BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg489509
Length=1920
Score E
Sequences producing significant alignments: (Bits) Value
AT5G26780.3 | Symbols: SHM2 | serine hydroxymethyltransferase 2... 1838 0.0
AT4G37930.1 | Symbols: SHM1, STM, SHMT1 | serine transhydroxyme... 876 0.0
> AT5G26780.3 | Symbols: SHM2 | serine hydroxymethyltransferase
2 | chr5:9418195-9422091 FORWARD LENGTH=2072
Length=2072
Score = 1838 bits (995), Expect = 0.0
Identities = 1090/1137 (96%), Gaps = 1/1137 (0%)
Strand=Plus/Plus
Query 38 AGAG-AAGAAGAAAAATGGCGTTGGCTCTTCGTAGACTCTCGTCTTCCGTTAAGAAACCG 96
|||| || || |||||||||||||||||||||||||||||| ||||||||||||||||||
Sbjct 90 AGAGAAAAAAAAAAAATGGCGTTGGCTCTTCGTAGACTCTCCTCTTCCGTTAAGAAACCG 149
Query 97 ATCTCGCTTCTTTCTTCTAATGGCGATTCTCTCCGTTTCATGTCGTCTTTATCATCCTCA 156
||||||||||| || |||||||||| ||| |||||||||||||||||||||||| || ||
Sbjct 150 ATCTCGCTTCTCTCCTCTAATGGCGGTTCACTCCGTTTCATGTCGTCTTTATCAACCGCA 209
Query 157 GCTATGGCGGAGTCCGAGAAATCTCGTTCCAGTTGGATTAAGCAATTGAATGGATCTCTA 216
||||||||||| || |||||||||||||| |||||||||||||||||||||| |||||||
Sbjct 210 GCTATGGCGGAATCGGAGAAATCTCGTTCTAGTTGGATTAAGCAATTGAATGCATCTCTA 269
Query 217 GAAGAAATCGATCCCGAAGTTGCGGATATTATCGAATTAGAGAAAGCTAGGCAATGGAAG 276
|| ||||||||||| ||||||||||||||||||||||||||||| ||||| |||||||||
Sbjct 270 GATGAAATCGATCCTGAAGTTGCGGATATTATCGAATTAGAGAAGGCTAGACAATGGAAG 329
Query 277 GGATTTGAACTTATACCTTCAGAGAATTTCACCTCTCTCTCGGTTATGCAAGCGGTTGGT 336
||||||||||||||||| |||||||||||||| ||||| || || |||||||| ||||||
Sbjct 330 GGATTTGAACTTATACCGTCAGAGAATTTCACTTCTCTATCTGTGATGCAAGCCGTTGGT 389
Query 337 TCGGTTATGACTAATAAGTATAGTGAAGGATATCCTGGTGCTAGATACTATGGAGGAAAT 396
|| ||||||||||||||||||||||||||||| ||||| |||||||||||||||||||||
Sbjct 390 TCTGTTATGACTAATAAGTATAGTGAAGGATACCCTGGAGCTAGATACTATGGAGGAAAT 449
Query 397 GAGTACATTGACATGGCTGAGTCATTATGTCAAAAACGCGCATTAGAAGCTTTTCAGTTA 456
||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
Sbjct 450 GAGTACATTGACATGGCTGAGACATTATGTCAAAAACGCGCATTAGAAGCTTTTCAGTTA 509
Query 457 GATCCTTCGAAATGGGGAGTCAATGTGCAGTCTTTATCAGGATCACCAGCTAACTTCCAA 516
|||||||| |||||||||||||||||||||||||||||||||||||||||||| ||||||
Sbjct 510 GATCCTTCTAAATGGGGAGTCAATGTGCAGTCTTTATCAGGATCACCAGCTAATTTCCAA 569
Query 517 GTTTACACTGCATTGTTAAAGCCTCATGAAAGAATCATGGCACTTGATTTACCTCATGGT 576
||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
Sbjct 570 GTTTACACTGCATTGTTGAAGCCTCATGAAAGAATCATGGCACTTGATTTACCTCATGGT 629
Query 577 GGACATCTTTCTCATGGTTATCAGACTGACACGAAGAAAATATCGGCTGTATCCATCTTT 636
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 630 GGACATCTTTCTCATGGTTATCAGACTGACACGAAGAAAATATCGGCTGTATCCATCTTT 689
Query 637 TTTGAGACAATGCCATACAGATTGGATGAGAACACTGGTTACATTGATTACGATCAGCTA 696
|||||||||||||||||||||||||||||||||||||||||||||||||| || ||||||
Sbjct 690 TTTGAGACAATGCCATACAGATTGGATGAGAACACTGGTTACATTGATTATGACCAGCTA 749
Query 697 GAAAAAAGTGCGGTGCTTTTCAGACCCAAACTTATTGTTGCGGGTGCAAGTGCTTATGCT 756
||||| ||||| |||||||||||||| |||||||||||||||||||||||||||||||||
Sbjct 750 GAAAAGAGTGCCGTGCTTTTCAGACCGAAACTTATTGTTGCGGGTGCAAGTGCTTATGCT 809
Query 757 CGTCTATATGATTATGCACGTATTCGTAAGGTTTGTGACAAGCAAAAGGCAGTTATGCTG 816
||||||||||| |||||||||||||||||||| ||| |||||||||||||||||||| ||
Sbjct 810 CGTCTATATGACTATGCACGTATTCGTAAGGTCTGTAACAAGCAAAAGGCAGTTATGTTG 869
Query 817 GCTGATATGGCTCATATTAGTGGGTTGGTTGCTGCTGGCGTTATTCCTTCTCCTTTTGAG 876
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 870 GCTGATATGGCTCATATTAGTGGGTTGGTTGCTGCTGGCGTTATTCCTTCTCCTTTTGAG 929
Query 877 TATGCAGATGTTGTTACAACTACAACTCACAAATCGCTTCGCGGTCCTAGAGGGGCTATG 936
||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
Sbjct 930 TATGCAGATGTTGTTACGACTACAACTCACAAATCGCTTCGCGGTCCTAGAGGGGCTATG 989
Query 937 ATATTCTTTAGGAAGGGGTTAAAAGAGATTAACAAACAAGGGAAAGAGGTCATGTATGAC 996
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 990 ATATTCTTTAGGAAGGGGTTAAAAGAGATTAACAAACAAGGGAAAGAGGTCATGTATGAC 1049
Query 997 TATGAGGACAGAATTAACCAAGCTGTTTTTCCTGGACTGCAAGGTGGTCCACATAATCAC 1056
||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
Sbjct 1050 TATGAGGACAGAATTAATCAAGCTGTTTTTCCTGGACTGCAAGGTGGTCCACATAATCAC 1109
Query 1057 ACTATAACGGGTCTAGCAGTTGCTCTGAAGCAGGCGAGAACACCTGAGTATAAGGCCTAC 1116
||||||||||||||||||||||||||||||||||| ||||| |||||||| |||||||||
Sbjct 1110 ACTATAACGGGTCTAGCAGTTGCTCTGAAGCAGGCAAGAACTCCTGAGTACAAGGCCTAC 1169
Query 1117 CAGGATCAAGTTCTCCGTAATTGCTCAAAGTTTGCTGAGCTGGGTATAAGACCTACT 1173
||||||||||||||||||||||||||||||||||||||| ||| |||||||||||||
Sbjct 1170 CAGGATCAAGTTCTCCGTAATTGCTCAAAGTTTGCTGAGTTGGATATAAGACCTACT 1226
Score = 955 bits (517), Expect = 0.0
Identities = 634/687 (92%), Gaps = 21/687 (3%)
Strand=Plus/Plus
Query 1175 CTTTGCTTGCGAAAGGATATGATTTAGTGTCTGGAGGCACTGAGAATCATTTAGTTTTGG 1234
||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
Sbjct 1258 CTTTGCTTGCGAAAGGATATGATTTAGTGTCTGGAGGCACTGATAATCATTTAGTTTTGG 1317
Query 1235 TGAATCTGAAAAACAAGGGAATAGATGGATCAAGAGTGGAAAAAGTATTAGAATCAGTAC 1294
|||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
Sbjct 1318 TGAATCTGAAAAACAAGGGAATAGATGGATCAAGAGTGGAAAAAGTATTAGAATTAGTAC 1377
Query 1295 ATATTGCAGCAAACAAGAATACTGTTCCTGGTGATGTTTCTGCCATGGTTCCAGGTGGCA 1354
|||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
Sbjct 1378 ATATTGCAGCAAACAAGAATACTGTTCCTGGTGATGTTTCTGCCATGGTTCCCGGTGGCA 1437
Query 1355 TCCGTATGGGAACACCAGCCCTCACATCAAGAGGATTCATTGAGGAAGATTTTGCAAAAG 1414
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1438 TCCGTATGGGAACACCAGCCCTCACATCAAGAGGATTCATTGAGGAAGATTTTGCAAAAG 1497
Query 1415 TGGCGGAGTATTTTGATTTAGCCGTCAAGATAGCCTTAAAGATCAAGGCAGAGTCTCAAG 1474
|||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
Sbjct 1498 TGGCGGAGTATTTTGATTTAGCCGTCAAGATAGCCTTAAAGATCAAAGCAGAGTCTCAAG 1557
Query 1475 GAACAAAGTTGAAGGACTTTGTAGCAACAATGCAATCAAATGAGAAATTACAGTCAGAGA 1534
||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1558 GAACAAAGTTGAAAGACTTTGTAGCAACAATGCAATCAAATGAGAAATTACAGTCAGAGA 1617
Query 1535 TGGCAAAGTTGCGTGAGATGGTAGAAGAATATGCGAAGCAATTCCCAACAATTGGGTTCG 1594
|| ||||| | ||||||||||||||||||||||| |||||||||||||| ||||||||||
Sbjct 1618 TGTCAAAGCTCCGTGAGATGGTAGAAGAATATGCTAAGCAATTCCCAACGATTGGGTTCG 1677
Query 1595 AGAAAGAGACGATGAGATACAAGGAGTAGTTT-CAATCAAATGGATTGACACGTTTCCAC 1653
|||||||||||||||||||||| ||||||||| || || |||||||||||||||||||
Sbjct 1678 AGAAAGAGACGATGAGATACAAAGAGTAGTTTTCA-TCGTGTGGATTGACACGTTTCCAC 1736
Query 1654 ATCTCCGAGATGCTTTAGGTACCTAAAACACTTAGTTATACGTAAGCTTATTCTCTGAAA 1713
||||| |||||||||||||||||||||||||||| |||||| ||||||| |||| |||||
Sbjct 1737 ATCTCTGAGATGCTTTAGGTACCTAAAACACTTAATTATACATAAGCTTCTTCTATGAAA 1796
Query 1714 TATTT-G-AGGGAACTCATTATGGAGCGACTATGCAACTTTTTGCAGTGATCTTGGACCA 1771
||||| | ||||| |||||||||| |||| | |||||| |||||||||||| |||||||
Sbjct 1797 TATTTTGGAGGGA-CTCATTATGGGGCGATTGTGCAACCGTTTGCAGTGATCATGGACCA 1855
Query 1772 GAGGCG-AA--G-TA-TCT-------CG--GAAACTAAAGTTGAACTCGAAAAATGGCAC 1817
|||||| || | | ||| || ||||||||||||||||| |||||| ||||
Sbjct 1856 GAGGCGCAACCGATGGTCTGAAGTATCGCTGAAACTAAAGTTGAACTAAAAAAATCGCAC 1915
Query 1818 TCCAGTGTTGTCT-TTATC-TTTGCTT 1842
|||| |||||||| ||| | ||| |||
Sbjct 1916 TCCAATGTTGTCTCTTACCCTTTCCTT 1942
Score = 76.8 bits (41), Expect = 7e-13
Identities = 44/45 (98%), Gaps = 1/45 (2%)
Strand=Plus/Plus
Query 1 AACCGCCTTTTATATAATCGCACTCACTCAGAGAAACAGAGAAGA 45
||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 38 AACCG-CTTTTATATAATCGCACTCACTCAGAGAAACAGAGAAGA 81
> AT4G37930.1 | Symbols: SHM1, STM, SHMT1 | serine transhydroxymethyltransferase
1 | chr4:17831731-17834927 REVERSE LENGTH=1899
Length=1899
Score = 876 bits (474), Expect = 0.0
Identities = 1093/1386 (79%), Gaps = 66/1386 (5%)
Strand=Plus/Plus
Query 256 GAGAAAGCTAGGCAATGGAAGGGATTTGAACTTATACCTTCAGAGAATTTCACCTCTCTC 315
||||||||||| |||||||||||| |||||||||| || || ||||| ||||| ||| |
Sbjct 390 GAGAAAGCTAGACAATGGAAGGGACTTGAACTTATTCCATCTGAGAACTTCACATCTGTG 449
Query 316 TCGGTTATGCAAGCGGTTGGTTCGGTTATGACTAATAAGTATAGTGAAGGATATCCTGGT 375
||||| |||||||| ||||| || || |||||||| || || |||||||| |||||||||
Sbjct 450 TCGGTGATGCAAGCTGTTGGGTCTGTCATGACTAACAAATACAGTGAAGGCTATCCTGGT 509
Query 376 GCTAGATACTATGGAGGAAATGAGTACATTGACATGGCTGAGTCATTATGTCAAAAACGC 435
|| ||||||||||||||||||||||| || |||||||| || | ||||| || || |||
Sbjct 510 GCCAGATACTATGGAGGAAATGAGTATATAGACATGGCAGAAACCTTATGCCAGAAGCGC 569
Query 436 GCAT-TAGAAGCTTTTCAGTTAGATCCTTCG-AAA-TGGGGAGTCAATGTGCAGTCTTTA 492
|| | | |||||||| | ||||||||| | | ||| |||||||| ||||| || ||||
Sbjct 570 GC-TCTTGAAGCTTTCCGGTTAGATCC-T-GAAAAGTGGGGAGTGAATGTTCAACCTTTG 626
Query 493 TCAGGATCACCAGCTAACTTCCAAGTTTACACTGCATTGTTAAAGCCTCATGAAAGAATC 552
|| ||||| || || |||||||| || |||||||||||||||||||||||||||||||||
Sbjct 627 TCTGGATCTCCTGCCAACTTCCATGTGTACACTGCATTGTTAAAGCCTCATGAAAGAATC 686
Query 553 ATGGCACTTGAT-TTACCTCATGGTGGACATCTTTCTCATGGTTATCAGACTGACACGAA 611
|||||||||||| || ||||||||||| ||||||||||||||||||||||||||||| ||
Sbjct 687 ATGGCACTTGATCTT-CCTCATGGTGGTCATCTTTCTCATGGTTATCAGACTGACACCAA 745
Query 612 GAAAATATCGGCTGTATCCATCTTTTTTGAGACAATGCCATACAGATTGGATGAGAACAC 671
||| ||||| ||||| || ||||| ||||| |||||||| || |||||||| |||| |||
Sbjct 746 GAAGATATCAGCTGTGTCTATCTTCTTTGAAACAATGCCCTATAGATTGGACGAGAGCAC 805
Query 672 TGGTTACATTGATTACGATCAGCTAGAAAAAAGTGCGG-TGCTTTTCAGACCCAAACTT- 729
||| ||||| || ||||||||| | || |||||||| | |||||||| || ||| ||
Sbjct 806 TGGCTACATCGACTACGATCAGATGGAGAAAAGTGCTACT-CTTTTCAGGCCAAAA-TTG 863
Query 730 ATTGTTGCGGGTGCAAGTGCTTATGCTCG-TCTATATGATTATGCACGTATTC-GTAAGG 787
|||||||| |||||||||||||||||| | | | ||||| ||||| || || | | ||||
Sbjct 864 ATTGTTGCTGGTGCAAGTGCTTATGCTAGAT-TGTATGACTATGCCCGCAT-CAGAAAGG 921
Query 788 TTTGTGACAAGCAAAAGGCAGTTATGCTGGCTGATATGGCTCATATTAGTGGGTTGGTTG 847
| ||| |||||||||| || || ||||| || |||||||| || || ||||| |||||||
Sbjct 922 TCTGTAACAAGCAAAAAGCTGTAATGCTAGCAGATATGGCACACATCAGTGGTTTGGTTG 981
Query 848 CTGCTGGCGTTATTCCTTCTCCTTTTGAGTATGCAGATGTTGTTACAACTACAACTCACA 907
||||| || || ||||| || || || ||||| |||||||| || || ||||||||||
Sbjct 982 CTGCTAATGTAATCCCTTCACCGTTCGACTATGCTGATGTTGTAACCACCACAACTCACA 1041
Query 908 AATCGCTTCGCGGTCCTAGAGGGGCTATGATATTCTTTAGGAAGGG-GTTAAAAGAGATT 966
| || ||||| || || | || || ||||| ||||| || ||||| ||||| || |||
Sbjct 1042 AGTCACTTCGTGGACCCCGTGGAGCCATGATTTTCTTCAGAAAGGGTGTTAAG-GAAATT 1100
Query 967 AACAAACAAGGGAAAGAGGTCATGTATGACTATGAGGACA-GAATTAACCAAGCTGTTTT 1025
||||| |||||||||||||| ||||||| | ||| |||| || | ||||||||||| ||
Sbjct 1101 AACAAGCAAGGGAAAGAGGTTTTGTATGATTTTGAAGACAAGA-TCAACCAAGCTGTCTT 1159
Query 1026 TCCTGGACTGCAAGGTGGTCCACATAATCACACTATAACGGGTCTAGCAGTTGCTCTGAA 1085
||||| || |||||||||||||| || |||||||| || || ||||| |||||| ||||
Sbjct 1160 CCCTGGTCTTCAAGGTGGTCCACACAACCACACTATCACAGGACTAGCTGTTGCTTTGAA 1219
Query 1086 GCAGGCGAGAACACCTGAGTATAAGGCCTACCAGGATCAAGTTCTCCGTAATTGCT-CAA 1144
||||| | || | ||||| || || ||||| || ||||| || |||| | | |||
Sbjct 1220 ACAGGCAACTACTTCAGAGTACAAAGCATACCAAGAACAAGTCCTGAGTAACAG-TGCAA 1278
Query 1145 AGTTTGCTGAGCTGGGTATAAGACCTACTTCTTTGCTTGCGAAAGGATATGAT-TTAGTG 1203
|||||||| | | ||| | || | | | || ||||||||| || ||
Sbjct 1279 AGTTTGCT---C-----A---GAC-T-CTAAT--G---GAGAGAGGATATGAACTT-GTT 1319
Query 1204 TCTGGAGGCACTGAGAATCATTTAGTTTTGGTGAATCTGAAAAAC-AAGGGAATAGATGG 1262
||||| || ||||| || ||| | ||| | |||||||| ||| | |||||||| |||||
Sbjct 1320 TCTGGTGGAACTGACAACCATCTGGTTCTAGTGAATCT-AAAGCCCAAGGGAATTGATGG 1378
Query 1263 ATCAAGAGTGGAAAAAGTATTAGAATCAGTACATATTGCAGCAAACAAGAATACTGTTCC 1322
||| ||||| || ||||| || ||| | || || |||||| | ||||| || ||||||||
Sbjct 1379 ATCTAGAGTTGAGAAAGTGTTGGAAGCTGTTCACATTGCATCCAACAAAAACACTGTTCC 1438
Query 1323 TGGTGATGTTTCTGCCATGGTTCCAGGTGGCATCCGTATGGGAACACCAGCCCTCACATC 1382
||| |||||||||||||||||||| ||||| ||| | ||||| || || || ||||| ||
Sbjct 1439 TGGAGATGTTTCTGCCATGGTTCCTGGTGGAATCAGAATGGGTACTCCTGCTCTCACTTC 1498
Query 1383 AAGAGGATTCATTGAGGAAGATTTTGCAAAAGTGGCGGAGTATTTTGATTTAGCCGTCA- 1441
||||| || |||||||||| ||||| ||||| || || || || || ||| || |
Sbjct 1499 CAGAGGCTTTGTTGAGGAAGACTTTGCCAAAGTAGCTGAATACTTCGACAAAGCTGTGAC 1558
Query 1442 AGATAGC-CTTAAAGATCAAGGCAGA-GTCTCAAGGAACAAAGTTGAAGGACTTT-GTAG 1498
| ||||| || |||| |||| | || | |||||||||| ||||||||||| ||| || |
Sbjct 1559 A-ATAGCTCTCAAAG-TCAAATCTGAAG-CTCAAGGAACCAAGTTGAAGGA-TTTCGT-G 1613
Query 1499 -CAACAATGCAATCAAATGAGAA--ATTACAGTCAGAGATGGC-AAAGTTGCGTGA-GAT 1553
|| ||||| |||| | || || || || ||||| || ||| | ||| | ||
Sbjct 1614 TCAGCAATGGAATCCTCTTC-AACCATC-CAATCCGAGATTGCGAAACT-GCGCCATGAA 1670
Query 1554 GGTAGAAGAATAT-GCGAAGCAATTCCCAACAATTGGGTTCGAGAAAGAGACGATGA-GA 1611
| | || |||| | || ||||| ||||||||||||||||| |||||||| || |||| |
Sbjct 1671 G-TCGAGGAAT-TCGCTAAGCAGTTCCCAACAATTGGGTTTGAGAAAGAAACCATGAAG- 1727
Query 1612 TACAAG 1617
||||||
Sbjct 1728 TACAAG 1733
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 95079832230
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5