BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg485239
Length=2050
Score E
Sequences producing significant alignments: (Bits) Value
AT3G48860.2 | Symbols: | unknown protein; INVOLVED IN: biologi... 3086 0.0
AT5G23700.1 | Symbols: | unknown protein; BEST Arabidopsis tha... 802 0.0
> AT3G48860.2 | Symbols: | unknown protein; INVOLVED IN: biological_process
unknown; LOCATED IN: plasma membrane; EXPRESSED
IN: 22 plant structures; EXPRESSED DURING: 13 growth stages;
BEST Arabidopsis thaliana protein match is: unknown protein
(TAIR:AT5G23700.1); Has 12429 Blast hits to 9751 proteins
in 897 species: Archae - 180; Bacteria - 1190; Metazoa - 6552;
Fungi - 1361; Plants - 886; Viruses - 50; Other Eukaryotes
- 2210 (source: NCBI BLink). | chr3:18117486-18122093 FORWARD
LENGTH=2107
Length=2107
Score = 3086 bits (1671), Expect = 0.0
Identities = 1927/2047 (94%), Gaps = 31/2047 (2%)
Strand=Plus/Plus
Query 1 TTTTCCCTTCCGTTTTCGCGAGAAAATTTAGC-TTTTCCCCGAGAAAAATCCATTTTCTC 59
||||||||||||||||| ||||||||| || | ||||||||||| ||| | |||||||||
Sbjct 2 TTTTCCCTTCCGTTTTCTCGAGAAAATCTACCTTTTTCCCCGAG-AAATTTCATTTTCTC 60
Query 60 TGGTTCATCTTCTTTTTGCTCGCTTAAATACAGCGATTGCATCCAGCTAATTAGTAACTT 119
||||||| || ||| ||||||||||||||||||||||| |||||| | || |||| |||
Sbjct 61 TGGTTCACCTGCTTGTTGCTCGCTTAAATACAGCGATTTCATCCAACCAACAAGTACCTT 120
Query 120 CACGAACTCTCTGATGGATCGGAGGAGGGCGGGTAGCCCAGTGTATGGTCGCCAGTGGAG 179
||| ||| | ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 121 CACCAACACACTGATGGATCGGAGGAGGGCGGGTAGCCCAGTGTATGGTCGCCAGTGGAG 180
Query 180 CGAATCATCGAACAGTACGGAAACGACATCGCCGGCGATGTCTCCTGCTCACCGGAAGCA 239
||||||||||||||||||||| | ||||| |||||||||||||||||||||||||| ||
Sbjct 181 CGAATCATCGAACAGTACGGAGGCAACATCACCGGCGATGTCTCCTGCTCACCGGAAACA 240
Query 240 GCTCGGTGGTATCGGAGGTTTCTCCACCGTCAAACGGACTCAGAACGTAGCTGCAAAAGC 299
|||||||||||||||||||||||||||||| ||||||||||||||||||||||| |||||
Sbjct 241 GCTCGGTGGTATCGGAGGTTTCTCCACCGTTAAACGGACTCAGAACGTAGCTGCCAAAGC 300
Query 300 GGCGGCACAACGATTGGCTAAGGTTATGGCCTTGCAGAACAAAGACAACGGAGACGAAGA 359
|||||||| ||||| |||||||| |||||||| ||||||||||||||||||||||||||
Sbjct 301 CGCGGCACAGCGATTAGCTAAGGTCATGGCCTTACAGAACAAAGACAACGGAGACGAAGA 360
Query 360 TGATGAAGATGAAGATGAAGATCTAAGTCTCAGATTCGCATCTCCTTCTTTGAAACCTGC 419
||||||||||||||||||||||||||||||||||||||||||| ||||||| ||||||||
Sbjct 361 TGATGAAGATGAAGATGAAGATCTAAGTCTCAGATTCGCATCTGCTTCTTTAAAACCTGC 420
Query 420 TCGTCATGCACCTTCTTCTCTTTCCTCTACTGGAAGTAATAGCAGCAACGGAAACAGTAA 479
||||||||||||||||||| | ||||| || ||||| ||||||||||||||||| | |||
Sbjct 421 TCGTCATGCACCTTCTTCTATCTCCTCCACCGGAAGCAATAGCAGCAACGGAAATAATAA 480
Query 480 TTTGCCGGCTGTTTC-TTTCGCTCCCCGAACTAGATCGCCGTCTCCTGCGTTAGGCAGGA 538
||||||||||||||| ||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 481 TTTGCCGGCTGTTTCATTT-GCTCCCCGAACTAGATCGCCGTCTCCTGCGTTAGGCAGGA 539
Query 539 ACTTCGCCGAGCAAGT-GC--CTTCAGTGCGTTCTGCTTCAGCCGGAAGGCCGTCAATGT 595
|||||||||||||||| | |||||||||| ||||||||||||||||||||||||||||
Sbjct 540 ACTTCGCCGAGCAAGTACCTTCTTCAGTGCGGTCTGCTTCAGCCGGAAGGCCGTCAATGT 599
Query 596 CTGCTAGATCCACGACTCCGACTCCGATTCCTAATCTAATGCCGCCTAGTAGGGTGTCGG 655
|||||||||| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 600 CTGCTAGATCTACGACTCCGACTCCGATTCCTAATCTAATGCCGCCTAGTAGGGTGTCGG 659
Query 656 TGAAAACTCCGGTTTCTATTCCTCCACTTGATCCTCCAACTAGGAGCAGAGATAAAAGGT 715
||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
Sbjct 660 TGAAAACTCCGGTTTCTATTCCTCCACTTGATCCTCCAACTCGGAGCAGAGATAAAAGGT 719
Query 716 TTTTTGCTGATGTACCATCAGTAAATTCAAAAGAAAAGGGTGATCAGCGGGAAGCTTCAG 775
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 720 TCTTTGCTGATGTACCATCAGTAAATTCAAAAGAAAAGGGTGATCAGCGGGAAGCTTCAG 779
Query 776 CTCTCCGTGATGAACTGGATATGCTACAAGAGGAGAATGAGAATGTACTGGAGAAGCTTC 835
|||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 780 CTCTTCGTGATGAACTGGATATGCTACAAGAGGAGAATGAGAATGTACTGGAGAAGCTTC 839
Query 836 GTCGTGCGGAAGAAAAAAGGGTGGAAGCAGAAGCTAGGGCTAAGGAGCTCGAAAAACAGG 895
|||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
Sbjct 840 GTCGTGCGGAAGAAAAAAGGGTGGAAGCAGAAGCTAGGGCTAAGGAGCTCGAGAAACAGG 899
Query 896 TTGCCTCTCTTGGAGAAGGAGTGTCCCTGGAGGCGAAATTGTTAAGCAGGAAAGAAGCAG 955
|||||||||||||||||||||| ||| |||||||||||||||||||||||||||||||||
Sbjct 900 TTGCCTCTCTTGGAGAAGGAGTTTCCTTGGAGGCGAAATTGTTAAGCAGGAAAGAAGCAG 959
Query 956 CGTTGCGTCAAAGAGAGGCTGCTCTAAATGCTGCTAAACAGAAAAAGAGTGGAAAGGATG 1015
| |||||||||||||||||||||||||||| ||||||||| |||||||||||||||||||
Sbjct 960 CATTGCGTCAAAGAGAGGCTGCTCTAAATGTTGCTAAACAAAAAAAGAGTGGAAAGGATG 1019
Query 1016 ATGAAATTGTTTCCCTACGTTCGGAACTTGAGAACTTGAAAGACGAGGCTACAACTGCTG 1075
| ||||||||||||||||||||||| ||||||||||||||||| ||||||||||||||||
Sbjct 1020 AGGAAATTGTTTCCCTACGTTCGGAGCTTGAGAACTTGAAAGATGAGGCTACAACTGCTG 1079
Query 1076 CAGAACGGCTTCAAGAAGCAGAGTCTGAAGCAAAGTCTCTTCGCACAATGACTCAAAGGA 1135
||||||||||||||||||||||||||||||| ||||||||||||||||||||||| ||||
Sbjct 1080 CAGAACGGCTTCAAGAAGCAGAGTCTGAAGCGAAGTCTCTTCGCACAATGACTCAGAGGA 1139
Query 1136 TGATTTTAACACAGGACGAAATGGAAGAAGTAGTTCTGAAGAGATGCTGGCTTGCCCGGT 1195
|||||||||||||||| |||||||||||||||||||||||||||||||||||||| ||||
Sbjct 1140 TGATTTTAACACAGGATGAAATGGAAGAAGTAGTTCTGAAGAGATGCTGGCTTGCTCGGT 1199
Query 1196 ATTGGGGCTTGGCTGTGCAACATGGCATTTGTGCGGATATAGCACCATCCAGGCATGAGC 1255
||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
Sbjct 1200 ATTGGGGCTTGGCTGTGCAACATGGCATTTGTGCGGATATAGCACCATCCAGGCAAGAGC 1259
Query 1256 ACTGGTCAGCGTTGGCTCCTCTTCCATTTGAGCTCGTCACCTCTGCAGCACAGAAGGCGA 1315
|||||||| |||||||||||||||||||||||||||||| || ||||||||||||||||
Sbjct 1260 ACTGGTCAAAGTTGGCTCCTCTTCCATTTGAGCTCGTCACTTCAGCAGCACAGAAGGCGA 1319
Query 1316 AGGAGTTA-------A-AA-GGTGGGAATGATCGGAGTAAGGCCGCTAGAGATTTGAGTG 1366
|||||||| | || ||||||||||||||||||||||| ||||||||||||||||
Sbjct 1320 AGGAGTTATCTTGGGATAAAGGTGGGAATGATCGGAGTAAGGCGGCTAGAGATTTGAGTG 1379
Query 1367 ATCTAACTGGAGAAGGAAATATTGAAAGTATGCTTTCAGTTGAGATGGGTTTGAGAGAGC 1426
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1380 ATCTAACTGGAGAAGGAAATATTGAAAGTATGCTTTCAGTTGAGATGGGTTTGAGAGAGC 1439
Query 1427 TAGCATCATTAAAGGTTGAGGATGCTGTTGTGCTTGTATTCGCCCAGCAGAGAAAACTGA 1486
|||||||||||||||| |||||||||||||||||| ||||||||||||||||||| |||
Sbjct 1440 TAGCATCATTAAAGGTGGAGGATGCTGTTGTGCTTATATTCGCCCAGCAGAGAAAGTTGA 1499
Query 1487 GTTTGGTTCGTCAAACTGTTTCTGACTCCAAAGGTCATGGAGAATCCAGATTTATCGATG 1546
|||||||||| || |||||||| |||||||||||||||||||||||||||||||||||||
Sbjct 1500 GTTTGGTTCGCCACACTGTTTCCGACTCCAAAGGTCATGGAGAATCCAGATTTATCGATG 1559
Query 1547 CCTATGAACTGGGTGAAGCGGAACAAGAAGATGTTGCCTTTAAGCAGGCATGGCTGATGT 1606
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1560 CATATGAACTGGGTGAAGCGGAACAAGAAGATGTTGCCTTTAAGCAGGCATGGCTGATGT 1619
Query 1607 ACTTTTGGGGAAGAGCTAAGTTGCACGGTGTGGAAGATGACATCGCAGAGGAACGTGTCC 1666
|||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1620 ACTTCTGGGGAAGAGCTAAGTTGCACGGTGTGGAAGATGACATCGCAGAGGAACGTGTCC 1679
Query 1667 AGTTATGGATCAGCCGTAGCAGTGGGAAATCTCAGACGACTTCTCATGATGCCTTGGATG 1726
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1680 AGTTATGGATCAGCCGTAGCAGTGGGAAATCTCAGACGACTTCTCATGATGCCTTGGATG 1739
Query 1727 TGGAAAGAGGGTTGATAGAGCTAAGGAAACTGGGAATAGAGCAACAGTTGTGGGAAGCTT 1786
||||||||||||||||||||||| | ||||||||||||||||||||||||||||||||||
Sbjct 1740 TGGAAAGAGGGTTGATAGAGCTACGAAAACTGGGAATAGAGCAACAGTTGTGGGAAGCTT 1799
Query 1787 CTCGAAGAGAAATCGATCAGCTTCCATCGCCTACTTCTTCGAATCACGATCTCGATGCTG 1846
||||| ||||||||||||||||||| ||||||||||||||||||||||||||||||||||
Sbjct 1800 CTCGACGAGAAATCGATCAGCTTCCCTCGCCTACTTCTTCGAATCACGATCTCGATGCTG 1859
Query 1847 ATTCATAGTAAAGTCTCTTCCTCT-CTTTGGGAGAAGAAATTTTCGTCCTGTATAT-T-T 1903
|||||||||||||||||||||| | ||||| ||||||||||||||| ||||||| | |
Sbjct 1860 ATTCATAGTAAAGTCTCTTCCTTTTCTTTGATAGAAGAAATTTTCGTTTTGTATATCTCT 1919
Query 1904 CGTTTCATGTAAGTTTCACGAAGAATCTGT-GTGCCATTGTATTTTTCTTTCCCTTTTAG 1962
||| ||||||||||||||||||||||||| ||| |||||||||||| | | ||||
Sbjct 1920 CGT--CATGTAAGTTTCACGAAGAATCTGTTGTGTCATTGTATTTTT-TATG--TTTTCC 1974
Query 1963 -TTTTCACACTTGAGTGTGTAAGCA---G-TATTCTTATTTTTGCAATCAAGTCCAAAAA 2017
||||||||||| ||||||||||| | ||||||||||||||||||||| ||||||||
Sbjct 1975 CTTTTCACACTTAAGTGTGTAAGCTTTTGGTATTCTTATTTTTGCAATCAATTCCAAAAA 2034
Query 2018 -TTTTGG 2023
||||||
Sbjct 2035 GTTTTGG 2041
> AT5G23700.1 | Symbols: | unknown protein; BEST Arabidopsis thaliana
protein match is: unknown protein (TAIR:AT3G48860.2);
Has 30201 Blast hits to 17322 proteins in 780 species: Archae
- 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants
- 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI
BLink). | chr5:7992788-7996420 FORWARD LENGTH=1785
Length=1785
Score = 802 bits (434), Expect = 0.0
Identities = 841/1030 (82%), Gaps = 57/1030 (6%)
Strand=Plus/Plus
Query 492 TTCTTTCGCTCCCCG-AACTAGATCGCCGTCTCCTGCGTTAGGCAGGAACTTCGCCGAGC 550
||| ||||||| ||| || | |||||| || |||||| |||| |||||| || | ||||
Sbjct 348 TTCCTTCGCTCGCCGTAA-TCGATCGCATTCACCTGCGATAGGTAGGAACATCACTGAGC 406
Query 551 AAGTGCCTTCAGTGCGTTCTG-CTTCAG-CCGGAAGGCCGTCAATGTCT-GCTAGATCCA 607
||||| ||||||||||||| | | || | | |||||||| |||| || | | ||||| ||
Sbjct 407 AAGTGACTTCAGTGCGTTC-GTCGTC-GACAGGAAGGCCATCAACGTTTAG-TAGAT-CA 462
Query 608 -CGACTCCGACTCCGAT-TCCTAATCT-AATGCCGCCTAGTAGGGTGTCGGTGAAAACTC 664
| ||||||| | || | ||| | | ||||| |||| | || ||| ||||| |||
Sbjct 463 TCAACTCCGAATGCG-TCTCC-CCTTTGGATGCCACCTA--A-GGCATCGTTGAAACCTC 517
Query 665 CGGT-TTCTATTCCTCCACTTGATCCTCCAACT-AGGAGCAGAGATAAAAGGTTTTTTGC 722
|||| | |||||||||| |||||| | || | |||| |||||| |||| | ||||
Sbjct 518 CGGTGAT-TATTCCTCCAATTGATCATTCATTTAAGGA-TAGAGATCAAAGATACTTTGG 575
Query 723 TGATGTACCA---TCAGTAAATTCAAAAGA-AAAGGGTGATCAGCGGGAAGCTTCAGCTC 778
|||||||||| | ||||||||| | ||| || |||| ||||||| |||||||||||||
Sbjct 576 TGATGTACCACGCTTAGTAAATTCGAGAGATAAGGGGT-ATCAGCGAGAAGCTTCAGCTC 634
Query 779 TCCGTGATGAACTGGATATGCTACAAGAGGAGAATGAGAATGTACTGGAGAAGCTTCGTC 838
| ||||||||| ||||||||||||||||||||||||||| |||| |||||||||||| |
Sbjct 635 TTCGTGATGAAGTGGATATGCTACAAGAGGAGAATGAGATTGTATTGGAGAAGCTTCACC 694
Query 839 GTGCGGAAGAAAAAAGGGTGGAAGCAGAAGCTAGGGCTAAG-GAGCTCGAAAAACAGGTT 897
|||| ||||||| | | || ||||||||| |||||| | ||||| || |||||||||
Sbjct 695 GTGCAGAAGAAATGCGTGAGGCAGCAGAAGCCAGGGCT-CGAGAGCTGGAGAAACAGGTT 753
Query 898 GCCTCTCTTGGAGAAGGAGTGTCCCTGGAGGCGAAATTGTTAAGCAGGAAA-GAAGCAGC 956
|| |||||||||||||||||||| |||||||| ||||||||||| | |||| ||||| ||
Sbjct 754 GCTTCTCTTGGAGAAGGAGTGTCTCTGGAGGCTAAATTGTTAAGTA-GAAAGGAAGCGGC 812
Query 957 GTTGCGTCAAAGAGAGGCTGCTCTAAATGCTGCTAAAC-AG-AAAAAGAGT-GGAAAGGA 1013
||||| ||| | ||||||||||| || |||||| ||| || ||||||| | ||||| |
Sbjct 813 ATTGCGCCAACGTGAGGCTGCTCTTAAGGCTGCT-AACGAGAAAAAAGA-TGGGAAA-AA 869
Query 1014 TGATGAAATTGTTTCCCTACGTTCGGAACTTGAGAACTTGAAAGACGAGGCTA-CAACTG 1072
|| ||| |||||||||| ||||| ||| || ||| |||||| || ||||| | ||| |
Sbjct 870 GGAGGAAGTTGTTTCCCTCCGTTCAGAAATTCAGATCTTGAAGGATGAGGC-AGAAACAG 928
Query 1073 CTGCAGAACGGCTTCAAGAAGCAGAGTCTGAAGCAAAGTCTCTTCGCACAATGACTCAAA 1132
|||| ||| | ||||||||||| || || ||||||||| ||||||| | |||||||||||
Sbjct 929 CTGCGGAATGCCTTCAAGAAGCGGAATCAGAAGCAAAGGCTCTTCGTATAATGACTCAAA 988
Query 1133 GGATGATTTTAACACAGGACGAAATGGAAGAAGTAGTTC-TGAAGAGATGCTGGCTTGCC 1191
| ||| |||||||||||||||||||||| ||||||| | ||||||||||||||||||||
Sbjct 989 GAATGGTTTTAACACAGGACGAAATGGAGGAAGTAG-CCTTGAAGAGATGCTGGCTTGCC 1047
Query 1192 CGGTATTGGGGCTTGGCTGTGCAACATGGCATTTGTGCGGATATAGCACCATCCAGGCAT 1251
||||||||||| ||||| ||||| |||||||||||||| || ||||||||||||||||||
Sbjct 1048 CGGTATTGGGGTTTGGCAGTGCAGCATGGCATTTGTGCTGACATAGCACCATCCAGGCAT 1107
Query 1252 GAGCACTGGTCAGCGT-TGGCTCCTCTTCCATTTGAGCTCGTCACCTCTGCAGCACAGAA 1310
|| | ||||| || | |||||||||||||||||||||| |||| ||||||||||| ||
Sbjct 1108 GAAAAATGGTCGGC-TCTGGCTCCTCTTCCATTTGAGCTGGTCATATCTGCAGCACA-AA 1165
Query 1311 GGCGAAGGAGTTAAAAGGTGGGAATGATCGGAGTAAGGCCGCTAGAGATTTGAGTGATCT 1370
| | | || || | ||||| ||| || | |||||| ||| ||||||||
Sbjct 1166 ----A-G-ACC-AA--GG----A-TGATCAGAGCAAAACTGCTAGATTTTTAAGTGATCT 1211
Query 1371 AACTGGAGAAGGAAATATTGAAAGTATGCTTTCAGTTGAGATGGGTTTGAGAGAGCTAGC 1430
| |||||||||||||||| |||||||||||||||||||||||||||||||||||||| ||
Sbjct 1212 ACCTGGAGAAGGAAATATCGAAAGTATGCTTTCAGTTGAGATGGGTTTGAGAGAGCTGGC 1271
Query 1431 ATCATTAAAGGTTGAGGATGCTGTTGTGCTTGTATTCGCCCAGCAGAGAAAACTGAGTTT 1490
||| ||||||||||||||||||||| |||||| |||||| ||| |||||| || ||||||
Sbjct 1272 ATCCTTAAAGGTTGAGGATGCTGTTATGCTTGCATTCGCACAGAAGAGAACACCGAGTTT 1331
Query 1491 GGTTCGTCAA 1500
||||||||||
Sbjct 1332 GGTTCGTCAA 1341
Score = 243 bits (131), Expect = 7e-63
Identities = 215/254 (85%), Gaps = 11/254 (4%)
Strand=Plus/Plus
Query 1561 GAAGCGGAACAAGAAGATGTTGCCTTTAAGCAGGCATGGCTGATGTACTTTTGGGGAAGA 1620
|||| |||||||||||||||||||||||||||||||||||| |||||||| ||||| |||
Sbjct 1486 GAAGGGGAACAAGAAGATGTTGCCTTTAAGCAGGCATGGCTTATGTACTTCTGGGGTAGA 1545
Query 1621 GCTAAGTTGCACGGTGTGGAAGATGACATCGCAGAGGAACGTGTCCAGTTATGGATCAGC 1680
|| |||||||| |||||||||| ||||| ||||| |||||| ||||||| |||| |||
Sbjct 1546 GCCAAGTTGCATAGTGTGGAAGAAGACATTGCAGATGAACGTTTCCAGTTTTGGACCAGT 1605
Query 1681 CGTAGCAGTGGGAAA-TCTCAGACGACTTCTCATGATGCC-TTGGATGTGGAAAGAGGGT 1738
||||| | ||||| |||| ||||||| || |||||| ||| | |||||| |||| |
Sbjct 1606 CGTAGT-GAAGGAAAATCTC---CGACTTCACAAGATGCCGTTG-ACGTGGAACGAGGTT 1660
Query 1739 TGATAGAGCTAAGGAAACTGGGAATAGAGCAACAGTTGTGGGAAGCTTCTCG-AAGAGAA 1797
|| |||||||||||||||| ||| |||||||||| || |||||||||| ||| || ||||
Sbjct 1661 TGTTAGAGCTAAGGAAACTAGGAGTAGAGCAACAATTATGGGAAGCTTGTCGCAA-AGAA 1719
Query 1798 ATC-GATCAGCTTC 1810
| | ||||||||||
Sbjct 1720 A-CAGATCAGCTTC 1732
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 101605903080
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5