BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg481725
Length=2591
Score E
Sequences producing significant alignments: (Bits) Value
AT2G27810.1 | Symbols: ATNAT12, NAT12 | nucleobase-ascorbate tr... 3666 0.0
> AT2G27810.1 | Symbols: ATNAT12, NAT12 | nucleobase-ascorbate
transporter 12 | chr2:11852080-11856291 FORWARD LENGTH=2691
Length=2691
Score = 3666 bits (1985), Expect = 0.0
Identities = 2313/2463 (94%), Gaps = 55/2463 (2%)
Strand=Plus/Plus
Query 160 aaaaaaaTGTCAAGCTCCGACCCGAAACCCGGACCTAAACCGGGTCCATGGCCTCCAGCT 219
||||||||||||||||||||||||||||||||||||||||||||||||||||| ||| ||
Sbjct 253 AAAAAAATGTCAAGCTCCGACCCGAAACCCGGACCTAAACCGGGTCCATGGCCACCAACT 312
Query 220 CCAGAATCCGCCGCAATGCCACCGTCTTCTTGGGCTaaaaaaaCCGGATTTCGTCCCAAA 279
||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
Sbjct 313 CCAGAATCCGCCGCAATGCCACCGTCTTCCTGGGCTAAAAAAACCGGATTTCGTCCCAAA 372
Query 280 TTCTCCGGTGAGACTACCGCCACTGATTC--C-TCCGGTCAGCTCTCATTACCGGTTAGA 336
||||||||||| ||||||||||||||||| | |||||||||||||||||||||||||||
Sbjct 373 TTCTCCGGTGAAACTACCGCCACTGATTCTTCTTCCGGTCAGCTCTCATTACCGGTTAGA 432
Query 337 GCTAGACAACAAGAAACCCAACCGGATCTTGAAGCTGGTCAAGCCAGACTCCGTCCTCCT 396
|||| ||||||||||||||||||||||||||||||||||||| |||||||||||||||||
Sbjct 433 GCTAAACAACAAGAAACCCAACCGGATCTTGAAGCTGGTCAAACCAGACTCCGTCCTCCT 492
Query 397 CCTGTCT-CCTCCTCCGCCGTAGCTAACGGTGAGACTGATAAAGATAAGAAAGAGAAACC 455
||| || |||| |||| ||| |||||||||||||||| || ||||||||||||
Sbjct 493 CCT-CCTGTCTCCGCCGCAGTAACTAACGGTGAGACTGACAAGGATAAGAAAGAG----- 546
Query 456 ACCACCACAAACAACAACAACACCACCTCCACCTCCTGGTTCTGTTGCTGTTCCGGTTAA 515
|| | | || ||||| ||||||||||||||||||||||||||||||||
Sbjct 547 --------AAGC--C-AC--CACCA---CCACCTCCTGGTTCTGTTGCTGTTCCGGTTAA 590
Query 516 GGATCAGCCGGTTAAGAGAAGGAGAGATTCTGATGGAGTTACTGGAAGATCAAATGGACC 575
|||||| ||||||||||||||||||||||||||||| ||| ||||||||||||||||||
Sbjct 591 GGATCAACCGGTTAAGAGAAGGAGAGATTCTGATGGGGTTGTTGGAAGATCAAATGGACC 650
Query 576 TGATGGAGCTAATGGGTCAGGTGACCCGGTTAGAAGACCGGGTCGTATTGAAGAGACAGT 635
||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
Sbjct 651 CGATGGAGCTAATGGGTCAGGTGACCCGGTTAGAAGACCGGGTCGTATTGAAGAAACAGT 710
Query 636 TGAGGTTTTGCCTCAGAGTATGGATGATGATTTAGTGGCTAGAAATTTGCATATGAAGTA 695
|||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
Sbjct 711 TGAGGTTTTGCCTCAGAGTATGGATGATGATTTAGTGGCTAGGAATTTGCATATGAAGTA 770
Query 696 TGGTCTCAGAGATACTCCTGGACTTGTTCCAATTGGGTTCTATGGTTTGCAACATTACCT 755
|||||| |||||||||||||||||||||||||||||||||||||||||||| ||||||||
Sbjct 771 TGGTCTTAGAGATACTCCTGGACTTGTTCCAATTGGGTTCTATGGTTTGCAGCATTACCT 830
Query 756 TTCAATGTTAGGATCATTGATTCTGGTTCCACTGGTTATTGTACCTGCAATGGGAGGTTC 815
||||||||| ||||||||||||||||||||||| |||||||| |||||||||||||||||
Sbjct 831 TTCAATGTTGGGATCATTGATTCTGGTTCCACTTGTTATTGTTCCTGCAATGGGAGGTTC 890
Query 816 CCACGAGGACATTGCAAATGTTGTATCGACTGTACTTTTTGTCTCTGGAATCACTACACT 875
|| ||||| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 891 ACATGAGGAGGTTGCAAATGTTGTATCGACTGTACTTTTTGTCTCTGGAATCACTACACT 950
Query 876 GTTGCATACTTCGTTTGGGTCGAGGCTTCCTTTGATTCAAGGACCTTCCTTTGTTTTTCT 935
|||||||||||||||||||||||||||||||||||||||||| ||||||||||| |||||
Sbjct 951 GTTGCATACTTCGTTTGGGTCGAGGCTTCCTTTGATTCAAGGTCCTTCCTTTGTCTTTCT 1010
Query 936 TGCTCCGGCTTTGGCTATAATAAACTCTCCGGAGTTTCAAGGTTTGAATGGAAATAATAA 995
||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
Sbjct 1011 GGCTCCGGCTTTGGCTATAATAAACTCTCCAGAGTTTCAAGGTTTGAATGGAAATAATAA 1070
Query 996 CTTCAAGCATATCATGAGGGAGCTACAAGGTGCAATCATAATTGGTTCAGCTTTTCAAGC 1055
|||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
Sbjct 1071 CTTCAAGCATATCATGAGGGAGCTGCAAGGTGCAATCATAATTGGTTCAGCTTTTCAAGC 1130
Query 1056 AGTGCTTGGATACAGTGGCCTAATGTCACTGATTTTGAGGTTGGTCAATCCAGTAGTTGT 1115
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1131 AGTGCTTGGATACAGTGGCCTAATGTCACTGATTTTGAGGTTGGTCAATCCAGTAGTTGT 1190
Query 1116 TGCTCCGACAATAGCTGCAGTTGGACTTTCTTTTTACAGTTATGGTTTCCCGCTTGTTGG 1175
||||||||| ||||||||||||||||||| |||||||| ||||||||||||||||||||
Sbjct 1191 TGCTCCGACGGTAGCTGCAGTTGGACTTTCCTTTTACAGCTATGGTTTCCCGCTTGTTGG 1250
Query 1176 TAAATGTCTTGAGATTGGTGTTGTGCAAATATTACTTGTGATCATCTTTGCTCTTTACCT 1235
||||||||||||||| ||||||||||| ||| ||||||||||||||||||||||||||||
Sbjct 1251 TAAATGTCTTGAGATCGGTGTTGTGCAGATACTACTTGTGATCATCTTTGCTCTTTACCT 1310
Query 1236 CCGGAAAATCTCAGTTTTAAGCCATCGTATTTTCCTGATTTATGCGGTTCCATTGAGTCT 1295
|||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
Sbjct 1311 CCGGAAAATCTCAGTTTTAAGCCATCGTATTTTCCTTATTTATGCGGTTCCATTGAGTCT 1370
Query 1296 TGCAATCACTTGGGCTGCTGCATTCCTTCTAACCGAAGCAGGAGCTTTCACCTACAAAGG 1355
|||||||||||||||||||||||||||| |||| ||| ||||||||| ||||||||||||
Sbjct 1371 TGCAATCACTTGGGCTGCTGCATTCCTTTTAACTGAAACAGGAGCTTACACCTACAAAGG 1430
Query 1356 TTGTGACCCAAACGTTCCTGTCTCAAATGTTGTATCGAGCCACTGCAGAAAATACATGAC 1415
|||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
Sbjct 1431 TTGTGACCCAAACGTTCCTGTCTCAAATGTTGTATCGACCCACTGCAGAAAATACATGAC 1490
Query 1416 CAGAATGAAGTACTGTCGGGTTGATACTTCTCATGCACTGAGTTCCGCCCCTTGGTTTAG 1475
|||||||||||||||||||||||||||||||||||| |||||||||||||| ||||||||
Sbjct 1491 CAGAATGAAGTACTGTCGGGTTGATACTTCTCATGCGCTGAGTTCCGCCCCATGGTTTAG 1550
Query 1476 GTTTCCTTACCCCTTGCAATGGGGTGTACCGATATTCAACTTGGAAA-TGGCTTTTGTTA 1534
||||||||||||||||||||||||||||||| ||||||||| ||||| ||||||||||||
Sbjct 1551 GTTTCCTTACCCCTTGCAATGGGGTGTACCGTTATTCAACT-GGAAAATGGCTTTTGTTA 1609
Query 1535 TGTGCGTAGTCTCCATAATTGCTTCAGTAGATTCGGTTGGTTCGTACCATGCTTCTTCTC 1594
|||| || |||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1610 TGTGTGTGGTCTCCGTAATTGCTTCAGTAGATTCGGTTGGTTCGTACCATGCTTCTTCTC 1669
Query 1595 TATTAGTAGCATCAAGGCCTCCAACTCGTGGTGTTGTGAGTCGGGCTATCGGTCTTGAAG 1654
||||||||||||| |||||||||||||||||||||||||||||||| |||||||||||||
Sbjct 1670 TATTAGTAGCATCGAGGCCTCCAACTCGTGGTGTTGTGAGTCGGGCAATCGGTCTTGAAG 1729
Query 1655 GTTTCACAAGTGTCTTAGCCGGTCTTTGGGGTATGGGTACTGGTTCAACCACTCTAACCG 1714
||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 1730 GTTTCACAAGTGTCTTAGCCGGTCTTTGGGGTACAGGTACTGGTTCAACCACTCTAACCG 1789
Query 1715 AAAACGTTCACACAATCGCTGTGACCAAAATGGGAAGCCGCAGAGTCGTTGAGTTAGGGG 1774
|||||||||||||||||||||||||||||||||||||||||||||| ||||||||||| |
Sbjct 1790 AAAACGTTCACACAATCGCTGTGACCAAAATGGGAAGCCGCAGAGTTGTTGAGTTAGGCG 1849
Query 1775 CATGTGTTTTGGTTATATTTTCCCTTCTTGGCAAGGTTGGAGGGTTTCTCGCATCAATTC 1834
| |||||||||||||||||||| ||| |||| ||||||||||||||||||||||||||||
Sbjct 1850 CGTGTGTTTTGGTTATATTTTCTCTTGTTGGTAAGGTTGGAGGGTTTCTCGCATCAATTC 1909
Query 1835 CGCAAGTCATGGTTGCCTCTCTTCTTTGCTTTATGTGGGCAATGTTTACGGCTTTAGGTC 1894
|||||||||||||||| |||||||| ||||| ||||||||||||||||||||||||||||
Sbjct 1910 CGCAAGTCATGGTTGCTTCTCTTCTGTGCTTCATGTGGGCAATGTTTACGGCTTTAGGTC 1969
Query 1895 TTTCCAACTTACGTTATAGTGAAGCTGGAAGCTCACGGAATATCATAATTGTCGGGTTGT 1954
|||||||||||||||||||||||||||||||||| ||||||||||||||||| |||||||
Sbjct 1970 TTTCCAACTTACGTTATAGTGAAGCTGGAAGCTCGCGGAATATCATAATTGTTGGGTTGT 2029
Query 1955 CGCTGTTCTTTTCTCTCTCTGTCCCAGCTTACTTCCAGCAATACGGCATTTCTCCAAACT 2014
|||||||||||||||||||||||||||||||||||||||| || ||||| ||||||||||
Sbjct 2030 CGCTGTTCTTTTCTCTCTCTGTCCCAGCTTACTTCCAGCAGTATGGCATATCTCCAAACT 2089
Query 2015 CGAATCTCTCTGTTCCAAGTTATTACCAACCTTACATTGTTTCTTCTCATGGACCCTTCA 2074
|||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
Sbjct 2090 CGAATCTCTCTGTTCCAAGTTATTACCAACCTTACATTGTTTCTTCACATGGACCCTTCA 2149
Query 2075 AAAGCCAATATAAAGGGGTGAACTATGTGATGAACACGCTCTTATCGATGAACATGGTGA 2134
|||||||||| |||||| ||||||| ||||||||||||||||| || |||| ||||||||
Sbjct 2150 AAAGCCAATACAAAGGGATGAACTACGTGATGAACACGCTCTTGTCAATGAGCATGGTGA 2209
Query 2135 TTGCGTTCATAATGGCTGTGATTTTGGACAACACAGTCCCGGGGAGCAAGCAAGAACGCG 2194
|||||||||| ||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 2210 TTGCGTTCATCATGGCTGTGATTTTGGACAACACGGTCCCGGGGAGCAAGCAAGAACGCG 2269
Query 2195 GAGTTTATGTTTGGTCTGACAGTGAGACTGCAACAAGAGAACCAGCACTTGCTAAAGACT 2254
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2270 GAGTTTATGTTTGGTCTGACAGTGAGACTGCAACAAGAGAACCAGCACTTGCTAAAGACT 2329
Query 2255 ATGAGCTGCCGTTTCGGGTGGGTCGGTTTTTCAGATGGGTCAAATGGGTGGGCATTTGAG 2314
|||| ||||||||||||||||| |||||||||||||||||||||||||||||||||||
Sbjct 2330 ATGAACTGCCGTTTCGGGTGGGGAGGTTTTTCAGATGGGTCAAATGGGTGGGCATTTGAT 2389
Query 2315 AAAACAACA-CA-A-TGGCAAAAAGTGGTAAATATGTCAGATGAGAGATCCCTCTTTTTC 2371
||||||||| || | |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2390 AAAACAACAACACAATGGCAAAAAGTGGTAAATATGTCAGATGAGAGATCCCTCTTTTTC 2449
Query 2372 TTTTATTTT-CA--TCTTCTCTGTTATTTTGATTAGTAGTC----A---------C--GG 2413
|||| |||| | || |||||||||||||||||||||||| | | ||
Sbjct 2450 TTTTCTTTTTCCCGTCGTCTCTGTTATTTTGATTAGTAGTCTCTCAATTTTATAACACGG 2509
Query 2414 TG-AAGCTTTTTCTTGGGTCGCAAGTTTGTCTATTGGAGAGTTTTTGGTTA---AAACAG 2469
|| ||||||||||||||||| |||||||||| ||||||||||||||||||| ||||||
Sbjct 2510 TGGAAGCTTTTTCTTGGGTCACAAGTTTGTCAATTGGAGAGTTTTTGGTTATTGAAACAG 2569
Query 2470 AGAATATGGAAGTCATGTTGTTGTAAGTTATATAGATTATGATCAAATGCTTTTGTAACT 2529
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2570 AGAATATGGAAGTCATGTTGTTGTAAGTTATATAGATTATGATCAAATGCTTTTGTAACT 2629
Query 2530 AGAGTTATTCAtttttt-tCACTATCTATATTTTTTCTTGTTTGGTAAATATATTGCGAA 2588
|||||||||| |||||| ||||||||||| || || |||||||| ||||| |||||||||
Sbjct 2630 AGAGTTATTC-TTTTTTCTCACTATCTATCTTATTCCTTGTTTGTTAAATCTATTGCGAA 2688
Query 2589 TTT 2591
|||
Sbjct 2689 TTT 2691
Score = 267 bits (144), Expect = 5e-70
Identities = 166/176 (94%), Gaps = 4/176 (2%)
Strand=Plus/Plus
Query 1 ctctctctctcgttttcacattcactgttacttcttcatagaacttctcactctctctt- 59
||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 39 CTCTCTCTCTCATTTTCACATTCACTGTTACTTCTTCATAGAACTTCTCACTCTCTCTCA 98
Query 60 cacacAACTGTACTCTCTCTGGAGATTTGAAGAAAACCCTAATTCCCAATTTTCTTTTGT 119
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 99 CACACAACTGTACTCTCTCTGGAGATTTGAAGAAAACCCTAATTCCCAATTTTCTTTTGT 158
Query 120 CGTTTTCCTTCTCCGGCGAG-GAGA-TTTTC-TAGATCTAAAGaaaaaaaTGTCAA 172
||||||||||||||| ||| |||| ||||| ||||||||||||||||| | ||||
Sbjct 159 CGTTTTCCTTCTCCGACGATTGAGAGTTTTCCTAGATCTAAAGAAAAAAGTTTCAA 214
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 128628041852
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5