BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg489181
Length=617
Score E
Sequences producing significant alignments: (Bits) Value
AT5G23230.1 | Symbols: NIC2 | nicotinamidase 2 | chr5:7825882-7... 821 0.0
AT5G23220.1 | Symbols: NIC3 | nicotinamidase 3 | chr5:7819816-7... 802 0.0
AT5G23235.1 | Symbols: | pseudogene, antisense mRNA to genes A... 763 0.0
> AT5G23230.1 | Symbols: NIC2 | nicotinamidase 2 | chr5:7825882-7826611
REVERSE LENGTH=730
Length=730
Score = 821 bits (444), Expect = 0.0
Identities = 565/623 (91%), Gaps = 9/623 (1%)
Strand=Plus/Plus
Query 1 ATGGCTTCGTCATCAACGAGAAAGTACGAGACACGAAAGCGAGATCCAAATCCAAAAACC 60
|||||||| |||||| |||||| ||||||||||||||||||||| |||||||| |||| |
Sbjct 11 ATGGCTTCTTCATCATCGAGAACGTACGAGACACGAAAGCGAGAGCCAAATCCTAAAATC 70
Query 61 GCAGCTCTTCTCGTTATCGATATGCAGAATCACTTCTCCTCCATGGCCAAACCCATCCT- 119
|||||||||||||| |||||||||||||||||||||| ||| ||||| |||| |||||
Sbjct 71 GCAGCTCTTCTCGTCATCGATATGCAGAATCACTTCTACTCTATGGCTGAACCAATCCTC 130
Query 120 CAACAACGTTCTCACCACCATCGACATCTGCCGACGCGCCTCAATCCCCATATTTTTCAC 179
||| |||| |||||||||||||||||||||||||||||| ||||||||| |||| |||||
Sbjct 131 CAA-AACGCTCTCACCACCATCGACATCTGCCGACGCGCTTCAATCCCCGTATTCTTCAC 189
Query 180 GCGCCACAACCACAAATCCCCAACCGATCACGGCATGCTCGGCGAGTGGTGGAACGGCGA 239
||||||||||||||||||||||||||| |||||||||||||| |||||||||||||||||
Sbjct 190 GCGCCACAACCACAAATCCCCAACCGACCACGGCATGCTCGGAGAGTGGTGGAACGGCGA 249
Query 240 TCTCATCCTCGACGGAACCACCGATTCCGAAATCATCCCGGAGATCCA--GATA-CTCAC 296
||| ||||||||||||||||| |||||||||||||||||||| ||| | | | ||||
Sbjct 250 TCTAATCCTCGACGGAACCACTGATTCCGAAATCATCCCGGAAATCAATCGCCAGGTCAC 309
Query 297 CAAACCAGACGAGATGGTGGAGAAGAGCACGTACAGCGCGTTTAACAACACGCGCCTCCA 356
| ||||||||| || |||||||||||||||||||| |||||||||||||||| ||| ||
Sbjct 310 CGGACCAGACGAAATCGTGGAGAAGAGCACGTACAGTGCGTTTAACAACACGCACCTTCA 369
Query 357 TGAAAAGCTGGACAAGATCGGGGTTAAGGAAGTGATCGTGATTGGAGTGATGACGAACCT 416
|| ||||||||||||||||| || ||||| |||||||| || |||||||||||||||||
Sbjct 370 GGAGAAGCTGGACAAGATCGGAGTGAAGGAGGTGATCGTTATCGGAGTGATGACGAACCT 429
Query 417 GTGCTGTGAGACGACGGCGCGTGAAGCGTTTGTGAAA-GGGTTCAGGGtttttttCTCGA 475
|||||||||||||||||||||||||||||||| ||| ||||| ||||||||||||||||
Sbjct 430 ATGCTGTGAGACGACGGCGCGTGAAGCGTTTGT-AAAGGGGTTTAGGGTTTTTTTCTCGA 488
Query 476 CGGACGCGACTGCGACGGTTAATGAAGAGCTTCACGAGGCTACACTAATGAATCTCGCTT 535
||||||||||||||||||||||||||||||||||||||||||| |||||||||||||| |
Sbjct 489 CGGACGCGACTGCGACGGTTAATGAAGAGCTTCACGAGGCTACTCTAATGAATCTCGCGT 548
Query 536 ATGGCTTCGCTTATCTCGTCGACTGCGATAGTCTCCGGCGAGGTCTACTCAGTAATAAAT 595
||||||| |||||||||||||| |||||||| |||||||||||||||||||||| | |||
Sbjct 549 ATGGCTTTGCTTATCTCGTCGATTGCGATAGACTCCGGCGAGGTCTACTCAGTAGTTAAT 608
Query 596 AAATGAT-ACTTACACCTTTTGT 617
||| ||| ||| |||||||||||
Sbjct 609 AAAAGATGACT-ACACCTTTTGT 630
> AT5G23220.1 | Symbols: NIC3 | nicotinamidase 3 | chr5:7819816-7820676
REVERSE LENGTH=861
Length=861
Score = 802 bits (434), Expect = 0.0
Identities = 558/617 (90%), Gaps = 11/617 (2%)
Strand=Plus/Plus
Query 1 ATGGCTTCGTCATCAACGAGAAAGTACGAGACACGAAAGCGAGATCCAAATC-CAAAAAC 59
|||||||| ||||||||||||||||||||||||||||||||||||||||| | | ||||
Sbjct 13 ATGGCTTCCTCATCAACGAGAAAGTACGAGACACGAAAGCGAGATCCAAA-CTCTAAAAT 71
Query 60 CGCAGCTCTTCTCGTTATCGATATGCAGAATCACTTCTCCTCCATGGCCAAACCCATCCT 119
||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
Sbjct 72 CGCAGCTCTTCTCGTTATCGACATGCAGAATCACTTCTCCTCCATGGCCAAACCCATCCT 131
Query 120 CAACAACGTTCTCACCACCATCGACATCTGCCGACGCGCCTCAATCCCCATATT-TTTCA 178
||||||||||||||||||||||||||||||||||||||||||| ||||| |||| ||| |
Sbjct 132 CAACAACGTTCTCACCACCATCGACATCTGCCGACGCGCCTCAGTCCCCGTATTCTTT-A 190
Query 179 CGCGCCACAACCACAAATCCCCAACCGATCACGGCATGCTCGGCGAGTGGTGGAACGGCG 238
|||| ||||||||||||||||| ||||| ||||||||||||||||||||||| |||||||
Sbjct 191 CGCGTCACAACCACAAATCCCCGACCGACCACGGCATGCTCGGCGAGTGGTGTAACGGCG 250
Query 239 ATCTCATCCTCGACGGAACCACCGATTCCGAAATCATCCCGGAGATCCA-GAT-AC-TCA 295
|| | ||||| ||||||||||||||||| |||||||||| |||||| || | | | |
Sbjct 251 ATGTAATCCTTGACGGAACCACCGATTCTGAAATCATCCAGGAGATACAAGGCCAAGTAA 310
Query 296 CCAAACCAGACGAGATGGTGGAGAAGAGCACGTACAGCGCGTTTAACAACACGCGCCTCC 355
|| ||||||||||||||||||||||| ||||||||| ||||||||||| || |||||||
Sbjct 311 CCGGACCAGACGAGATGGTGGAGAAGAACACGTACAGTGCGTTTAACAAAACCCGCCTCC 370
Query 356 ATGAAAAGCTGGACAAGATCGGGGTTAAGGAAGTGATCGTGATTGGAGTGATGACGAACC 415
| ||||| ||||| |||||||| || ||||| ||||||||||| |||||||||||||||
Sbjct 371 AGGAAAACCTGGAAAAGATCGGAGTAAAGGAGGTGATCGTGATCGGAGTGATGACGAACT 430
Query 416 TGTGCTGTGAGACGACGGCGCGTGAAGCGTTTGTGAAAGGGTTC-AGGGtttttttCTCG 474
||||||||||||| |||||||||||||||||| | || ||||| |||||||||||||||
Sbjct 431 TGTGCTGTGAGACAACGGCGCGTGAAGCGTTTATTAA-GGGTTTTAGGGTTTTTTTCTCG 489
Query 475 ACGGACGCGACTGCGACGGTTAATGAAGAGCTTCACGAGGCTACACTAATGAATCTCGCT 534
|||||||||||||||||| ||||||| ||||||||||||||||| |||||||||||||||
Sbjct 490 ACGGACGCGACTGCGACGTTTAATGAGGAGCTTCACGAGGCTACGCTAATGAATCTCGCT 549
Query 535 TATGGCTTCGCTTATCTCGTCGACTGCGATAGTCTCCGGCGAGGTCTACTCAGTAA-TAA 593
| ||||||||||||||||||||| ||||||| ||||||||| |||||||| |||| |||
Sbjct 550 TTTGGCTTCGCTTATCTCGTCGATTGCGATAAACTCCGGCGAAGTCTACTCGGTAACTAA 609
Query 594 ATAAATGATACTTACAC 610
| |||||||| ||||||
Sbjct 610 A-AAATGATAATTACAC 625
> AT5G23235.1 | Symbols: | pseudogene, antisense mRNA to genes
At5g23230 and At5g23240, blastp match of 34% identity and 7.4e-17
P-value to OMNI|TM0133 isochorismatase-related protein
{Thermotoga maritima MSB8} | chr5:7826028-7828724 FORWARD
LENGTH=2697
Length=2697
Score = 763 bits (413), Expect = 0.0
Identities = 523/576 (91%), Gaps = 7/576 (1%)
Strand=Plus/Minus
Query 1 ATGGCTTCGTCATCAACGAGAAAGTACGAGACACGAAAGCGAGATCCAAATCCAAAAACC 60
|||||||| |||||| |||||| ||||||||||||||||||||| |||||||| |||| |
Sbjct 574 ATGGCTTCTTCATCATCGAGAACGTACGAGACACGAAAGCGAGAGCCAAATCCTAAAATC 515
Query 61 GCAGCTCTTCTCGTTATCGATATGCAGAATCACTTCTCCTCCATGGCCAAACCCATCCT- 119
|||||||||||||| |||||||||||||||||||||| ||| ||||| |||| |||||
Sbjct 514 GCAGCTCTTCTCGTCATCGATATGCAGAATCACTTCTACTCTATGGCTGAACCAATCCTC 455
Query 120 CAACAACGTTCTCACCACCATCGACATCTGCCGACGCGCCTCAATCCCCATATTTTTCAC 179
||| |||| |||||||||||||||||||||||||||||| ||||||||| |||| |||||
Sbjct 454 CAA-AACGCTCTCACCACCATCGACATCTGCCGACGCGCTTCAATCCCCGTATTCTTCAC 396
Query 180 GCGCCACAACCACAAATCCCCAACCGATCACGGCATGCTCGGCGAGTGGTGGAACGGCGA 239
||||||||||||||||||||||||||| |||||||||||||| |||||||||||||||||
Sbjct 395 GCGCCACAACCACAAATCCCCAACCGACCACGGCATGCTCGGAGAGTGGTGGAACGGCGA 336
Query 240 TCTCATCCTCGACGGAACCACCGATTCCGAAATCATCCCGGAGATCCA--GATA-CTCAC 296
||| ||||||||||||||||| |||||||||||||||||||| ||| | | | ||||
Sbjct 335 TCTAATCCTCGACGGAACCACTGATTCCGAAATCATCCCGGAAATCAATCGCCAGGTCAC 276
Query 297 CAAACCAGACGAGATGGTGGAGAAGAGCACGTACAGCGCGTTTAACAACACGCGCCTCCA 356
| ||||||||| || |||||||||||||||||||| |||||||||||||||| ||| ||
Sbjct 275 CGGACCAGACGAAATCGTGGAGAAGAGCACGTACAGTGCGTTTAACAACACGCACCTTCA 216
Query 357 TGAAAAGCTGGACAAGATCGGGGTTAAGGAAGTGATCGTGATTGGAGTGATGACGAACCT 416
|| ||||||||||||||||| || ||||| |||||||| || |||||||||||||||||
Sbjct 215 GGAGAAGCTGGACAAGATCGGAGTGAAGGAGGTGATCGTTATCGGAGTGATGACGAACCT 156
Query 417 GTGCTGTGAGACGACGGCGCGTGAAGCGTTTGTGAAA-GGGTTCAGGGtttttttCTCGA 475
|||||||||||||||||||||||||||||||| ||| ||||| ||||||||||||||||
Sbjct 155 ATGCTGTGAGACGACGGCGCGTGAAGCGTTTGT-AAAGGGGTTTAGGGTTTTTTTCTCGA 97
Query 476 CGGACGCGACTGCGACGGTTAATGAAGAGCTTCACGAGGCTACACTAATGAATCTCGCTT 535
||||||||||||||||||||||||||||||||||||||||||| |||||||||||||| |
Sbjct 96 CGGACGCGACTGCGACGGTTAATGAAGAGCTTCACGAGGCTACTCTAATGAATCTCGCGT 37
Query 536 ATGGCTTCGCTTATCTCGTCGACTGCGATAGTCTCC 571
||||||| |||||||||||||| |||||||| ||||
Sbjct 36 ATGGCTTTGCTTATCTCGTCGATTGCGATAGACTCC 1
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 29738615024
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5