BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg487553
Length=3545
Score E
Sequences producing significant alignments: (Bits) Value
AT5G07280.1 | Symbols: EMS1, EXS | Leucine-rich repeat transmem... 5134 0.0
> AT5G07280.1 | Symbols: EMS1, EXS | Leucine-rich repeat transmembrane
protein kinase | chr5:2285040-2288686 FORWARD LENGTH=3647
Length=3647
Score = 5134 bits (2780), Expect = 0.0
Identities = 3159/3346 (94%), Gaps = 10/3346 (0%)
Strand=Plus/Plus
Query 205 ATTCCGAAGGAGATTTCAACTTTGAAGAATCTCAAAGAGCTTCGTCTAGCCGGAAATCAA 264
||||||||||| ||||| | ||||||||| || | ||||||| |||| ||||||||||||
Sbjct 289 ATTCCGAAGGAAATTTCGAGTTTGAAGAACCTTAGAGAGCTTTGTCTTGCCGGAAATCAA 348
Query 265 TTCTCCGGGAAGATTCCGTCTGAGATTTGGAAACTAAAACAGCTTCAAACCCTAGACCTC 324
|||||||||||||||||| ||||||||||||| |||||||| || |||||||| ||||||
Sbjct 349 TTCTCCGGGAAGATTCCGCCTGAGATTTGGAATCTAAAACATCTCCAAACCCTTGACCTC 408
Query 325 TCTGGAAACTCACTCACAGGACTGCTCCCGAGTCAACTCAGTGAGTTACATCAGCTTCTC 384
|| ||||| || ||||| ||||| |||||||| | |||||||||||||| ||||||||||
Sbjct 409 TCAGGAAATTCTCTCACCGGACTTCTCCCGAGGCTACTCAGTGAGTTACCTCAGCTTCTC 468
Query 385 TATCTTGACCTAAGTGACAACCACTTCTCCGGTTCACTTCCTCCGTCGTTCTTCCTCAGC 444
|| || ||||| |||||||||||||||||||||||||||||||| ||||||||| |||||
Sbjct 469 TACCTCGACCTTAGTGACAACCACTTCTCCGGTTCACTTCCTCCATCGTTCTTCATCAGC 528
Query 445 TTCCCTGCACTGTCTTCACTCGACGTCTCCAACAACTCTCTCTCCGGCGAAATCCCTCCA 504
|| |||||||||||||| || ||||||||||||||||||||||||||||||||||||||
Sbjct 529 TTACCTGCACTGTCTTCTCTTGACGTCTCCAACAACTCTCTCTCCGGCGAAATCCCTCCT 588
Query 505 GAAATCGGGAAATTGAGTAACCTCTCTGACCTTTACATGGGACTCAACTCATTTTCCGGT 564
|||||||||||| ||||||||||||| | ||||||||||||||||||||||||||||||
Sbjct 589 GAAATCGGGAAACTGAGTAACCTCTCCAATCTTTACATGGGACTCAACTCATTTTCCGGT 648
Query 565 CAAATCCCGCCGGAGGTTGGTAACATCTCGTTGCTGAAGAACTTTGGTGCACCTTCTTGC 624
||||| ||| ||||| |||||||||||||||||||||||||||||| |||||||||||||
Sbjct 649 CAAATTCCGTCGGAGATTGGTAACATCTCGTTGCTGAAGAACTTTGCTGCACCTTCTTGC 708
Query 625 TTCTTCAAAGGACCGTTACCAAAGGAGATTTCGAAGCTGAAACACTTAGCAAAGCTCGAC 684
|||||||| |||||||||||||||||||||||||| ||||||||||||||||||||||||
Sbjct 709 TTCTTCAATGGACCGTTACCAAAGGAGATTTCGAAACTGAAACACTTAGCAAAGCTCGAC 768
Query 685 CTTTCTTACAACCCACTCAAGTGTT-CAATCCCAAAATCGTTTGGGGAGTTGCAGAATCT 743
|||||||| || ||||||||||||| || |||| ||||||||||| |||||||| |||||
Sbjct 769 CTTTCTTATAATCCACTCAAGTGTTCCATTCCC-AAATCGTTTGGAGAGTTGCATAATCT 827
Query 744 GAGTATATTGAATCTTGTCTCCGCAGAGCTTATTGGATTGATCCCACCAGAGCTTGGTAA 803
||||||| | ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 828 GAGTATACTAAATCTTGTCTCCGCAGAGCTTATTGGATTGATCCCACCAGAGCTTGGTAA 887
Query 804 GTGTAAAAGCCTCAAGACACTGATGCTTTCTTTCAATTCTCTCTCTGGTTCTTTGCCATT 863
||||||||||||||| |||||||||| |||||||||||||||||||| ||||||||||
Sbjct 888 TTGTAAAAGCCTCAAGTCACTGATGCTGTCTTTCAATTCTCTCTCTGGACCTTTGCCATT 947
Query 864 GGAGCTTTCAGAGATTCCTCTGTTGACGTTTTCTGCTGAAAGGAATCAGCTTTCGGGTTC 923
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 948 GGAGCTTTCAGAGATTCCTCTGTTGACGTTTTCTGCTGAAAGGAATCAGCTTTCGGGTTC 1007
Query 924 ATTGCCTTCATGGATCGGCAAATGGAAGGTACTGGACTCACTTCTGTTAGCTAACAATCG 983
||||||||||||||| || |||||||||||||||||||||||||||||||||||||||||
Sbjct 1008 ATTGCCTTCATGGATGGGGAAATGGAAGGTACTGGACTCACTTCTGTTAGCTAACAATCG 1067
Query 984 GTTTTCAGGTGAAATCCCTCGTGAGATTGAGGATTGTCCAATGTTGAAGCATCTCAGTCT 1043
|||||||||||||||||||| ||||||||||||||||||||||||||| |||||||||||
Sbjct 1068 GTTTTCAGGTGAAATCCCTCATGAGATTGAGGATTGTCCAATGTTGAAACATCTCAGTCT 1127
Query 1044 AGCTAGCAACTTGTTAACTGGTTCGATTCCTAGGGAGCTTTGTGGTTCAGGTTCGTTGGA 1103
||||||||||||||||| ||||||||||||||||||||| ||||||||||||||||||||
Sbjct 1128 AGCTAGCAACTTGTTAAGTGGTTCGATTCCTAGGGAGCTCTGTGGTTCAGGTTCGTTGGA 1187
Query 1104 GGAGATTGATCTCTCTGGGAATTTGCTTTCGGGAACAATTGAGGAGGTGTTCAATGGTTG 1163
|| ||||||||||||||||||||||||||| ||||||||||||||||| || |||| ||
Sbjct 1188 GGCGATTGATCTCTCTGGGAATTTGCTTTCTGGAACAATTGAGGAGGTCTTTGATGGATG 1247
Query 1164 TTCGTCTCTTGTAGAGTTGGTT-CTTACCAATAATCAGATCAATGGCTCTATTCCTGAGG 1222
||| ||||||| |||||| ||| ||||| |||||||||||||||||||||||||||||||
Sbjct 1248 TTCCTCTCTTGGAGAGTT-GTTACTTACTAATAATCAGATCAATGGCTCTATTCCTGAGG 1306
Query 1223 ATCTTTCAAAGCTTCCATTGATGGCAGTTGACTTGGATTCCAACAACTTCACAGGAGAAA 1282
|||||| |||||||||||||||||| |||||||||||||||||||||||||||||||||
Sbjct 1307 ATCTTTGGAAGCTTCCATTGATGGCACTTGACTTGGATTCCAACAACTTCACAGGAGAAA 1366
Query 1283 TCCCAAAGAGTCTCTGGAAATCAACGAACTTGATGGAGTTCT-CAGCTTCTTATAATCGT 1341
|||||||||||||||||||||||||||||||||||||||| | |||||||||||||||||
Sbjct 1367 TCCCAAAGAGTCTCTGGAAATCAACGAACTTGATGGAGTT-TACAGCTTCTTATAATCGT 1425
Query 1342 CTAGAAGGTTATTTGCCTGCAGAGATTGGTAATGCAGCTTCACTGACGCGGCTGGTTCTC 1401
|||||||||||||||||||||||||||||||||||||||||||||| |||| ||||||||
Sbjct 1426 CTAGAAGGTTATTTGCCTGCAGAGATTGGTAATGCAGCTTCACTGAAGCGGTTGGTTCTC 1485
Query 1402 AGTGATAATCAGTTAAAAGGTGAAATCCCAAGAGAGATTGGAAAACTCACATCATTGTCT 1461
|||||||||||||||| |||||||||||||||||||||||||||||||||||| ||||||
Sbjct 1486 AGTGATAATCAGTTAACAGGTGAAATCCCAAGAGAGATTGGAAAACTCACATCTTTGTCT 1545
Query 1462 GTCTTGAATCTGAATTCAAACAAGCTTCAAGGGAAGATACCAAACGAGCTTGGTGACTGT 1521
||||||||||||||| |||||| | ||||||||||||||||| ||||| ||||| |||
Sbjct 1546 GTCTTGAATCTGAATGCAAACATGTTTCAAGGGAAGATACCAGTTGAGCTCGGTGATTGT 1605
Query 1522 ACTTGTTTGACTACATTGGATCTTGGCAACAACAATCTTCAAGGACAGATTCCAGACAGA 1581
|||| |||||||||||||||||| |||| ||||||||||||||||||||||||||||| |
Sbjct 1606 ACTTCTTTGACTACATTGGATCTAGGCAGCAACAATCTTCAAGGACAGATTCCAGACAAA 1665
Query 1582 ATCACTGGTCTTTCTCAGCTTCAATGCCTGGTTCTCTCTTACAACAATCTCTCAGGGTCA 1641
||||||| ||| |||||||||| ||||||||||||||||||||||||||||| ||||||
Sbjct 1666 ATCACTGCTCTCGCTCAGCTTCAGTGCCTGGTTCTCTCTTACAACAATCTCTCCGGGTCA 1725
Query 1642 ATTCCATCAAAACCCTCTGCTTATTTCCATCAGATTGACATGCCTGATCTTAGCTTTCTT 1701
|||||||||||||||||||||||||||||||||||||| ||||||||||| |||||||||
Sbjct 1726 ATTCCATCAAAACCCTCTGCTTATTTCCATCAGATTGAGATGCCTGATCTCAGCTTTCTT 1785
Query 1702 CAGCACCATGGGATATTTGATCTCTCCTACAATAGATTGTCTGGCTCTATACCAGAAGAA 1761
||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||
Sbjct 1786 CAGCACCATGGGATATTTGATCTCTCCTACAATAGATTGTCTGGCCCTATACCAGAAGAA 1845
Query 1762 CTTGGCGACTGTGTGGTATTGGTGGAGATTTTACTGAGCAACAACCATCTCTCTGGAGAG 1821
|||||||| ||| |||||||||| ||||||| ||||||||||||||||||||||||||||
Sbjct 1846 CTTGGCGAGTGTTTGGTATTGGTAGAGATTTCACTGAGCAACAACCATCTCTCTGGAGAG 1905
Query 1822 ATTCCAGCTTCACTCTCTCGTTTGACCAACCTCACAATCCTGGATCTATCTGGAAATGCA 1881
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1906 ATTCCAGCTTCACTCTCTCGTTTGACCAACCTCACAATCCTGGATCTATCTGGAAATGCA 1965
Query 1882 CTTACTGGATCTATTCCAAAGGAGATGGGCCACTCTCTTAAGTTGCAGGGATTAAACTTG 1941
|| ||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
Sbjct 1966 CTGACTGGATCTATTCCAAAGGAGATGGGCAACTCTCTTAAGTTGCAGGGATTAAACTTG 2025
Query 1942 GCGAATAATCAGCTCAATGGCTATATACCAGAAAGCTTTGGTC-TGTTAGATAGCTTGGT 2000
||||||||||||||||||||| |||| |||||||||||||| | |||||| |||||||||
Sbjct 2026 GCGAATAATCAGCTCAATGGCCATATTCCAGAAAGCTTTGG-CTTGTTAGGTAGCTTGGT 2084
Query 2001 GAAGCTAAACTTGACCAAGAACAAGCTGGATGGATCGGTTCCTGCTTCTCTTGGGAACTT 2060
||||||||||||||| ||||||||||| |||||| |||||||||||||||||||||||||
Sbjct 2085 GAAGCTAAACTTGACTAAGAACAAGCTTGATGGACCGGTTCCTGCTTCTCTTGGGAACTT 2144
Query 2061 GAAAGAGCTTACACACATGGACTTGAGCTTTAACAACCTAAGTGGCGAGCTTTCATCAGA 2120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2145 GAAAGAGCTTACACACATGGACTTGAGCTTTAACAACCTAAGTGGCGAGCTTTCATCAGA 2204
Query 2121 ACTGTCTACAATGGTCAAACTTGTAGGCCTCTACATTGAGCAGAATAAGTTCACCGGAGA 2180
|||||||||||||| |||||||| |||||||||||||||||||||||||||||||| ||
Sbjct 2205 ACTGTCTACAATGGAAAAACTTGTCGGCCTCTACATTGAGCAGAATAAGTTCACCGGGGA 2264
Query 2181 AATACCGTCAGAACTTGGTAATCTTACACAGCTGGAGTACCTTGATGTTTCTGAGAACTT 2240
|||||| |||||||||||||||||||| ||||||||||||||||||||||||||||||||
Sbjct 2265 AATACCTTCAGAACTTGGTAATCTTACGCAGCTGGAGTACCTTGATGTTTCTGAGAACTT 2324
Query 2241 GCTATCTGGAGAAATCCCAACAAAGATCTGTGGATTGCCCAATCTGGAGTTCTTGAATCT 2300
| |||||||||| || ||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2325 GTTATCTGGAGAGATACCAACAAAGATCTGTGGATTGCCCAATCTGGAGTTCTTGAATCT 2384
Query 2301 AGCCAAGAACAATCTGCGAGGAGAGGTGCCGAGTGATGGTGTTTGCCAGGATCCTTCCAA 2360
||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||
Sbjct 2385 AGCCAAGAACAATCTGCGAGGAGAGGTGCCGAGTGATGGTGTTTGTCAGGATCCTTCCAA 2444
Query 2361 GGCATTGCTTTCAGGGAACAAAGAGTTGTGTGGCAGAGTTATTGGATCAGATTGCAAGAT 2420
||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
Sbjct 2445 GGCATTGCTTTCAGGGAACAAAGAGTTGTGTGGCAGAGTGGTTGGATCAGATTGCAAGAT 2504
Query 2421 CGATGGAACTAAGTTGACTCA-TGCTTGGGGGATTGCAGGACTCATGCTTGGTTTTACGA 2479
||| ||||||||||||| | |||||||||||||||||||||||||||||| |||||||
Sbjct 2505 CGAGGGAACTAAGTTGA-GGAGTGCTTGGGGGATTGCAGGACTCATGCTTGGCTTTACGA 2563
Query 2480 TCATCGTCTTTGTGTTTGTATTCTCTCTGCGCAGATGGGTTATGACAAAGAGAGTGAAGC 2539
||||||| ||||||||||| ||||||||||||||||||| ||||||||||||||||||||
Sbjct 2564 TCATCGTTTTTGTGTTTGTTTTCTCTCTGCGCAGATGGGCTATGACAAAGAGAGTGAAGC 2623
Query 2540 AAAGAGATGATCCAGAGCGAATGGAGGAAAGCAGGTTAAAAGGGTTTGTTGATCAGAACC 2599
|||||||||||||||||||||||||||||||||| || ||||||||||||||||||||||
Sbjct 2624 AAAGAGATGATCCAGAGCGAATGGAGGAAAGCAGATTGAAAGGGTTTGTTGATCAGAACC 2683
Query 2600 TGTATTTCTTAAGTGGAAGCAGATCAAGAGAGCCATTAAGCATCAATATAGCAATGTTCG 2659
|||||||||||||||||||||||||||| ||||| |||||||||||||||||||||||||
Sbjct 2684 TGTATTTCTTAAGTGGAAGCAGATCAAGGGAGCCTTTAAGCATCAATATAGCAATGTTCG 2743
Query 2660 AGCAGCCGCTTTTGAAGGTGCGTTTAGGAGATATTGTCGAGGCTACAGATCATTTCTCTA 2719
||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2744 AGCAGCCGCTTCTGAAGGTGCGTTTAGGAGATATTGTCGAGGCTACAGATCATTTCTCTA 2803
Query 2720 AGAAGAACATTATAGGAGACGGTGGTTTTGGGACGGTTTACAAAGCTTGTTTACCAGGTG 2779
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2804 AGAAGAACATTATAGGAGACGGTGGTTTTGGGACGGTTTACAAAGCTTGTTTACCAGGTG 2863
Query 2780 GGAAAACAGTAGCGGTGAAGAAGCTAAGTGAAGCCAAAACTCAGGGAAACCGGGAATTCA 2839
||||||||||||||||||||||||||||||||||||||| ||||||||||| |||||||
Sbjct 2864 AGAAAACAGTAGCGGTGAAGAAGCTAAGTGAAGCCAAAACGCAGGGAAACCGAGAATTCA 2923
Query 2840 TGGCAGAGATGGAGACTCTAGGGAAAGTGAAGCATCCGAATCTTGTGTCTTTGCTTGGAT 2899
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2924 TGGCAGAGATGGAGACTCTAGGGAAAGTGAAGCATCCGAATCTTGTGTCTTTGCTTGGAT 2983
Query 2900 ATTGTTCTTTCAGTGAAGAGAAGCTTCTGGTATATGAGTATATGGTTAATGGAAGCTTAG 2959
|||| ||||||||||||||||||||||||||||||||||||||||| |||||||||||||
Sbjct 2984 ATTGCTCTTTCAGTGAAGAGAAGCTTCTGGTATATGAGTATATGGTAAATGGAAGCTTAG 3043
Query 2960 ATCACTGGTTAAGGAACCAAACTGGGATGCTAGAGGTTCTGGACTGGTCAAAACGCCTCA 3019
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3044 ATCACTGGTTAAGGAACCAAACTGGGATGCTAGAGGTTCTGGACTGGTCAAAACGCCTCA 3103
Query 3020 AAATCGCAGTTGGAGCTGCAAGAGGTCTTGCTTTCCTACATCACGGTTTCATCCCTCACA 3079
|||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 3104 AAATCGCAGTTGGAGCTGCAAGAGGTCTAGCTTTCCTACATCACGGTTTCATCCCTCACA 3163
Query 3080 TTATACATAGGGACATCAAAGCCAGCAACATTCTCTTAGATGGTGATTTTGAGCCTAAGG 3139
||||||| |||||||||||||| ||||||||||| ||||||||||||||||| || ||||
Sbjct 3164 TTATACACAGGGACATCAAAGCGAGCAACATTCTGTTAGATGGTGATTTTGAACCAAAGG 3223
Query 3140 TGGCGGATTTCGGGCTAGCAAGACTAATTAGTGCCTGTGAATCCCATGTTAGTACAGTCA 3199
|||||||||| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3224 TGGCGGATTTTGGGCTAGCAAGACTAATTAGTGCCTGTGAATCCCATGTTAGTACAGTCA 3283
Query 3200 TCGCAGGGACTTTCGGGTATATCCCACCGGAGTATGGTCAGAGCGCAAGAGCCACAACCA 3259
| ||||||||||||||||||||||||||||||||||||||||| || ||||||||||||
Sbjct 3284 TAGCAGGGACTTTCGGGTATATCCCACCGGAGTATGGTCAGAGTGCCCGAGCCACAACCA 3343
Query 3260 AAGGGGATGTCTACAGCTTTGGAGTGATTCTGTTAGAACTAGTCACAGGGAAAGAGCCGA 3319
|||| |||||||| |||||||| ||||| || || || || ||||||||||||||||| |
Sbjct 3344 AAGGAGATGTCTATAGCTTTGGCGTGATACTATTGGAGCTTGTCACAGGGAAAGAGCCAA 3403
Query 3320 CCGGTCCAGACTTCAAAGAGAGTGAAGGAGGAAACTTGGTTGGTTGGGTGACTCGGAAGA 3379
||||||||||||| || |||||||||||||| |||||||||||||||| || || |||||
Sbjct 3404 CCGGTCCAGACTTTAAGGAGAGTGAAGGAGGGAACTTGGTTGGTTGGGCGATTCAGAAGA 3463
Query 3380 TTAACCAAGGGAAAGCGGTGGATGTTCTTGACCCACTGCTTGTATCTGTGGCCTTGAAGA 3439
| |||||||||||||| ||||||||| |||| |||||||| ||||||||||| |||||||
Sbjct 3464 TCAACCAAGGGAAAGCTGTGGATGTTATTGATCCACTGCTCGTATCTGTGGCTTTGAAGA 3523
Query 3440 ACTCTCTGCTTCGTCTGCTTCAGATTGCTATGGTCTGCCTTGCTGAAACGCCAGCTAATC 3499
|||||| |||||||||||||||||| |||||| | || |||||||||||||||||||| |
Sbjct 3524 ACTCTCAGCTTCGTCTGCTTCAGATAGCTATGCTTTGTCTTGCTGAAACGCCAGCTAAGC 3583
Query 3500 GCCCAAACATGCTTGATGTGTTGAAGGCCCTCAAGGATATATGAGC 3545
|||| ||||||||||||||||||||||| || ||||| ||||||||
Sbjct 3584 GCCCTAACATGCTTGATGTGTTGAAGGCTCTTAAGGAGATATGAGC 3629
Score = 217 bits (117), Expect = 7e-55
Identities = 179/207 (86%), Gaps = 12/207 (6%)
Strand=Plus/Plus
Query 1 ATGACGTTTCTTCCCGCCTTGTTCCTGTGGTTTCTCTTCTTCTCTGTTTCAGA-CACCGC 59
||| |||||||| |||| ||||||| | |||||||||||||||| |||| || ||||
Sbjct 49 ATGGCGTTTCTTACCGCATTGTTCC--T-GTTTCTCTTCTTCTCTTTTTC-GAGTTCCGC 104
Query 60 CATTGTTGATCTAAGCTCAGAGACGAGTTCTCTGC-TCTCCTTCAAGCACTCACTGGAAA 118
|||||||||||| ||||| || || | |||||| | |||||||||||| |||||| ||||
Sbjct 105 CATTGTTGATCTTAGCTCGGAAACAACTTCTCT-CATCTCCTTCAAGCGCTCACTAGAAA 163
Query 119 ACCCATCTCGCCTTTCTTCATGGAACGTTTCGTCTTCCTCGTCGTCTCACTGCGATTGGG 178
||||||||| |||||||||||||||||||||||||||| | ||||||||||||||||||
Sbjct 164 ACCCATCTCTCCTTTCTTCATGGAACGTTTCGTCTTCC--G-CGTCTCACTGCGATTGGG 220
Query 179 TCGGCGTTACGTGTTTG-TTCGGACGA 204
| |||||||||||| || || ||||||
Sbjct 221 TTGGCGTTACGTGTCTGCTT-GGACGA 246
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 176487305474
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5