BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg476275
Length=1739
Score E
Sequences producing significant alignments: (Bits) Value
AT1G71440.1 | Symbols: PFI, TFC E | tubulin folding cofactor E ... 2466 0.0
> AT1G71440.1 | Symbols: PFI, TFC E | tubulin folding cofactor
E / Pfifferling (PFI) | chr1:26921060-26924386 REVERSE LENGTH=1820
Length=1820
Score = 2466 bits (1335), Expect = 0.0
Identities = 1518/1607 (94%), Gaps = 10/1607 (1%)
Strand=Plus/Plus
Query 1 AAAATGAAGGCTGAGTCAAGCAACGAGTCTTAT-AAAATCGGTCAGCGAGTCCACTCGCT 59
||||||||||||||||| ||||||||||||| | | |||||| |||||||||||||| ||
Sbjct 11 AAAATGAAGGCTGAGTCTAGCAACGAGTCTT-TCATAATCGGGCAGCGAGTCCACTCTCT 69
Query 60 TAACGATCCGCGTCGGGTGGGTACCGTGAAGTACGTCGGAGATGTGGAGGGATACTCCGG 119
|||||| ||||||| ||||| |||||||| |||||||||||||||||||||||||||||
Sbjct 70 CAACGATTCGCGTCGTGTGGGCACCGTGAAATACGTCGGAGATGTGGAGGGATACTCCGG 129
Query 120 AACTTGGATCGGCGTTGATTGGGATCAAGACGGCGATGGAAAGCATAACGGTACCGTCAA 179
||||||||||||||||||||||||||||||||| |||||||| ||||||||| |||||||
Sbjct 130 AACTTGGATCGGCGTTGATTGGGATCAAGACGGTGATGGAAAACATAACGGTTCCGTCAA 189
Query 180 TGGCGTTTTCTACTTTAACGGTCGTTCTCAAAGCTCAGCTTCATTTGTTCGTTCCCAGAA 239
|||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
Sbjct 190 TGGCGTTTTCTACTTTAACGGTCGTTCTCAGAGCTCAGCTTCATTTGTTCGTTCCCAGAA 249
Query 240 TCTTAGTCGTGGTATCACATTGCTTCAAGCTTTGGAGCTTAGATACAGAACAACTTCAAC 299
||||||||| ||||||||||||||||||||||||||||||||||||||||||| ||||||
Sbjct 250 TCTTAGTCGCGGTATCACATTGCTTCAAGCTTTGGAGCTTAGATACAGAACAATTTCAAC 309
Query 300 TAAGGACGAAGAAGATGAAATGTATGTTCTTTCTGCTGGGAATAGACGTGTTTCGATTCA 359
|||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
Sbjct 310 TAAGGACGAAGAAGATGAAATGTATGTTCTCTCTGCTGGGAATAGACGTGTTTCGATTCA 369
Query 360 GCTATTAGGAGGGGATAAGATCCAGGATAAGTTAAGTCGGTTCGAGGAGCTTACGAGTGC 419
||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 370 GCTTTTAGGAGGGGATAAGATCCAGGATAAGTTAAGTCGGTTCGAGGAGCTTACGAGTGC 429
Query 420 TTCTCTTTCGTATCTTGGTGTTAGTTCTCTTGGAGTTTCATCTGACTTGGGTTCAATTCT 479
||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||
Sbjct 430 TTCTCTTTCGTATCTTGGTGTTAGTTCTCTTGGAGTTTCATCTGATTTGGGTTCAATTCT 489
Query 480 ACCAAATTTGAAGCTACTGGACTTAACTGGAAACTTGATTTCTGACTGGGAGGAAATTGG 539
||||||||||||||| |||||||||||||||||||||||||||||||||||||| |||||
Sbjct 490 ACCAAATTTGAAGCTGCTGGACTTAACTGGAAACTTGATTTCTGACTGGGAGGAGATTGG 549
Query 540 TGCTCTTTGTGAGCAGTTACCAGCCCTTACAACCCTTAATTTGTCTTGCAATTCTTTGTC 599
|||||||||||| ||||||||||| |||||||||||||||||||||||||||||||||||
Sbjct 550 TGCTCTTTGTGAACAGTTACCAGCACTTACAACCCTTAATTTGTCTTGCAATTCTTTGTC 609
Query 600 AAGTGATATCACCTCTCTGCCACAGTTAAAAAATATACGTGTTTTGGTTCTGAATAATAG 659
||||||||||| ||||||||||||||||||||||| |||||||||||||||||||||||
Sbjct 610 AAGTGATATCAAGTCTCTGCCACAGTTAAAAAATATCCGTGTTTTGGTTCTGAATAATAG 669
Query 660 TGGTCTGAGCTGGACTCAGGTTGAAATTCTTAGGCGCTCGTTACCTGGACTTGAGGAGTT 719
||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
Sbjct 670 TGGTCTGAGCTGGACTCAGGTTGAAATTCTTAGGCGCTCGTTACCTGGAATTGAGGAGTT 729
Query 720 GCATCTGATGGGGAATATGATATCTGCCTTAACTTCTACATC-TCCCTCAGATGGTCAAG 778
||||||||||||||| ||||||||| || |||| || || || || ||||||| |||||
Sbjct 730 GCATCTGATGGGGAACATGATATCTACCATAACATCCACGTCCTCA-TCAGATGATCAAG 788
Query 779 CATTTAACTCTCTGCGACTTCTAAACTTGGATGATAATTGTATTTCTGATTGGAGTGAAG 838
|||||||||||||||| || || ||||||||||||||||||||||| |||||||||||||
Sbjct 789 CATTTAACTCTCTGCGGCTACTGAACTTGGATGATAATTGTATTTCCGATTGGAGTGAAG 848
Query 839 TATTAAAGCTATCTCAACTTCCATGCTTGGAGCAGCTTTACCTGAACAAGAATAAGCTGA 898
|||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 849 TATTAAAGCTATCTCAACTTCCATGCTTGGAGCAGCTTTACCTGAATAAGAATAAGCTTT 908
Query 899 CACACATCTTTCACTCTGTAAATGGCATTGAATCGCCAAAGAAAAGCAGTGACCCGTTTC 958
||| ||||||||| ||||||||||||| |||||| || ||||| |||| ||||| ||||
Sbjct 909 CACGCATCTTTCAGTCTGTAAATGGCACTGAATCATCAGAGAAAGGCAGCGACCCATTTC 968
Query 959 CAAGTCTGCGTTGTCTTCTTCTAGGAGCCAACAGCATTGGCGATTTGGCCTC-GATAGAC 1017
| |||||| |||||||||||||||||||||||| |||||||||||||||||| | |||||
Sbjct 969 CTAGTCTGAGTTGTCTTCTTCTAGGAGCCAACAACATTGGCGATTTGGCCTCAG-TAGAC 1027
Query 1018 GCACTGAATGTGTTTCCTCAGTTAGTGGACATCAGGCTTTCTGAAAACCCAATATCTGAT 1077
|||||||||| |||||||||||||||||||||||||||||| ||||||||||||||||||
Sbjct 1028 GCACTGAATGGGTTTCCTCAGTTAGTGGACATCAGGCTTTCGGAAAACCCAATATCTGAT 1087
Query 1078 CCTGTAAGAGGTGGTGTCCCAAGATTTGTTCTCGTTGCAAGGTTGACAAAAGTACAAGTT 1137
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1088 CCTGTAAGAGGTGGTGTCCCAAGATTTGTTCTCGTTGCAAGGTTGACAAAAGTACAAGTG 1147
Query 1138 CTGAATGGGAGTGAGGTAAGAGCTCGTGAGAAGAAGGATTCTGAGATCCGGTATGTCCGA 1197
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1148 CTGAATGGGAGTGAGGTAAGAGCTCGTGAGAAGAAGGATTCTGAGATCCGGTATGTCCGA 1207
Query 1198 ATGGTGATGTCAAAGTTAAATGACAAGTCTGAGGAGATTGAGCTGCTCCACCCTAGGTTT 1257
||||||||||||||||||||||||||||||| |||||||||||||||||||||||| |||
Sbjct 1208 ATGGTGATGTCAAAGTTAAATGACAAGTCTGGGGAGATTGAGCTGCTCCACCCTAGATTT 1267
Query 1258 TATGAACTCAAGAAACTTCACGGAATTGAGGATGAAAGAGCATCAGCTGAGAACAGCGGC 1317
|||||||||||||||||||| ||||||||||| |||||||||||||||||||||||||||
Sbjct 1268 TATGAACTCAAGAAACTTCATGGAATTGAGGACGAAAGAGCATCAGCTGAGAACAGCGGC 1327
Query 1318 CCTAAAAATATGGCATCTGGACTTTTATCCATTGCTCTCAAATGCGTTGGTCCATCAATG 1377
||||| ||||| |||||||||||| | |||||| ||||||||||||||||||||||||||
Sbjct 1328 CCTAAGAATATAGCATCTGGACTTATCTCCATTACTCTCAAATGCGTTGGTCCATCAATG 1387
Query 1378 GGTGAAAAGCCTGAGTTGACaaaaaaaTTGCCGGGCAGTATCACGGTCGGGAAGCTGAAG 1437
|||||||| ||| | |||||||| || || ||||||||||||||||| || |||||||||
Sbjct 1388 GGTGAAAAACCTCACTTGACAAAGAAGTTACCGGGCAGTATCACGGTTGGAAAGCTGAAG 1447
Query 1438 ATCCTATGTGAGAACTTCTTT-AAGCTCAAGTCCATTAAGCCAAGATTACTTT-TGCAGG 1495
||| ||| ||||||||| ||| |||||||||||||| |||||||||||| ||| ||||||
Sbjct 1448 ATCTTATCTGAGAACTT-TTTCAAGCTCAAGTCCATCAAGCCAAGATTA-TTTCTGCAGG 1505
Query 1496 AGGAGGGCTCACCTTTTCCGACAGCACTCGATGACGAGACTGCAACATTACTGGACGTTG 1555
| |||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
Sbjct 1506 AAGAGGGCTCACCTTTTCCGACAGCACTCGATGACGAGACTGCAACATTACTTGACGTTG 1565
Query 1556 GAATCTGCGACGGTTCCACACTTCTTGTTGACGAAGAGAGTTAAGTT 1602
||||||||||||| |||||||||||||||||||||||||||||||||
Sbjct 1566 GAATCTGCGACGGCTCCACACTTCTTGTTGACGAAGAGAGTTAAGTT 1612
Score = 126 bits (68), Expect = 6e-28
Identities = 130/156 (83%), Gaps = 19/156 (12%)
Strand=Plus/Plus
Query 1601 TTTATTTTATGGATTGTCACTGTGAACATTAATAGAT-ATCCCATCTTTTGCAAGTGACT 1659
||||||||||||||||||||||||||||||||||| | |||| ||||||||||||| |||
Sbjct 1655 TTTATTTTATGGATTGTCACTGTGAACATTAATAGTTTATCC-ATCTTTTGCAAGTAACT 1713
Query 1660 TGAAC--AC--AAGGACC--AA-A-----A---CCATCATCAACATTCCAAAAGTTTCAT 1704
||||| || || || || | | ||||||||| |||| ||||||||||||
Sbjct 1714 TGAACCCACGTAAAAACATTAATAGTTCCAGAACCATCATCATCATTGCAAAAGTTTCAT 1773
Query 1705 ACAAAGT-aaaaaaaa-TTGAAATTGAGAAGAGACA 1738
||||||| |||||||| |||||||||||||||||||
Sbjct 1774 ACAAAGTTAAAAAAAAATTGAAATTGAGAAGAGACA 1809
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 85993533585
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5