BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg473728
Length=995
Score E
Sequences producing significant alignments: (Bits) Value
AT1G37150.2 | Symbols: HCS2 | holocarboxylase synthetase 2 | ch... 1563 0.0
AT2G25710.1 | Symbols: HCS1 | holocarboxylase synthase 1 | chr2... 1264 0.0
> AT1G37150.2 | Symbols: HCS2 | holocarboxylase synthetase 2 |
chr1:14174981-14177302 REVERSE LENGTH=1129
Length=1129
Score = 1563 bits (846), Expect = 0.0
Identities = 950/1000 (95%), Gaps = 7/1000 (1%)
Strand=Plus/Plus
Query 1 ATGGACATTGATGCTTCGTGTAGCTTGGTATTATGTGGTAAATCTTCTGTAGAAACTGAT 60
||||| ||||||||||||||||||||||| |||| ||||||||| |||||||||||||||
Sbjct 92 ATGGATATTGATGCTTCGTGTAGCTTGGTTTTATATGGTAAATCCTCTGTAGAAACTGAT 151
Query 61 ACAGCAACACGTATGAAGAACAATAACGTTCTTAAACTCCCAGATAATACCAAAGTTTCA 120
|||||||||||| ||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 152 ACAGCAACACGTTTGAAGAACAATAACGTTCTTAAACTCCCAGATAATTCCAAAGTTTCA 211
Query 121 ATCTTTTTACAATCTGAGATCAAGAAT-TTGGTCAGAGATGATGA---TTCCTTCAATCT 176
||||||||||||||||||||||||||| || |||||||||||||| ||||||||||||
Sbjct 212 ATCTTTTTACAATCTGAGATCAAGAATCTT-GTCAGAGATGATGATTCTTCCTTCAATCT 270
Query 177 TTCACTATTCATGAACTCGATTTCGACTCATCGGTTTGGTCGGTTTCTCATTTGGTCTCC 236
||| | |||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 271 TTCGTTGTTCATGAACTCGATTTCGACTCATCGGTTTGGTCGGTTTCTCATTTGGTCTCC 330
Query 237 ATTCTTGTCTTCCACTCACGATGTTGTTTCTCATAACTTCTCTGAGATTCCAGTTGGGTC 296
|| |||||||||||||||||||||||||||||||||||||||||||||||||||||| ||
Sbjct 331 ATACTTGTCTTCCACTCACGATGTTGTTTCTCATAACTTCTCTGAGATTCCAGTTGGTTC 390
Query 297 AGTGTGTGTCACAGATATTCAGTTTAAAGGTCGAGGCAGAACAAAGAATGTTTGGGAATC 356
||| |||||| | ||||| || |||||||||||||||||||||||||||||||||||||
Sbjct 391 AGTTTGTGTCTCTGATATACAACTTAAAGGTCGAGGCAGAACAAAGAATGTTTGGGAATC 450
Query 357 TCCAAAAGGTTGTCTTATGTATTCGTTTACCGTAGAAATGGAAGATGGTCGAGTAGTGCC 416
|||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||
Sbjct 451 TCCAAAAGGTTGTCTTATGTATTCGTTTACATTAGAAATGGAAGATGGTCGAGTAGTGCC 510
Query 417 TTTGATACAGTATGTAGTGTCTCTTGCTGTTACTGAGGCAGTGAAAGATGTTTGTGACAA 476
||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
Sbjct 511 TTTGATACAGTATGTGGTGTCTCTTGCTGTTACTGAGGCAGTGAAAGATGTTTGTGACAA 570
Query 477 GAAGGGTTTGTCGTATATTGATGTTAAAATAAAGTGGCCAAATGATCTATATTTGAATGG 536
||||||||||||||||| ||||||||||||||||||||||||||||||||| ||||||||
Sbjct 571 GAAGGGTTTGTCGTATAATGATGTTAAAATAAAGTGGCCAAATGATCTATACTTGAATGG 630
Query 537 CCTTAAGGTTGGAGGCATTTTGTGTACTTCCACATACGGATCAAGGAAGTTCCATGTCAG 596
||||||| ||||||||||||||||||| || ||||| ||||||||||||||| ||||||
Sbjct 631 CCTTAAGATTGGAGGCATTTTGTGTACCTCTACATATAGATCAAGGAAGTTCCTTGTCAG 690
Query 597 TGTTGGTGTGGGATTGAATGTGGACAACGAGAAACCGACCACGTGCTTAAATGCAGTACT 656
||||||||||||||||||||||||||||||| |||||||||||||||||||||| |||||
Sbjct 691 TGTTGGTGTGGGATTGAATGTGGACAACGAGCAACCGACCACGTGCTTAAATGCGGTACT 750
Query 657 AAAAGACAATTGTCCTGCATCAAATCTACTTAAAAGAGAAGAAATTCTTGGTGCCTTCTT 716
||||||| ||| ||| ||||||| |||||||||||||||||||||||||||||||||||
Sbjct 751 AAAAGACGTTTGCCCTCCATCAAACCTACTTAAAAGAGAAGAAATTCTTGGTGCCTTCTT 810
Query 717 TCATAAATTCGAAACA-TTCTTTGATCTATTCATGGAACAAGGTTTTAAATCTCTCGAGG 775
| | || || |||| | |||||||||||||||||||||||||||||||||||||| ||||
Sbjct 811 TAAGAAGTTTGAAA-ATTTCTTTGATCTATTCATGGAACAAGGTTTTAAATCTCTTGAGG 869
Query 776 AGCTTTACTATAGGACATGGCTTCACAGCGGACAAAGAGTGATTGTTGAAGaaaaaaaCG 835
|||||||||||||||||||||||||||| |||||||||||||||| ||||||||||||||
Sbjct 870 AGCTTTACTATAGGACATGGCTTCACAGTGGACAAAGAGTGATTGCTGAAGAAAAAAACG 929
Query 836 AGGACCAAGTTGTTCAAAATGTTGTGACTATCCAGGGTTTGACTTCTTCGGGATATTTGC 895
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 930 AGGACCAAGTTGTTCAAAATGTTGTGACTATCCAGGGTTTGACTTCTTCGGGATATTTGC 989
Query 896 TAGCTATTGGAGATGACAATGTAATGTATGAGCTTCATCCTGATGGCAATAGTTTTGATT 955
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 990 TAGCTATTGGAGATGACAATGTAATGTATGAGCTTCATCCTGATGGCAATAGTTTTGATT 1049
Query 956 TTTTCAAAGGGCTGGTCCGAAGAAAACTATGAACCAAAGA 995
|||||||||||||||||||||||||||||||||||| |||
Sbjct 1050 TTTTCAAAGGGCTGGTCCGAAGAAAACTATGAACCAGAGA 1089
> AT2G25710.1 | Symbols: HCS1 | holocarboxylase synthase 1 | chr2:10952658-10955377
FORWARD LENGTH=1481
Length=1481
Score = 1264 bits (684), Expect = 0.0
Identities = 903/1007 (90%), Gaps = 21/1007 (2%)
Strand=Plus/Plus
Query 1 ATGGACATTGATGCTTCGTGTAGCTTGGTATTATGTGGTAAATCTTCTGTAGAAACTGAT 60
||||| | |||||| || |||||||| || || ||||| ||||| ||||| ||||||||
Sbjct 176 ATGGAGAGTGATGCATCTTGTAGCTTAGTTTTGTGTGGGAAATCCTCTGTGGAAACTGAG 235
Query 61 ACAGCAACA-CGTATGAAGAACAATAACGT-TCTTAAACTCCCAGATAATACCAAAGTTT 118
||||| | || |||||||||| ||| | |||||||||||||||||||||||||||||
Sbjct 236 GTAGCAA-AGGGTTTGAAGAACAAGAAC-TCTCTTAAACTCCCAGATAATACCAAAGTTT 293
Query 119 CAATCTTTTTACAATCTGA-GATCAAGAATTTGG-T-CAGA-GATGATGATTCCTTCAAT 174
|| || ||||| ||||||| | | |||||||||| | ||| |||||| |||||||||||
Sbjct 294 CACTCATTTTAGAATCTGAGGCT-AAGAATTTGGTTAAAGATGATGATAATTCCTTCAAT 352
Query 175 CTTTCACTATTCATGAACTCGATT-TCGACTCATCGGTTTGGTCGGTTTCTCATTTGGTC 233
|||||||| || |||||||||||| | |||||||||||||| |||||||||||||||||
Sbjct 353 CTTTCACTCTTTATGAACTCGATTAT-AACTCATCGGTTTGGACGGTTTCTCATTTGGTC 411
Query 234 TCC-ATTCTTGTCTTCCACTCACGATGTTGTTTCTCATAACTTCTCTGAGATTCCAGTTG 292
||| | |||||||||||||||||||||||||||||||||||||||||| |||||||||
Sbjct 412 TCCGCGT-TTGTCTTCCACTCACGATGTTGTTTCTCATAACTTCTCTGAGCTTCCAGTTG 470
Query 293 GGTCAGTGTGTGTCACAGATATTCAGTTTAAAGGTCGAGGCAGAACAAAGAATGTTTGGG 352
| ||||| |||||||| ||||| |||||||| ||||||||||||||||||||||| ||||
Sbjct 471 GTTCAGTTTGTGTCACTGATATCCAGTTTAAGGGTCGAGGCAGAACAAAGAATGTATGGG 530
Query 353 AATCTCCAAAAGGTTGTCTTATGTATTCGTTTAC-CGTAGAAATGGAAGATGGTCGAGTA 411
|||||||||| ||||||||||||||||| ||||| | ||||||||||||||||||||||
Sbjct 531 AATCTCCAAAGGGTTGTCTTATGTATTCTTTTACTC-TAGAAATGGAAGATGGTCGAGTC 589
Query 412 GTGCCTTTGATACAGTATGTAGTGTCTCTTGCTGTTACTGAGGCAGTGAAAGATGTTTGT 471
|||||||||||||||||||| || ||||||||||| ||||||||||||||||||||||||
Sbjct 590 GTGCCTTTGATACAGTATGTGGTATCTCTTGCTGTAACTGAGGCAGTGAAAGATGTTTGT 649
Query 472 GACAAGAAGGGTTTGTCGTATATTGATGTTAAAATAAAGTGGCCAAATGATCTATATTTG 531
||||||||||||||| |||| |||||||||||||||||||||||||||||||| || ||
Sbjct 650 GACAAGAAGGGTTTGCCGTACATTGATGTTAAAATAAAGTGGCCAAATGATCTGTACGTG 709
Query 532 AATGGCCTTAAGGTTGGAGGCATTTTGTGTACTTCCACATACGGATCAAGGAAGTTCCAT 591
||||| |||||||||||||||||||||||||| || || || |||||| ||||||| ||
Sbjct 710 AATGGTCTTAAGGTTGGAGGCATTTTGTGTACCTCTACGTATAGATCAAAGAAGTTCAAT 769
Query 592 GTCAGTGTTGGTGTGGGATTGAATGTGGACAACGAGAAACCGACCACGTGCTTAAATGCA 651
||||||||||||||||| |||||||||||||||| | |||||||||||||||||||||||
Sbjct 770 GTCAGTGTTGGTGTGGGGTTGAATGTGGACAACGGGCAACCGACCACGTGCTTAAATGCA 829
Query 652 GTACTAAAAGACAATTG-TCCTGCATCAAATCTACTTAAAAGAGAAGAAATTCTTGGTGC 710
|||||||||| | || | ||||| |||||| |||||||||||||| ||||| ||||||||
Sbjct 830 GTACTAAAAGGC-ATGGCTCCTGAATCAAACCTACTTAAAAGAGAGGAAATCCTTGGTGC 888
Query 711 CTTCTTTCATAAATTCGAAACATTCTTTGATCTATTCATGGAACAAGGTTTTAAATCTCT 770
||||||||||||||| |||| |||||| |||||||||||||| |||||||||||||||||
Sbjct 889 CTTCTTTCATAAATTTGAAAAATTCTTCGATCTATTCATGGACCAAGGTTTTAAATCTCT 948
Query 771 CGAGGAGCTTTACTATAGGACATGGCTTCACAGCGGA-CAAAGAGTGATTGTTGAAGa-a 828
||| ||||||||||| ||||||||||| |||||| || |||||||||||||||||||| |
Sbjct 949 CGAAGAGCTTTACTACAGGACATGGCTACACAGC-GAGCAAAGAGTGATTGTTGAAGACA 1007
Query 829 aaaaaCGAGGACCAAGTTGTTCAAAATGTTGTGACTATCCAGGGTTTGACTTCTTCGGGA 888
|| | |||||||||||||||||||||||||||||||||||||||||||||||||| |||
Sbjct 1008 AAGTA-GAGGACCAAGTTGTTCAAAATGTTGTGACTATCCAGGGTTTGACTTCTTCTGGA 1066
Query 889 TATTTGCTAGCTATTGGAGATGACAATGTAATGTATGAGCTTCATCCTGATGGCAATAGT 948
|||||||| ||| |||||||||||||| ||||||||||||||| |||||||||||||||
Sbjct 1067 TATTTGCTGGCTGTTGGAGATGACAATCAAATGTATGAGCTTCACCCTGATGGCAATAGT 1126
Query 949 TTTGATTTTTTCAAAGGGCTGGTCCGAAGAAAACTATGAACCAAAGA 995
||||| ||||||||||| ||||| ||||||||| ||||| |||||||
Sbjct 1127 TTTGACTTTTTCAAAGGTCTGGTTCGAAGAAAAATATGATCCAAAGA 1173
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 48727122590
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5