BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg487282
Length=1228
Score E
Sequences producing significant alignments: (Bits) Value
AT5G04950.1 | Symbols: NAS1, ATNAS1 | nicotianamine synthase 1 ... 1866 0.0
AT5G56080.1 | Symbols: ATNAS2, NAS2 | nicotianamine synthase 2 ... 931 0.0
> AT5G04950.1 | Symbols: NAS1, ATNAS1 | nicotianamine synthase
1 | chr5:1457645-1458890 REVERSE LENGTH=1246
Length=1246
Score = 1866 bits (1010), Expect = 0.0
Identities = 1147/1212 (95%), Gaps = 13/1212 (1%)
Strand=Plus/Plus
Query 1 AAAAGATTATTGA-TACTTATCTTCTCTCCTGAAATAACCTCATAGTGTCGACATGGCTT 59
||||||||||| | ||||||||||||| |||||||||||||||||||||||| |||||||
Sbjct 1 AAAAGATTATT-ATTACTTATCTTCTCCCCTGAAATAACCTCATAGTGTCGATATGGCTT 59
Query 60 GCCAAAACAATCTCGTTGTGAAGCAAATCGTCGACTTATACGATCAAATCTCAAAGCTCG 119
||||||||||||||||||||||||||||| ||||||| ||||| |||||||||||||||
Sbjct 60 GCCAAAACAATCTCGTTGTGAAGCAAATCATCGACTTGTACGACCAAATCTCAAAGCTCA 119
Query 120 TGAGCTTAAAACCTTCCAAAAATGTCGACACTTTGTTCGGACAACTCGTGTCCACGTGCT 179
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 120 AGAGCTTAAAACCTTCCAAAAATGTCGACACTTTGTTCGGACAACTCGTGTCCACGTGCT 179
Query 180 TACCCACGGATACAAACATCGATGTCACAAATATGTGTGAAGAAGTCAAATACATGAGAT 239
|||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
Sbjct 180 TACCCACGGATACAAACATCGATGTCACAAATATGTGTGAAGAAGTCAAAGACATGAGAG 239
Query 240 CTAATCTCATCAAGCTTTGTGGTGAAGCCGAAGGTTATTTAGAGCAACACTTCTCCACAA 299
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 240 CTAATCTCATCAAGCTTTGTGGTGAAGCCGAAGGTTATTTAGAGCAACACTTCTCCACAA 299
Query 300 TTTTGGGATCTTTACAAGAAGACCAAAACCCACTTCACCATTTACACATCTTTCCTTACT 359
||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
Sbjct 300 TTTTGGGATCTTTACAAGAAGACCAAAACCCACTTGACCATTTACACATCTTTCCTTACT 359
Query 360 ACACCAACTACCTCAAGCTAGGCAAGCTCGAGTTCGATCTCCTGAGTCAACACTCAAGCC 419
|| ||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
Sbjct 360 ACTCCAACTACCTCAAGCTAGGCAAGCTCGAGTTCGATCTCCTGAGCCAACACTCAAGCC 419
Query 420 ATGTCCCCACCAAGATTGCCTTCGTGGGTTCGGGTCCGATGCCTCTCACATCCATCGTCT 479
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
Sbjct 420 ATGTCCCCACCAAGATTGCCTTCGTGGGTTCGGGTCCGATGCCTCTCACATCCATCGTAT 479
Query 480 TGGCCAAGTTTCACCTCCCGAACACGACCTTCCACAACTTCGACATCGACTCACACGCAA 539
||||||||||||||||||| |||||||| ||||||||||| |||||||||||||||||||
Sbjct 480 TGGCCAAGTTTCACCTCCCCAACACGACGTTCCACAACTTTGACATCGACTCACACGCAA 539
Query 540 ACACACTCGCTTCAAACCTCGTCTCTCGCGATCCGGACCTCTCAAAACGCATGATCTTCC 599
||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||
Sbjct 540 ACACACTCGCTTCAAACCTCGTCTCTCGCGACCCGGACCTCTCAAAACGCATGATCTTCC 599
Query 600 ACACAACGGACGTACTAAACGCTACCGAAGGCCTTGACCAATATGACGTCGTTTTCTTGG 659
|||||||||||||||||||||| ||||||||||||||||||||||||||||||||||| |
Sbjct 600 ACACAACGGACGTACTAAACGCAACCGAAGGCCTTGACCAATATGACGTCGTTTTCTTAG 659
Query 660 CGGCTCTTGTTGGGATGGACAAAGAGTCAAAGGTCAAAGCCATTGAGCACTTGGAGAAAC 719
|||| ||||| |||||||||||||||||||||||||||||||| ||||||||||||||||
Sbjct 660 CGGCGCTTGTAGGGATGGACAAAGAGTCAAAGGTCAAAGCCATCGAGCACTTGGAGAAAC 719
Query 720 ACATGGCTCCTGGAGCTGTCCTTATGCTAAGGAGTGCTCATGCTCTCAGAGCTTTCTTAT 779
||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 720 ACATGGCTCCTGGAGCTGTTCTTATGCTAAGGAGTGCTCATGCTCTCAGAGCTTTCTTAT 779
Query 780 ATCCCATCGTTGACTCTTCTGATCTCAAAGGCTTTCAGCTCTTGACCATCTATCATCCAA 839
|||| ||||||||||| |||||||||||||||||||| ||||||||||||||||||||||
Sbjct 780 ATCCAATCGTTGACTCGTCTGATCTCAAAGGCTTTCAACTCTTGACCATCTATCATCCAA 839
Query 840 CCGATGATGTGGTTAACTCGGTTGTGATCGCACGTAAGCTCGGTGGTTCGACCACGACCG 899
||||||| ||||||||||||||||||||||||||||||||||||||| |||||||| |||
Sbjct 840 CCGATGACGTGGTTAACTCGGTTGTGATCGCACGTAAGCTCGGTGGTCCGACCACGCCCG 899
Query 900 GGGTTAATGGTACTCGTGGCTGCATGTTTATGCCTTGTAACTGCTCCAAGATCCACGCGA 959
||||||||||||||||||| |||||||||||||||||||||||||||||||| |||||||
Sbjct 900 GGGTTAATGGTACTCGTGGATGCATGTTTATGCCTTGTAACTGCTCCAAGATTCACGCGA 959
Query 960 TCATGAACAACCGTGGTAAGAAGAATATAATCGAGGAGTTTAGTGCCATCGAGTAAACAA 1019
|||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 960 TCATGAACAACCGTGGTAAGAAGAATATGATCGAGGAGTTTAGTGCCATCGAGTAAACAA 1019
Query 1020 ATAATTAATTGTATTAACACATCTCTTTTCTTAAGATTGTGTTCATGTTTTTTGTCTTAT 1079
| ||||||| | |||||||||||||||||||||||||||| |||||||| |||||||
Sbjct 1020 ACAATTAATA--A--AACACATCTCTTTTCTTAAGATTGTGTTAATGTTTTT-GTCTTAT 1074
Query 1080 AATAACTGCTCTTTTCGTTTCATGTCTTTTGTGTGTTTGGGCTACTTTTGTTAAGTAT-A 1138
| |||||||||||||| ||||||||||| ||||||| ||| |||| || |||||| | |
Sbjct 1075 ATTAACTGCTCTTTTCATTTCATGTCTTGTGTGTGTGTGG-CTACGCTTATTAAGTGTTA 1133
Query 1139 AGTTTGTGAATTTAGAGTTCTTTGATCTC-GTTTTGTACGAA---GATACAGAACCCTTT 1194
||||||||||||| |||||||||||||| ||| || | ||| ||||| |||| |||
Sbjct 1134 AGTTTGTGAATTTTGAGTTCTTTGATCTTTGTTATGAATGAAAAAGATACCGAACATTTT 1193
Query 1195 GCTTGTTGTACT 1206
| ||||||||||
Sbjct 1194 GTTTGTTGTACT 1205
> AT5G56080.1 | Symbols: ATNAS2, NAS2 | nicotianamine synthase
2 | chr5:22711402-22712364 REVERSE LENGTH=963
Length=963
Score = 931 bits (504), Expect = 0.0
Identities = 828/981 (84%), Gaps = 36/981 (4%)
Strand=Plus/Plus
Query 53 ATGGCTTGCCAAAACAATCTCGTTGTGAAGCAAATCGTCGACTTATACGATCAAATCTCA 112
||||||||| ||||||| |||||||||||||||||| | ||||||||| | |||||||||
Sbjct 1 ATGGCTTGCGAAAACAACCTCGTTGTGAAGCAAATCATGGACTTATACAACCAAATCTCA 60
Query 113 AAGCTCGTGAGCTTAAAACCTTCCAAAAATGTCGACACTTTGTTCGGACAACTCGTGTCC 172
|| |||| |||||||||||| ||||| |||||||||||||||||| ||||||| ||||||
Sbjct 61 AACCTCGAGAGCTTAAAACCATCCAAGAATGTCGACACTTTGTTCAGACAACTTGTGTCC 120
Query 173 ACGTGCTTACCCACGGATACAAACATCGATGTCACA-A-ATATGTG-TGAAGAAGTCAAA 229
||||||||||| ||||| || ||||||||||||||| | ||| | |||| ||||||||
Sbjct 121 ACGTGCTTACCAACGGACACGAACATCGATGTCACAGAGATACACGATGAAAAAGTCAAA 180
Query 230 TACATGAGATCTAATCTCATCAAGCTTTGTGGTGAAGCCGAAGGTTATTTAGAGCAACAC 289
||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 181 GACATGAGATCTCATCTCATCAAGCTTTGTGGTGAAGCCGAAGGTTATTTAGAGCAACAC 240
Query 290 TTCTCCA-CAATTTTGGGATCTTTACAAGAAGACCAAAACCCACTTCACCATTTACACAT 348
|| || | |||| || || ||||| |||||| || ||| || |||||||||||||
Sbjct 241 TTTTC-AGCAATCTTAGGCTCTTTT---GAAGAC-AA--CCCTCTAAACCATTTACACAT 293
Query 349 CTTTCCTTACTACACCAACTACCTCAAGCTAGGCAAGCTCGAGTTCGATCTCCTGAG-TC 407
||| || || |||| |||||| ||||| |||||||| ||||| ||||||||||| | ||
Sbjct 294 CTTCCCCTATTACAACAACTATCTCAAACTAGGCAAACTCGAATTCGATCTCCT-ATCTC 352
Query 408 AACACTCAAGCCATGTCCCCACCAAGATT-GCCTTCGTGGGTTCGGGTCCGATGCCTCTC 466
||||| ||| ||||||||| ||||| | | ||||| | ||||| ||||||||||| ||
Sbjct 353 AACACACAACCCATGTCCCGACCAA-AGTCGCCTTTATTGGTTCCGGTCCGATGCCACTT 411
Query 467 ACATCCATCGTCTTGGCCAAGTTTCACCTCCCGAACACGACCTTCCACAACTTCGACATC 526
|| |||||||||||||||||||| |||||||| ||||| || ||||||||||||||||||
Sbjct 412 ACTTCCATCGTCTTGGCCAAGTTCCACCTCCCCAACACAACGTTCCACAACTTCGACATC 471
Query 527 GACTCACACGCAAACACACTCGCTTCAAACCTCGTCTCTCGCGATCCGGACCTCTCAAAA 586
||||||||||| ||||||||||||||||||||||| ||||| ||| | ||||| || |||
Sbjct 472 GACTCACACGCCAACACACTCGCTTCAAACCTCGTTTCTCGTGATTCTGACCTTTCCAAA 531
Query 587 CGCATGATCTTCCACACAACGGACGTACTAAACGCTACCGAAGGCCTT-GACCAATATGA 645
|||||||| ||||||||||| || ||| ||||||||| || || || |||||||| ||
Sbjct 532 CGCATGATTTTCCACACAACTGATGTATTAAACGCTAAGGAGGGG-TTAGACCAATACGA 590
Query 646 CGTCGTTTTCTTGGCGGCTCTTGTTGGGATGGACAAAGAGTCAAAGGTCAAAGCCATTGA 705
|| ||||||||||| ||||||||||||||||| |||||||||||||||||||| |||||
Sbjct 591 TGTTGTTTTCTTGGCAGCTCTTGTTGGGATGGATAAAGAGTCAAAGGTCAAAGCTATTGA 650
Query 706 GCACTTGGAGAAACACATGGCTCCTGGAGCTGTCCTTATGCTAAGGAGTGCTCATGCTCT 765
||| || ||||| || ||||||||||||||||| | |||||||| |||||||||| |||
Sbjct 651 GCATTTAGAGAAGCATATGGCTCCTGGAGCTGTGGTGATGCTAAGAAGTGCTCATGGTCT 710
Query 766 CAGAGCTTTCTTATATCCCATCGTTGACTCTTCTGATCTCAAAGGCTTTCAGCTCTTGAC 825
||||||||||| ||||| ||||||||||||| |||||| ||||| ||| || | || ||
Sbjct 711 TAGAGCTTTCTTGTATCCAATCGTTGACTCTTGTGATCTTAAAGGGTTTGAGGTGTTAAC 770
Query 826 CATCTATCATCCAACCGATGATGTGGTTAACTCGGTTGTGATCGCACGTAAGCTCGGTGG 885
||| |||||||| | || || |||||||| ||||| || |||||||||||||| |||||
Sbjct 771 CATTTATCATCCGTCTGACGACGTGGTTAATTCGGTGGTCATCGCACGTAAGCTTGGTGG 830
Query 886 TTCGACCACG-A-C-CG-GG--GTTA-AT-GGTACTCGTGGCTGCATGTTTATGCCTTGT 937
||| | | | | | || || | | || || || ||| || || |||||||||||
Sbjct 831 TTCAA--ATGGAGCTCGAGGCAGCCAGATCGG-ACG-GTG--TG--TGGTTATGCCTTGT 882
Query 938 AACTGCTCCAAGATCCACGCGATCATGAACAACCGTGGTA---AGAAGAATATAATCGAG 994
|| ||||| ||| ||||||||||| ||||||| ||||||| |||||||| | ||||||
Sbjct 883 AATTGCTCTAAGGTCCACGCGATCTTGAACAATCGTGGTATGGAGAAGAATTTGATCGAG 942
Query 995 GAGTTTAGTGCCATCGAGTAA 1015
|||| ||||||||||||||||
Sbjct 943 GAGTATAGTGCCATCGAGTAA 963
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 60341055090
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5