BLASTN 2.2.26+ Reference: Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000), "A greedy algorithm for aligning DNA sequences", J Comput Biol 2000; 7(1-2):203-14. Database: TAIR10_cdna_20110103_representative_gene_model_updated 33,602 sequences; 51,074,197 total letters Query= Ahg487217 Length=1379 Score E Sequences producing significant alignments: (Bits) Value AT5G04370.2 | Symbols: NAMT1 | S-adenosyl-L-methionine-dependen... 2100 0.0 AT5G66430.1 | Symbols: | S-adenosyl-L-methionine-dependent met... 774 0.0 AT3G11480.1 | Symbols: BSMT1, ATBSMT1 | S-adenosyl-L-methionine... 686 0.0 > AT5G04370.2 | Symbols: NAMT1 | S-adenosyl-L-methionine-dependent methyltransferases superfamily protein | chr5:1231697-1234147 FORWARD LENGTH=1666 Length=1666 Score = 2100 bits (1137), Expect = 0.0 Identities = 1307/1388 (94%), Gaps = 16/1388 (1%) Strand=Plus/Plus Query 1 ACTAAGTTTTTTCTTGTATAGAAACACTCGTAATTCTAATTTCCCACTATTTCCtttttt 60 |||||||||| |||| ||||||||||||||| |||||||||| ||||||| ||| || Sbjct 35 ACTAAGTTTTCTCTTATATAGAAACACTCGTTATTCTAATTTGCCACTATATCC---ATT 91 Query 61 ttttGCTTATTTATTTATTTGACAAAATTTTAAATTATATATAATCTCTAGAAAGATTGG 120 |||||||||||||||||||||| | ||||||||||||| |||||||| ||||||||| Sbjct 92 TTTTGCTTATTTATTTATTTGA-ATTTTTTTAAATTATAT-TAATCTCTGGAAAGATTGC 149 Query 121 AAGATTGGGCATGATTGAATGTGCAACTGTGTATTGCAACAACTCGGCGTGTTTCACGTT 180 ||||||||| ||||||||||||| |||||||||| ||||||||||||||||||||||||| Sbjct 150 AAGATTGGGTATGATTGAATGTGGAACTGTGTATCGCAACAACTCGGCGTGTTTCACGTT 209 Query 181 CAAGATA--T----GTCCCACCTCTATAAATAAAACGACTGAGAGGTGTAATGGTAAGAG 234 ||||||| | |||||||||||||||||||||||||| |||| |||||||||||||| Sbjct 210 CAAGATATGTCCCAGTCCCACCTCTATAAATAAAACGACTAAGAGATGTAATGGTAAGAG 269 Query 235 TGAAAATGAAATGAAAAACGGCGATGAAGAAAGAATTAGTAAATACT-CTTATGTGAGTC 293 ||||||||| | ||||||||||||||||||||||||||||||||||| ||| |||||||| Sbjct 270 TGAAAATGAGAGGAAAAACGGCGATGAAGAAAGAATTAGTAAATACTCCTT-TGTGAGTC 328 Query 294 CTCTATGTATGAGTGGAGGAGATGGATACAACAGTTACTCCACCAATTCTCTTCTTCAGA 353 | || |||||| ||||||||||||||||||| |||||||||| ||||||||||||||||| Sbjct 329 CACTGTGTATGCGTGGAGGAGATGGATACAATAGTTACTCCAGCAATTCTCTTCTTCAGA 388 Query 354 GAAGAGTGTTATCAAAGGCTAAACCGGTATTGGTTAAGAACACAAAGGAACTGATGATAA 413 ||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||| Sbjct 389 GAAGAGTGTTATCAAAGGCTAAACCGGTATTGGTTAAGAACACAAAGGACCTGATGATAA 448 Query 414 ACTTAAACTTTCCTACATACATTAAAGTAGCTGATTTGGGTTGTTCATCTGGACAAAACA 473 |||||||||||||||||||||||||||||||||||||||| |||||||| ||||| || | Sbjct 449 ACTTAAACTTTCCTACATACATTAAAGTAGCTGATTTGGGCTGTTCATCAGGACAGAATA 508 Query 474 CATTCTTGGCGATGTCTGAAATTATCAATACAGTCAATGTGTTATGTCAACAGAGGAACC 533 | |||||||| ||||||||||||||||||||| |||||||||| |||||||||||||||| Sbjct 509 CGTTCTTGGCAATGTCTGAAATTATCAATACAATCAATGTGTTTTGTCAACAGAGGAACC 568 Query 534 AAAACCCACCAGAAATAGATTGTTGTCTAAATGATCTCCCTAATAACGATTTTAACACAA 593 |||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||| Sbjct 569 AAAACCCACCAGAAATAGATTGTTGTCTAAATGATCTCCCTAGTAACGATTTTAACACAA 628 Query 594 CGTTCAAATTCATACAATTCTTCAACGAGAAGAACATCACGAGCAAAGAATCATACTTTG 653 ||||||||||||||||||||||||| | || ||||||||||||||||||||||||||||| Sbjct 629 CGTTCAAATTCATACAATTCTTCAATGGGATGAACATCACGAGCAAAGAATCATACTTTG 688 Query 654 TCTCTGGAGTCCCCGGTTCTTTCTACTCGAGGCTCTTCCCTCGGAGGAGTCTTCATTTCG 713 ||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 689 TCTATGGAGTCCCCGGTTCTTTCTACTCGAGGCTCTTCCCTCGGAGGAGTCTTCATTTCG 748 Query 714 TACATTCCTCTTATGGTCTCCATTGGCTCTCTAAGGTTCCTGAAGGGCTTAAGAAGAGTA 773 |||||||||||||||||||||||||||||||||||||||||||||||||| |||||| || Sbjct 749 TACATTCCTCTTATGGTCTCCATTGGCTCTCTAAGGTTCCTGAAGGGCTTGAGAAGAATA 808 Query 774 AGATGAGTGTGTACATCACAAATTCAAGTCCTCTAAGTACATATAAGGCTTACTTAAATC 833 ||||||||||||||||||||||||||||||||||||| ||||| |||||||||||||||| Sbjct 809 AGATGAGTGTGTACATCACAAATTCAAGTCCTCTAAGCACATACAAGGCTTACTTAAATC 868 Query 834 AATTCCAAACAGATTTCGCGACATTTCTTAAACTGCGTTCTGAAGAGATGGTTTCTAATG 893 ||||||||| |||||| |||||||| |||||||||||||||||||||||||||||||||| Sbjct 869 AATTCCAAAGAGATTTTGCGACATTCCTTAAACTGCGTTCTGAAGAGATGGTTTCTAATG 928 Query 894 GACGCATGGTTCTCACTTTCATTGGTCGAAACACTATTGATAATCCATTGCATAGAGATT 953 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 929 GACGCATGGTTCTCACTTTCATTGGTCGAAACACTATTGATAATCCATTGCATAGAGATT 988 Query 954 GTTGTCACTTTTGGACATTATTATCCAGATCTCTCCGTGACCTAGTCGTCGAGGGTCTTG 1013 ||||||||||||||||||||||||||| |||||| ||||||||||||| |||||| |||| Sbjct 989 GTTGTCACTTTTGGACATTATTATCCAAATCTCTTCGTGACCTAGTCGCCGAGGGCCTTG 1048 Query 1014 TGAGTCCATCGAAGGTAGATTCGTTCTACATT-CCATTTTATGACCCCAACGAAAAAGAA 1072 ||||| |||| ||||||||||| |||||| || || |||||||||||||||||||||||| Sbjct 1049 TGAGTGCATCAAAGGTAGATTCATTCTAC-TTACCGTTTTATGACCCCAACGAAAAAGAA 1107 Query 1073 GTAAAGGAGGTGGTACAGAAAGAAGGTTCCTTTGAAATCAAAGACTTGGAGACACATGGA 1132 |||||||| |||||||||||||||||||||||||||||| ||||||||||||||||||| Sbjct 1108 ATAAAGGAGATGGTACAGAAAGAAGGTTCCTTTGAAATCAGAGACTTGGAGACACATGGA 1167 Query 1133 TATGACCTTGGCCATTGTAACCAAGACGAGTCAAAGAGAAGTAAATCAGGGAAAAATGAA 1192 ||||| ||||||||||||||||||||||||||||||||||||||||||||| |||||||| Sbjct 1168 TATGATCTTGGCCATTGTAACCAAGACGAGTCAAAGAGAAGTAAATCAGGGCAAAATGAA 1227 Query 1193 GCCAATTACATCAGAGCGGTGAGTGAACCACTGCTCGTAGCTCACTTTGGAGATGCCATC 1252 || |||||||||||||||||||||||||||||||| | |||||||||||||||||||| Sbjct 1228 GCTAATTACATCAGAGCGGTGAGTGAACCACTGCTTGCGGCTCACTTTGGAGATGCCATT 1287 Query 1253 ACCAACATATTGTTTAACAAGTTTGCATGTCATGTGTCTCAACATGCTAGTTGCAGGAAC 1312 | |||||||||||||||||||||||||||||||||||||||||||| ||||||||||||| Sbjct 1288 ATCAACATATTGTTTAACAAGTTTGCATGTCATGTGTCTCAACATGTTAGTTGCAGGAAC 1347 Query 1313 AAAACGACCGTCAGCATAGTCGTTTCATTGACTAAGAAAAAA-CTTTCCTCATGTCCTAA 1371 ||||||||||||||||||||||||||| |||||||||||||| ||||||||||||||||| Sbjct 1348 AAAACGACCGTCAGCATAGTCGTTTCACTGACTAAGAAAAAAACTTTCCTCATGTCCTAA 1407 Query 1372 AGTTTAAT 1379 |||||||| Sbjct 1408 AGTTTAAT 1415 > AT5G66430.1 | Symbols: | S-adenosyl-L-methionine-dependent methyltransferases superfamily protein | chr5:26525410-26526799 REVERSE LENGTH=1065 Length=1065 Score = 774 bits (419), Expect = 0.0 Identities = 868/1080 (80%), Gaps = 49/1080 (5%) Strand=Plus/Plus Query 302 ATGAGTGGAGGAGATGGATACAACAGTTACTCCACCAATTCTCTTCTTCAGAGAAGAGTG 361 ||||| || ||||||||| | || |||||||||||||||||||||||||||| || ||| Sbjct 1 ATGAGCGGTGGAGATGGAGATAATAGTTACTCCACCAATTCTCTTCTTCAGAAAAAAGTA 60 Query 362 TTATCAAAGGCTAAACCGGTATTGGTTAAGAACAC-AAAGGAACTGATGATAAACTTAAA 420 ||||||||||| |||||||| |||||||||||||| ||||||| |||||||||||||||| Sbjct 61 TTATCAAAGGCCAAACCGGTTTTGGTTAAGAACACAAAAGGAA-TGATGATAAACTTAAA 119 Query 421 CTTTCCTACA-TACATTAAAGTAGCTGATTTGGGTTGTTCAT-CTGGACAAAACACATTC 478 ||||||| | ||||| ||||||||||||||||| ||| | | ||||| ||||||| ||| Sbjct 120 TTTTCCTA-ATTACATCAAAGTAGCTGATTTGGGATGTGC-TACTGGAGAAAACACGTTC 177 Query 479 TTGGCGATGTCTGAAATTATCAATACAG-TCAATGTGTTATGTCAACAGA-GGAACCAAA 536 ||| ||||| |||| || | || || | ||||||||||||||||||| | | ||||||| Sbjct 178 TTGACGATGGCTGAGATCGTGAACAC-GATCAATGTGTTATGTCAACA-ATGTAACCAAA 235 Query 537 ACCCACCAGAAATAGATTGTTGTCTAAATGATCTCCCTAATAACGATTTTAACACAACGT 596 | ||||||||||| ||||| ||||| |||||||| || |||||||||| ||||| || | Sbjct 236 AGCCACCAGAAATTGATTGCTGTCTGAATGATCTTCCGGATAACGATTTCAACACGACAT 295 Query 597 TCAAATTCATACAATTCTTCAACGAGAAGAACATCACGAGC-AAAGAATCATACTTTGTC 655 |||| ||| ||| |||||||| | || | ||| ||| |||| || | ||| || Sbjct 296 TCAAGTTCGTACCTTTCTTCAA-TA-AA-AGGGTCAAAAGCAAAAG-ATTGTGCTTCGTG 351 Query 656 TCTGGAGTCCCCGGTTCTTTCTACTCGAGGCTCTTCCCTCGGAGGAGTCTTCATTTCGTA 715 ||||||||||||||||| ||||||||||||||||| ||||| | ||||| ||||| ||| Sbjct 352 TCTGGAGTCCCCGGTTCATTCTACTCGAGGCTCTTTCCTCGCAAAAGTCTCCATTTTGTA 411 Query 716 CATTCCTCTTATGGTCTCCATTGGCTCTCTAAGGTTCCTGAAGGGCTTAAGAAGAGTAAG 775 ||||| |||||| | ||||||||||||||||||||||| |||| || |||||| | Sbjct 412 CATTCATCTTATAGCCTCCATTGGCTCTCTAAGGTTCCCAAAGGACTGGAGAAGAAT-TC 470 Query 776 AT-GAGTGTGTACATCACAAATTCAAGTCCTCTAAGTACATATAAGGCTTACTTAAATCA 834 | | ||||||||| || | ||||||||||| || | |||| |||||||| |||||||| Sbjct 471 CTCGTCTGTGTACATAACGACTTCAAGTCCTCCAAATGCATACAAGGCTTATTTAAATCA 530 Query 835 ATTCCAAACAGA-TTTCGCGACATTTCTTA-AACTGCGTTCTGAAGAGATGGTTTCTAAT 892 |||||||| || ||| | |||||| || || |||||||||| ||||||||||||||| Sbjct 531 ATTCCAAAGTGACTTT-AAGTCATTTC-TAGAAATGCGTTCTGAGGAGATGGTTTCTAAT 588 Query 893 GGACGCATGGTTCTCACTTTCATTGGTCGAAACACTATTGATAATCCATTGCATAGAGAT 952 ||||||||||||||||||||||| ||| |||| ||| | ||| ||||||||||||||||| Sbjct 589 GGACGCATGGTTCTCACTTTCATCGGTAGAAAGACTCTCGATGATCCATTGCATAGAGAT 648 Query 953 TGTTGTCACTTTTGGACATTATTATCCAGATCTCTCCGTGACCTAGTCG-TCGAGGGTCT 1011 |||||||||||||||||| ||||||| | |||||| || ||||||||| | |||||||| Sbjct 649 TGTTGTCACTTTTGGACACTATTATCTACATCTCTACGCGACCTAGTCTAT-GAGGGTCT 707 Query 1012 TGTGAGTCCATCGAAGGTAGATTCGTTCTACATTCCATTTTATGACCCCAACGAAA-AAG 1070 ||||||| | || ||||| || || ||| |||| || |||||||| || | | ||| ||| Sbjct 708 TGTGAGTGCGTCAAAGGTGGACTCTTTCAACATACCGTTTTATGATCCGAGC-AAAGAAG 766 Query 1071 AAGTAAAGGAGGTGGTACAGAAA-GAAGGTTCCTTTGAAATCAAAGACTTGGAGACACAT 1129 |||| | |||| || | | |||| ||||| || || || ||||| |||||||||| ||| Sbjct 767 AAGTGATGGAGATGATTC-GAAACGAAGGCTCATTCGAGATCAATGACTTGGAGATTCAT 825 Query 1130 GGATATGACCTTGGCC-ATTGTAACCAAGACGAG---T-CA----A-AG--AGAAGTA-- 1175 |||| ||| ||||||| | | ||||| |||||| | || | | | || || Sbjct 826 GGATTTGAGCTTGGCCTAA-GCAACCATGACGAGGACTACATGTTACATTCACAAATATC 884 Query 1176 -AAT-CAGGGAAAAATGAAGCCAATTACATCAGAGCGGTGAGTGAACCACTGCTCGTAGC 1233 || ||||| | | |||||||| | || |||||||| || ||| || |||| ||||| Sbjct 885 TAAAGCAGGGCAGAGGGAAGCCAACTGTATAAGAGCGGTCAGCGAATCAATGCTTGTAGC 944 Query 1234 TCACTTTGGAG-ATGCCATCACCAACATATTGTTTAACAAGTTTGCATGTCATGTGTCTC 1292 | |||| |||| | | ||| | ||| ||||||||| || ||||||| |||||||| | Sbjct 945 TGACTTCGGAGTA-GACATTATGGACACATTGTTTAAGAAATTTGCATACCATGTGTCAC 1003 Query 1293 AACATGCTAGTTGCAGGAACAAAACGACCGTCAG-CATAGTCGTTTCATTGACTAAGAAA 1351 |||||||||| |||| |||||||||||| || | | |||||||||| |||| || |||| Sbjct 1004 AACATGCTAGCTGCACGAACAAAACGACTGTTACTC-TAGTCGTTTCTTTGA-TACGAAA 1061 > AT3G11480.1 | Symbols: BSMT1, ATBSMT1 | S-adenosyl-L-methionine-dependent methyltransferases superfamily protein | chr3:3614437-3617314 FORWARD LENGTH=1424 Length=1424 Score = 686 bits (371), Expect = 0.0 Identities = 848/1076 (79%), Gaps = 41/1076 (4%) Strand=Plus/Plus Query 294 CTCTATGTATGAGTGGAGGAGATGGAT-ACAACAGTTACTCCACCAATTCTCTTCTTCAG 352 |||||||||||||||| |||||||| | ||||||||||||| ||||||||| |||||| Sbjct 190 CTCTATGTATGAGTGGTGGAGATGG-TGCCAACAGTTACTCCGCCAATTCTCGCCTTCAG 248 Query 353 AGAAGAGTGTTATCAAAGGCTAAACCGGTATTGGTTAAG-AACACA-AAGGAACTGATGA 410 | || ||| ||||||| ||| ||||| || |||| |||| |||||| || ||| |||||| Sbjct 249 AAAAAAGTTTTATCAATGGCCAAACCAGTCTTGG-TAAGAAACACAGAA-GAAATGATGA 306 Query 411 TAAACTTAAACTTTCCTACATACATTAAAGTAGCTGATTTGGGTTGTTCATCTGGACAAA 470 | |||||| |||||||||| ||||| ||||| ||||| ||||||||||| || ||||||| Sbjct 307 TGAACTTAGACTTTCCTACGTACATCAAAGTTGCTGAATTGGGTTGTTCTTCGGGACAAA 366 Query 471 ACACATTCTTGGCGATGTCTGAAATTATCAATA-CAGTCAATGTGTTATGTCAACAGAG- 528 || | || ||||| || | ||| || ||||| | || | |||||||| || ||||| | Sbjct 367 ACTCTTTTTTGGCTATCTTTGAGATCATCAACACCA-TTAATGTGTTGTGCCAACA-TGT 424 Query 529 GAACCAAAACCCACCAGAAATAGATTGTTGTCTAAATGATCTCCC-TAATAACGATTTTA 587 |||| ||||| ||||||| || |||||||||||||| |||||||| || || ||||| | Sbjct 425 GAACAAAAACTCACCAGAGATCGATTGTTGTCTAAACGATCTCCCGGAA-AATGATTTCA 483 Query 588 ACACAACGTTCAAATTCATACAATTCTTCAACGAGAAG---AACATCACGAGCAAAGAAT 644 |||| || || || ||| ||| ||||||||| || || | ||||| | |||| || Sbjct 484 ACACGACCTTTAAGTTCGTACCTTTCTTCAACAAGGAGCTCATGATCACAAACAAATCAT 543 Query 645 CATACTTT-GTCTCTGGAGTC-CCCGGTTCTTTCTACTCGAGGCTCTTCCCTCGGAGGAG 702 ||| ||| |||| ||||| | || ||||| ||||| || ||||||||| |||| | || Sbjct 544 CAT-GTTTCGTCTATGGAG-CTCCAGGTTCCTTCTATTCCAGGCTCTTCTCTCGCAATAG 601 Query 703 TCTTCATTTCGTACATTCCTCTTATGGTCTCCATTGGCTCTCTAAGGTTCCTGAAG-GGC 761 || ||||| ||||||||||||||| ||||||||||||||||||||||| | || | Sbjct 602 CCTCCATTTAATACATTCCTCTTATGCACTCCATTGGCTCTCTAAGGTTCCCG-AGAAAC 660 Query 762 TTAAGAAGAGTAAGATGAGTGTGTACATCACAAATTCAAGTCCTCTAAGTACATATAAGG 821 || |||| | |||| || | ||||||| |||| ||||||||||| |||| |||| |||| Sbjct 661 TTGAGAATAATAAGGGGAATCTGTACATAACAAGTTCAAGTCCTCAAAGTGCATACAAGG 720 Query 822 CTTACTTAAATCAATTCCAAACAGATTTCGCGACATTTCTTAA-ACTGCGTTCTGAAGAG 880 | ||||| ||||||||||||| ||| ||| | | |||| ||| | ||||||||||| Sbjct 721 CCTACTTGAATCAATTCCAAAAAGACTTCACCATGTTTC-TAAGGTTACGTTCTGAAGAA 779 Query 881 ATGGTTTCTAATGGACGCATGGTTCTCACTTTCATTGGTCGAAACACTATTGATAATCCA 940 || || ||||||||||| ||||||||||| ||||| || |||||||| || | ||||| Sbjct 780 ATTGTCTCTAATGGACGTATGGTTCTCACCTTCATCGGAAGAAACACTCTTAACGATCCA 839 Query 941 TTGCATAGAGATTGTTGTCACTTTTGGACATTATTATCCAGA-TCTCTCCGTGACCTAGT 999 ||| |||||||||||||||||||||||||||| |||| | | ||||||||||||||||| Sbjct 840 TTGTATAGAGATTGTTGTCACTTTTGGACATTGCTATCAA-ACTCTCTCCGTGACCTAGT 898 Query 1000 CGTCGAGGGTCTTGTGAGTCCA-TCGAAGGTAGATTCGTTCTACATTCCATTTTATGACC 1058 | | ||||||||||||||| | || ||| | || | ||| |||| || |||||||| | Sbjct 899 CTTTGAGGGTCTTGTGAGTG-AGTCAAAGCTGGACGCATTCAACATGCCGTTTTATGATC 957 Query 1059 CCAACGAAAAAGAAGT-AAAGGAGGTGGTACAGAAAGAAGGTTCCTTTGAAATCAAAGAC 1117 | |||| | ||||| | |||| || || |||| ||||| || || ||||||||||| || Sbjct 958 CGAACGTACAAGAACTCAAAGAAG-TGATACAAAAAGAGGGCTCTTTTGAAATCAATGAA 1016 Query 1118 TTGGAGACACATGGATATGACCTTGGCCATTG-TAACCAAGACGAGTCAAAGAGAAGTAA 1176 |||||| ||||||||| ||| ||||| || | || | |||| || | | |||| Sbjct 1017 TTGGAGTCACATGGATTTGATCTTGGTCACTACTA-CGAAGAAGA-TGACTTTGAAG--- 1071 Query 1177 ATCAGGGAAA-AATGAAGCCAATTACATCAGAGCGGTGAGTGAACCACTGCTCGTAGCTC 1235 |||| | |||||||| ||| ||| ||||| || ||||||||| ||||| | |||| Sbjct 1072 --CAGG-ACGCAATGAAGCTAATGGCATAAGAGCTGTTAGTGAACCAATGCTCATTGCTC 1128 Query 1236 ACTTTGGAGATGCCATCACCAACATATTGTTTAACAAGTTTGCATGTCATGTGTCTCAAC 1295 | |||||||| | || | | | | ||||| | |||| ||||| |||||| |||||| Sbjct 1129 ATTTTGGAGAAGAAATTATCGATACCTTGTTCGATAAGTATGCATACCATGTGACTCAAC 1188 Query 1296 ATGCTAGTTGCAGGAACAAAACGACCGTCAG-CATAGTCGTTTCATTGACTAAGAA 1350 |||| | ||||||||||||||||| ||||| | | |||||||| ||||||||||| Sbjct 1189 ATGCCAACTGCAGGAACAAAACGACTGTCAGTCTT-GTCGTTTCCTTGACTAAGAA 1243 Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 67921337385 Database: TAIR10_cdna_20110103_representative_gene_model_updated Posted date: Sep 25, 2014 6:13 PM Number of letters in database: 51,074,197 Number of sequences in database: 33,602 Matrix: blastn matrix 1 -2 Gap Penalties: Existence: 0, Extension: 2.5