BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg485867
Length=1820
Score E
Sequences producing significant alignments: (Bits) Value
AT3G54630.1 | Symbols: | CONTAINS InterPro DOMAIN/s: Kinetocho... 2623 0.0
AT3G54625.1 | Symbols: | other RNA | chr3:20220810-20223024 FO... 2484 0.0
> AT3G54630.1 | Symbols: | CONTAINS InterPro DOMAIN/s: Kinetochore
protein Ndc80 (InterPro:IPR005550); Has 24780 Blast hits
to 15608 proteins in 1321 species: Archae - 545; Bacteria
- 2969; Metazoa - 12597; Fungi - 2181; Plants - 1581; Viruses
- 39; Other Eukaryotes - 4868 (source: NCBI BLink). | chr3:20221097-20223028
REVERSE LENGTH=1932
Length=1932
Score = 2623 bits (1420), Expect = 0.0
Identities = 1667/1786 (93%), Gaps = 17/1786 (1%)
Strand=Plus/Plus
Query 45 TTATCTGTaaaaaagc-aaaaaGAGATGAGAGGAGGAGCCACGGGAAAGAGAAGAACGAC 103
|||||||| ||||||| |||||||||||||||| |||||| |||||||||||||||||||
Sbjct 104 TTATCTGT-AAAAAGCAAAAAAGAGATGAGAGGCGGAGCCGCGGGAAAGAGAAGAACGAC 162
Query 104 GGTGGGTTTCGGCGGAGCGCCGCCGCCACCACCACCATCAATCGAGCAACACCGTCACCT 163
|||||||||||||||||||||||||||||| |||||||||||||||||||| ||||||||
Sbjct 163 GGTGGGTTTCGGCGGAGCGCCGCCGCCACCTCCACCATCAATCGAGCAACAACGTCACCT 222
Query 164 CTTTAACTCAAGAGACTCCGACGCCAGTTTCGCCAGCAGCCGTCCTTCATCAATCGGTTT 223
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 223 CTTTAACTCAAGAGACTCCGACGCCAGTTTCGCCAGCAGCCGTCCTTCATCAATCGGTTT 282
Query 224 AGGCGGCC---GAGCCTCCGATGACAGATCTTCGATGATTCGATTCACCAACGCCTTTCT 280
|||||||| |||||||||||||||||||||||||||||||||||| |||||||| ||
Sbjct 283 AGGCGGCCGAGGAGCCTCCGATGACAGATCTTCGATGATTCGATTCATTAACGCCTTCCT 342
Query 281 CTCCTCTCATAAC-TCTCTGATCTCTATCCGCGCTAATCCTGTTCCTTCTGTCAAAGACA 339
|||| | |||||| || | |||||||||||||| ||||||||||||||||||||||||||
Sbjct 343 CTCCACACATAACTTC-CCGATCTCTATCCGCGGTAATCCTGTTCCTTCTGTCAAAGACA 401
Query 340 TCTCCGAAACCCTAAAATTCATT-CTCTCCA-AATTAGATTACCCTTGCGATTCCATCAA 397
|||||||||||||||||||| || |||| || ||||||||||||||||||||||||||
Sbjct 402 TCTCCGAAACCCTAAAATTC-TTGCTCT-CAGCTTTAGATTACCCTTGCGATTCCATCAA 459
Query 398 ATGGGACGAGGATCTCGTCTTCTTCCTCAAATCGCAGAAATGTCCATTCAAAATCACTAA 457
|||||||||||||||||||||||| |||||||||||||||||||| || || ||||| ||
Sbjct 460 ATGGGACGAGGATCTCGTCTTCTTTCTCAAATCGCAGAAATGTCCTTTTAAGATCACCAA 519
Query 458 ATCTAGCCTCAAAGCTCCCAATACGCCTCACAATTGGCCTACTGTTCTCGCCGTTGTTCA 517
|||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 520 ATCTAGCCTCAAAGCTCCAAATACGCCTCACAATTGGCCTACTGTTCTCGCCGTTGTTCA 579
Query 518 CTGGTTGGTTGAGCTCGCTCGTTTCCATCAACATCTA-TCATCCAATTCCACCTCTGAAC 576
|||||| | |||||| ||||||||||||||||||||| | ||| ||||||||||| | ||
Sbjct 580 CTGGTTAGCTGAGCTTGCTCGTTTCCATCAACATCTAGT-ATCTAATTCCACCTCCGTAC 638
Query 577 CCGAAGCTAACTCCATGAATTTCTTCGCGATTCAAAGCTTTAGTCACTTCATTCGCGGCG 636
|||||| |||||||||||||||||||||||||||||||||| ||||||||||||||||||
Sbjct 639 CCGAAGATAACTCCATGAATTTCTTCGCGATTCAAAGCTTTGGTCACTTCATTCGCGGCG 698
Query 637 AGGATGATAAAGTTAACGAGTTAGATTCTGAATTTTTGGGTAAATTGGAAGCGGAAAAGA 696
|||||||||| |||||||| ||||||||| ||||||||||||||||||||||||| ||||
Sbjct 699 AGGATGATAAGGTTAACGATTTAGATTCTCAATTTTTGGGTAAATTGGAAGCGGAGAAGA 758
Query 697 CTAGCGTCGCTGAAACTATCTCTGGTTGTGAGAAAATCTCCGGTGAATTGGAGGCTAAAC 756
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 759 CTAGCGTCGCTGAAACTATCTCTGGTTGTGAGAAAATCTCCGGTGAATTGGAGGCTAAAC 818
Query 757 TTGAGTCACTGAGGAAAGGTCCGTCGAAGAAAGAGTCTCTGGGGAAAGCTAAAGCCGATT 816
|||||||||||||||||||||| ||||||||||||||||||| ||||| |||||||||||
Sbjct 819 TTGAGTCACTGAGGAAAGGTCCTTCGAAGAAAGAGTCTCTGGAGAAAGTTAAAGCCGATT 878
Query 817 TGGAGAATGACGTGAACAAGTTCCGAACTATTGTTGCGGAATATACTGAAAAGACTCAAG 876
||||||||||||| |||||||||||||||||||||| ||| |||||||| | || || ||
Sbjct 879 TGGAGAATGACGTAAACAAGTTCCGAACTATTGTTGTGGAGTATACTGATAGGAATCCAG 938
Query 877 CGCTGGAGAAAGTTGTGGAGGAGAAAGTTAAGGAGCTTAAAGCTAAGgaagaagagagag 936
|| |||||||||||||||||||||||| ||||||||| ||||||||||||||||||||||
Sbjct 939 CGATGGAGAAAGTTGTGGAGGAGAAAGCTAAGGAGCTCAAAGCTAAGGAAGAAGAGAGAG 998
Query 937 agcgaatctctgaagagaataaagagttgaagaagagTGTGGACCTTCAGAACTTTAGCG 996
|||||||||||| ||||||||| |||||||||||||||||||| ||||||||||||||||
Sbjct 999 AGCGAATCTCTGTAGAGAATAAGGAGTTGAAGAAGAGTGTGGAGCTTCAGAACTTTAGCG 1058
Query 997 CCAGAG-ATGTAGAGAGGATGAGAAGAGAGTTACAAGCTGTGGAAAGAGATGTTGCAGAG 1055
| | | ||||| | ||||||||||||||||||||||||||||||||||||||||| ||
Sbjct 1059 CG-GCGGATGTAAATAGGATGAGAAGAGAGTTACAAGCTGTGGAAAGAGATGTTGCGGAT 1117
Query 1056 GCTGAGGTTGCTAGAGATGGTTGGGATCTGAAAGCTTGGGAACTCAATTCACAGATTGGG 1115
|||||||||||||||||||||||||||| |||||||||||||||||||||||||||| ||
Sbjct 1118 GCTGAGGTTGCTAGAGATGGTTGGGATCAGAAAGCTTGGGAACTCAATTCACAGATTAGG 1177
Query 1116 AATCAGTTTCATCAGATTCAGACACTTTCCATTGATTGTAACCAAGTTCTTAGGAGATTG 1175
||||||||||||||||||||||||||| ||||||| || ||||||| ||| |||||||||
Sbjct 1178 AATCAGTTTCATCAGATTCAGACACTTGCCATTGACTGCAACCAAGCTCTCAGGAGATTG 1237
Query 1176 AAGCTTGATATTCAGTTCACTGTAAATGAGAGAGGGAAAACACCTGCAAAGGTGATTGGT 1235
|||||||||||||||||| |||| || ||||||||| ||||||||||| |||||| |||
Sbjct 1238 AAGCTTGATATTCAGTTCGCTGTTAACGAGAGAGGGGAAACACCTGCAGCGGTGATGGGT 1297
Query 1236 GTGGATTATAAATCTGTGGTGAAGCCAGCTCTGTGTTCGCTGTACGATGGGATTAAAGGA 1295
|||||||||||||| |||||||||||||||||||||||| ||| ||||||||| ||||||
Sbjct 1298 GTGGATTATAAATCGGTGGTGAAGCCAGCTCTGTGTTCGTTGTGCGATGGGATCAAAGGA 1357
Query 1296 AGCTTGACGGAGAAAATGGAGGAGTTGGTCACTTTACAGCAAGAGGTGTCTGAAATGGCT 1355
|||| | |||||||| ||||||| ||||||||||||||||| | |||||||||||||
Sbjct 1358 AGCTCGGCGGAGAAAGTGGAGGAATTGGTCACTTTACAGCACCATAAGTCTGAAATGGCT 1417
Query 1356 TCGAAGATCGAATCCAAAAAGAGTCTTCTTGGGTCAATTCAGTTGCAGATCAATGAACTC 1415
||||||||||||||||||| |||||||||||||||||||||||||||||||||||| |||
Sbjct 1418 TCGAAGATCGAATCCAAAAGGAGTCTTCTTGGGTCAATTCAGTTGCAGATCAATGACCTC 1477
Query 1416 GAAGAAAAGATGAAGTTGGTGAAGAAGGAAACTCAGGAGTTGACTACAAAATGTGACTTG 1475
||||||||||||||||||||||||||||||||||||||||||| |||||||||||| |||
Sbjct 1478 GAAGAAAAGATGAAGTTGGTGAAGAAGGAAACTCAGGAGTTGAGTACAAAATGTGATTTG 1537
Query 1476 GAAGCGAAAACATTGGTTGAGAGTGTGAAAACGGAGGCTTTGAATCTGGAGGTTGTGGAG 1535
|| ||||||||||||||||||||||||||| | |||||||||||||||||||||||||||
Sbjct 1538 GAGGCGAAAACATTGGTTGAGAGTGTGAAAGCTGAGGCTTTGAATCTGGAGGTTGTGGAG 1597
Query 1536 AAAGAAGCTGCTGAGTTTTTAAAAGCTTCGGAGGTAAGATTGCAAGAAGCAGAAAAGCAG 1595
|||||||||||||||||| |||||||||||||| | |||||||||||||||| ||| ||
Sbjct 1598 AAAGAAGCTGCTGAGTTTGTAAAAGCTTCGGAGCTTAGATTGCAAGAAGCAGTGAAGGAG 1657
Query 1596 AGTGAAGAGGAGGTCCAAGCATGTGCTGCACAACTATTTGCACTTATCGATTCGATATCG 1655
||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
Sbjct 1658 AGTGAAGAGGAGGTCCAAGCATGTGCTGCACAACTCTTTGCACTTATCGATTCGATATCG 1717
Query 1656 AAACAGAAAGAGTACATGGATTTGAAGATATTGGAGATCAAAACTGGTGTTGCTGATACT 1715
|||||||||||||||||||||| |||||||| |||||||||||| |||||||||||||||
Sbjct 1718 AAACAGAAAGAGTACATGGATTCGAAGATATCGGAGATCAAAACCGGTGTTGCTGATACT 1777
Query 1716 GCCAGTGCTGTTTCAGAGATATATAAAGCCAACTTCAAGAAGCACCTAGGTATCTAG-GG 1774
||||||||||||||||||||||| |||||||||||||||||| |||| ||||||||| ||
Sbjct 1778 GCCAGTGCTGTTTCAGAGATATACAAAGCCAACTTCAAGAAGAACCTTGGTATCTAGTGG 1837
Query 1775 ATTGGCATCAACTGAGCCCAGAGATTGTTAAAAGCGTGAGTAATTC 1820
|||| |||||||||||| |||||||||||||||||||||||||||
Sbjct 1838 -TTGGAATCAACTGAGCCAAGAGATTGTTAAAAGCGTGAGTAATTC 1882
> AT3G54625.1 | Symbols: | other RNA | chr3:20220810-20223024
FORWARD LENGTH=1890
Length=1890
Score = 2484 bits (1345), Expect = 0.0
Identities = 1577/1689 (93%), Gaps = 15/1689 (1%)
Strand=Plus/Minus
Query 45 TTATCTGTaaaaaagc-aaaaaGAGATGAGAGGAGGAGCCACGGGAAAGAGAAGAACGAC 103
|||||||| ||||||| |||||||||||||||| |||||| |||||||||||||||||||
Sbjct 1791 TTATCTGT-AAAAAGCAAAAAAGAGATGAGAGGCGGAGCCGCGGGAAAGAGAAGAACGAC 1733
Query 104 GGTGGGTTTCGGCGGAGCGCCGCCGCCACCACCACCATCAATCGAGCAACACCGTCACCT 163
|||||||||||||||||||||||||||||| |||||||||||||||||||| ||||||||
Sbjct 1732 GGTGGGTTTCGGCGGAGCGCCGCCGCCACCTCCACCATCAATCGAGCAACAACGTCACCT 1673
Query 164 CTTTAACTCAAGAGACTCCGACGCCAGTTTCGCCAGCAGCCGTCCTTCATCAATCGGTTT 223
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1672 CTTTAACTCAAGAGACTCCGACGCCAGTTTCGCCAGCAGCCGTCCTTCATCAATCGGTTT 1613
Query 224 AGGCGGCC---GAGCCTCCGATGACAGATCTTCGATGATTCGATTCACCAACGCCTTTCT 280
|||||||| |||||||||||||||||||||||||||||||||||| |||||||| ||
Sbjct 1612 AGGCGGCCGAGGAGCCTCCGATGACAGATCTTCGATGATTCGATTCATTAACGCCTTCCT 1553
Query 281 CTCCTCTCATAAC-TCTCTGATCTCTATCCGCGCTAATCCTGTTCCTTCTGTCAAAGACA 339
|||| | |||||| || | |||||||||||||| ||||||||||||||||||||||||||
Sbjct 1552 CTCCACACATAACTTC-CCGATCTCTATCCGCGGTAATCCTGTTCCTTCTGTCAAAGACA 1494
Query 340 TCTCCGAAACCCTAAAATTCATT-CTCTCCA-AATTAGATTACCCTTGCGATTCCATCAA 397
|||||||||||||||||||| || |||| || ||||||||||||||||||||||||||
Sbjct 1493 TCTCCGAAACCCTAAAATTC-TTGCTCT-CAGCTTTAGATTACCCTTGCGATTCCATCAA 1436
Query 398 ATGGGACGAGGATCTCGTCTTCTTCCTCAAATCGCAGAAATGTCCATTCAAAATCACTAA 457
|||||||||||||||||||||||| |||||||||||||||||||| || || ||||| ||
Sbjct 1435 ATGGGACGAGGATCTCGTCTTCTTTCTCAAATCGCAGAAATGTCCTTTTAAGATCACCAA 1376
Query 458 ATCTAGCCTCAAAGCTCCCAATACGCCTCACAATTGGCCTACTGTTCTCGCCGTTGTTCA 517
|||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 1375 ATCTAGCCTCAAAGCTCCAAATACGCCTCACAATTGGCCTACTGTTCTCGCCGTTGTTCA 1316
Query 518 CTGGTTGGTTGAGCTCGCTCGTTTCCATCAACATCTA-TCATCCAATTCCACCTCTGAAC 576
|||||| | |||||| ||||||||||||||||||||| | ||| ||||||||||| | ||
Sbjct 1315 CTGGTTAGCTGAGCTTGCTCGTTTCCATCAACATCTAGT-ATCTAATTCCACCTCCGTAC 1257
Query 577 CCGAAGCTAACTCCATGAATTTCTTCGCGATTCAAAGCTTTAGTCACTTCATTCGCGGCG 636
|||||| |||||||||||||||||||||||||||||||||| ||||||||||||||||||
Sbjct 1256 CCGAAGATAACTCCATGAATTTCTTCGCGATTCAAAGCTTTGGTCACTTCATTCGCGGCG 1197
Query 637 AGGATGATAAAGTTAACGAGTTAGATTCTGAATTTTTGGGTAAATTGGAAGCGGAAAAGA 696
|||||||||| |||||||| ||||||||| ||||||||||||||||||||||||| ||||
Sbjct 1196 AGGATGATAAGGTTAACGATTTAGATTCTCAATTTTTGGGTAAATTGGAAGCGGAGAAGA 1137
Query 697 CTAGCGTCGCTGAAACTATCTCTGGTTGTGAGAAAATCTCCGGTGAATTGGAGGCTAAAC 756
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1136 CTAGCGTCGCTGAAACTATCTCTGGTTGTGAGAAAATCTCCGGTGAATTGGAGGCTAAAC 1077
Query 757 TTGAGTCACTGAGGAAAGGTCCGTCGAAGAAAGAGTCTCTGGGGAAAGCTAAAGCCGATT 816
|||||||||||||||||||||| ||||||||||||||||||| ||||| |||||||||||
Sbjct 1076 TTGAGTCACTGAGGAAAGGTCCTTCGAAGAAAGAGTCTCTGGAGAAAGTTAAAGCCGATT 1017
Query 817 TGGAGAATGACGTGAACAAGTTCCGAACTATTGTTGCGGAATATACTGAAAAGACTCAAG 876
||||||||||||| |||||||||||||||||||||| ||| |||||||| | || || ||
Sbjct 1016 TGGAGAATGACGTAAACAAGTTCCGAACTATTGTTGTGGAGTATACTGATAGGAATCCAG 957
Query 877 CGCTGGAGAAAGTTGTGGAGGAGAAAGTTAAGGAGCTTAAAGCTAAGgaagaagagagag 936
|| |||||||||||||||||||||||| ||||||||| ||||||||||||||||||||||
Sbjct 956 CGATGGAGAAAGTTGTGGAGGAGAAAGCTAAGGAGCTCAAAGCTAAGGAAGAAGAGAGAG 897
Query 937 agcgaatctctgaagagaataaagagttgaagaagagTGTGGACCTTCAGAACTTTAGCG 996
|||||||||||| ||||||||| |||||||||||||||||||| ||||||||||||||||
Sbjct 896 AGCGAATCTCTGTAGAGAATAAGGAGTTGAAGAAGAGTGTGGAGCTTCAGAACTTTAGCG 837
Query 997 CCAGAG-ATGTAGAGAGGATGAGAAGAGAGTTACAAGCTGTGGAAAGAGATGTTGCAGAG 1055
| | | ||||| | ||||||||||||||||||||||||||||||||||||||||| ||
Sbjct 836 CG-GCGGATGTAAATAGGATGAGAAGAGAGTTACAAGCTGTGGAAAGAGATGTTGCGGAT 778
Query 1056 GCTGAGGTTGCTAGAGATGGTTGGGATCTGAAAGCTTGGGAACTCAATTCACAGATTGGG 1115
|||||||||||||||||||||||||||| |||||||||||||||||||||||||||| ||
Sbjct 777 GCTGAGGTTGCTAGAGATGGTTGGGATCAGAAAGCTTGGGAACTCAATTCACAGATTAGG 718
Query 1116 AATCAGTTTCATCAGATTCAGACACTTTCCATTGATTGTAACCAAGTTCTTAGGAGATTG 1175
||||||||||||||||||||||||||| ||||||| || ||||||| ||| |||||||||
Sbjct 717 AATCAGTTTCATCAGATTCAGACACTTGCCATTGACTGCAACCAAGCTCTCAGGAGATTG 658
Query 1176 AAGCTTGATATTCAGTTCACTGTAAATGAGAGAGGGAAAACACCTGCAAAGGTGATTGGT 1235
|||||||||||||||||| |||| || ||||||||| ||||||||||| |||||| |||
Sbjct 657 AAGCTTGATATTCAGTTCGCTGTTAACGAGAGAGGGGAAACACCTGCAGCGGTGATGGGT 598
Query 1236 GTGGATTATAAATCTGTGGTGAAGCCAGCTCTGTGTTCGCTGTACGATGGGATTAAAGGA 1295
|||||||||||||| |||||||||||||||||||||||| ||| ||||||||| ||||||
Sbjct 597 GTGGATTATAAATCGGTGGTGAAGCCAGCTCTGTGTTCGTTGTGCGATGGGATCAAAGGA 538
Query 1296 AGCTTGACGGAGAAAATGGAGGAGTTGGTCACTTTACAGCAAGAGGTGTCTGAAATGGCT 1355
|||| | |||||||| ||||||| ||||||||||||||||| | |||||||||||||
Sbjct 537 AGCTCGGCGGAGAAAGTGGAGGAATTGGTCACTTTACAGCACCATAAGTCTGAAATGGCT 478
Query 1356 TCGAAGATCGAATCCAAAAAGAGTCTTCTTGGGTCAATTCAGTTGCAGATCAATGAACTC 1415
||||||||||||||||||| |||||||||||||||||||||||||||||||||||| |||
Sbjct 477 TCGAAGATCGAATCCAAAAGGAGTCTTCTTGGGTCAATTCAGTTGCAGATCAATGACCTC 418
Query 1416 GAAGAAAAGATGAAGTTGGTGAAGAAGGAAACTCAGGAGTTGACTACAAAATGTGACTTG 1475
||||||||||||||||||||||||||||||||||||||||||| |||||||||||| |||
Sbjct 417 GAAGAAAAGATGAAGTTGGTGAAGAAGGAAACTCAGGAGTTGAGTACAAAATGTGATTTG 358
Query 1476 GAAGCGAAAACATTGGTTGAGAGTGTGAAAACGGAGGCTTTGAATCTGGAGGTTGTGGAG 1535
|| ||||||||||||||||||||||||||| | |||||||||||||||||||||||||||
Sbjct 357 GAGGCGAAAACATTGGTTGAGAGTGTGAAAGCTGAGGCTTTGAATCTGGAGGTTGTGGAG 298
Query 1536 AAAGAAGCTGCTGAGTTTTTAAAAGCTTCGGAGGTAAGATTGCAAGAAGCAGAAAAGCAG 1595
|||||||||||||||||| |||||||||||||| | |||||||||||||||| ||| ||
Sbjct 297 AAAGAAGCTGCTGAGTTTGTAAAAGCTTCGGAGCTTAGATTGCAAGAAGCAGTGAAGGAG 238
Query 1596 AGTGAAGAGGAGGTCCAAGCATGTGCTGCACAACTATTTGCACTTATCGATTCGATATCG 1655
||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
Sbjct 237 AGTGAAGAGGAGGTCCAAGCATGTGCTGCACAACTCTTTGCACTTATCGATTCGATATCG 178
Query 1656 AAACAGAAAGAGTACATGGATTTGAAGATATTGGAGATCAAAACTGGTGTTGCTGATACT 1715
|||||||||||||||||||||| |||||||| |||||||||||| |||||||||||||||
Sbjct 177 AAACAGAAAGAGTACATGGATTCGAAGATATCGGAGATCAAAACCGGTGTTGCTGATACT 118
Query 1716 GCCAGTGCT 1724
|||||||||
Sbjct 117 GCCAGTGCT 109
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 90059777730
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5