BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg486833
Length=1955
Score E
Sequences producing significant alignments: (Bits) Value
AT3G63280.1 | Symbols: ATNEK4, NEK4 | NIMA-related kinase 4 | c... 1984 0.0
AT1G54510.1 | Symbols: ATNEK1, NEK1 | NIMA-related serine/threo... 459 6e-128
> AT3G63280.1 | Symbols: ATNEK4, NEK4 | NIMA-related kinase 4 |
chr3:23377979-23381546 FORWARD LENGTH=2066
Length=2066
Score = 1984 bits (1074), Expect = 0.0
Identities = 1214/1280 (95%), Gaps = 15/1280 (1%)
Strand=Plus/Plus
Query 1 GTAGAGGGGAAATTGTTCTTTTGCCGCTCTCGGACGAATCGT-T-TGAATTTGATTCCCT 58
||||| |||||||||||||||||||||||||||||||||||| | || | || | | |
Sbjct 55 GTAGACGGGAAATTGTTCTTTTGCCGCTCTCGGACGAATCGTCTGTGTA-TT-AGTAGTT 112
Query 59 TG-TGCTCATCCACCACGGTGTCGGCACGATTGAAGCTCCTCCCTCCAAGAGCCGTCGTC 117
|| | ||| | |||||||||||||| |||||||||||||||||||||||||||| ||||
Sbjct 113 TGATTCTC-TTCACCACGGTGTCGGTACGATTGAAGCTCCTCCCTCCAAGAGCCTTCGTA 171
Query 118 CAGAGACGTCCCTGGTGAAATTTCAAGCACGGGAAGTGTGTGTTTGTTAGATGGAGCGGT 177
||||||||| ||| |||||||||||| | || |||||||||| ||||||||||||
Sbjct 172 CAGAGACGTTTCTGCTGAAATTTCAAG--C---AA--GTGTGTTTGTCAGATGGAGCGGT 224
Query 178 ATGAAGTCTTGGAGCAGATCGGGAAAGGGTCCTTTGGCTCTGCTCTTCTTGTCAGACACA 237
|||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
Sbjct 225 ATGAAGTCTTGGAGCAGATCGGGAAAGGGTCCTTTGGCTCTGCTCTTCTTGTTAGACACA 284
Query 238 AACAAGAACGCAACAAATACGTTTTGAAAAAGATTCGTCTCGCCCGCCAGTCTGATAGGG 297
||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 285 AACAAGAACGCAAGAAATACGTTTTGAAAAAGATTCGTCTCGCCCGCCAGTCTGATAGGG 344
Query 298 CTCGCCGATCAGCTCATCAGGAGATGGAGCTCATTTCTACTGTCCGCAATCCATTTGTTG 357
|||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 345 CTCGACGATCAGCTCATCAGGAGATGGAGCTCATTTCTACTGTCCGCAATCCATTTGTTG 404
Query 358 TCGAGTACAAAGATTCATGGGTTGAAAAGGGTTGCTATGTGTGCATCGTTATTGGATATT 417
|||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
Sbjct 405 TCGAGTACAAAGATTCATGGGTTGAAAAGGGTTGCTATGTGTGCATTGTTATTGGATATT 464
Query 418 GTGAAGGCGGAGACATGACAGAGACCATAAAAAGAGCATGTGGTGTTCATTTTCCTGAAG 477
| ||||||| ||||||||||| ||||| ||||||||||||||||||||||||||||| |
Sbjct 465 GCCAAGGCGGGGACATGACAGATACCATCAAAAGAGCATGTGGTGTTCATTTTCCTGAGG 524
Query 478 AGAAACTCTGCCAATGGCTTGTCCAACTCTTAATGGCACTCGATTATCTTCACTCCAACC 537
||||| | |||||||||||||||||||| |||||||| ||||||||||||||||||||||
Sbjct 525 AGAAATTATGCCAATGGCTTGTCCAACTATTAATGGCCCTCGATTATCTTCACTCCAACC 584
Query 538 ATATTCTTCACCGCGATGTCAAGTGTTCTAACATATTCTTGACAAAAGAGCAAGACATAC 597
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 585 ATATTCTTCACCGCGATGTCAAGTGTTCTAACATATTCTTGACAAAAGAGCAAGACATAC 644
Query 598 GGCTTGGTGACTTTGGACTCGCTAAGATTCTAACTTCTGATGACCTTACATCCTCTGTTG 657
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 645 GGCTTGGTGACTTTGGACTCGCTAAGATTCTAACTTCTGATGACCTTACATCCTCTGTTG 704
Query 658 TTGGAACTCCAAGCTACATGTGTCCTGAGCTTCTTGCTGACATACCTTACGGATCAAAGT 717
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 705 TTGGAACTCCAAGCTACATGTGTCCTGAGCTTCTTGCTGACATACCTTACGGATCAAAGT 764
Query 718 CAGACATTTGGTCTTTGGGATGCTGCATGTATGAAATGGCTGCTCATAAACCTCCATTCA 777
||||||||||||| ||||||||||||||||||||||||||||||||||||||||| ||||
Sbjct 765 CAGACATTTGGTCGTTGGGATGCTGCATGTATGAAATGGCTGCTCATAAACCTCCTTTCA 824
Query 778 AAGCTTCCGATGTGCAGACATTGATCACCAAAATACACAAGTTGATAATGGATCCTATTC 837
|||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 825 AAGCTTCCGATGTGCAGACACTGATCACCAAAATACACAAGTTGATAATGGATCCTATTC 884
Query 838 CGGCCATGTATTCCGGCTCATTCCGAGGCCTTATCAAAAGCATGTTGAGGAAAAATCCTG 897
| |||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 885 CCGCCATGTATTCCGGCTCATTCCGAGGCCTTATTAAAAGCATGTTGAGGAAAAATCCTG 944
Query 898 AACTCAGGCCAAGTGCTAACGAGCTGCTTAACCATCCTCATCTTCAGCCTTACATCAGTA 957
||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 945 AACTCAGGCCAAGCGCTAACGAGCTGCTTAACCATCCTCATCTTCAGCCTTACATCAGTA 1004
Query 958 TGGTTTATCTGAAGCTAGAGAGCCCCAGAAGAAGCACTTATCCACTCCAATTTTCTGAGA 1017
|||||||| |||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 1005 TGGTTTATATGAAGCTAGAGAGCCCCAGAAGAAGCACTTTTCCACTCCAATTTTCTGAGA 1064
Query 1018 GTTG-CGAGACAGAGAAGGAAAGAAGGCGTTCATCATTCAGCAATGACAGGAGATTGAAT 1076
| | || ||| |||||||||||||||||||||||||||||| |||||||||||||||
Sbjct 1065 GG-GACGCTACATTGAAGGAAAGAAGGCGTTCATCATTCAGCAACGACAGGAGATTGAAT 1123
Query 1077 CCGAGTGTCTCTGATACAGAAGCAGGTTCTGTTTCTTCTTCAGGAAAAGCATCTCATTCA 1136
||||||||||||||||||||||||||||||||||||||||||||||||||||||| | ||
Sbjct 1124 CCGAGTGTCTCTGATACAGAAGCAGGTTCTGTTTCTTCTTCAGGAAAAGCATCTCCTACA 1183
Query 1137 CCAATGTTTAATGGAAGAAAAGTGTCAGAAGTAACTGTGGGAGTCGTGAGAGAAGAAATT 1196
||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
Sbjct 1184 CCAATGTTTAATGGAAGAAAAGTGTCAGAAGTAACTGTGGGAGTCGTCAGAGAAGAAATT 1243
Query 1197 GTTCCTCAAAGGCAAGAGGGAGTGAAGAAGCAATCAAGTGCAGCCAGAACACCAAGAGTG 1256
||||||||| ||||||||| || |||||||||||| |||||||||||||||||||||||
Sbjct 1244 GTTCCTCAACGGCAAGAGGAAGCCAAGAAGCAATCAGGTGCAGCCAGAACACCAAGAGTG 1303
Query 1257 GCTGGGACCTCAGCTAAAGC 1276
||||||||||||||||||||
Sbjct 1304 GCTGGGACCTCAGCTAAAGC 1323
Score = 839 bits (454), Expect = 0.0
Identities = 603/671 (90%), Gaps = 25/671 (4%)
Strand=Plus/Plus
Query 1309 CAAGCACGCCGCGTACTGTTTCCAAACATGAAGTGATTAAGGTATCGAATCCTACAGACA 1368
||||||||| ||||||||||| ||||||||| | || |||||||||||||||||||| |
Sbjct 1323 CAAGCACGCAGCGTACTGTTTTTAAACATGAACTAATGAAGGTATCGAATCCTACAGAGA 1382
Query 1369 GGAGAAGGAGAGTATCTCTGCCGTTGGCCGTGGAAAATCCTTACATTTACGAATCGGATA 1428
||||||||||||||||||||||||||| ||||||||||||||||| ||| ||||| ||||
Sbjct 1383 GGAGAAGGAGAGTATCTCTGCCGTTGGTCGTGGAAAATCCTTACACTTATGAATCAGATA 1442
Query 1429 TTACTGCTCTTTGTGGCCTGAATTCACCGGATGTTTCTGTAAACGCTCCAAGAATCGACA 1488
|||| ||||||||| ||| |||||||||||||||||||||||| |||||||| ||||||
Sbjct 1443 TTACAGCTCTTTGTTCCCTAAATTCACCGGATGTTTCTGTAAACACTCCAAGATTCGACA 1502
Query 1489 AGATCGCTGAATTCCCTGAAGATGTCTTCCAAAACCAAAACAGAGAGACAGCCTC---AA 1545
||||||||||||||||||||||| |||||||||||||||||||||||||||| || ||
Sbjct 1503 AGATCGCTGAATTCCCTGAAGATATCTTCCAAAACCAAAACAGAGAGACAGCGTCAAGAA 1562
Query 1546 GAGCAGTAGCTAGGCGCTCTTTTTCTTCTACTCC---TCGGCCTCATGGTGAGGACAATA 1602
||| ||||||||||| ||||||||||||| |||| || |||||||||||||||||||
Sbjct 1563 GAGAAGTAGCTAGGCACTCTTTTTCTTCTCCTCCTTGTCCGCCTCATGGTGAGGACAATT 1622
Query 1603 CCAATC-GTTCGATCACGAAGGACAAATGTACTGTACAGACAAGGTCAGTTTCGGAGGTA 1661
|||| | |||||||||| |||||||| ||||||||||||| |||||||||||||||||||
Sbjct 1623 CCAA-CGGTTCGATCACAAAGGACAAGTGTACTGTACAGAAAAGGTCAGTTTCGGAGGTA 1681
Query 1662 AAACAGAGAAGGTTCGACACGTCATCGTATCAGCAGCGTGCGGAGGCTCTGGAGGGACTG 1721
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1682 AAACAGAGAAGGTTCGACACGTCATCGTATCAGCAGCGTGCGGAGGCTCTGGAGGGACTG 1741
Query 1722 CTGGAGTTCAGTGCAAGGCTTTTGCAGCAAGAACGATACGATGAGCTTGGGGTTCTGCTT 1781
|||||||||||||||| |||||||||||| ||||| ||||||||||| ||||||||||||
Sbjct 1742 CTGGAGTTCAGTGCAAAGCTTTTGCAGCAGGAACGGTACGATGAGCTCGGGGTTCTGCTT 1801
Query 1782 AAACCCTTTGGAGCCGAGAGGGTATCTTCGAGGGAGACGGCCATCTGGTTGACCAAGAGC 1841
||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
Sbjct 1802 AAACCCTTTGGAGCCGAGAGGGTGTCTTCGAGGGAGACGGCCATCTGGTTGACCAAGAGC 1861
Query 1842 TTCAAAGAAGCTTCCGTGTAGACGAAGCAACCA---CAA-C-CT-TGGCT-TGCAT-T-T 1892
|||||||||||||||||||||| |||||||||| ||| | || | || | | | | |
Sbjct 1862 TTCAAAGAAGCTTCCGTGTAGATGAAGCAACCATAACAAACGCTCTATCTCTTCTTCTCT 1921
Query 1893 -GTC---CTT--G-C-TGTGTGGTTTGTATATAAATAAAAACGGCGATGCGTTCGGTTCT 1944
| | ||| | | ||||||||||||||||||||||||||| ||| || |||||||||
Sbjct 1922 TGGCTTGCTTTTGTCCTGTGTGGTTTGTATATAAATAAAAACGACGACGCATTCGGTTCT 1981
Query 1945 GACTGCTTAAA 1955
| ||||||||
Sbjct 1982 CATTGCTTAAA 1992
> AT1G54510.1 | Symbols: ATNEK1, NEK1 | NIMA-related serine/threonine
kinase 1 | chr1:20358319-20362545 REVERSE LENGTH=2254
Length=2254
Score = 459 bits (248), Expect = 6e-128
Identities = 627/805 (78%), Gaps = 46/805 (6%)
Strand=Plus/Plus
Query 168 ATGGAGCGGTATGAAG--TCTTGGAGCAGATCGGGAAAGGGTCCTTTGGCTCTGCTCTTC 225
||||| | ||||| || |||| ||||| || || ||||| || ||||| || ||||||
Sbjct 132 ATGGAACAGTATG-AGTTTCTT-GAGCAAATTGGAAAAGGATCTTTTGGGTCAGCTCTT- 188
Query 226 TT-GTCAGACACAAACA--AGAACGCAACAAATACGTTTTGAAAAAGATTCGTCTCGCCC 282
|| || ||||| || || |||| | || ||||| || ||||||||||| ||||| ||||
Sbjct 189 TTAGTGAGACATAAGCATGAGAA-G-AAAAAATATGTATTGAAAAAGATCCGTCTTGCCC 246
Query 283 GCCAGTCTGATAGGGCTC-GCCGATCAGCTCATCAGGAGATGGAGCTCATTTCTACTGTC 341
| || || | ||| | | | ||||| || ||||| ||||||||||| || || | |
Sbjct 247 GGCAAACTCAAAGGAC-CAGACGATCTGCACATCAAGAGATGGAGCTTATCTCGAAAATG 305
Query 342 CG-CAATCCATTTGTTGTCGAGTACAAAGATTCATGGGTTGAAAAGGGTTGCTATGTGTG 400
|| | |||||||| | || || |||||||| || ||||| ||||||| ||||||||||||
Sbjct 306 CGTC-ATCCATTTATCGTGGAATACAAAGACTCTTGGGTGGAAAAGGCTTGCTATGTGTG 364
Query 401 CATCGTTATTGGATATTGTGAAGGCGGAGACAT-G-ACAGAGACCAT-AA-AAAGAGCAT 456
||| || || || ||||||||||| |||||||| | ||| || | || || ||| ||
Sbjct 365 CATTGTCATAGGTTATTGTGAAGGAGGAGACATGGCACA-AG-CTATCAAGAAA-TCCA- 420
Query 457 GTGGTGTTCATTTTCCTGAAGAGAAACTCTGCCAA-TGGCTTGTCCAACTCTTAAT-GGC 514
|||||| ||||||| ||||||||||| || ||| |||||||| || ||| | || |||
Sbjct 421 ATGGTGTGCATTTTCAGGAAGAGAAACTGTG-CAAGTGGCTTGTTCAGCTCCTGATGGGC 479
Query 515 ACTCGATTATCTT-CACTCCAACCATATTCTTCACCGCGATGTCAAGTGTTCTAACATAT 573
|| || || ||| || || |||||||||||||| || ||||||||||||||||||||||
Sbjct 480 -CTTGAGTA-CTTACATTCTAACCATATTCTTCATCGTGATGTCAAGTGTTCTAACATAT 537
Query 574 TCTTGACAAAAGAGCAAGACATACGGCTTGGTGACTTTGGACTCGCTAAGATTCTAACTT 633
||||||| ||||||||||| ||||| |||||||| ||||| | || ||||||||||| |
Sbjct 538 TCTTGACCAAAGAGCAAGATATACGCCTTGGTGATTTTGGGTTAGCCAAGATTCTAACAT 597
Query 634 CTGATGACCTTACATCCTCTGTTGTTGGAACTCCAAGCTACATGTGTCCTGAGCTTCTT- 692
| || || || |||||||||||||| || ||||| ||||| ||||| ||||||||| ||
Sbjct 598 CCGACGATCTCACATCCTCTGTTGTCGGGACTCCCAGCTATATGTGCCCTGAGCTT-TTA 656
Query 693 GCTGACATACCTTACGGATC-AAAGTCAGACATTTGGTCTTT-GGGATGCTGCATGTATG 750
||||| |||||||| || || ||| || |||||||||||||| ||| ||||| || ||||
Sbjct 657 GCTGATATACCTTATGGTTCCAAA-TCTGACATTTGGTCTTTAGGG-TGCTGTATATATG 714
Query 751 AAATGGCTGCTCATAAACCTCCATTCAAAGC-TTCCGATGTGCA-GACATTGATCACCAA 808
|||||||| || ||| || |||| ||||| || ||| |||| | || |||| | ||
Sbjct 715 AAATGGCTTATCTTAAGCCGGCATTTAAAGCATTT-GATATGCAAG-CACTGATTAATAA 772
Query 809 AATACACAAGTTGATAATGGATCCTATTCCGGCCATGTATTCCGGCTC-ATTCCGAGGCC 867
||| ||||| ||| || ||| | || |||| ||||| || || ||||||||| |
Sbjct 773 GATAAACAAGACGATTGTGTCTCCGCTCCCTGCCAAATATTCTGG-TCCATTCCGAGGTC 831
Query 868 TTATCAAAAGCATGTTGAGGAAAAATCCTGAACT-CAGGCCAAGTGC-TAACGAGCTGCT 925
| ||||||||||| || ||||||| || ||| | | ||||| ||| | | || | ||
Sbjct 832 TCGTCAAAAGCATGCTGCGGAAAAACCCAGAAGTTC-GGCCATCTGCCTCA-GATTTACT 889
Query 926 TA-ACCATCCTCATCTTCAGCCTTA 949
|| || |||| ||||||||||||||
Sbjct 890 TAGAC-ATCCACATCTTCAGCCTTA 913
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 96836851305
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5