BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg480542
Length=1149
Score E
Sequences producing significant alignments: (Bits) Value
AT2G16530.1 | Symbols: | 3-oxo-5-alpha-steroid 4-dehydrogenase... 1701 0.0
AT1G72590.1 | Symbols: | 3-oxo-5-alpha-steroid 4-dehydrogenase... 872 0.0
AT3G43840.1 | Symbols: | 3-oxo-5-alpha-steroid 4-dehydrogenase... 209 4e-53
> AT2G16530.1 | Symbols: | 3-oxo-5-alpha-steroid 4-dehydrogenase
family protein | chr2:7162989-7165323 REVERSE LENGTH=1427
Length=1427
Score = 1701 bits (921), Expect = 0.0
Identities = 1076/1150 (94%), Gaps = 14/1150 (1%)
Strand=Plus/Plus
Query 2 TGGAGTTAGAGATTGTTTGGTTGGTTAGAGCCGCTTGGATCACCGTTTGGGTCGTCTCTA 61
|||| || |||||||| ||||||||||||| |||||||||||| |||||| |||||||||
Sbjct 113 TGGAATTGGAGATTGTGTGGTTGGTTAGAGGCGCTTGGATCACTGTTTGGATCGTCTCTA 172
Query 62 TACTTCCTTTGGTGATTGCTTCGATACCCACTTCAAAGCTTAACTCTTTTCGTGAACTCG 121
|||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
Sbjct 173 TACTTCCTTTGGTGATTGCTTCGATACCCACTTCAAAGCTCAACTCTTTTCGTGAACTCG 232
Query 122 TTTTGAGTTTCGCTGGTAGAGGCAAGATTCTTCACCCTTCTTCTCAGAAGTTTACAGTTC 181
|||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
Sbjct 233 TTTTGAGTTTCGCTGGTAGAGGCAAGATTCTTCACCCTTCTTCTCAGAAGTTTACAATTC 292
Query 182 CACAGAAGTTCTTTGCTCACTTCTATGTGATTGGAGTGGTGTGGACGACTCTTCTGCTTG 241
| ||||| | |||||| ||||| ||||| ||||||||||||||||| ||||| |||||||
Sbjct 293 CTCAGAAATGCTTTGCACACTTTTATGTTATTGGAGTGGTGTGGACAACTCTCCTGCTTG 352
Query 242 CTGCAACTTGGATGTATGCTTGCGAAATGGCGCCTTTATCTTCTGAGGAGTTCCAGTTAT 301
||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
Sbjct 353 CTGCAACTTGGATGTATGCTTGCAAAATGGCGCCTTTATCTTCTGAGGAGTTCCAGTTAT 412
Query 302 CCGACATTGCAAGCCGTTTAGCCGGAGGTTCAGATGTTTTCTCTTTTCATAAATCTCATA 361
||||||||||||||||||||||||||||||||||||||||||| ||||||||||| |||
Sbjct 413 CCGACATTGCAAGCCGTTTAGCCGGAGGTTCAGATGTTTTCTCCGTTCATAAATCTAATA 472
Query 362 TGACTCCTGTTGAGGATCGGTTTGAAGTGTGGCGAGCGGTATTTCTACTTCTTGTGATGG 421
|||||||||| ||| |||||||| |||||||||||||||| |||||||||||| ||||||
Sbjct 473 TGACTCCTGTCGAGCATCGGTTTAAAGTGTGGCGAGCGGTGTTTCTACTTCTTCTGATGG 532
Query 422 AGATTCATGTCTTGAGACGGGTTATAGAGTCATTCTATGTATTCAAATATAGCCCTTCCG 481
|||| ||||||||||||||| ||||||||||||||||||| ||||||||||||||||| |
Sbjct 533 AGATCCATGTCTTGAGACGGCTTATAGAGTCATTCTATGTTTTCAAATATAGCCCTTCTG 592
Query 482 CTCGGATGCACATTCTCGGTTATTTCGCAGGATTGTTTTTCTATGTAACAGCACCTTTGT 541
|||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
Sbjct 593 CTCGGATGCACATTCTCGGTTATTTCGCAGGATTGTTTTTCTATGTAACAGCTCCTTTGT 652
Query 542 CACTCTGCTCCAATATTGCTCCAGAGGTAGCAAGATTTGTAGGAAATCAAGTGGCTGAGT 601
|||||||||||||||| ||||||||||||||| |||| ||||||||||||||||||||||
Sbjct 653 CACTCTGCTCCAATATAGCTCCAGAGGTAGCAGGATTCGTAGGAAATCAAGTGGCTGAGT 712
Query 602 TCATTGCTAATGGGAAAAGTCATACTTCAGCACCTGAA-TTAAGTTTAGTAAT-GTGTAT 659
||||||||||||||||||| |||||||||||||||||| |||| || || || | |||
Sbjct 713 TCATTGCTAATGGGAAAAGCCATACTTCAGCACCTGAATTTAA-CTT-GTTATCATCTAT 770
Query 660 AAGTCCTTTGATGAAGCTTGGATCAGTCCAGTGGATTGGTGGAGCCATTTTTCTCTGGGG 719
||||||||||||||||||||||||| |||||||||||||||||||||||||||| |||||
Sbjct 771 AAGTCCTTTGATGAAGCTTGGATCACTCCAGTGGATTGGTGGAGCCATTTTTCTTTGGGG 830
Query 720 ATGGATACATCAGCGCCGCTGTCACGCCATTCTTGGATCGCTCCGGGAAAATCCGAGCCA 779
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 831 ATGGATACATCAGCGCCGCTGTCACGCCATTCTTGGATCGCTCCGGGAAAATCCGAGCCA 890
Query 780 AGCGAAAGAGTACATAATTCCGTATGGGGATTGGTTCAGGATGGTCTCTTCTCCTCATTT 839
||||||||||||||| ||||||||||| |||||||| ||||||| |||||||| |||||
Sbjct 891 AGCGAAAGAGTACATTATTCCGTATGGAGATTGGTTTGGGATGGTGTCTTCTCCACATTT 950
Query 840 CTTGGCAGAAATCGTTTTATATGCGGGTTTACTGATTGCGAGTGGAGGAACAGATATAAC 899
|||||||||||| ||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 951 CTTGGCAGAAATTGTTTTGTATGCGGGTTTACTGATTGCGAGTGGAGGAACAGATATAAC 1010
Query 900 CATCTGGTTACTCTTTGGTTTCGTGGCTGCTAATCTGACATACGCGGCTGGAGAAACACA 959
||||||||||||||||||||| ||||||||||||||||||||||||||||||||||| ||
Sbjct 1011 CATCTGGTTACTCTTTGGTTTTGTGGCTGCTAATCTGACATACGCGGCTGGAGAAACGCA 1070
Query 960 CCGTTGGTATCTCCGAAAGTTTGAGGATTATCCGGCTAATCGGCATGCCATTTTCCCTCA 1019
||||||||||||||| ||||||||| |||||||||||||||||||||||||||||||| |
Sbjct 1071 CCGTTGGTATCTCCGGAAGTTTGAGAATTATCCGGCTAATCGGCATGCCATTTTCCCTTA 1130
Query 1020 TGTCTACTAACTGGTTAAGAGATACAATGGTTGAATATTTCATGTTTCTGTAAATGCTGA 1079
|||||||||| |||||||||||||||||||||| | | | | |||||||||||||
Sbjct 1131 TGTCTACTAAATGGTTAAGAGATACAATGGTTG--T----C-T-T--CTGTAAATGCTGA 1180
Query 1080 ATTTTGAAGATCAAAGATTTGTGGTGCTTTGTTGTGTTATAATTTAGCTATGCTTCTCAA 1139
||| |||| ||| |||||||||||||||||||| |||| ||||||||||||||||||||
Sbjct 1181 ATTCTGAACATCGAAGATTTGTGGTGCTTTGTTATGTTTTAATTTAGCTATGCTTCTCAG 1240
Query 1140 TTCTCATCTT 1149
||||||||||
Sbjct 1241 TTCTCATCTT 1250
> AT1G72590.1 | Symbols: | 3-oxo-5-alpha-steroid 4-dehydrogenase
family protein | chr1:27335601-27337072 FORWARD LENGTH=1046
Length=1046
Score = 872 bits (472), Expect = 0.0
Identities = 619/688 (90%), Gaps = 18/688 (3%)
Strand=Plus/Plus
Query 358 CATATGACTCCTGTTGAGGATCGGTTTGAAGTGTGGCGAGCGGTATTTCTACTTCTTGTG 417
|||||||||| ||||||| |||||||| ||||| |||||||||| |||||||||||| ||
Sbjct 298 CATATGACTCATGTTGAGCATCGGTTTAAAGTGGGGCGAGCGGTGTTTCTACTTCTTCTG 357
Query 418 ATGGAGATTCATGTCTTGAGACGGGTTATAGAGTCATTCTATGTATTCAAATATAGCCCT 477
|||||||| |||||||||||||| ||||||||||||||||||||||||||||||||| ||
Sbjct 358 ATGGAGATCCATGTCTTGAGACGTGTTATAGAGTCATTCTATGTATTCAAATATAGCACT 417
Query 478 TCCGCTCGGATGCACATTCTCGGTTAT-TTCGCAGGATTGTTTTTCTATGTAACAGCACC 536
|||||||||||||||||||||| |||| || || | |||||||| ||||||| |||| ||
Sbjct 418 TCCGCTCGGATGCACATTCTCGCTTATGTTGGC-GCATTGTTTTACTATGTAGCAGCGCC 476
Query 537 TTTGTCACTCTGCTCCAATATTGCTCCAGAGGTAGCAAGATTTGTAGGAAATCAAGTGGC 596
|||||||||||||||||||||||||||||||||||||||||| ||||||| |||||||||
Sbjct 477 TTTGTCACTCTGCTCCAATATTGCTCCAGAGGTAGCAAGATTCGTAGGAAGTCAAGTGGC 536
Query 597 TGAGTTCATTGCTAATGGGAAAAGTCATACTTCAGCACCTGA-ATTAAGTTTAG-TAATG 654
|||||||||||||| ||||||||| |||| | || ||| |||| ||| | || |
Sbjct 537 TGAGTTCATTGCTAGTGGGAAAAGCCATA-GT---CA--TGATTTTAA-TTT-GTTATTA 588
Query 655 TGTATAAGTCCTTTGATGAAGCTTGGATCAGTCCAGTGGATTGGTGGAGCCATTTTTCTC 714
| |||||||||||||||||||||||||||| ||||||||||||||||||||||||||||
Sbjct 589 TCTATAAGTCCTTTGATGAAGCTTGGATCACTCCAGTGGATTGGTGGAGCCATTTTTCTT 648
Query 715 TGGGGATGGATACATCAGCGCCGCTGTCACGCCATTCTTGGATCGCTCCGGGAAAATCCG 774
||||||||||||||||| || ||||||||||||||||||||||| ||||||||| ||||
Sbjct 649 TGGGGATGGATACATCAACGTCGCTGTCACGCCATTCTTGGATCACTCCGGGAATATCCT 708
Query 775 AGCCAAGCGAAAGAGTACATAATTCCGTATGGGGATTGG-TTCAGGATGGTCTCTTCTCC 833
|| ||||| ||||||||||||||||| ||||| |||||| || | ||||||||| | |||
Sbjct 709 AGTCAAGCAAAAGAGTACATAATTCCATATGGAGATTGGTTTGA-GATGGTCTCATGTCC 767
Query 834 TCATTTCTTGGCAGAAATCGTTTTATATGC-GGGTTTACTGAT-TGCGAGTGGAGGAACA 891
|||||| | |||||||||||||||||| | |||||||| || | ||||||||||||||
Sbjct 768 GCATTTCCTAGCAGAAATCGTTTTATAT-CTAGGTTTACTTATAT-CGAGTGGAGGAACA 825
Query 892 GATATAACCATCTGGTTACTCTTTGGTTTCGTGGCTGCTAATCTGACATACGCGGCTGGA 951
||||||| ||||||||||||||||||||||||||||||||||||||||||||| ||||||
Sbjct 826 GATATAAGCATCTGGTTACTCTTTGGTTTCGTGGCTGCTAATCTGACATACGCAGCTGGA 885
Query 952 GAAACACACCGTTGGTATCTCCGAAAGTTTGAGGATTATCCGGCTAATCGGCATGCCATT 1011
||||| |||||||||||||||| |||||||||| |||||||||||| ||||||||| |||
Sbjct 886 GAAACGCACCGTTGGTATCTCCAAAAGTTTGAGAATTATCCGGCTAGTCGGCATGCTATT 945
Query 1012 TTCCCTCATGTCTACTAACTGGTTAAGA 1039
|||||||||||||||||| |||||||||
Sbjct 946 TTCCCTCATGTCTACTAAATGGTTAAGA 973
Score = 409 bits (221), Expect = 4e-113
Identities = 256/273 (94%), Gaps = 2/273 (1%)
Strand=Plus/Plus
Query 1 ATGGAGTTAGAGATTGTTTGGTTGGTTAGAGCCGCTTGGATCACCGTTTGGGTCGTCTCT 60
|||||| |||||||||| |||||||||| |||||||||||||||||||||| ||||||||
Sbjct 1 ATGGAGGTAGAGATTGTGTGGTTGGTTAAAGCCGCTTGGATCACCGTTTGGATCGTCTCT 60
Query 61 ATACTTCCTTTGGTGATTGCTTCGATACCCACTTCAAAGCTTAACTCTTTTCGTGAACTC 120
||||||||||||||||||||||| ||||| | ||||||||||||||||||||||||||||
Sbjct 61 ATACTTCCTTTGGTGATTGCTTCAATACCTAGTTCAAAGCTTAACTCTTTTCGTGAACTC 120
Query 121 GTTTTGAGTTTCGCTGGTAGAGGCAAGATTCTTCACCCTTCTTCTCAGAAGTTTACAGTT 180
|||||||||||||||||||||||||||||||||||||| || ||||||||||||||||||
Sbjct 121 GTTTTGAGTTTCGCTGGTAGAGGCAAGATTCTTCACCCATCCTCTCAGAAGTTTACAGTT 180
Query 181 CCACAGAAGTTCTTTGCTCACTTCTATGT-GATTGGAGTGGTGTGGACGACTCTTCTGCT 239
|| ||||||||||||| |||||||||||| | | |||||||||||||| ||||| |||||
Sbjct 181 CCTCAGAAGTTCTTTGGTCACTTCTATGTTG-TCGGAGTGGTGTGGACAACTCTCCTGCT 239
Query 240 TGCTGCAACTTGGATGTATGCTTGCGAAATGGC 272
||||||||||||||||||||||||| |||||||
Sbjct 240 TGCTGCAACTTGGATGTATGCTTGCAAAATGGC 272
> AT3G43840.1 | Symbols: | 3-oxo-5-alpha-steroid 4-dehydrogenase
family protein | chr3:15700352-15700855 FORWARD LENGTH=255
Length=255
Score = 209 bits (113), Expect = 4e-53
Identities = 126/132 (95%), Gaps = 2/132 (2%)
Strand=Plus/Plus
Query 899 CCATCTGGTTACTCTTTGGTTTCGTGGCTGCTAATCTGACATACGCGGCTGGAGAAACAC 958
|||||||||||||||||||||| ||||||||||||||||||||||||||||||||||| |
Sbjct 125 CCATCTGGTTACTCTTTGGTTTTGTGGCTGCTAATCTGACATACGCGGCTGGAGAAACGC 184
Query 959 ACCGTTGGTATCTC-CGAAAGTTTGAGGATTATCCGGCTAATCGGCATGCCATTTTCCCT 1017
|||||||||||||| | |||||||||| ||||||||||||||||||||||||||||||||
Sbjct 185 ACCGTTGGTATCTCGC-AAAGTTTGAGAATTATCCGGCTAATCGGCATGCCATTTTCCCT 243
Query 1018 CATGTCTACTAA 1029
|||||||||||
Sbjct 244 TATGTCTACTAA 255
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 56375212035
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5