BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg484476
Length=2248
Score E
Sequences producing significant alignments: (Bits) Value
AT3G26910.2 | Symbols: | hydroxyproline-rich glycoprotein fami... 3469 0.0
AT5G41100.1 | Symbols: | FUNCTIONS IN: molecular_function unkn... 547 1e-154
> AT3G26910.2 | Symbols: | hydroxyproline-rich glycoprotein family
protein | chr3:9915128-9918743 REVERSE LENGTH=2253
Length=2253
Score = 3469 bits (1878), Expect = 0.0
Identities = 2146/2270 (95%), Gaps = 39/2270 (2%)
Strand=Plus/Plus
Query 1 AAAGAAACtttttttttttGGTCTTCCTTATTCTTTCTTCAATGTCGTTGAACATATCTG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1 AAAGAAACTTTTTTTTTTTGGTCTTCCTTATTCTTTCTTCAATGTCGTTGAACATATCTG 60
Query 61 ACGATACTTGAATCTCA------C-TCT-TTC--TTCTCTG-ATTTAGTCTCAATTTCAT 109
||||||||||||||||| | ||| ||| ||||||| ||||||| |||||||||
Sbjct 61 ACGATACTTGAATCTCACTCTTTCTTCTCTTCTATTCTCTGTTTTTAGTCACAATTTCAT 120
Query 110 CT-TATCTCTTCTCACTTTTG-TTCTGAGAAATTTCGATCCCAAGCTTTTTCTCAGAGAA 167
|| |||||| ||||||||||| || |||||| |||||||||||||| |||||||||||||
Sbjct 121 CTATATCTCCTCTCACTTTTGTTTTTGAGAATTTTCGATCCCAAGC-TTTTCTCAGAGAA 179
Query 168 TTTTGACTGGAAAATTTCGTAATTAGGGTTTTTGAGATTTGA--CATTATTCTACGATGA 225
|||||||||| |||||||||||||||||||||| ||||||| ||||||||||||||||
Sbjct 180 TTTTGACTGG-AAATTTCGTAATTAGGGTTTTT--GATTTGAGGCATTATTCTACGATGA 236
Query 226 AAGCTTCGATTGAGAAGTTAAGGAGATTAACATCGCATTCACATAAGGTTGATGTGAAGG 285
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 237 AAGCTTCGATTGAGAAGTTAAGGAGATTAACATCGCATTCACATAAGGTTGATGTGAAGG 296
Query 286 AGAAAGGAGAGGTCATGGCTACAACACAAATTGACGAACTCGATCGAGCCGGGAAGGATA 345
|||||||||| |||||||||||||||||||| ||||||||||||||||||||||||||||
Sbjct 297 AGAAAGGAGATGTCATGGCTACAACACAAATCGACGAACTCGATCGAGCCGGGAAGGATA 356
Query 346 TGCAAGATATGAGAGACTGCTACGATAGACTACTCTCCGCAGCTGCTGCCACGGCTAATA 405
|||||||||||||||| || ||||||||||||||| | ||||||||||||||||| ||||
Sbjct 357 TGCAAGATATGAGAGAATGTTACGATAGACTACTCGCTGCAGCTGCTGCCACGGCAAATA 416
Query 406 GCGCTTATGAGTTCTCTGAGTCTTTGGGAGAAATGGGTTCTTGTTTGGAGCAAATCGCGC 465
|||| ||||||||||||||||| |||||||||||||||||||||||||||||||||||||
Sbjct 417 GCGCATATGAGTTCTCTGAGTCGTTGGGAGAAATGGGTTCTTGTTTGGAGCAAATCGCGC 476
Query 466 CTCATAACGACGAAGAAAGCAGTAGAATCTTGTTTATGTTGGGTAAAGTTCAGAGTGAGC 525
|||||||||||||||| |||||||||||||||||||||||||||||||| ||||||||||
Sbjct 477 CTCATAACGACGAAGAGAGCAGTAGAATCTTGTTTATGTTGGGTAAAGTACAGAGTGAGC 536
Query 526 TTCAAAGACTTCTTGACACATATCGTAGTCATATATTCAAAACCATTACATCCCCATCAG 585
|||||||||||||||||||||||||||||||||||||| |||| ||||||||||| || |
Sbjct 537 TTCAAAGACTTCTTGACACATATCGTAGTCATATATTCGAAACTATTACATCCCCGTCCG 596
Query 586 AGGCGCTTCTCAAGGACCTCAGA-ACTGTTGAGGATATGAAGCAACAATGCGACGAGAAG 644
||||||||||||||||||||||| | ||||||||||||||||||||||||||||| ||||
Sbjct 597 AGGCGCTTCTCAAGGACCTCAGATA-TGTTGAGGATATGAAGCAACAATGCGACGGGAAG 655
Query 645 AGGAATGTGTATGAGATGTCGCTAGTAAAAGATAAAGGAAGGCTTAAAAGCAGTAAAGGA 704
|||||||||||||||||||||||||| ||||| |||||||||| ||||||||||||||||
Sbjct 656 AGGAATGTGTATGAGATGTCGCTAGTGAAAGAGAAAGGAAGGCCTAAAAGCAGTAAAGGA 715
Query 705 GAGAGGCATATTCCTCCCGAGTCTCGACCTGCTTACAGTGAGTTTCATGACGAAGCAACA 764
||||| ||||||||||| |||||||||||||||||||||||||||||||| ||||| |||
Sbjct 716 GAGAGACATATTCCTCCTGAGTCTCGACCTGCTTACAGTGAGTTTCATGATGAAGCCACA 775
Query 765 ATGTGCATTTTTCGATTGAAATCTCTTAAAGAAGGACAAGCTCGTAGTCTCCTTACACAA 824
||||||||||||||||||||||| ||||||||||||||||||||||||||||| | ||||
Sbjct 776 ATGTGCATTTTTCGATTGAAATCGCTTAAAGAAGGACAAGCTCGTAGTCTCCTAATACAA 835
Query 825 GCAGTCCGTCACCACACTGCTCAGATGCGTTTGTTCCACACTGGACTGAAATCTCTCGAG 884
||||||||||||||||||||||||||||||||||| ||||||||||||||||| ||||||
Sbjct 836 GCAGTCCGTCACCACACTGCTCAGATGCGTTTGTTTCACACTGGACTGAAATCGCTCGAG 895
Query 885 GCAGTTGAGCGTCATGTAAAAGTTGCTGCAGAGAAACAACACATTGACTGTGATCTATCT 944
|||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 896 GCAGTTGAGCGTCATGTAAAAGTTGCTGTAGAGAAACAACACATTGACTGTGATCTATCT 955
Query 945 GTTCATGGGAACGAGGTGGAAGCTAGCGAGTatgatgatgatgatgatAGCCGATATATG 1004
||||||||||||||| |||||||||||||| ||||||||||||||| ||||||| |||
Sbjct 956 GTTCATGGGAACGAGATGGAAGCTAGCGAG---GATGATGATGATGATGGCCGATACATG 1012
Query 1005 AACAGAGAAGGAGAACTCAGTTTTGATTACAGAACAAATGACCAGAATGTAGAAGCTGCT 1064
|| |||||||||||||||||||||||||||||||||||||| ||||| ||||||||| ||
Sbjct 1013 AATAGAGAAGGAGAACTCAGTTTTGATTACAGAACAAATGAGCAGAAGGTAGAAGCTTCT 1072
Query 1065 TCTCTCTCTACACCATGGGGCTCAAAGATGGATGATACAGACCTCTCGTTTCCTCGCCCT 1124
||||||||||||||||||| | ||||||||||||||||||||||||||||||||||||||
Sbjct 1073 TCTCTCTCTACACCATGGGCCACAAAGATGGATGATACAGACCTCTCGTTTCCTCGCCCT 1132
Query 1125 TCTACAACACGACCAGCAGCGGTAAATGCTGATCATAGAGAAGAATATCCAGTTTCAACC 1184
||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1133 TCTACAACAAGACCAGCAGCGGTAAATGCTGATCATAGAGAAGAATATCCAGTTTCAACC 1192
Query 1185 CGCGATAAGTATTTGAGCAGCCATTCAGCTCCGTTGTTCCCAGAAAAGAAACCTGATTTA 1244
||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||
Sbjct 1193 CGCGATAAGTATTTGAGCAGCCATTCAGCTCCGTTGTTCCCAGAAAAGAAACCTGATGTA 1252
Query 1245 TCAGAGAGGTTGAGACAGGTGGTGAATCCATCTTTTAATGCCTACGTATTACCAACACCA 1304
||||||||||||||||||| | ||||||||||||||||||||||||||||||||||||
Sbjct 1253 TCAGAGAGGTTGAGACAGGCG---AATCCATCTTTTAATGCCTACGTATTACCAACACCA 1309
Query 1305 AACGATTCAAGGTACTCGAAACCGGTTTCCCAAGCTTTAAATCCGAGGCCAGCAAACCAC 1364
|| |||||||||||||| ||||||||||||||||| ||||||||||||||| ||||||||
Sbjct 1310 AATGATTCAAGGTACTCAAAACCGGTTTCCCAAGCATTAAATCCGAGGCCAACAAACCAC 1369
Query 1365 AGTGCTGGAAACATATGGCATTCATCTCCGTTAGAGCCGATAAAAAGCGGGAAAGATGGG 1424
||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1370 AGTGCCGGAAACATATGGCATTCATCTCCGTTAGAGCCGATAAAAAGCGGGAAAGATGGG 1429
Query 1425 AAAGACGCGGAAAACAACAGCTTCTACGGCCGCCTCCCTCGGCCTTCTACAACAGACACG 1484
|||||||| |||| ||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1430 AAAGACGCCGAAAGCAACAGCTTCTACGGCCGCCTCCCTCGGCCTTCTACAACAGACACG 1489
Query 1485 CATCATCATCATCAGCAGCAAGCAGCAGGAAGACATGCATTTTCCGGACCTCTCAAACCG 1544
||||||||||| |||||||||||||||||||||||||||||| |||||||||| ||||
Sbjct 1490 CATCATCATCA---GCAGCAAGCAGCAGGAAGACATGCATTTTCTGGACCTCTCAGACCG 1546
Query 1545 TCCTCAACAAAACCTGTCACCACGGCGGACAGTTACTCAGGCGCGTTTTGTCCTCTGCCG 1604
|||||||||||||| |||||| ||| |||||||| |||||||| |||||||||||||||
Sbjct 1547 TCCTCAACAAAACCCATCACCATGGCTGACAGTTATTCAGGCGCTTTTTGTCCTCTGCCG 1606
Query 1605 ACTCCTCCAATACTCC---CTCATCCC-CATTCATCAGCTTCTCCAAGAGTCTCCCCTAC 1660
||||||||| |||||| |||| ||| ||||||||| ||||||||||||||||||||||
Sbjct 1607 ACTCCTCCAGTACTCCAATCTCA-CCCTCATTCATCATCTTCTCCAAGAGTCTCCCCTAC 1665
Query 1661 AGCTTCACCTCCTCCTGCTTCTTCCCCGAGACTCAACGAGCTTCACGAGCTTCCAAGACC 1720
|||||||||||||||||||||||||| || |||||||||||||||||||||||||||||
Sbjct 1666 CGCTTCACCTCCTCCTGCTTCTTCCCCAAGGCTCAACGAGCTTCACGAGCTTCCAAGACC 1725
Query 1721 CCCAGGCCACTTTGCACCACCGCCAAGACGAGCCAAGTCCCCTGGTCTGGTTGGTCACTC 1780
||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
Sbjct 1726 CCCAGGCCACTTTGCACCACCTCCAAGACGAGCCAAGTCCCCTGGTCTGGTTGGTCACTC 1785
Query 1781 AGCGCCTCTAACTGGATGGAACCAAGAAAGAAACACTGTCACTGTTGCTCCTCCGTCTGC 1840
|||||||||||| | ||||||||||||||||| |||||||||||||||| |||||| ||
Sbjct 1786 AGCGCCTCTAACCGCATGGAACCAAGAAAGAAGCACTGTCACTGTTGCTGTTCCGTCCGC 1845
Query 1841 CACCAACATTGTGGCTTCACCACTTCCGGTTCCTCCGTTGGTTGTCCCTAGAAGCTACTC 1900
||||||||||||||| || || ||||||||||||||||||||||||||||||||||||||
Sbjct 1846 CACCAACATTGTGGCCTCGCCGCTTCCGGTTCCTCCGTTGGTTGTCCCTAGAAGCTACTC 1905
Query 1901 TATACCTTCAAGAAACCAGAGAGCTGTTTCTCAACGGCCGGTCGAAAGGAGAGATGATAT 1960
||||||||||||||||||||||| |||||||||||||| |||||||||||||||||||||
Sbjct 1906 TATACCTTCAAGAAACCAGAGAGTTGTTTCTCAACGGCTGGTCGAAAGGAGAGATGATAT 1965
Query 1961 AGTAGCATCCCCACCGTTAACACCAATGAGCCTGTCTCGGCCTCTTCCTCAAGCCACAGG 2020
||||||||||||||||||||||||||||||||||||| |||| |||||||||||||||||
Sbjct 1966 AGTAGCATCCCCACCGTTAACACCAATGAGCCTGTCTAGGCCACTTCCTCAAGCCACAGG 2025
Query 2021 AGTTGCTCAGACCAGTCAAATCAGAGGAGTAGGAAAGCTGATCGAACGATGAGGCTGCCG 2080
|||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
Sbjct 2026 AGTTGCTCAGACCAGTCAAATCAGAGGAGTAGGAAAGCTGATCGAACGATGAGGCTTCCG 2085
Query 2081 CGTCTGCGTCGCGTTTGACCAAAACGCTTG-TTGTACAAATTCAGAGATCTTCACAAGTG 2139
|||||||||| ||||||||||||||||| | |||||||||||||||||||||||||||||
Sbjct 2086 CGTCTGCGTCACGTTTGACCAAAACGCT-GATTGTACAAATTCAGAGATCTTCACAAGTG 2144
Query 2140 AATATAGAAGTCGGTATATGTATTATTCTTTAGTTGGTTAATCTC-TTTTTTCTTTTCCC 2198
|||||||||||| |||||||||||||||||||||||||||||||| |||||| |||||||
Sbjct 2145 AATATAGAAGTCAGTATATGTATTATTCTTTAGTTGGTTAATCTCCTTTTTT-TTTTCCC 2203
Query 2199 ATTGTAAATCTGAAGTCTCTGCTCTTTATTCCATTTTCATTTTCAATCCC 2248
||||||||||||||| |||||||||||||||| |||||||||||||||||
Sbjct 2204 ATTGTAAATCTGAAGCCTCTGCTCTTTATTCCCTTTTCATTTTCAATCCC 2253
> AT5G41100.1 | Symbols: | FUNCTIONS IN: molecular_function unknown;
INVOLVED IN: biological_process unknown; LOCATED IN:
plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED
DURING: 13 growth stages; BEST Arabidopsis thaliana protein
match is: hydroxyproline-rich glycoprotein family protein
(TAIR:AT3G26910.2); Has 1503 Blast hits to 1197 proteins in
220 species: Archae - 4; Bacteria - 108; Metazoa - 481; Fungi
- 318; Plants - 186; Viruses - 39; Other Eukaryotes - 367
(source: NCBI BLink). | chr5:16447222-16450881 FORWARD LENGTH=2239
Length=2239
Score = 547 bits (296), Expect = 1e-154
Identities = 937/1233 (76%), Gaps = 98/1233 (8%)
Strand=Plus/Plus
Query 221 GATGAAAGCTTCGATTGAGAA-GTTAAGGAGATTAACATCGCATTCACATAAGGTTGATG 279
||||||||||||| ||| ||| |||||| ||| | ||||||| || || | ||||
Sbjct 210 GATGAAAGCTTCGTTTG-GAAGGTTAAGAAGA-T----TCGCATT-ACCCAAAGCCGATG 262
Query 280 TGAAGGAGAAAGGAGAGGTCATGGCTACAACACAAATTGACGAACTCGATCGAGCCGGGA 339
|| || | ||||||| | | | || |||||||||| | || | |||||||| |
Sbjct 263 CGATTGATATAGGAGAGCTTTTTCCCACTGCACAAATTGAAGGTCTTGCTCGAGCCGCCA 322
Query 340 AGGATATGCAAGATATGAGAGACTGCTACGATAGACTACTCTCCGCAGCTGCTGCCACGG 399
|||||||||||||||||||||| | || |||||| ||||| | ||||||||| | ||
Sbjct 323 AGGATATGCAAGATATGAGAGAGGGTTATGATAGATTACTCGAAGTAGCTGCTGCTATGG 382
Query 400 CTAATAGCGCTTATGAGTTCTCTGAGTCTTTGGGAGAAATGGGTTCTTGTTTGGAGCAAA 459
|||||||||| |||||||||||||| || ||||| |||||||| || || ||||||||||
Sbjct 383 CTAATAGCGCATATGAGTTCTCTGAATCATTGGGGGAAATGGGCTCATGCTTGGAGCAAA 442
Query 460 TCGCGCCTCATAACGA-CGAAGAAAGCAGTAGAA-TCTTGTTTATGTTGGGTAAAGTTCA 517
||||||||||||| || | ||||||| || ||| |||| | ||||| |||||||||||
Sbjct 443 TCGCGCCTCATAATGATC-AAGAAAGTGGTGGAATTCTT-CTAATGTTAGGTAAAGTTCA 500
Query 518 GAGTGAGCTTCAA-AGACTTC-TTGACACATATCGTAGTCATATATTCAAAACCATTACA 575
| ||||||| || | || || | ||||| |||||| ||| |||||||| |||||||||
Sbjct 501 GTTTGAGCTT-AAGAAAC-TCGTCGACACTTATCGTTCTCAAATATTCAAGACCATTACA 558
Query 576 TC-CCCATCAGAGGCGCTTCTCAAG-GACCTCAGAACTGTTGAGGATATGAAGCAACAAT 633
| || |||||| | |||||| || ||||| ||||||||||||||||||||||||||||
Sbjct 559 -CGACCTTCAGAGTCACTTCTC-AGTGACCTTAGAACTGTTGAGGATATGAAGCAACAAT 616
Query 634 GCGACGAGAAGAG-GAATGT-GT-ATG-AGATG-TC--G---C-TAGTAAAAGATAAAGG 682
| || || ||||| || || || | | | ||| | | | | || ||||| || ||
Sbjct 617 GTGAAGAAAAGAGAGAC-GTTGTTAAGCACATGCTAATGGAGCAT-GTGAAAGACAA-GG 673
Query 683 -A-AGGCTTAAAAGCAGTAAAGGAGAGAGGCATATTCCTCC--CGAGTCTCGAC-CTGCT 737
| | | ||||| ||| |||||| ||||| | ||||| || | || || || |||||
Sbjct 674 TACAAG-TTAAAGGCACTAAAGGGGAGAGACTTATTCG-CCGTC-AG-CTAGAGACTGCT 729
Query 738 TAC-AGTGAGTTTCATGACGAAGCAACAATGTGCATTTTTCGATTGAAATCTCTTAAAGA 796
| | |||| | || || || || || |||||||||| |||||||||||||| || ||
Sbjct 730 CGCGA-TGAGCTACAAGATGAGGCGACTCTGTGCATTTTCCGATTGAAATCTCTCAAGGA 788
Query 797 AGGACAAGCTCGTAGTCTCCTTACACAAGCAGTCCGTCACCACACTGCTCAGATGCGTTT 856
||| |||||||| |||||||| |||||||||| ||| ||||||||||||||||||| | |
Sbjct 789 AGGGCAAGCTCGAAGTCTCCTCACACAAGCAGCCCGCCACCACACTGCTCAGATGCATAT 848
Query 857 GTTCCACACTGGACTGAAATCTCTCGAGGCAGTTGAGCGTCATGTAA-AAGTTGCTGCAG 915
|||| |||| |||||||| || |||||||| |||| || || | || |||||||||
Sbjct 849 GTTCTTTGCTGGTCTGAAATCGCTTGAGGCAGTAGAGCAACACGTCAGAA-TTGCTGCAG 907
Query 916 AGAAACAACACATTGACTGTGAT-CTATCTGTTCATGGGAACGAGGTGGAA-GCTAGCGA 973
| | ||||||||| ||||||| | || |||| || |||||||| |||| | ||| ||
Sbjct 908 ATAGACAACACATCGACTGTG-TGCTCTCTGATCCCGGGAACGAAATGGATTG-TAGTGA 965
Query 974 GTatgatgatgatgatgatAGCCGA-TATATGAACAGAGAAGGAGAACTCAGTTTTGATT 1032
| || ||||||||||||| |||| | | | || ||||| ||||||||||||||||| |
Sbjct 966 GGATAATGATGATGATGA---CCGACT-TGTTAATAGAGATGGAGAACTCAGTTTTGACT 1021
Query 1033 ACAGAACAAATGACCAGA-ATGTAGAAGCTGCTTCTCTCTCTACACC--ATGGGGC--TC 1087
||| ||||| ||| |||| | ||||||| | | | ||| ||||| |||| | |
Sbjct 1022 ACATAACAAGTGAGCAGAGA-GTAGAAG-T--TA-TATCT--ACACCTCATGGATCGATG 1074
Query 1088 AAAGATGGATGATACAGACCTCTCGTTTCCTCGCCCTTCTACAA-CACGACCAGCAGCGG 1146
|| ||||||||| ||||| |||||||||| |||||||| || || || ||||| | |
Sbjct 1075 AA-GATGGATGACACAGATCTCTCGTTTCAACGCCCTTC-ACCTGCAGGATCAGCAACAG 1132
Query 1147 TAAATGCTGATCATAGAGAAGAATA-TCCAGTTTCAACCCGCGATA--AGTATTTGAGCA 1203
||||||| || | ||||||||| | || |||||||| ||| ||| || | |||||
Sbjct 1133 TAAATGCAGACCCTAGAGAAGAGCACTC-AGTTTCAAACCGTGATCGCAGAAC--GAGCA 1189
Query 1204 GCCATTCAGCTCCGT-TGTTCCCAGAAAAGAAACCTGATTTATCAGAGAGGTTGA-GACA 1261
|||||||||| || | |||| || || |||||| |||||||| |||| || | || ||
Sbjct 1190 GCCATTCAGCACC-TCTGTTTCCGGATAAGAAAGCTGATTTAGCAGATAGATCGATGA-- 1246
Query 1262 GGTGG-TGAATCCATCTTTTAATGCCTACGTATTACCAACACCAAACGATTCAAGGTACT 1320
|| | ||| ||||||| ||||| ||| |||||||||| || ||| |||| || ||
Sbjct 1247 GGCAGATGACTCCATCTGCAAATGCTTACATATTACCAACTCCCGTCGACTCAAAGTCCT 1306
Query 1321 CGAAACCGGT-TTCCCAAG-CTT-TAAATCCGAGGCCAGCAAACCACAGTGCTGGAAACA 1377
| | || | ||| ||| | | | | || || | ||||||||||||| ||||
Sbjct 1307 C-A--CCAATCTTCACAAAACCTGTCAC-CC-AG---A-CAAACCACAGTGC---AAACT 1354
Query 1378 TATGGCATTCATCTCCGTTAGAGCCGATAAAAA 1410
|||||||||||||||| |||| || |||||||
Sbjct 1355 TATGGCATTCATCTCCACTAGAACCAATAAAAA 1387
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 111545610990
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5