BLASTN 2.2.26+ Reference: Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000), "A greedy algorithm for aligning DNA sequences", J Comput Biol 2000; 7(1-2):203-14. Database: TAIR10_cdna_20110103_representative_gene_model_updated 33,602 sequences; 51,074,197 total letters Query= Ahg474631 Length=1167 Score E Sequences producing significant alignments: (Bits) Value AT1G55720.1 | Symbols: ATCAX6, CAX6 | cation exchanger 6 | chr1... 1696 0.0 AT1G55730.1 | Symbols: ATCAX5, CAX5 | cation exchanger 5 | chr1... 1487 0.0 AT3G13320.1 | Symbols: CAX2, atcax2 | cation exchanger 2 | chr3... 1044 0.0 > AT1G55720.1 | Symbols: ATCAX6, CAX6 | cation exchanger 6 | chr1:20828118-20830640 REVERSE LENGTH=1449 Length=1449 Score = 1696 bits (918), Expect = 0.0 Identities = 1093/1175 (93%), Gaps = 21/1175 (2%) Strand=Plus/Plus Query 1 ATGGAGCAAGGATCACTTCGAGAACGCAGAGCTAAAGCGCCTAGGAACAGTGTGATCCAG 60 |||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||| Sbjct 217 ATGGAGCAAGGATCACTTCGAGAACGCAGAGCTAAAGCGCCTAGGAACAGTGTGGTCCAG 276 Query 61 AGCTTCAAGATCGTGATTTTATCAAATAAACTCAATCTTTTGTTGCCTTTCGGTCCTCTA 120 |||||||||||||| |||||||| |||||||| ||||||||||||||||||||||||||| Sbjct 277 AGCTTCAAGATCGTCATTTTATCGAATAAACTAAATCTTTTGTTGCCTTTCGGTCCTCTA 336 Query 121 GCTATACTAGTCCATTACTTGACAGATACTAAGGGATGGTTCTTCTTACTAAGCTTAGTA 180 || ||||||||||| ||||||||||||| |||||| |||||||||||||||||||||||| Sbjct 337 GCAATACTAGTCCACTACTTGACAGATAATAAGGGGTGGTTCTTCTTACTAAGCTTAGTA 396 Query 181 GGCATCACACCATTAGCTGAACGTCTCGGATATGCCACAGAGCAATTGGCTTGTTACACA 240 || ||||||||||||||||||||||||||||||||||||||||||||| |||| |||||| Sbjct 397 GGTATCACACCATTAGCTGAACGTCTCGGATATGCCACAGAGCAATTGTCTTGCTACACA 456 Query 241 GGTTCAACTGTTGGAGGCCTCCTAAACGCTACATTTGGAAACGTGATAGAGCTGATCATA 300 || ||||||||||||| ||||| || |||||||||||||||||||| |||||||||||| Sbjct 457 GGCGCAACTGTTGGAGGTCTCCTCAATGCTACATTTGGAAACGTGATTGAGCTGATCATA 516 Query 301 TCGATTATCGCTCTTAAAAACGGGATGATACGCGTTGTACAGTTGACGCTGCTTGGCTCG 360 |||||||||||||||||||| || |||||||||||||||||||||||||||||||||||| Sbjct 517 TCGATTATCGCTCTTAAAAATGGAATGATACGCGTTGTACAGTTGACGCTGCTTGGCTCG 576 Query 361 ATTCTGTCTAACATGTTGCTTGTACTTGGGTGCGCCTTTTTCTGTGGCGGCCTTGTGTTT 420 ||||| |||||||| |||||||||||||| ||||| ||||||||||||||||||||| || Sbjct 577 ATTCTTTCTAACATTTTGCTTGTACTTGGCTGCGCTTTTTTCTGTGGCGGCCTTGTG-TT 635 Query 421 TCCC-GGAAAGATCAAGTCTTTGAC-AAAGGAAATGCAGTTGTGAGTTCGGGGATGCTTT 478 |||| |||||||||||||||||||| ||||| |||||||||||||||||||| ||||||| Sbjct 636 TCCCGGGAAAGATCAAGTCTTTGACAAAAGG-AATGCAGTTGTGAGTTCGGGCATGCTTT 694 Query 479 TATTGGCAGTCATGGGGCTGCTTTTCCCCACGGTTCTTCATTACACGCACAGCGAGGTTC 538 || ||||||||||||| || || |||||||| ||||||||||||||||||||||||||| Sbjct 695 TAATGGCAGTCATGGGACTACTCTTCCCCACATTTCTTCATTACACGCACAGCGAGGTTC 754 Query 539 ATGCCGGGTCATCAGAGCTTGCTCTCTCAAGATTTATCAGTTGTATAATGCTCCTTGCCT 598 |||| || ||||||||||||||||| || |||||||||||||||||||||||| |||||| Sbjct 755 ATGCTGGATCATCAGAGCTTGCTCTGTCGAGATTTATCAGTTGTATAATGCTCGTTGCCT 814 Query 599 ATGCAGCTTATCTCTTTTTCCAGTTAAAGAGCCAGCCAAGTTTTTATACCGAGAA--C-A 655 ||||||||||||||||||||||||||||||||||||||||||||||||||||||| | | Sbjct 815 ATGCAGCTTATCTCTTTTTCCAGTTAAAGAGCCAGCCAAGTTTTTATACCGAGAAAACTA 874 Query 656 A--AGAA--ATG----T-CAGAA-GATGATGAAGATCCCGAGATCTCCAAGTGGGAAGCT 705 | |||| | | | || || |||||||||||||| ||||||||||||||||||||| Sbjct 875 ACCAGAACGAAGAAACTTCA-AACGATGATGAAGATCCTGAGATCTCCAAGTGGGAAGCT 933 Query 706 ATCATCTGGCTTTCAATTTTTACGGCTTGGGTCTCTCTTCTTTCCGGCTATCTTGTTGAT 765 ||||||||||||||||| |||||||||||||||||||||||||| ||||||||||| ||| Sbjct 934 ATCATCTGGCTTTCAATCTTTACGGCTTGGGTCTCTCTTCTTTCGGGCTATCTTGTCGAT 993 Query 766 GCCATAGAGGGTGCTTCAGTCTCATGGAAGATACCAATCTCTTTTATCAGTGTCATCTTG 825 |||||||||||| |||| |||||||||||||||||||||||||||||||||||||||||| Sbjct 994 GCCATAGAGGGTACTTCGGTCTCATGGAAGATACCAATCTCTTTTATCAGTGTCATCTTG 1053 Query 826 CTTCCAATAGTTGGGAATGCGGCCGAGCATGCAGGTGCTATTATGTTTGCCATGAAAGAC 885 ||||| ||||||||||||||||| |||||||||||||| ||||||||||||||||||||| Sbjct 1054 CTTCCTATAGTTGGGAATGCGGCTGAGCATGCAGGTGCGATTATGTTTGCCATGAAAGAC 1113 Query 886 AAGCTGGATCTGTCTTTGGGAGTGGCTATTGGATCCTCAATTCAGATTTCTATGTTCGCG 945 |||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||| Sbjct 1114 AAGCTGGATCTGTCTTTGGGAGTGGCTATTGGATCCTCCATTCAGATTTCTATGTTCGCG 1173 Query 946 GTTCCTTTCTGTGTGGTGATCGGATGGATGATGGGTGTACAAATGGACCTAAATTTCCAG 1005 |||||||| |||||||||||||||||||||||||||| ||||||||| |||||| ||||| Sbjct 1174 GTTCCTTTTTGTGTGGTGATCGGATGGATGATGGGTGCACAAATGGATCTAAATCTCCAG 1233 Query 1006 CTATTTGAGACAGCTACTATGTTTATAACTGTTATTGTTGTAGCTTTCTTTCTCCAG--- 1062 ||||||||||| |||||| || | ||||||||||||||||||||||||||||||||| Sbjct 1234 CTATTTGAGACGGCTACTCTGCTCATAACTGTTATTGTTGTAGCTTTCTTTCTCCAGTTA 1293 Query 1063 GAAGGGACATCAAATTACTTCAAAGGACTGATGCTGATTCTTTGCTATTTGGTAGTCGCT 1122 ||||||||||| |||||||||||| || ||||||| ||||||||||||||| |||||||| Sbjct 1294 GAAGGGACATCGAATTACTTCAAAAGATTGATGCTCATTCTTTGCTATTTGATAGTCGCT 1353 Query 1123 GCCAGCTTCTTTGTACACGAAGATCCTCATCAAGG 1157 || || ||||||||||||||||||||||||||||| Sbjct 1354 GCTAGTTTCTTTGTACACGAAGATCCTCATCAAGG 1388 > AT1G55730.1 | Symbols: ATCAX5, CAX5 | cation exchanger 5 | chr1:20831052-20834519 REVERSE LENGTH=1933 Length=1933 Score = 1487 bits (805), Expect = 0.0 Identities = 1049/1164 (90%), Gaps = 27/1164 (2%) Strand=Plus/Plus Query 17 TTCGAGAACGCAGAGCTAAAGCGCCTAGGAACAGTGTGATCCAGAGCTTCAAGATCGTGA 76 |||||||| || | ||||| | |||| ||||||||| |||||||||||||||| |||| Sbjct 427 TTCGAGAATGCTCAACTAAAACACCTAATAACAGTGTGCTCCAGAGCTTCAAGATTGTGA 486 Query 77 TTTTATCAAATAAACTCAATCTTTTGTTGCCTTTCGGTCCTCTAGCTATACTAGTCCATT 136 ||||||| |||||||| ||||||||||||||||| |||||||| || |||||| |||||| Sbjct 487 TTTTATCGAATAAACTTAATCTTTTGTTGCCTTTTGGTCCTCTGGCAATACTACTCCATT 546 Query 137 ACTTGACAGATACTAAGGGATGGTTCTTCTTACTAAGCTTAGTAGGCATCACACCATTAG 196 |||||||||||| |||||||||| |||||||| | ||||||||||||||||||||||||| Sbjct 547 ACTTGACAGATAATAAGGGATGGATCTTCTTATTGAGCTTAGTAGGCATCACACCATTAG 606 Query 197 CTGAACGTCTCGGATATGCCACAGAGCAATTGGCTTGTTACACAGGTTCAACTGTTGGAG 256 |||||||||||||||||||||| |||||||| ||||||||||| |||||||||||||||| Sbjct 607 CTGAACGTCTCGGATATGCCACCGAGCAATTAGCTTGTTACACTGGTTCAACTGTTGGAG 666 Query 257 GCCTCCTAAACGCTACATTTGGAAACGTGATAGAGCTGATCATATCGATTATCGCTCTTA 316 | |||||||||||||||||||||||||||| ||||||||||||||||||| ||||||||| Sbjct 667 GTCTCCTAAACGCTACATTTGGAAACGTGACAGAGCTGATCATATCGATTTTCGCTCTTA 726 Query 317 AAAACGGGATGATACGCGTTGTACAGTTGACGCTGCTTGGCTCGATTCTGTCTAACATGT 376 ||| || |||||||| ||||||||| |||| |||| |||||||| || |||||||||| Sbjct 727 AAAGTGGAATGATACGTGTTGTACAGCTGACATTGCTCGGCTCGATACTTTCTAACATGT 786 Query 377 TGCTTGTACTTGGGTGCGCCTT-TTTCTGTGGCGGCCTTGTGTTTTCCCGGAAAGATCAA 435 | ||||||||||| |||||||| ||| |||||||| ||||| ||||||| |||||| ||| Sbjct 787 TACTTGTACTTGGCTGCGCCTTCTTT-TGTGGCGGGCTTGTATTTTCCCAGAAAGAGCAA 845 Query 436 GTCTTTGACAAAGGAAATGCAGTTGTGAGTTCGGGGAT-GCTTTT-ATTGGCAGTCATGG 493 |||||||||||||| ||||| ||||||| |||||| || |||||| || ||| ||||||| Sbjct 846 GTCTTTGACAAAGGTAATGCTGTTGTGAATTCGGG-ATTGCTTTTGAT-GGCTGTCATGG 903 Query 494 GGCTGCTTTTCCCCACGGTTCTTCATTACACGCACAGCGAGGTTCATGCCGGGTCATCAG 553 | || || |||||| | |||||||||||||||||||| ||||||||||| |||||||||| Sbjct 904 GACTACTCTTCCCCGCAGTTCTTCATTACACGCACAGTGAGGTTCATGCTGGGTCATCAG 963 Query 554 AGCTTGCTCTCTCAAGATTTATCAGTTGTATAATGCTCCTTGCCTATGCAGCTTATCTCT 613 ||||||||||||||||||| | |||||| ||||||||| |||||||||| ||||| |||| Sbjct 964 AGCTTGCTCTCTCAAGATTCAGCAGTTGCATAATGCTCGTTGCCTATGCTGCTTACCTCT 1023 Query 614 TTTTCCAGTTAAAGAGCCAGCCAAGTT-TT-TA----T---ACCGAG-AA-C-AA--AGA 659 |||||||||||||||||||||| || | || || | |||||| || | || ||| Sbjct 1024 TTTTCCAGTTAAAGAGCCAGCCGAGCTCTTATACCCCTCTCACCGAGGAAACTAACCAGA 1083 Query 660 A--ATG----T-CAGAAGATGATGAAGATCCCGAGATCTCCAAGTGGGAAGCTATCATCT 712 | | | | |||| |||||||||||||| ||||||||||||||||||||||| |||| Sbjct 1084 ACGAAGAAACTTCAGACGATGATGAAGATCCTGAGATCTCCAAGTGGGAAGCTATTATCT 1143 Query 713 GGCTTTCAATTTTTACGGCTTGGGTCTCTCTTCTTTCCGGCTATCTTGTTGATGCCATAG 772 |||||||||| || |||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1144 GGCTTTCAATCTTGACGGCTTGGGTCTCTCTTCTTTCCGGCTATCTTGTTGATGCCATAG 1203 Query 773 AGGGTGCTTCAGTCTCATGGAAGATACCAATCTCTTTTATCAGTGTCATCTTGCTTCCAA 832 |||||||||||||||||||||||||||||||||||||||||||||||||||| ||||| | Sbjct 1204 AGGGTGCTTCAGTCTCATGGAAGATACCAATCTCTTTTATCAGTGTCATCTTACTTCCTA 1263 Query 833 TAGTTGGGAATGCGGCCGAGCATGCAGGTGCTATTATGTTTGCCATGAAAGACAAGCTGG 892 ||||||||||||||||||||||||||||||| || ||||||||||||||||||||||||| Sbjct 1264 TAGTTGGGAATGCGGCCGAGCATGCAGGTGCCATCATGTTTGCCATGAAAGACAAGCTGG 1323 Query 893 ATCTGTCTTTGGGAGTGGCTATTGGATCCTCAATTCAGATTTCTATGTTCGCGGTTCCTT 952 ||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||| Sbjct 1324 ATCTGTCTTTGGGAGTGGCTATTGGATCCTCCATTCAGATTTCTATGTTCGCGGTTCCTT 1383 Query 953 TCTGTGTGGTGATCGGATGGATGATGGGTGTACAAATGGACCTAAATTTCCAGCTATTTG 1012 | || ||||| ||||||||||||||||||| |||||||||||||||||||||||||||| Sbjct 1384 TTTGCGTGGTTATCGGATGGATGATGGGTGCCCAAATGGACCTAAATTTCCAGCTATTTG 1443 Query 1013 AGACAGCTACTATGTTTATAACTGTTATTGTTGTAGCTTTCTTTCTCCAGGAAGGGACAT 1072 |||| |||||| |||| |||||||||||||||||||||||||||||||||||||| |||| Sbjct 1444 AGACGGCTACTCTGTTCATAACTGTTATTGTTGTAGCTTTCTTTCTCCAGGAAGGAACAT 1503 Query 1073 CAAATTACTTCAAAGGACTGATGCTGATTCTTTGCTATTTGGTAGTCGCTGCCAGCTTCT 1132 | ||||||||||||||| ||||||| ||||||||||||||| ||||||| || || |||| Sbjct 1504 CGAATTACTTCAAAGGATTGATGCTCATTCTTTGCTATTTGATAGTCGCCGCTAGTTTCT 1563 Query 1133 TTGTACACGAAGATCCTCATCAAG 1156 |||||||||||||||||||||||| Sbjct 1564 TTGTACACGAAGATCCTCATCAAG 1587 > AT3G13320.1 | Symbols: CAX2, atcax2 | cation exchanger 2 | chr3:4314529-4318351 FORWARD LENGTH=2120 Length=2120 Score = 1044 bits (565), Expect = 0.0 Identities = 966/1156 (84%), Gaps = 41/1156 (4%) Strand=Plus/Plus Query 32 CTAAAGCGCCTAGGAACAGTGTGA-TCCAGAGCTTCAAGATCGTGATTTTATCAAATAAA 90 ||||||| || | |||||| || | | | ||| | |||||||||||||| | |||||| Sbjct 610 CTAAAGCACCAAAGAACAGCGT-ACTTAATAGCATAAAGATCGTGATTTTTTGTAATAAA 668 Query 91 CTCAATCTTTTGTTGCCTTTCGGTCCTCTAGCTATACTAGTCCATTACTTGACAGATACT 150 |||||||| | | ||||||||||||||||| ||| | ||||| ||| ||| ||||| | Sbjct 669 CTCAATCTACTACTACCTTTCGGTCCTCTAGCCATATTGGTCCACTACATGATAGATAGT 728 Query 151 AAGGGATGGTTCTTCTTACT-AAGCTTAGTAGGCATCACACCATTAGCTGAACGTCTCGG 209 ||||| ||| | ||||| || | | ||||||||||| |||||||| ||||||||||| || Sbjct 729 AAGGGGTGGGTGTTCTTGCTGACG-TTAGTAGGCATTACACCATTGGCTGAACGTCTAGG 787 Query 210 ATATGCCACAGAGCAATTGGCTTGTTACACAGGTTCAACTGTTGGAGGCCTCCTAAACGC 269 |||||||||||||||| ||||||||||||| ||| ||||||||||||||||| |||| || Sbjct 788 ATATGCCACAGAGCAACTGGCTTGTTACACGGGTCCAACTGTTGGAGGCCTCTTAAATGC 847 Query 270 TACATTTGGAAACGTGATAGAGCTGATCATATCGATTATCGCTCTT-AAAAACGGGATGA 328 |||||| || || |||| ||||||||||||||| ||| ||||| || ||||| || |||| Sbjct 848 TACATTCGGGAATGTGACAGAGCTGATCATATCAATTTTCGCT-TTGAAAAATGGAATGA 906 Query 329 TACGCGTTGTACAGTTGACGCTGCTTGGCTCGATTCTGTCTAACATGTTGCTTGTACTTG 388 |||| ||||| ||| ||||| | || ||||| ||||||||||||||||| |||||||||| Sbjct 907 TACGTGTTGTTCAGCTGACGTTACTAGGCTCAATTCTGTCTAACATGTTACTTGTACTTG 966 Query 389 GGTGCGCCTT-TTTCTGTGGCGGCCTTGTGTTTTCCCGGAAAGATCAAGTCTTTGACAAA 447 | |||||||| ||| ||||| || || || |||| || ||||| ||||||||||||||| Sbjct 967 GCTGCGCCTTCTTT-TGTGGTGGGCTAGTCTTTTACCAAAAAGACCAAGTCTTTGACAAA 1025 Query 448 GGAAATGC-AGTTGTGAGTTCGGGGA-TGCTTTT-ATTGGCAGTCATGGGGCTGCTTTTC 504 |||| ||| | ||||| ||| ||| ||||||| | |||| ||||||||| | || ||| Sbjct 1026 GGAATTGCTA-CTGTGAATTC-AGGATTGCTTTTGA-TGGCTGTCATGGGGATACTCTTC 1082 Query 505 CCCACGGTTCTTCATTACACGCACAGCGAGGTTCATGCCGGGTCATCAGAGCTTGCTCTC 564 || | |||||||| ||||| || |||||||||||||| || ||||||||||| || || Sbjct 1083 CCGGCTGTTCTTCACTACACCCATAGCGAGGTTCATGCTGGATCATCAGAGCTAGCCCTG 1142 Query 565 TCAAGATTTATCAGTTGTATAATGCTCCTTGCCTATGCAGCTTATCTCTTTTTCCAGTTA 624 ||||| || | |||||| |||||||| | || ||||| ||||| ||||| ||||||||| Sbjct 1143 TCAAGGTTCAGCAGTTGCATAATGCTTATAGCTTATGCTGCTTACCTCTTCTTCCAGTTA 1202 Query 625 AAGAGCC-AGCCAAGTTTTTATA-----C-C---GA-GAA-C-AA--AGAA--ATG---- 663 ||||||| | | || || ||||| | | || ||| | || |||| | | Sbjct 1203 AAGAGCCAATCTAA-TTCTTATAGCCCTCTCGATGAGGAATCAAATCAGAACGAAGAAAC 1261 Query 664 -TCAGAAGATGATGAAGATCCCGAGATCTCCAAGTGGGAAGCTATCATCTGGCTTTCAAT 722 || | || ||||||||||| |||||||||||||||||||||||||| ||||||||||| Sbjct 1262 TTCTGCCGAAGATGAAGATCCTGAGATCTCCAAGTGGGAAGCTATCATTTGGCTTTCAAT 1321 Query 723 TTTTACGGCTTGGGTCTCTCTTCTTTCCGGCTATCTTGTTGATGCCATAGAGGGTGCTTC 782 || || |||||||||||||||||||| ||||||||||||||||||||||||||||| || Sbjct 1322 ATTGACTGCTTGGGTCTCTCTTCTTTCTGGCTATCTTGTTGATGCCATAGAGGGTGCCTC 1381 Query 783 AGTCTCATGGAAGATACCAATCTCTTTTATCAGTGTCATCTTGCTTCCAATAGTTGGGAA 842 |||||||||||| |||||||| ||||||||||| |||||||||||| ||||||||||| Sbjct 1382 AGTCTCATGGAATATACCAATAGCTTTTATCAGTACCATCTTGCTTCCTATAGTTGGGAA 1441 Query 843 TGCGGCCGAGCATGCAGGTGCTATTATGTTTGCCATGAAAGACAAGCTGGATCTGTCTTT 902 || || || |||||||| ||||| ||||||||||||||||||||||||||||||||||| Sbjct 1442 CGCAGCTGAACATGCAGGGGCTATCATGTTTGCCATGAAAGACAAGCTGGATCTGTCTTT 1501 Query 903 GGGAGTGGCTATTGGATCCTCAATTCAGATTTCTATGTTCGCGGTTCCTTTCTGTGTGGT 962 ||||||||||||||| ||||| || |||||||| ||||| ||||| || ||||| ||||| Sbjct 1502 GGGAGTGGCTATTGGGTCCTCGATCCAGATTTCCATGTTTGCGGTCCCATTCTGCGTGGT 1561 Query 963 GATCGGATGGATGATGGGTGTACAAATGGACCTAAATTTCCAGCTATTTGAGACAGCTAC 1022 ||| ||||||||||||||| ||| |||||||| || |||||||| |||||||| || | Sbjct 1562 GATTGGATGGATGATGGGTCAACAGATGGACCTTAACTTCCAGCTTTTTGAGACGGCGA- 1620 Query 1023 TA-TGTTTATAACTGTTATTGTTGTAGC-TTTCTTTCTCCAGGAAGGGACATCAAATTAC 1080 | |||| || |||||||| || ||||| ||| ||||||||||||||| |||| |||||| Sbjct 1621 TGCTGTTCATCACTGTTATAGTAGTAGCATTT-TTTCTCCAGGAAGGGTCATCGAATTAC 1679 Query 1081 TTCAAAGGACTGATGCTGATTCTTTGCTATTTGGTAGTCGCTGCCAGCTTCTTTGTACAC 1140 ||||||||| |||||||||||||||| |||||| |||| |||||||| |||||||||||| Sbjct 1680 TTCAAAGGATTGATGCTGATTCTTTGTTATTTGATAGTAGCTGCCAGTTTCTTTGTACAC 1739 Query 1141 GAAGATCCTCATCAAG 1156 ||||||||||| |||| Sbjct 1740 GAAGATCCTCACCAAG 1755 Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 57278821845 Database: TAIR10_cdna_20110103_representative_gene_model_updated Posted date: Sep 25, 2014 6:13 PM Number of letters in database: 51,074,197 Number of sequences in database: 33,602 Matrix: blastn matrix 1 -2 Gap Penalties: Existence: 0, Extension: 2.5