTGGGGTACCTGGAGCTT 649
|||||||||||||||||||||| ||||| || |||||| ||||||||||||||||||| |
Sbjct 618 AAGGTCCCACTATATGGCTTCTCTCAATAATTTACTTCCTTGCTGGGGTACCTGGAGCGT 677
Query 650 ACGTCTTATGGTACCGTCCTCTTTACCGAGCTACCAGAACTGATAGTGCCCTGAAGTTCG 709
|||||||||||||||||||||||||||||||||| ||||||||||||||||||||||| |
Sbjct 678 ACGTCTTATGGTACCGTCCTCTTTACCGAGCTACAAGAACTGATAGTGCCCTGAAGTTTG 737
Query 710 GAGCttttttctttttttACGTGTTTCACATCGCGTTCTGCGGCTTTGCTGCAGTTGCTC 769
||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
Sbjct 738 GAGCTTTTTTCTTTTTTTACGTGTTCCACATCGCGTTCTGCGGCTTTGCTGCAGTTGCTC 797
Query 770 CACCAGTCATCTTCCAAGGAAAGTCTCTCACAGGTTTCTTGCCAGCACTTGAGCTTCTAA 829
||||||||||||||||||||||||||||||||||||||||||||||| | ||||||||||
Sbjct 798 CACCAGTCATCTTCCAAGGAAAGTCTCTCACAGGTTTCTTGCCAGCAATCGAGCTTCTAA 857
Query 830 CTACTAATGCTGCAGTCGGGATAATGTATTTCATTGGTGCTGGGTTTTTCTGCATCGAAA 889
|||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
Sbjct 858 CTACTAATGCTGCAGTCGGGATAATGTATTTCATTGGTGCTGGGTTCTTCTGCATCGAAA 917
Query 890 CACTTCTCAATATCTGGGTCATTCAGCAAGTATATGCATATTTCCGAGGGAGTGGCAAAG 949
|||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
Sbjct 918 CACTTCTCAATATCTGGGTCATTCAGCAAGTATATGCATACTTCCGAGGGAGTGGCAAAG 977
Query 950 CTGCAGAGATGAAGCGTGAAGCTACAAAATCGACCTTGATGCGTGCACTATGAAGAAAAC 1009
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
Sbjct 978 CTGCAGAGATGAAGCGTGAAGCTACAAAATCGACCTTGATGCGTGCACTATGAAGAAATC 1037
Query 1010 ACTCTTCACCAGAAAA 1025
||||||||||||||||
Sbjct 1038 ACTCTTCACCAGAAAA 1053
Score = 176 bits (95), Expect = 4e-43
Identities = 105/110 (95%), Gaps = 0/110 (0%)
Strand=Plus/Plus
Query 1 TAAAATGCCTTCGATTTCTCCGGCGCCATTGATTTAGTTTTCTCTCTCTGACTACAAAGT 60
|||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 1 TAAAATGCCTTCGATTTCTCTGGCGCCATTGATTTAGTTTTCTCTCTCTGACTACAAAGT 60
Query 61 ACCCATTCGTTGAAACCATTTTCTTCCCATTTTCGATTTCTGTCATAATT 110
| ||||||||| |||||| |||||||||||||||||||||||||| ||||
Sbjct 61 ATCCATTCGTTCAAACCAGTTTCTTCCCATTTTCGATTTCTGTCAAAATT 110
> AT1G03545.1 | Symbols: | Potential natural antisense gene, locus
overlaps with AT1G03550 | chr1:885941-887906 FORWARD LENGTH=1223
Length=1223
Score = 209 bits (113), Expect = 4e-53
Identities = 135/145 (93%), Gaps = 4/145 (3%)
Strand=Plus/Minus
Query 591 AGGTCCCACTATATGGCTTCTTTCAATCATCTACTTCATTGCTGGGGTACCTGGAGCTTA 650
||||||||||||||||||||| ||||| || |||||| ||||||||||||||||||| ||
Sbjct 757 AGGTCCCACTATATGGCTTCTCTCAATAATTTACTTCCTTGCTGGGGTACCTGGAGCGTA 698
Query 651 CGTCTTATGGTACCGTCCTCTTTACCGAGCTAC---CAGAACTGATAGTGCCCTGAAGTT 707
||||||||||||||||||||||||||||||| | ||||||||||||||||||||||||
Sbjct 697 CGTCTTATGGTACCGTCCTCTTTACCGAGCT-CTTGCAGAACTGATAGTGCCCTGAAGTT 639
Query 708 CGGAGCttttttctttttttACGTG 732
||||||||||||||||||||||||
Sbjct 638 TGGAGCTTTTTTCTTTTTTTACGTG 614
Score = 209 bits (113), Expect = 4e-53
Identities = 121/125 (97%), Gaps = 0/125 (0%)
Strand=Plus/Minus
Query 407 CTGGAGTTGTCATCGAAGAGAAAAATTGGCCGGAGTTTTTCCCTATAATTCATCATGACA 466
||||||||||||| |||||||| ||||||||||||||||| ||| |||||||||||||||
Sbjct 1103 CTGGAGTTGTCATTGAAGAGAAGAATTGGCCGGAGTTTTTTCCTTTAATTCATCATGACA 1044
Query 467 TTCCAAATGAGATTCCAATTCATTTACAGAAGATCCAATACGTCGCATTCACCACATTGT 526
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1043 TTCCAAATGAGATTCCAATTCATTTACAGAAGATCCAATACGTCGCATTCACCACATTGT 984
Query 527 TAGGT 531
|||||
Sbjct 983 TAGGT 979
Score = 122 bits (66), Expect = 5e-27
Identities = 68/69 (99%), Gaps = 0/69 (0%)
Strand=Plus/Minus
Query 732 GTTTCACATCGCGTTCTGCGGCTTTGCTGCAGTTGCTCCACCAGTCATCTTCCAAGGAAA 791
||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 521 GTTCCACATCGCGTTCTGCGGCTTTGCTGCAGTTGCTCCACCAGTCATCTTCCAAGGAAA 462
Query 792 GTCTCTCAC 800
|||||||||
Sbjct 461 GTCTCTCAC 453
Score = 119 bits (64), Expect = 6e-26
Identities = 66/67 (99%), Gaps = 0/67 (0%)
Strand=Plus/Minus
Query 849 GATAATGTATTTCATTGGTGCTGGGTTTTTCTGCATCGAAACACTTCTCAATATCTGGGT 908
||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||
Sbjct 161 GATAATGTATTTCATTGGTGCTGGGTTCTTCTGCATCGAAACACTTCTCAATATCTGGGT 102
Query 909 CATTCAG 915
|||||||
Sbjct 101 CATTCAG 95
Score = 119 bits (64), Expect = 6e-26
Identities = 66/67 (99%), Gaps = 0/67 (0%)
Strand=Plus/Minus
Query 528 AGGTTTAGTAGGATGTCTCTTGTGGAATATTGTGGCAGTAACTGTAGCTTGGATCAAAGG 587
||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 908 AGGGTTAGTAGGATGTCTCTTGTGGAATATTGTGGCAGTAACTGTAGCTTGGATCAAAGG 849
Query 588 AGAAGGT 594
|||||||
Sbjct 848 AGAAGGT 842
Score = 80.5 bits (43), Expect = 3e-14
Identities = 47/49 (96%), Gaps = 0/49 (0%)
Strand=Plus/Minus
Query 801 AGGTTTCTTGCCAGCACTTGAGCTTCTAACTACTAATGCTGCAGTCGGG 849
|||||||||||||||| | ||||||||||||||||||||||||||||||
Sbjct 304 AGGTTTCTTGCCAGCAATCGAGCTTCTAACTACTAATGCTGCAGTCGGG 256
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 50234147000
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5