BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg483774
Length=1919
Score E
Sequences producing significant alignments: (Bits) Value
AT2G46260.1 | Symbols: | BTB/POZ/Kelch-associated protein | ch... 3077 0.0
AT3G61600.1 | Symbols: ATPOB1, POB1 | POZ/BTB containin G-prote... 1550 0.0
> AT2G46260.1 | Symbols: | BTB/POZ/Kelch-associated protein |
chr2:18995968-18998633 FORWARD LENGTH=1999
Length=1999
Score = 3077 bits (1666), Expect = 0.0
Identities = 1839/1923 (96%), Gaps = 9/1923 (0%)
Strand=Plus/Plus
Query 1 AGAGAGAGACTTTTTTAGGGTTTCTTTGTTATTTTCGTTCCTC-AAAAAACCTTTTCGAA 59
||||| |||||||||||| |||||||||| ||||||||||||| ||||||||||||||||
Sbjct 1 AGAGAAAGACTTTTTTAGTGTTTCTTTGTCATTTTCGTTCCTCAAAAAAACCTTTTCGAA 60
Query 60 TTTTTGACCCTTATAGTTTTCCTGATTCTTCTGCTCGGTTCTCGCCGGAGAGAAG--ACG 117
|||||||||||| ||| |||||||||||||||||||||||||||||||||||| |||
Sbjct 61 --TTTGACCCTTATTGTTATCCTGATTCTTCTGCTCGGTTCTCGCCGGAGAGAAGACACG 118
Query 118 GAGAAGAGGGTTTTCGAAAAGGAGGATGAGAGGTTCCAATAACACCGATCTATTTGACCC 177
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 119 GAGAAGAGGGTTTTCGAAAAGGAGGATGAGAGGTTCCAATAACACCGATCTATTTGACCC 178
Query 178 TAAGACGGAGATGGATTCCAATTTCTCCCGCCATGGTTCTTCCTCCGAAGGTGATTTTGG 237
||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||
Sbjct 179 TAAGACGGAGATGGATTCCAATTTCTCTCGCCATGGTTCTTCCTCCGAAGGTGATTTTGG 238
Query 238 TTTCGCTTTCAATGACAGTAACTTCTCCGATCGTTTGCTCCGGATCGAGATCTTGGGTGG 297
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 239 TTTCGCTTTCAATGACAGTAACTTCTCCGATCGTTTGCTCCGGATCGAGATCTTGGGTGG 298
Query 298 GCCTTCGGATTCTAGGTCTGATGGTGAAGGATGTACGAGTATTGCCGATTGGGCTCGTCA 357
||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
Sbjct 299 GCCTTCGGATTCTAGGTCTGATGCTGAAGGATGTACGAGTATTGCCGATTGGGCTCGTCA 358
Query 358 CCGCAAGAGAAGAAGAGAGGACAACAAGAAGGACAATGGTGTTGCGATTTCAGACATTGT 417
|||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 359 TCGCAAGAGGAGAAGAGAGGACAACAAGAAGGACAATGGTGTTGCGATTTCAGACATTGT 418
Query 418 GGCATGTGCTGAAGAACAGATTTTAACCGATAACAACCAACCTGATATGGATGATGGTCC 477
|||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
Sbjct 419 GGCATGTGCTGAAGAACAGATTTTAACCGATAACAACCAACCTGATATGGATGATGCTCC 478
Query 478 TGGTGGTGATAATCTTGACGATGAAGGAGAGGCTATGGTCGAAGAGGCTTTATCAGGTGA 537
||||||||||||||||||||||||||||||||| ||||| ||||||||||||||||||||
Sbjct 479 TGGTGGTGATAATCTTGACGATGAAGGAGAGGCAATGGTTGAAGAGGCTTTATCAGGTGA 538
Query 538 TGACGATGCATCTAGCGAGCCAAATTGGGGTATTGATTGTTCTACTGTTGTTAGAGTTAA 597
||| ||||||||||| |||||||| |||||||||||||||||||||||||||||||||||
Sbjct 539 TGATGATGCATCTAGTGAGCCAAACTGGGGTATTGATTGTTCTACTGTTGTTAGAGTTAA 598
Query 598 AGAACTGCATATTAGTTCCCCTATCTTGGCTGCTAAAAGCCCCTTTTTCTACAAGTTGTT 657
|||||||||||||||||| ||||||||||| || ||||||||||||||||||||| ||||
Sbjct 599 AGAACTGCATATTAGTTCTCCTATCTTGGCCGCAAAAAGCCCCTTTTTCTACAAGCTGTT 658
Query 658 TTCTAATGGAATGAGGGAATCAGAACAAAGACATGTAACCCTTAGAATTAGTGCACAAGA 717
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 659 TTCTAATGGAATGAGGGAATCAGAACAAAGACATGTAACCCTTAGAATTAGTGCACAAGA 718
Query 718 GGAAGGTGCTTTGATGGAGCTTTTAAACTTTATGTATAGCAACTCTCTAAGTGTCACAAC 777
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 719 GGAAGGTGCTTTGATGGAGCTTTTAAACTTTATGTATAGCAACTCTCTAAGTGTCACAAC 778
Query 778 AGCACCTGCTTTATTAGATGTGCTTATGGCTGCTGACAAGTTTGAGGTTGCTTCCTGCAT 837
|||||| |||||||||||||| ||||||||||||||||||||||||||||||||||| ||
Sbjct 779 AGCACCCGCTTTATTAGATGTTCTTATGGCTGCTGACAAGTTTGAGGTTGCTTCCTGTAT 838
Query 838 GAGGTATTGTAGTAGACTACTGCGCAATATGCCCATGACCCCTGATTCCGCTTTGCTCTA 897
|||||||||||||||||||||||| ||||||||||||||||||||||| |||||||||||
Sbjct 839 GAGGTATTGTAGTAGACTACTGCGAAATATGCCCATGACCCCTGATTCTGCTTTGCTCTA 898
Query 898 TCTCGAGCTTCCCTCTAGTGTTTTAATGGCTGAAGCAGTCCAACCTCTAACCGATGCAGC 957
||| ||||| ||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 899 TCTTGAGCTGCCCTCTAGTGTTTTAATGGCTGAAGCAGTGCAACCTCTAACCGATGCAGC 958
Query 958 AAAGCAGTTCCTTGCCTCCCGCTACAAGGATATTACCAAATTTCATGATGAGGTTATGGC 1017
|||||||||||||||||| |||||||||||||||||||| ||||||||||||||||||||
Sbjct 959 AAAGCAGTTCCTTGCCTCGCGCTACAAGGATATTACCAAGTTTCATGATGAGGTTATGGC 1018
Query 1018 CTTACCATTGGCTGGAATTGAGGCGATACTGTCGAGCGATGATCTCCAAATTGCTTCTGA 1077
|||||||||||||||||| ||||||||||| |||||||||||||||||||||||||||||
Sbjct 1019 CTTACCATTGGCTGGAATAGAGGCGATACTATCGAGCGATGATCTCCAAATTGCTTCTGA 1078
Query 1078 GGATGCTGTTTATGATTTTGTTTTGAAATGGGCAAGGGGACAGTACAGTTCATTGGAAGA 1137
||||||||||||||||||||| ||||||||||||||||||||||| ||||||||||||||
Sbjct 1079 GGATGCTGTTTATGATTTTGTCTTGAAATGGGCAAGGGGACAGTATAGTTCATTGGAAGA 1138
Query 1138 CCGTAGAGAGATTCTGGGTTCACGCCTTGCGCTCTACATCCGCTTCCCATACATGACATG 1197
||||||||||||||||||||||||||||| |||||||||||||||||||||||||| ||
Sbjct 1139 TCGTAGAGAGATTCTGGGTTCACGCCTTGCACTCTACATCCGCTTCCCATACATGACGTG 1198
Query 1198 TCGAAAGCTGAAAAAGGTACTAACGTGTAGCGATTTTGAGCATGAAGTAGCATCAAAGCA 1257
||||||||||||||||||||||||||| || |||||||||||||||||||||||||||||
Sbjct 1199 TCGAAAGCTGAAAAAGGTACTAACGTGCAGTGATTTTGAGCATGAAGTAGCATCAAAGCA 1258
Query 1258 AGTATTAGAAGCGCTCTTCTTCAAAGCAGAAGCCCCACACAGGCAACGCATCCTCGCAGC 1317
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1259 AGTATTAGAAGCGCTCTTCTTCAAAGCAGAAGCCCCACACAGGCAACGCATCCTCGCAGC 1318
Query 1318 TGAAGGATCAGACTCCTTGAATCGCCGTTTCATAGAGAGGGCTTACAAATACAGACCCGT 1377
||||||||| |||||| |||| ||||||||||||||||||||||||||||||||||||||
Sbjct 1319 TGAAGGATCGGACTCCATGAACCGCCGTTTCATAGAGAGGGCTTACAAATACAGACCCGT 1378
Query 1378 AAAAGTGGTGGAGTTTGAGCTTCCTCGGCCGCAATGTGTAGTGTACTTGGACTTGAAGCG 1437
||||||||||||||||||||||||||| |||||||||||||||||| |||||||||||||
Sbjct 1379 AAAAGTGGTGGAGTTTGAGCTTCCTCGCCCGCAATGTGTAGTGTACCTGGACTTGAAGCG 1438
Query 1438 AGAGGAATGCGCAGGATTGTTCCCTTCGGGAAGAGTCTATTCTCAGGCCTTCCACTTAGG 1497
|||||||||||||||| | |||||||| |||||||||||||||||||| |||||||||||
Sbjct 1439 AGAGGAATGCGCAGGACTATTCCCTTCAGGAAGAGTCTATTCTCAGGCTTTCCACTTAGG 1498
Query 1498 AGGTCAAGGGTTCTTTCTCTCGGCACACTGCAACATGGACCAGCAAAGCTCGTTCCACTG 1557
||||||||| ||||| ||||| ||||||||||||||||||||||||||||||||||||||
Sbjct 1499 AGGTCAAGGTTTCTTCCTCTCAGCACACTGCAACATGGACCAGCAAAGCTCGTTCCACTG 1558
Query 1558 CTTTGGGCTATTCCTCGGGATGCAAGAAAAAGGAGCTGTGAGTTTTGGTGTGGACTACGA 1617
|||||||||||||||||| |||||||| ||||| ||||||||||||||||||||||||||
Sbjct 1559 CTTTGGGCTATTCCTCGGAATGCAAGAGAAAGGGGCTGTGAGTTTTGGTGTGGACTACGA 1618
Query 1618 ATTCGCGGCAAGAGATAAAAA-ATCATCCGAAGAATACGTAAGCAAATACAAAGGAAACT 1676
||| |||||||||||||||| | || |||||||||||||||||||||||||||||||
Sbjct 1619 ATTTGCGGCAAGAGATAAAAGTA-CAAAGGAAGAATACGTAAGCAAATACAAAGGAAACT 1677
Query 1677 ACACATTCACAGGGGGGAAAGCAGTTGGTTACAGAAATCTTTTTGGGATTCCGTGGACTT 1736
|||||||||||||||||||||| |||||||||||||| ||||| |||||||| |||||||
Sbjct 1678 ACACATTCACAGGGGGGAAAGCGGTTGGTTACAGAAACCTTTTCGGGATTCCATGGACTT 1737
Query 1737 CGTTCATTGCTGATGACAGTCAGCATTTCATCAATGGCATTCTCCATCTCAGAGCCGAGC 1796
||||||| || || |||||||| |||||||||||||||||||||||||||||||||||||
Sbjct 1738 CGTTCATAGCCGAGGACAGTCAACATTTCATCAATGGCATTCTCCATCTCAGAGCCGAGC 1797
Query 1797 TCACCATTAAGCGTTCCTCAGACCTGCACTGACTGTTTtctctctctctctttctctACA 1856
|||||||||| |||||||||||||||||||||||||||||| ||||||||| ||||||
Sbjct 1798 TCACCATTAAACGTTCCTCAGACCTGCACTGACTGTTTTCT-TCTCTCTCTCTCTCTATG 1856
Query 1857 TGGCCACGTTTAAGTTGTATTATCTCGTTATGTAGCCAGCTCTAACCACGAGTGTCTTTT 1916
| | ||| |||||||||||| ||||||||||||||| ||||||||||||||||||| |||
Sbjct 1857 TCGTCACATTTAAGTTGTATAATCTCGTTATGTAGC-AGCTCTAACCACGAGTGTCCTTT 1915
Query 1917 GTA 1919
|||
Sbjct 1916 GTA 1918
> AT3G61600.1 | Symbols: ATPOB1, POB1 | POZ/BTB containin G-protein
1 | chr3:22795579-22798320 FORWARD LENGTH=2178
Length=2178
Score = 1550 bits (839), Expect = 0.0
Identities = 1364/1616 (84%), Gaps = 42/1616 (3%)
Strand=Plus/Plus
Query 209 CATGGTTCTTCCTCCGAAGGTGATTTTGGTTTCGCTTTCAATGACAGTAACTTCTCCGAT 268
||||||||||||||||| || |||||||| |||||||| || |||||||||||||| |||
Sbjct 195 CATGGTTCTTCCTCCGACGGCGATTTTGGATTCGCTTTTAACGACAGTAACTTCTCTGAT 254
Query 269 CGTTTGCTCCGGATCGAGATCTTGGGTGGGCCTTCGGATTCTAGGTCTGATGGTGAAGGA 328
|||||||| |||||||||||| ||||||| |||||||||||| ||||||| | ||||||
Sbjct 255 CGTTTGCTTCGGATCGAGATCATGGGTGGACCTTCGGATTCTCGGTCTGAAGTTGAAGGG 314
Query 329 TGTACGAGTATTGCCGATTGGGCTCGTCACCGCAAGAGAAGAAGAGAGGACAACAAGAAG 388
||||||||||| || |||||||||||||| ||||||||||||||||| || | |||||||
Sbjct 315 TGTACGAGTATCGCTGATTGGGCTCGTCATCGCAAGAGAAGAAGAGAAGATATCAAGAAG 374
Query 389 GACA-ATGGTGTTGCGATTTCAGACATTGTGGCATGTGCTGAAGAACAGATTTTAACCGA 447
|| | |||||| ||||||||||||||||||||||| |||| || ||||||||||| ||
Sbjct 375 GA-ATCTGGTGTCACGATTTCAGACATTGTGGCATGTCCTGAGGAGCAGATTTTAACTGA 433
Query 448 TAACAACCAACCTGATATGGAT-GATGGTCCTGGTGGTGATAATCTTGACGATG-A--AG 503
| || |||||||| |||||| ||| ||||||||||||| |||| ||| |||| | ||
Sbjct 434 T--GAA-CAACCTGACATGGATGGAT-GTCCTGGTGGTGAGAATCCTGATGATGAAGGAG 489
Query 504 GAGAGGCTATGGTCGAAGAGGCTTTATCAGGTGATGACGATG---CATCTAGCGAGCCAA 560
||||||| ||||| ||||| ||||||||||||||||| || | | ||||| |||||||
Sbjct 490 GAGAGGCAATGGTTGAAGAAGCTTTATCAGGTGATGAAGAGGAAACGTCTAGTGAGCCAA 549
Query 561 ATTGGGGTATTGATTGTTCTACTGTTGTTAGAGTTAAAGAACTGCATATTAGTTCCCCTA 620
| ||||| || ||||||||||| |||||||| ||||||||||| ||||||||||| ||||
Sbjct 550 ACTGGGGAATGGATTGTTCTACAGTTGTTAGGGTTAAAGAACTTCATATTAGTTCTCCTA 609
Query 621 TCTTGGCTGCTAAAAGCCCCTTT-TTCTACAAGTTGTTTTCTAATGGAATGAGGGAATCA 679
| || ||||| ||||| |||||| || ||||||||||| || |||||||||||||||||
Sbjct 610 TTTTAGCTGCCAAAAG-CCCTTTCTTTTACAAGTTGTTCTCCAATGGAATGAGGGAATCT 668
Query 680 GAACAAAGACATGTAACCCTTAGAATTAGTGCA-CAAGAGGAAGGTGCTTTGATGGAGCT 738
|| ||||| ||||| |||||||| |||| |||| | |||||||| |||||||||||||||
Sbjct 669 GAGCAAAGGCATGTCACCCTTAGGATTAATGCATC-AGAGGAAGCTGCTTTGATGGAGCT 727
Query 739 TTTAAACTTTATGTATAGCAACT-CTCTAAGTGTCACAACAGCACCTGCTTTATTAGATG 797
||||||||||||||||||||| | | || |||||| ||||||||||| ||||||||||
Sbjct 728 TTTAAACTTTATGTATAGCAA-TGCGGTATCTGTCACCACAGCACCTGCCTTATTAGATG 786
Query 798 TGCTT-ATGGCTGCTGACAAGTTTGAGGTTGCTTCCTGCATGAGGTATTGTAGTAGACTA 856
|| || ||||||||||| |||||||| ||||| || || |||||||| || ||||||||
Sbjct 787 TG-TTGATGGCTGCTGATAAGTTTGAAGTTGCCTCTTGTATGAGGTACTGCAGTAGACTT 845
Query 857 CTGCGCAATATGCCCATGACCCCTGATTCCGCTTTGCTCTATCTCGAGCTTCCCTCTAGT 916
|| || |||||||| ||||| || || || || ||||||||||||||||||||||||||
Sbjct 846 CTCCGTAATATGCCTATGACTCCAGAGTCTGCCCTGCTCTATCTCGAGCTTCCCTCTAGT 905
Query 917 GTTTTAATGGCTGAAGCAGTCCAACCTCTAACCGATGCAGCAAAGCAGTTCCTTGCCTCC 976
||| ||||||| |||| || || ||| |||| ||||| ||||| ||||||||||| ||
Sbjct 906 GTTCTAATGGCCAAAGCTGTTCAGCCTTTAACTGATGCTGCAAAACAGTTCCTTGCAGCC 965
Query 977 CGCTACAAGGATATTACCAAATTTCATGATGAGGTTATGGC-CTTACCATTGGCTGGAAT 1035
|||||||||||||||||||| |||||||| ||||| ||| | || ||| |||||||||||
Sbjct 966 CGCTACAAGGATATTACCAAGTTTCATGAGGAGGTAATGTCTCT-ACCTTTGGCTGGAAT 1024
Query 1036 TGAGGCGATACTGTCGAGCGATGATCTCCAAATTGCTTCTGAGGATGCTGTTTATGATTT 1095
|||||| || || || |||||||| ||||||||||| || |||||||| |||||||||||
Sbjct 1025 TGAGGCAATTCTATCAAGCGATGAACTCCAAATTGCATCAGAGGATGCAGTTTATGATTT 1084
Query 1096 TGTTTTGAAATGGGCAAGGG-GACAGTACAGTTCATTGGAAGACCGTAGAGAGATTCT-G 1153
| | ||||| |||||||||| | || ||| || |||||||| || ||||||||||| |
Sbjct 1085 TATCTTGAAGTGGGCAAGGGCG-CAATACCCTTGTTTGGAAGAGCGAAGAGAGATTCTCG 1143
Query 1154 GGTTCACGCCTTGCGCTCTACATCCGCTTCCCATACATGACATGTCGAAAGCTGAA-AAA 1212
||| |||||||||| |||| |||||||||||||| ||||||||| ||||||||||| |||
Sbjct 1144 GGT-CACGCCTTGCACTCTCCATCCGCTTCCCATTCATGACATGCCGAAAGCTGAAGAAA 1202
Query 1213 GGTACTAACGTGTAGCGATTTTGAGCATGAAGTAGCATCAAAGCAAGTAT-TAGAAGCGC 1271
| | || || || || || |||||||||||| |||||||||||| || | ||||||| |
Sbjct 1203 G-TGCTGACTTGCAGTGACTTTGAGCATGAAATAGCATCAAAGCTTGT-TCTAGAAGCTC 1260
Query 1272 TCTTCTTCAAAGCAGAAGCCCCACACAGGCAACGCATCCTCGCAGCTGAAGGATCAG-AC 1330
| |||||||||||||||||||||||||| ||||| | ||| || | |||| ||| | |
Sbjct 1261 TTTTCTTCAAAGCAGAAGCCCCACACAGACAACGTAGCCTAGCCTCCGAAGAATCTGCA- 1319
Query 1331 TCCTTGAATCGCCGTTTCATAGAGAGGGCTTACAAATACAGACCCGTAAAAGTGGTGGAG 1390
||| |||| ||||| | ||||||||||||||||||||||||||||| |||||||| |||
Sbjct 1320 TCCCTGAACCGCCGCCTGATAGAGAGGGCTTACAAATACAGACCCGTCAAAGTGGTCGAG 1379
Query 1391 TTTGAGCTTCCTCGGCCGCAATGTGTAGTGTACTTGGACTTGAAGCGAGAGGAATGCGCA 1450
|||||||||||| | ||||| |||||||| ||| | |||||||| |||| ||||| |
Sbjct 1380 TTTGAGCTTCCTAGACCGCAGTGTGTAGTCTACCTAGACTTGAAAAGAGAAGAATGTGGG 1439
Query 1451 GGATTGTTCCCTTCGGGAAGAGTCTATTCTCAGGCCTTCCACTTAGGAGGTCAAGGGTTC 1510
||| ||||||| ||||| ||||| ||||| |||||||| ||||| ||||||||||||||
Sbjct 1440 GGACTGTTCCCGTCGGGTAGAGTGTATTCGCAGGCCTTTCACTTGGGAGGTCAAGGGTT- 1498
Query 1511 TTTC-TCTCGGCACACTGCAACATGGACCAGCAAAGCTCGTTCCACTGCTTT-GGGCTAT 1568
|||| | || || ||||||||||||||||| || |||||||||||||| ||| ||||| |
Sbjct 1499 TTTCCTGTCAGCTCACTGCAACATGGACCAACAGAGCTCGTTCCACTG-TTTCGGGCTGT 1557
Query 1569 TCCTCGGGATGCAAGAAAAAGGAG-CTGTGAGTTTTGGTGTGGACTACGAATTCGCGGCA 1627
|||| |||||||| || ||||| | | |||||||| || |||||||| |||||| |||||
Sbjct 1558 TCCTAGGGATGCAGGAGAAAGG-GTCGGTGAGTTTCGGAGTGGACTATGAATTCTCGGCA 1616
Query 1628 AGAGATAAAAAATCATCCGAAGAATACGTAAGCAAATACAAAGGAAACTACACATTCACA 1687
|| | | ||| | | || || | | |||||||||||||||| |||||||||||||||
Sbjct 1617 AG-G-TCAAAGCCCG-CAGAGGATTTCATAAGCAAATACAAAGGGAACTACACATTCACA 1673
Query 1688 GGGGGGAAAGCAGTTGGTTACAGAAATCTTTTTGGGATTCCGTGGACTTCGTTCATTGCT 1747
|| ||||||||||| ||||||||||| || |||||| | || ||||| || || || ||
Sbjct 1674 GGAGGGAAAGCAGTAGGTTACAGAAACCTGTTTGGGGTCCCATGGACGTCTTTTATAGCG 1733
Query 1748 GATGACAGTCAGCATTTCATCAATGGCATTCTCCATCTCAGAGCCGAGCTCACCAT 1803
|| || ||||| | ||||||||||||||||||||||||||||| ||||| |||||
Sbjct 1734 GAAGATAGTCAATACTTCATCAATGGCATTCTCCATCTCAGAGCAGAGCTTACCAT 1789
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 95029631685
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5