BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg474663
Length=2013
Score E
Sequences producing significant alignments: (Bits) Value
AT1G55930.1 | Symbols: | CBS domain-containing protein / trans... 1832 0.0
AT3G13070.1 | Symbols: | CBS domain-containing protein / trans... 863 0.0
> AT1G55930.1 | Symbols: | CBS domain-containing protein / transporter
associated domain-containing protein | chr1:20918717-20922232
FORWARD LENGTH=2239
Length=2239
Score = 1832 bits (992), Expect = 0.0
Identities = 1116/1174 (95%), Gaps = 16/1174 (1%)
Strand=Plus/Plus
Query 1 TAAAGAGGAGAAGAAATTGGTATACTCTATCGCCGGAG-A----C-----AT--TTCTAT 48
|||| ||| ||||||||||||| ||||||||||||||| | | || | ||||
Sbjct 115 TAAAAAGGGGAAGAAATTGGTAAACTCTATCGCCGGAGAATTTTCGGTTTATCCTCCTAT 174
Query 49 TCATATGGAACTTGATTTATCTGTTCTAGGTCGTTCTTTCATTGCCACCAGGAGGAACTC 108
|| ||||||||||||||||||||||||||||||||||||||||| |||||| ||||||||
Sbjct 175 TCGTATGGAACTTGATTTATCTGTTCTAGGTCGTTCTTTCATTGTCACCAGAAGGAACTC 234
Query 109 AAGTCTCACGCGACTATGTATCCAAAGTTCGAATTTTTCACTAAGACTTCTTCAGAGAAA 168
|||| ||||| ||| |||||||||||| |||||||||||| ||||| |||||||||||||
Sbjct 235 AAGTATCACGAGACCATGTATCCAAAGCTCGAATTTTTCAGTAAGAGTTCTTCAGAGAAA 294
Query 169 CAAGCAGCGCCCACTCTGTATCTTGACTAAT-C-TCTCAAGTTCCAGTTTTATTAGATTC 226
|||||| |||||||||||| ||| |||||| | || ||| |||||||||||||||||||
Sbjct 295 CAAGCATCGCCCACTCTGTTTCTCCACTAATCCGTC-CAA-TTCCAGTTTTATTAGATTC 352
Query 227 CGAAAGGGATGTGACTTTAGCCATAGAAATCAGTTTGTTGTGTTGAGTGCGACTGAAGAT 286
| ||| |||||||||||||| |||||| |||||||||||||||||||||||||| ||||
Sbjct 353 CAAAAAGGATGTGACTTTAGTCATAGATGTCAGTTTGTTGTGTTGAGTGCGACTGGAGAT 412
Query 287 CATGTGGGTAGTAGTCAGAAACATTCCGATTCCAGCGAAAAACTCGATTCGATTAGGATT 346
|||||||||| ||||||||||||||||||||||| ||||||| |||||||||||||||||
Sbjct 413 CATGTGGGTATTAGTCAGAAACATTCCGATTCCACCGAAAAAGTCGATTCGATTAGGATT 472
Query 347 TTGCTGAAACGGGGAATCGTTTTAGGGGCAGTGGTGTGTGGAGTTTTGTTGTATGGATGT 406
|||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
Sbjct 473 TTGCTGAAACGGGGAATCGTTTTAGGGGCAGTGGTGTGTGGAGTTTTGTTTTATGGATGT 532
Query 407 GGTAAAGTATTAGCATCAACAAATGTGGTGGATGTGGCTTTTAGTAAGAGTGTATTGTTG 466
|| ||||||||||||||||||| |||||||||||||||||||||||||||| ||||||||
Sbjct 533 GGGAAAGTATTAGCATCAACAAGTGTGGTGGATGTGGCTTTTAGTAAGAGTATATTGTTG 592
Query 467 TTGAAGAATGCTTGGCCAAAAACTTCTCAGGTTCTTAAGGTTCTTAGAGAGCAAGGTCTA 526
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 593 TTGAAGAATGCTTGGCCAAAAACTTCTCAGGTTCTTAAGGTTCTTAGAGAGCAAGGTCTA 652
Query 527 ATATTAGCTGTGCTTCTCGGTTTATCAGCATTCTTCTCCATGGCAGAGACTTCCATCACC 586
|| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 653 ATCTTAGCTGTGCTTCTCGGTTTATCAGCATTCTTCTCCATGGCAGAGACTTCCATCACC 712
Query 587 ACTCTCTGGCCGTGGAAGGTGCGCGAGTTGGCTGAAAAAGAGCCTGAAAATGGCGTTTTC 646
||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 713 ACTCTCTGGCCCTGGAAGGTGCGCGAGTTGGCTGAAAAAGAGCCTGAAAATGGCGTTTTC 772
Query 647 AGGATGCTCCGCAGTGATGTTACCAGATTCTTGACAACCATACTTATTGGAACAACTGTT 706
||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
Sbjct 773 AGGATGCTCCGCAGTGATGTTACCAGATTTTTGACAACCATACTTATTGGAACAACTGTT 832
Query 707 GTGAATATTGCGGCGACTGCCTTGGTCACTAAAGCAGCAACAGCTATTTTTGGTGAAGCT 766
|| |||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
Sbjct 833 GTTAATATTGCGGCGACTGCCTTGGTCACTAAAGCGGCAACAGCTATTTTTGGTGAAGCT 892
Query 767 GGTGTTAGTGCAGCCACAGGAGTGATGACGGTAGCCATATTACTCCTGACTGAAATAACT 826
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 893 GGTGTTAGTGCAGCCACAGGAGTGATGACGGTAGCCATATTACTCCTGACTGAAATAACT 952
Query 827 CCGAAAAGTGTAGCGGTTCACAATGCTCAAGAGGTTGCAAGGATTGTGGTCAGACCAGTG 886
|||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
Sbjct 953 CCGAAAAGTGTAGCGGTTCACAATGCTCAAGAGGTTGCCAGGATTGTGGTCAGACCAGTG 1012
Query 887 GCTTGGCTTTCCTTGATATTATATCCTGTTGGAAGAGTAGTGACATATCTTTCGATGGGA 946
|| |||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
Sbjct 1013 GCGTGGCTTTCCTTGATATTATATCCTGTTGGAAGAGTAGTAACATATCTTTCGATGGGA 1072
Query 947 ATTCTGAAAATTCTTGGTTTGAAAGGACGGAGTGAGCCATATGTTACTGAAGATGAATTG 1006
||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
Sbjct 1073 ATTCTGAAAATTCTTGGTTTGAAAGGACGGAGTGAACCATATGTTACTGAAGATGAATTG 1132
Query 1007 AAACTAATGCTACGCGGAGCAGAATTAAGTGGAGCTATAGAAGAGGAAGAGCAGGATATG 1066
|||||||||||||| || |||||||||||||| |||||||||||||||||||||||||||
Sbjct 1133 AAACTAATGCTACGTGGTGCAGAATTAAGTGGGGCTATAGAAGAGGAAGAGCAGGATATG 1192
Query 1067 ATTGAAAATGTGTTAGAGATAAAGGATACGCATGTTCGAGAGGTGATGACTCCTCTTGTT 1126
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1193 ATTGAAAATGTGTTAGAGATAAAGGATACGCATGTTCGAGAGGTGATGACTCCTCTTGTT 1252
Query 1127 GATGTAGTTGCAATAGATGGCAGCGGCAGCCTGG 1160
||||||||||||||||||||||||||||||||||
Sbjct 1253 GATGTAGTTGCAATAGATGGCAGCGGCAGCCTGG 1286
Score = 1373 bits (743), Expect = 0.0
Identities = 781/799 (98%), Gaps = 4/799 (1%)
Strand=Plus/Plus
Query 1215 AGGGTAAATTGCTTGAAAGTACCACTGTGGTGGACATGGCTCATAAACCTGCATTCTTTG 1274
|||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
Sbjct 1404 AGGGTAAATTGCTTGAAAGTACCACTGTGGTGGACATGGCACATAAACCTGCATTCTTTG 1463
Query 1275 TCCCTGATTCGATGTCAGTTTGGAATCTTCTTAGAGAGTTTAGAATAAGAAAGGTTCACA 1334
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1464 TCCCTGATTCGATGTCAGTTTGGAATCTTCTTAGAGAGTTTAGAATAAGAAAGGTTCACA 1523
Query 1335 TGGCAGTTGTTCTTAATGAATACGGTGGAACCATCGGAATAGTAACTCTTGAAGATGTGG 1394
|||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||
Sbjct 1524 TGGCAGTTGTTCTTAATGAATATGGTGGAACCATCGGAATAGTAACTCTTGAAGATGTGG 1583
Query 1395 TCGAAGAGATTGTTGGTGAAATATTTGATGAAAACGATTCAAAGGAGGAAATTCAGAAGA 1454
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1584 TCGAAGAGATTGTTGGTGAAATATTTGATGAAAACGATTCAAAGGAGGAAATTCAGAAGA 1643
Query 1455 AAACGGGTTATATCGTGATGAGAGCAGAGGGGATATATGATGTTGATGCCAACACTTCGA 1514
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1644 AAACGGGTTATATCGTGATGAGAGCAGAGGGGATATATGATGTTGATGCCAACACTTCGA 1703
Query 1515 TAGACCAGTTATCTGAAGAACTCAACATAAAGATGGCAGAGGGCCATCAGTATGAGACAG 1574
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1704 TTGACCAGTTATCTGAAGAACTCAACATAAAGATGGCAGAGGGCCATCAGTATGAGACAG 1763
Query 1575 TCTCAGGTTTTGTCTGTGAGGCCTTTGGGTATATCCCAAAGACAGGTGAGAGTGTGACGG 1634
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1764 TCTCAGGTTTTGTCTGTGAGGCCTTTGGGTATATCCCAAAGACAGGTGAGAGTGTGACGG 1823
Query 1635 TGGTTCTTGAAAAGGAAAACTGGGAAGAGAATGATGAACAAGACGAGGGTAAGCAGGAGC 1694
| ||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
Sbjct 1824 TAGTTCTTGAAAAGGAAAACTGGGAAGAGAATGATGAACAAGACGAGGGTAAGCACGAGC 1883
Query 1695 GTCAAGATCAGAAGGAGAAGCACCAAATTTATAGACTAGAGGTACTAGCAGGGAATGCCA 1754
||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
Sbjct 1884 GTCAAGATCAGAAGGAGAAGCACCAAATTTATAGACTAGAGATACTAGCAGGGAATGCCA 1943
Query 1755 GAAAAGTGAGTGCAGTCCGGTTCGAACGAGTTAATGATATGGATCAAGTGTCAGAGGCCC 1814
||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||
Sbjct 1944 GAAAAGTGAGTGCAGTCCGGTTCGAACGAGTTAGTGATATGGATCAAGTGTCAGAGGCCC 2003
Query 1815 GGGATGTGAAAAACATGGTTCCCAAGTTCGTAAGAAAATGGAGCAGCGAGGAAGATTCCG 1874
| ||||| ||||||||||||||||| |||||||||||||||||||| |||||||||||||
Sbjct 2004 GAGATGTAAAAAACATGGTTCCCAAATTCGTAAGAAAATGGAGCAGTGAGGAAGATTCCG 2063
Query 1875 ATGGTAATCTCCAAGCCAAGAATGCCGTCTTTGATGAGCATCTAATTGCTGAAACTGAAA 1934
||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
Sbjct 2064 ATGGTAATCTCCAAGCCAAGAATGCCGTCTTTGATGAGCATCTAATTGCAGAAACTGAAA 2123
Query 1935 GTATGAAGAAAGAATAATGATAAAAGAGGAGGAAAGAGGCAGTTGTAAGTTTTAATTGCA 1994
|||||||||||||||||||||||||||||| || |||| ||||||||||| |||||||
Sbjct 2124 GTATGAAGAAAGAATAATGATAAAAGAGGA--AA-GAGGT-GTTGTAAGTTTAAATTGCA 2179
Query 1995 TGTATAGAAAGACTCGTTA 2013
|||||||||||||||||||
Sbjct 2180 TGTATAGAAAGACTCGTTA 2198
> AT3G13070.1 | Symbols: | CBS domain-containing protein / transporter
associated domain-containing protein | chr3:4191351-4195112
REVERSE LENGTH=2146
Length=2146
Score = 863 bits (467), Expect = 0.0
Identities = 734/862 (85%), Gaps = 22/862 (3%)
Strand=Plus/Plus
Query 311 TCCGATTCCAGCGAAAA--A-CTCGATTCGATT-AGGATTTTGCTGAAACGGGGAATCGT 366
|||||||| | ||||| | || || |||||| ||| ||||||||||||| ||||| ||
Sbjct 262 TCCGATTCTGGTGAAAAGGAGCTGGAGTCGATTAAGG-TTTTGCTGAAACGTGGAATTGT 320
Query 367 TTTAGGGGCAGTGGTGTGTGGAGTTTTGTTGTATGGATGT-GGTAAAGTATTAGCATCAA 425
|||| || | || ||||||||||| |||||||||||| | ||||| || |||||
Sbjct 321 GATAGGAGCTCTAGTCTGTGGAGTTTTCTTGTATGGATGTCAG-AAAGTGTTGGCATCGG 379
Query 426 CAAATGTGGT-G--G-A---TGTG-GCT-TTTAGTAAGAGTGTATTGTTGTTGAAGAATG 476
| |||||| | | | | || | | ||| || |||| ||| ||||||| |||||||
Sbjct 380 CTGGTGTGGTCGAAGCAGGCTATGAGGTGTTTGGTCAGAGCGTAGTGTTGTTTAAGAATG 439
Query 477 CTTGGCCAAAAACTT-CTCAGGTTCTTAAGGTTCTTAGAGAGCAAGGTCTAATATTAGCT 535
||| ||| |||| || | |||||||||| |||||||||||||||||||||||| || |||
Sbjct 440 CTTTGCCTAAAATTTAC-CAGGTTCTTACGGTTCTTAGAGAGCAAGGTCTAATTTTGGCT 498
Query 536 GTGCTTCTCGGTTTATCAGCATTCTTCTCCATGGCAGAGACTTCCATCACCACTCTCTGG 595
| ||| ||| | | |||||||||||||| ||||||||||| ||||| |||||||| |||
Sbjct 499 GCGCTGCTCAGCCTCTCAGCATTCTTCTCTATGGCAGAGACGTCCATTACCACTCTGTGG 558
Query 596 CCGTGGAAGGTGCGCGAGTTGGCTGAAAAAGAGCCTGAAAATGGCGTTTTCAGGATGCTC 655
|||||||||||||| ||||||||||| ||||| || || ||||| || ||||| ||||||
Sbjct 559 CCGTGGAAGGTGCGTGAGTTGGCTGAGAAAGAACCAGAGAATGGTGTATTCAGAATGCTC 618
Query 656 CGCAGTGATGTTACCAGATTCTTGACAACCATACTTATTGGAACAACTGTTGTGAATATT 715
|| |||||||| |||||||||||||| || ||||||||||||||||||||||||||||||
Sbjct 619 CGGAGTGATGTCACCAGATTCTTGACGACTATACTTATTGGAACAACTGTTGTGAATATT 678
Query 716 GCGGCGACTGC-CTTGGTCACTAAAGCAGCAACAGCTATTTTTGGTGAAGCTGGTGTTAG 774
||||| ||||| || ||||||| |||||||||||||||| ||||| ||||||||||||||
Sbjct 679 GCGGCAACTGCACT-GGTCACTGAAGCAGCAACAGCTATATTTGGGGAAGCTGGTGTTAG 737
Query 775 TGCAGCCACAGGAGTGATGACGGTAGCCATATTACTCCTGACTGAAATAACTCCGAAAAG 834
||| ||||| ||| ||||||| || || || |||||||||||||| || ||||| |||||
Sbjct 738 TGCTGCCACTGGATTGATGACAGTGGCTATTTTACTCCTGACTGAGATTACTCCAAAAAG 797
Query 835 TGTAGCGGTTCACAATGCTCAAGAGGTTGCAAGGATTGTGGTCAGACCAGTGGCTTGGCT 894
||| || || |||||||||||||| |||||||||||||||||||| |||||||| |||||
Sbjct 798 TGTTGCTGTGCACAATGCTCAAGAAGTTGCAAGGATTGTGGTCAGGCCAGTGGCGTGGCT 857
Query 895 TTCCTT-GATATTATATCCTGTTGGAAGAGTAGTGACATATCTTTCGATGGGAATTCTGA 953
|||||| | ||||||||||||| |||||| | || |||||||| || |||||||| ||||
Sbjct 858 TTCCTTAG-TATTATATCCTGTCGGAAGAATTGTCACATATCTGTCAATGGGAATACTGA 916
Query 954 AAATTCTTGGTTTGAAAGGACGGAGTGAGCCATATGTTACTGAAGATGAATTGAAACTAA 1013
|||||||||||||||||||||||||||| |||||||||||||||||||| |||||||| |
Sbjct 917 AAATTCTTGGTTTGAAAGGACGGAGTGAACCATATGTTACTGAAGATGAGTTGAAACTGA 976
Query 1014 TGCTACGCGGAGCAGAATTAAGTGGAGCTATAGAAGAGGAAGAGCAGGATATGATTGAAA 1073
||||| | || |||||||| ||||| || || ||||| || |||||||||||||||||||
Sbjct 977 TGCTAAGAGGTGCAGAATTGAGTGGTGCCATTGAAGAAGAGGAGCAGGATATGATTGAAA 1036
Query 1074 ATGTGTTAGAGATAAAGGATACGCATGTTCGAGAGGTGATGACTCCTCTTGTTGATGTAG 1133
||||| ||||||| |||||||| ||||||||||||||||||||||| |||||||||||||
Sbjct 1037 ATGTGCTAGAGATTAAGGATACCCATGTTCGAGAGGTGATGACTCCCCTTGTTGATGTAG 1096
Query 1134 TTGCAATAGATGGCAGCGGCAG 1155
| ||||| || | ||||| |||
Sbjct 1097 TCGCAATTGACGCCAGCGCCAG 1118
Score = 710 bits (384), Expect = 0.0
Identities = 564/650 (87%), Gaps = 16/650 (2%)
Strand=Plus/Plus
Query 1229 GAAAGTACCACTGTGGTGGACATGGCTCATAAACCTGCATTCTTT-GTCCCTGATTCGAT 1287
|||||||| |||||| ||||||||| |||||||||||| | ||| ||||||||||| ||
Sbjct 1255 GAAAGTACTTCTGTGGGGGACATGGCACATAAACCTGCA-TATTTCGTCCCTGATTCAAT 1313
Query 1288 GTCAGTTTGGAATCTTCTTAGAGAGTTTAGAATAAGAAAGGTTCACATGGCAGTTGTTCT 1347
||| |||||||||||||||||||| || |||||||||||||||||||||||||||| ||
Sbjct 1314 GTCTGTTTGGAATCTTCTTAGAGAATTCCGAATAAGAAAGGTTCACATGGCAGTTGTCCT 1373
Query 1348 TAATGAATACGGTGGAACCATCGGAATAGTAACTCTTGAAGATGTGGTCGAAGAGATTGT 1407
||| ||||| |||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 1374 TAACGAATATGGTGGAACGATCGGAATAGTAACTCTTGAAGATGTGGTCGAAGAGATTGT 1433
Query 1408 TGGTGAAATATTTGATGAAAACGATTC-AAAGGAGGAAATTCAGAAGAAAACGGGTTATA 1466
||||||||| ||||||||||||||||| |||| |||| |||||||||||||| || ||||
Sbjct 1434 TGGTGAAATTTTTGATGAAAACGATTCCAAAG-AGGAGATTCAGAAGAAAACAGGATATA 1492
Query 1467 TCGTGATGAGAGCA-GAGGGGATATATGATGTTGATGCCAACACTTCGATAGACCAGTTA 1525
| || ||||||| | ||||| ||||||||||||||||| || || || || |||||| ||
Sbjct 1493 TTGTCATGAGAG-ATGAGGGCATATATGATGTTGATGCTAATACATCAATTGACCAGCTA 1551
Query 1526 TCTGAAGAACTCAACAT-AAAGATGGCAGAGGGCCAT-CAGTATGAGACAGTCTCAGGTT 1583
||||||||||| || || ||| ||| |||||||| || ||||| |||||||||||||| |
Sbjct 1552 TCTGAAGAACTGAATATGAAA-ATGCCAGAGGGC-ATTCAGTACGAGACAGTCTCAGGCT 1609
Query 1584 TTGTCTGTGAGGCCTTTGGGTATATCCCAAAGACAGGTGAGAGTGTGACGGTGGTTCTTG 1643
|||| |||||||||||||||||||||||||| || || || ||||||| ||| |||||||
Sbjct 1610 TTGTGTGTGAGGCCTTTGGGTATATCCCAAAAACTGGAGAAAGTGTGAAGGTTGTTCTTG 1669
Query 1644 AAAAGGAAAACTGGGAAGAGAATGATGAACAAGACGAGGGTAAGCAGGAGCGTCAAGATC 1703
||||||||| |||||||||| ||| ||| | || || ||||| |||||| | ||||| |
Sbjct 1670 AAAAGGAAAGCTGGGAAGAGGATGGTGAGGAGGAGGAAGGTAAACAGGAGAGGCAAGAGC 1729
Query 1704 -AGAAGGAGAAGCACCAAATTTATAGACTAGAGGTACTAGCAGGGAATGCCAGAAAAGTG 1762
| ||||| ||| ||||||| |||||| ||||| |||||||||||||||| ||||||||
Sbjct 1730 CA-AAGGAAAAGAACCAAATCTATAGAGTAGAGATACTAGCAGGGAATGCAAGAAAAGTT 1788
Query 1763 AGTGCAGTCCGGTTCGA-ACGAGTTAATGATATGGATCAAGTGTCAGAGGCCCGGGATGT 1821
||||| |||||||| || | |||| || ||||||||||||||||| ||||| | |||||
Sbjct 1789 AGTGCTGTCCGGTTTGAGA-GAGTGAACGATATGGATCAAGTGTCGGAGGCAAGTGATGT 1847
Query 1822 GAAAAA-CATGGTTCCCAAGTTCGTAAGAAAATGGAGCAGCGAGGAAGAT 1870
||||| |||||||||||| || ||||| |||||||||||||| ||||||
Sbjct 1848 -AAAAAGCATGGTTCCCAAATTTGTAAGGAAATGGAGCAGCGAAGAAGAT 1896
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 99748482915
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5