BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg472495
Length=1840
Score E
Sequences producing significant alignments: (Bits) Value
AT1G22550.1 | Symbols: | Major facilitator superfamily protein... 2671 0.0
AT1G22570.1 | Symbols: | Major facilitator superfamily protein... 1465 0.0
> AT1G22550.1 | Symbols: | Major facilitator superfamily protein
| chr1:7966533-7968641 REVERSE LENGTH=1859
Length=1859
Score = 2671 bits (1446), Expect = 0.0
Identities = 1703/1827 (93%), Gaps = 18/1827 (1%)
Strand=Plus/Plus
Query 26 TAAatatatatatatatatataGCTTAAAATCAAGTTTCCTCCAACAAG-AAATGGCGAT 84
|||||||| ||| ||| ||||||||||||||| |||||||||| ||||| ||||||||||
Sbjct 38 TAAATATAAATAAATACATATAGCTTAAAATCCAGTTTCCTCCGACAAGAAAATGGCGAT 97
Query 85 AGCTGAAGAAGAAGCTGCACTTATAGAAGATACCGTCAGCGACTCGGTCGACCACCGTGG 144
||| ||||||||||||||||||||||||||| ||||||||||||| || |||||||||||
Sbjct 98 AGCCGAAGAAGAAGCTGCACTTATAGAAGATTCCGTCAGCGACTCCGTTGACCACCGTGG 157
Query 145 ACTTCCCGCCGGAAAATCTTCCACCGGTGGATGGAGATCCGCCTGGTACATTATAGGTGT 204
|||||| |||||||||||||||||||| ||||||||||||||||||||||||||||||||
Sbjct 158 ACTTCCTGCCGGAAAATCTTCCACCGGCGGATGGAGATCCGCCTGGTACATTATAGGTGT 217
Query 205 GGAAGTTGGAGAAAGATTCGCTTACTTTGGGATTGGTTCCAACTTAATTACTTACCTCAC 264
|||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 218 GGAAGTTGGAGAAAGATTTGCTTACTTTGGGATTGGTTCCAACTTAATTACTTACCTCAC 277
Query 265 CGGACCTCTCGGACAATCAACGGCCACCGCCGCCGTGAACGTGAACACATGGTCAGGAAC 324
||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
Sbjct 278 CGGACCTCTCGGACAATCAACAGCCACCGCCGCCGTGAACGTGAACACATGGTCAGGAAC 337
Query 325 CGCTTCGATGCTTCCTGTTTTAGGAGCTTTCATTGCAGATGCTTATCTTGGTCGTTACCG 384
||| ||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 338 CGCCTCGATACTTCCTGTTTTAGGAGCTTTCATTGCAGATGCTTATCTTGGTCGTTACCG 397
Query 385 TACCATTGTTATTGCTTCCCTCATCTACATACTTGGACTAGGACTATTGACCTTATCGTC 444
|||||||||| |||||||||||||||||||||||||||||||||||||||||||||| ||
Sbjct 398 TACCATTGTTGTTGCTTCCCTCATCTACATACTTGGACTAGGACTATTGACCTTATCCTC 457
Query 445 TATCTTAATCCTAATGGGATTATCTGAGCAACG------TAA-CG--ATGCTTCTGCTAA 495
|||||||||||||||||||||||| |||||||| ||| || | |||||||||||
Sbjct 458 TATCTTAATCCTAATGGGATTATCGGAGCAACGTCAACATAACCGCAACGCTTCTGCTAA 517
Query 496 ACCGTTGTTTTGGGTGAATATATTATTCTTTTGTTCTCTGTATTTGGTGGCGATCGGACA 555
|||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 518 ACCGTTTTTTTGGGTGAATATATTATTCTTTTGTTCTCTGTATTTGGTGGCGATCGGACA 577
Query 556 AGGCGGACACAAGCCGTGTGTTCAGGCGTTTGGTGCGGACCAGTTTGATGTGGAAGATCC 615
||||||||||||||| |||||||| || |||||||| |||||||||||||||| ||||||
Sbjct 578 AGGCGGACACAAGCCATGTGTTCAAGCTTTTGGTGCTGACCAGTTTGATGTGGGAGATCC 637
Query 616 AAAGGAGAGAATAAGTAGAGGATCGTTTTTCAATTGGTGGTTCTTGAGTTTATCCGCAGG 675
||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||
Sbjct 638 AAAGGAGAGAATAAGTAGAGGATCGTTTTTCAATTGGTGGTTCTTGAGTTTATCCGCGGG 697
Query 676 AATAACTTTGTCCATTATCGTGGTGGTTTATGTTCAAGACAATGTTAATTGGGCGCTTGG 735
|||||||||||| ||||| ||||||||||| ||||||||||||||||||||||| |||||
Sbjct 698 AATAACTTTGTCGATTATTGTGGTGGTTTACGTTCAAGACAATGTTAATTGGGCTCTTGG 757
Query 736 GTTTGGGATCCCTTGTTTGTTTATGGTTATGGCTCTTGCTCTCTTTTTGCTTGGAAGAAA 795
|||||| |||||||||||||| ||||| ||||||||||||||||| ||| ||||||||||
Sbjct 758 GTTTGGTATCCCTTGTTTGTTCATGGTCATGGCTCTTGCTCTCTTCTTGTTTGGAAGAAA 817
Query 796 AACTTATAGATACCCAAGAATAGATCATGAAGAGAAGAAGAACGCTTTTGCAAGAATCGG 855
|||||||||||| |||||| ||||| || | |||||| ||||||||||| || |||||
Sbjct 818 AACTTATAGATATCCAAGAGGAGATCGCGAGGGGAAGAATAACGCTTTTGCGAGGATCGG 877
Query 856 TCGAGTTTTCTTGGTGGCGTTCAAGAATCGGAAACTGAAGTTAACACATTCGGGTCAGTT 915
| ||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
Sbjct 878 TAGAGTTTTCTTGGTGGCGTTCAAGAATCGGAAACTGAAGTTAACACATTCAGGTCAGTT 937
Query 916 GGAAGATGGTTCGT-CAGAGAAATGTAAAGGACGGCTTGAATTCCTAGCAAAAGCGTCGC 974
||||| |||||||| || ||||||||||||||| ||||| |||||||||||||||| ||
Sbjct 938 GGAAGTTGGTTCGTACA-AGAAATGTAAAGGACAACTTGAGTTCCTAGCAAAAGCGTTGC 996
Query 975 TATCAGGAGAAGGAGGTCTAGAGCCCTGTAATAGCAGGGACGTGGAAGACACGATGGCTT 1034
|| |||||||||||||| ||||||| |||| ||| ||||||||||||||| |||||||||
Sbjct 997 TACCAGGAGAAGGAGGTGTAGAGCCATGTAGTAGTAGGGACGTGGAAGACGCGATGGCTT 1056
Query 1035 TGGTAAGACTTATTCC-ATTGTGGATCACATCGGTGGTGAGCACGATTCCTTACGCTCAA 1093
|||| || |||||||| | | ||||||||||| || ||||||||||||||||||||||||
Sbjct 1057 TGGTTAGGCTTATTCCGA-TATGGATCACATCAGTAGTGAGCACGATTCCTTACGCTCAA 1115
Query 1094 TACGCTACTTTCTTCACAAAGCAAGGCGTTACGGTGGACAGAAAAATCTTGCCGGGTTTG 1153
|| ||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
Sbjct 1116 TATGCTACTTTCTTCACAAAACAAGGCGTTACGGTGGACAGAAAAATCTTGCCGGGTTTC 1175
Query 1154 GAAATCCCTCCGGCTTCTTTTCAGGCGCTAATCGGTTTATCGATTTTCATCTCAGTTCCG 1213
|| |||||||||||||||||||| || || |||||||||||||||||||||||||||||
Sbjct 1176 GAGATCCCTCCGGCTTCTTTTCAAGCACTCATCGGTTTATCGATTTTCATCTCAGTTCCA 1235
Query 1214 ACTTATGAGCGTGTTTTCCTTCCGTTAGCTAGATTGATTACCAAAAAGCCTTCAGGGATC 1273
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1236 ACTTATGAGCGTGTTTTCCTTCCGTTAGCTAGATTGATTACCAAAAAGCCTTCAGGGATC 1295
Query 1274 ACAATGCTACAGAGAATAGGAGCTGGAATGGTGCTCTCTAGCTTAAATATGGTGGTTGCA 1333
||||||||||| |||||||||||||||||||||||||| |||||||||||||||||||||
Sbjct 1296 ACAATGCTACAAAGAATAGGAGCTGGAATGGTGCTCTCCAGCTTAAATATGGTGGTTGCA 1355
Query 1334 GCATTGGTAGAAATGAAACGGCTCGAGACGGCTAAGGAACATGGGCTTGTGGATAGACCG 1393
|| || ||||||||||||||||||||||||||||| ||||||||||||||||||||||||
Sbjct 1356 GCGTTAGTAGAAATGAAACGGCTCGAGACGGCTAAAGAACATGGGCTTGTGGATAGACCG 1415
Query 1394 GACGCAACCATCCCAATGTCCATATGGTGGTTCGTTCCTCAGTATTTACTGCTCGGGATG 1453
|||||||||||||||||||||||||||||||||||||||||||||||||| || ||||||
Sbjct 1416 GACGCAACCATCCCAATGTCCATATGGTGGTTCGTTCCTCAGTATTTACTACTTGGGATG 1475
Query 1454 ATCGATGTATTCTCACTAGTAGGTACTCAAGAATTCTTCTACGACCAAGTCCCCACGGAG 1513
|||||||||||||||||||| || || |||||||||||||||||||| ||||||||||||
Sbjct 1476 ATCGATGTATTCTCACTAGTGGGGACACAAGAATTCTTCTACGACCAGGTCCCCACGGAG 1535
Query 1514 CTGAGGAGTATTGGTCTCGCACTTTCTTTGAGCGCAATGGGTCTTGCCAGCTTCTTGAGT 1573
|| |||||||||||||| |||||||||||||| || ||||||||||| |||||||||||
Sbjct 1536 CTAAGGAGTATTGGTCTTGCACTTTCTTTGAGTGCGATGGGTCTTGCGAGCTTCTTGAGC 1595
Query 1574 GGTTTTCTAATCACTGTGATTAATTGGGCGACCGGGAAAAATGGTGGTGGTAGCTGGTTC 1633
||||||||||||||||||||||||||||| |||||||||||||| || | ||||||||||
Sbjct 1596 GGTTTTCTAATCACTGTGATTAATTGGGCTACCGGGAAAAATGGCGGCGATAGCTGGTTC 1655
Query 1634 AACACTAATCTGAACCGAGCCCATGTCGATTATTTTTACTGGTTGCTTGCCGCTTTCACC 1693
|| ||||| |||||| |||||||||||||||||||||||||||||||||| ||||||||
Sbjct 1656 AATACTAACCTGAACAGAGCCCATGTCGATTATTTTTACTGGTTGCTTGCTGCTTTCACT 1715
Query 1694 GCCATTGGGTTCTTGGCGTTTTTGTTCTTCTCCAGGCTGTATGTGTATCGCCGGGTAGAT 1753
|||||||||||||||||||||||||| || ||||||||||||||||||||||| ||||||
Sbjct 1716 GCCATTGGGTTCTTGGCGTTTTTGTTATTATCCAGGCTGTATGTGTATCGCCGTGTAGAT 1775
Query 1754 CAAATCTAAGAATATAAAATTCAACGTGTATCTACTTTCATATCATAAAATTGGTGTTAT 1813
||| |||||||| | |||| ||||||||||||||||||||||||||| ||||| | ||
Sbjct 1776 CAAGTCTAAGAAAAAAAAAGTCAACGTGTATCTACTTTCATATCATAT--TTGGT-T-AT 1831
Query 1814 TCGTAACGTGGTTAGAAACTCTTTAAC 1840
|| ||||||||||||||||||||||||
Sbjct 1832 TCATAACGTGGTTAGAAACTCTTTAAC 1858
> AT1G22570.1 | Symbols: | Major facilitator superfamily protein
| chr1:7976620-7978573 REVERSE LENGTH=1698
Length=1698
Score = 1465 bits (793), Expect = 0.0
Identities = 1438/1736 (83%), Gaps = 98/1736 (6%)
Strand=Plus/Plus
Query 82 GATAGCTGAAGAAGAAGCTGCACT-TATAGAAGA-TACCGTCAGCGACTCGGTCGACCAC 139
|||| | || ||||||| |||||| | ||||||| || ||| ||||| || |||||||||
Sbjct 6 GATACCAGAGGAAGAAGTTGCACTCT-TAGAAGATTA-CGTTAGCGATTCCGTCGACCAC 63
Query 140 CGTGGACTTCCCGCCGGAAAATCTTCCACCGGTGGATGGAGATCCGCCTGGTACATTATA 199
|||||| ||||||||||||||||||||||||| ||||||||||| ||||||| |||||||
Sbjct 64 CGTGGATTTCCCGCCGGAAAATCTTCCACCGGCGGATGGAGATCGGCCTGGTTCATTATA 123
Query 200 GGTGTGGAAGTTGGAGAAAGATTCGCTTACTTTGGGATTGGTTCCAACTTAATTACTTAC 259
||||||||||||| ||| || || |||||||||||||||| | ||| || |||||||||
Sbjct 124 GGTGTGGAAGTTGCAGAGAGGTTTGCTTACTTTGGGATTGCCTGCAATTTGATTACTTAC 183
Query 260 CTCACCGGACCTCTCGGACAATCAACGGCCACCGCCGCCGTGAACGTGAACACATGGTCA 319
||||||||||||||||||||||| ||||| | || ||||||||||||||||| |||||
Sbjct 184 CTCACCGGACCTCTCGGACAATCGACGGCGAAAGCTGCCGTGAACGTGAACACGTGGTCG 243
Query 320 GGAACCGCTTCGATGCTTCCTGTTTTAGGAGCTTTCATTGCAGATGCTTATCTTGGTCGT 379
||||| || || || |||||| |||||||||||||| | || || |||||||||||||||
Sbjct 244 GGAACTGCCTCTATACTTCCTATTTTAGGAGCTTTCGTAGCTGACGCTTATCTTGGTCGT 303
Query 380 TACCGTACCATTGTTATTGCTTCCCTCATCTACATACTTGGA-CTAGGACTATTGACCTT 438
|||||||| ||||| | |||||||||||||||||| |||| |||||||||||||| ||
Sbjct 304 TACCGTACTATTGTCGTAGCTTCCCTCATCTACATA-TTGGGGCTAGGACTATTGACGTT 362
Query 439 ATC-GTCTATCTT-AATCCTAATGGGATTATCTGAGCAACGTAACGATGCTTCTGCTAAA 496
||| | || |||| |||| |||||||||||||| ||||||||||||| ||||||||||||
Sbjct 363 ATCAG-CT-TCTTTAATCATAATGGGATTATCTAAGCAACGTAACGACGCTTCTGCTAAA 420
Query 497 CCGT-TGTTTTGGGTGAATATA-TTATTCTTTTGTTCTCTGTATTTGGTGGCGATCGGAC 554
|| | | ||| |||| |||| | || ||||| ||||||||||||||||||||||||||||
Sbjct 421 CCATCTATTT-GGGTTAATACACTT-TTCTTCTGTTCTCTGTATTTGGTGGCGATCGGAC 478
Query 555 AAGGCGGACACAAGCCGTGTGTTCAGGCGTTTGGTGCGGACCAGTTTGATG-TGGAAGAT 613
|||| |||||||| ||||||||||| ||||||||||||||||||||||||| || |||||
Sbjct 479 AAGGTGGACACAAACCGTGTGTTCAAGCGTTTGGTGCGGACCAGTTTGATGCTG-AAGAT 537
Query 614 CCAAAGGAGAG-AATAA-GTAGAGGATCGTTTTTCAATTGGTGGTTCTTGAGTTTATCCG 671
|| |||||| | |||| | ||||| |||||||||||||||||||||||||||||||| |
Sbjct 538 CCGAAGGAG-GTAATAGCG-AGAGGGTCGTTTTTCAATTGGTGGTTCTTGAGTTTATCTG 595
Query 672 CAGGAATAA-CTTTGTCCATTATCGTGGTGGTTTATGTTCAAGACAATGTTAATTGGGCG 730
| ||||||| | | || ||||| ||||| | |||||||||||| |||||||||||||||
Sbjct 596 CGGGAATAAGCATA-TCGATTATTGTGGTCGCTTATGTTCAAGAAAATGTTAATTGGGCG 654
Query 731 CTTGGGTTTGGGATCCCTTGTTTGTTTATGGTTATGGCTCTTGCTCTCTTTTTGCTTGGA 790
||||||||||||| |||||||||||||||||||||||||||||| |||| ||||| ||
Sbjct 655 TTTGGGTTTGGGATTCCTTGTTTGTTTATGGTTATGGCTCTTGCTATCTTCTTGCTCGGG 714
Query 791 AGAAAAACT-TATAGATACCCAA-GAATAGATCATGAAGAG---AAGA--AG-AACGCTT 842
|| || | | ||||||||||||| | | ||||||| ||| || | || ||| |||
Sbjct 715 AGGAAGA-TATATAGATACCCAAAGGG-ACATCATGAGGAGGTTAATAGTAGTAACACTT 772
Query 843 TTGCAAGAATCGGTCGAGTTTTC-TTGGTGGCGTTCAAGAATCGGAAACTGAAGTTA-A- 899
|||||||||||||| ||||||| || || ||||||||||||||||||||| |||| |
Sbjct 773 TTGCAAGAATCGGTAGAGTTTTTGTTATTG-CGTTCAAGAATCGGAAACTGAGGTTAGAA 831
Query 900 CA--C-A-TTCGG----G--TCA-G-T-T---GGAAGATGGTTC-GTCAGAGAAATGTAA 942
|| | | || || | ||| | | | ||| |||||| | ||| |||||| ||||
Sbjct 832 CATTCTAGTTTGGAATTGGATCAAGGTCTATTGGAGGATGGT-CAGTCCGAGAAACGTAA 890
Query 943 AGGA-CGGCTTGAATT-CCTAGCAAAAGCG-TCGCTATCAGGAGAAGGAGGTCTAGAGCC 999
|| | ||||| |||| |||||||||||| | | | ||| ||||||| | | | |||||
Sbjct 891 AG-ATCGGCTC-AATTTCCTAGCAAAAGCAAT-GATCTCAAGAGAAGGTG-T-T-GAGCC 944
Query 1000 CTGTAATAGCAGGGACGTGGAAGACACGATGGCTTTGGTAAGACTTATTCC-ATTGTGGA 1058
|||| | | ||||||||||| ||| ||| |||||||||||| |||||||| || ||||
Sbjct 945 ATGTAGTGGTAGGGACGTGGATGACGCGAAGGCTTTGGTAAGGCTTATTCCGATA-TGGA 1003
Query 1059 TCACAT-CGGTGGTGAGCACGATTCCTTACGCTCAATACGCTA-CTTTCTTCACAAAGCA 1116
|||||| || |||||||||||||||| |||||||| ||| || |||||||||| |||||
Sbjct 1004 TCACATACG-TGGTGAGCACGATTCCGTACGCTCAGTACA-TAACTTTCTTCACGAAGCA 1061
Query 1117 AGGCGTTACGGTGGACAGAAAAATCTTGCCGGGTTTGGAAATCCCTCCGGCTTCTTT-TC 1175
||| || ||||||||||||| |||||| ||||| | ||||| || |||||||||| |
Sbjct 1062 AGGGGTCACGGTGGACAGAAGAATCTTACCGGGAGTAGAAATTCCAGCGGCTTCTTTATT 1121
Query 1176 AGGCGCTAATCGGTTTATCGATTTTCATCTCAGTTCCGACT-TATGAGCGTGT-TTTCCT 1233
| | | | ||| |||| ||| |||||||||| || || ||||||||||| |||| |
Sbjct 1122 -GTCATTCGTTGGTGTATCTATTCTCATCTCAGTGCCC-CTATATGAGCGTGTATTTC-T 1178
Query 1234 TCCGTTAGCTAG-ATTGATTACCAAAAAGCCTTCAGGGATCACAATGCTACAGAGAATAG 1292
||| ||||||| | ||||||||||||||||| |||||||||||||| || || ||||
Sbjct 1179 CCCGATAGCTAGGAA-GATTACCAAAAAGCCTTTTGGGATCACAATGCTCCAAAGGATAG 1237
Query 1293 GAGCTGGAATGGTGCTCTCTAG-CTTAAATATGGTGGTTGCAGCATTGGTAGAAATGAAA 1351
|||||||||||||||| || || ||| |||||| || | || ||||| |||||| | |||
Sbjct 1238 GAGCTGGAATGGTGCTTTC-AGTCTTCAATATGATGCTCGCTGCATTAGTAGAA-TCAAA 1295
Query 1352 -CGGCTCGAGA-CGGCTAAGGAACATGGGCTTGTGGATA-GACCGGACGCAACCATCCCA 1408
||||| | | || ||| |||||| ||||| ||||| | | ||||||| ||| | |||
Sbjct 1296 ACGGCTAAAAATCG-CTAGGGAACACGGGCTAGTGGACAAG-CCGGACGTGACCGTTCCA 1353
Query 1409 ATGTCCATATGGTGGTTCGTTCCTCAGTATTTACTGCTCGGGATGATCGATGTATTCTC- 1467
|||||||||||||||||||| ||||||||||| || ||||||||||||||| | |||||
Sbjct 1354 ATGTCCATATGGTGGTTCGTCCCTCAGTATTTGCTACTCGGGATGATCGATCTGTTCTCT 1413
Query 1468 ACTAGTAGGTACTCAAGAATTCTTCTACGACCAAGTCCCCACGGAGCTGAGGAGTATTGG 1527
| | || || || |||||||||||||||||||||||||| |||||||| ||||| |||||
Sbjct 1414 A-TGGTGGGAACACAAGAATTCTTCTACGACCAAGTCCCAACGGAGCTAAGGAGCATTGG 1472
Query 1528 TCTCGCACTTTCTTTGAGCGCAATGGGTCTTGCCAGCTTCTTGAGTGGTTTTCTAATCAC 1587
|||| | |||||| |||| || ||||||||| | |||||||||||||||||||| ||||
Sbjct 1473 TCTCTCGCTTTCTCTGAGTGCGATGGGTCTTTCAAGCTTCTTGAGTGGTTTTCTCATCAG 1532
Query 1588 TGTGATTAATTGGGCGACCGGGAAAAATGGTGGTGGTAGCTGGTTCAACACTAATCT-GA 1646
| ||||| ||||||| || ||||||| | || |||||||||| |||| || ||
Sbjct 1533 TTTGATTGATTGGGCTAC-GGGAAAA--GACGG------CTGGTTCAACTCTAA-CTTGA 1582
Query 1647 ACCGAGCCCATGTCGATTATTTTTACTGGTTGCTTGCCGCTTTCACCGCCATTGGGTTCT 1706
||||||||||||| ||||| ||||| |||||||| || |||||||| ||||||| ||||
Sbjct 1583 ACCGAGCCCATGTGGATTACTTTTATTGGTTGCTCGCTGCTTTCACGGCCATTGCTTTCT 1642
Query 1707 TGGCGTTTTTGTTCTTCTCCAGGCTGTATGTGTATCGCCGGGTAGATCAAATCTAA 1762
| ||||| |||||| |||||| | ||||||||||||| ||| |||| ||| |||||
Sbjct 1643 TTGCGTTCTTGTTCATCTCCAAGATGTATGTGTATCGTCGGCTAGACCAAGTCTAA 1698
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 91063788630
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5