BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg489585
Length=1982
Score E
Sequences producing significant alignments: (Bits) Value
AT5G27410.1 | Symbols: | D-aminoacid aminotransferase-like PLP... 2964 0.0
AT3G05190.1 | Symbols: | D-aminoacid aminotransferase-like PLP... 1827 0.0
> AT5G27410.1 | Symbols: | D-aminoacid aminotransferase-like PLP-dependent
enzymes superfamily protein | chr5:9678666-9682940
FORWARD LENGTH=2149
Length=2149
Score = 2964 bits (1605), Expect = 0.0
Identities = 1806/1903 (95%), Gaps = 14/1903 (1%)
Strand=Plus/Plus
Query 28 TTTTACATGAGTCCAAGATTCTCTCGGCTGGTTCTTGTTTATAAAGAGGAACACGGACAA 87
|||||||| ||||||||| | | ||| ||||||||||||||||||||| ||||||||| |
Sbjct 45 TTTTACATAAGTCCAAGA-T-TATCGTCTGGTTCTTGTTTATAAAGAGAAACACGGACTA 102
Query 88 AGGCAATAATAGTTTAGTTGAAGCGTCTGTTTGGTTTCAAAGCGATCGTCTTTAATGGAG 147
||||||||||||||| ||||||| | | ||| |||||||| |||||||||||||||||
Sbjct 103 AGGCAATAATAGTTTTGTTGAAG-TTGAGATTGATTTCAAAGGGATCGTCTTTAATGGAG 161
Query 148 GTGATTCACGCATGGTCTGCTCCAAGATCACTCAGCACCACTCTTTTGTACTCCTTTGCC 207
||||| |||||||||||||||||||||||||||||||||||||| ||||||||||||||
Sbjct 162 GTGATACACGCATGGTCTGCTCCAAGATCACTCAGCACCACTCTCATGTACTCCTTTGCC 221
Query 208 CAGAGAGATGACATTGAAGTCCTCGACGAGCCATTATACGCTGCGTTCCTCAAGTCTACG 267
|||||||||||||||||||| || || |||||||||||||||||||||||||||||||||
Sbjct 222 CAGAGAGATGACATTGAAGTGCTTGATGAGCCATTATACGCTGCGTTCCTCAAGTCTACG 281
Query 268 GGTGTTGATAGGCCATACAGAGATGAGCTTCTCTCTAAAATGGAGTGTGATGGAGAAAAG 327
|||||||||||||| |||| |||||||||||||||||| |||||||||||||||||||||
Sbjct 282 GGTGTTGATAGGCCTTACAAAGATGAGCTTCTCTCTAAGATGGAGTGTGATGGAGAAAAG 341
Query 328 GTGGTGAAGGATATCATTTATGGTCCAGGAAAGAAGAAATATAGGTTTTGTAAGCATATA 387
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 342 GTGGTGAAGGATATCATTTATGGTCCAGGAAAGAAGAAATATAGGTTTTGTAAGCATATA 401
Query 388 TCGAAACAGCGACTTCTTGGTTTGCCGAGTGAACTAATGAGTAAAGGAAAGCACTTTATA 447
|||||||||||||||||||||||||| ||||||||||||||| |||| ||||||||||||
Sbjct 402 TCGAAACAGCGACTTCTTGGTTTGCCAAGTGAACTAATGAGTGAAGGGAAGCACTTTATA 461
Query 448 TTGATACGCAATCCCCTTAACATATTGCCTTCTTTTGAGAAGGTACACCCTTCATCGTTT 507
||||||||||||||||| |||||||||||||||||||||||| |||||||||||||||||
Sbjct 462 TTGATACGCAATCCCCTCAACATATTGCCTTCTTTTGAGAAGATACACCCTTCATCGTTT 521
Query 508 CACGAATTGGGATTGGGAGAGTTAGTATCGATATACAGTGATCTCTGTCAGATGGGAACA 567
|||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 522 CACGAATTGGGACTGGGAGAGTTAGTATCGATATACAGTGATCTCTGTCAGATGGGAACA 581
Query 568 CCACCAGCAATCATCGATGCAGATGAGCTCCAGCGAGATCCCGAGGTTACACTACGCAGT 627
|||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
Sbjct 582 CCACCAGCAATCATCGATGCAGATGAGCTCCAGCGAGATCCCGAGGCTACACTACGCAGT 641
Query 628 CTTTGCGATGACTTAGAGATCCCGTTTCAAGCCTCTATGCTTAAATGGGAGGCTGGTCCT 687
||||| ||||||||||||||||| ||||||||||||||||||||||||||||||||||||
Sbjct 642 CTTTGTGATGACTTAGAGATCCCCTTTCAAGCCTCTATGCTTAAATGGGAGGCTGGTCCT 701
Query 688 ATACCAGAAGATGGACTTTGGGCTCCATGGTGGTACAAGACTCTTCACAAATCAACGGGG 747
|||||||||||||||||||||||||||||||||||| |||||||||| |||||||| |||
Sbjct 702 ATACCAGAAGATGGACTTTGGGCTCCATGGTGGTACGAGACTCTTCATAAATCAACAGGG 761
Query 748 TTTTCTTCTCCGAAGAAGTACCCTCAGACTTTCCCTTTAATGCATTACGATTTGCTGGAG 807
|| || || || |||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 762 TTCTCGTCGCCCCAGAAGTACCCTCAGACTTTCCCTTTAATGCATTACGATTTGCTGGAG 821
Query 808 CAATGTTTACCGCTATACAACATTCTCAGGCGCCACGTGAAGCACAAGTCATCTCTCTTG 867
|||||||||||||| ||||||||||| ||| ||||| |||||||||||||||| ||||||
Sbjct 822 CAATGTTTACCGCTGTACAACATTCTGAGGTGCCACATGAAGCACAAGTCATCGCTCTTG 881
Query 868 ACCAGAACCTTAACTCCTCCCAGTCTCCCGGTTCCTGAAAATGCTAAACTTCTTGCTTGG 927
|||| |||||| |||||||||||||||| ||||||||||||||||||||||||||||||
Sbjct 882 TCCAGCACCTTACCTCCTCCCAGTCTCCCAGTTCCTGAAAATGCTAAACTTCTTGCTTGG 941
Query 928 GTTGGTGATGAGATTGTACCACGTGAGATGGCAAAGGTTTCTGTTTTTGACTCGGTCGTG 987
||||||||||||||||| ||||| |||||||| |||||||||||||||||||||||||||
Sbjct 942 GTTGGTGATGAGATTGTGCCACGAGAGATGGCGAAGGTTTCTGTTTTTGACTCGGTCGTG 1001
Query 988 CAAGGTGGTGACTCTGTATGGGAAGGTCTTCGAATATATAAAGGAAAAGTATTCAAGCTT 1047
||||| |||||||||||||||||||||||||| ||||| ||||| |||||||| ||||||
Sbjct 1002 CAAGGCGGTGACTCTGTATGGGAAGGTCTTCGTATATACAAAGGGAAAGTATTTAAGCTT 1061
Query 1048 GAAGAACATCTAGACCGGTTGTCTGATTCAGCAAAGGCTCTGGCTTTCAACAATGTCCCA 1107
||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
Sbjct 1062 GAAGAACATCTAGACCGGTTGTCTGATTCCGCAAAGGCTCTGGCTTTCAACAATGTCCCA 1121
Query 1108 ACCCGCGAGGAGATAAAAGAAGCCATCTTCAGAACTCTCATAACCAATGGGATGTTTGAC 1167
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1122 ACCCGCGAGGAGATAAAAGAAGCCATCTTCAGAACTCTCATAACCAATGGGATGTTTGAC 1181
Query 1168 AATACACATATAAGACTGTCTCTAACTCGAGGCAAAAAGGTCACTTCTGGAATGAGCCCT 1227
||||||||||||||| |||||||||||||||||||||||||||| |||||||||||||||
Sbjct 1182 AATACACATATAAGATTGTCTCTAACTCGAGGCAAAAAGGTCACATCTGGAATGAGCCCT 1241
Query 1228 GCATTTAACCGTTATGGATGTACTTTGATCGTGCTTGCCGAATGGAAGCCTCCGGTATAT 1287
|||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
Sbjct 1242 GCATTTAACCGTTATGGATGTACTTTGATCGTGCTTGCTGAATGGAAGCCTCCGGTATAT 1301
Query 1288 GACAATGATGGCGGAATTGTGCTAGTGACTGCAACTACACGCCGCAATTCACCCAATAAT 1347
||||||||||||||||||||||| |||||||||||||||||||| ||||||||||| |||
Sbjct 1302 GACAATGATGGCGGAATTGTGCTGGTGACTGCAACTACACGCCGGAATTCACCCAACAAT 1361
Query 1348 TTAGATTCCAAGATTCATCACAACAACCTCCTCAATAATATACTTGCAAAGATTGAAAGC 1407
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1362 TTAGATTCCAAGATTCATCACAACAACCTCCTCAATAATATACTTGCAAAGATTGAAAGC 1421
Query 1408 AACAATGCCAAAGTCGATGATGCAATCATGCTTGACAAGGATGGATTTGTGTCTGAAACC 1467
||||||||||| |||||||||||||||||||||||||||||||| |||||||||||||||
Sbjct 1422 AACAATGCCAACGTCGATGATGCAATCATGCTTGACAAGGATGGGTTTGTGTCTGAAACC 1481
Query 1468 AATGCAACCAACATTTTCATGGTGAAGAAAGACCGAGTACTCACCCCTCACGCAGATTAT 1527
|||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||
Sbjct 1482 AATGCAACCAACATTTTCATGGTGAAGAAAGACCGAGTACTCACTCCTCACGCAGATTAT 1541
Query 1528 TGTCTCCCGGGCATTAGC-CGAGCTACTGTTATGGAACTGGTGGTGAAAGAGAACTTCAT 1586
|||||||| ||||||| | ||||||||||| |||||||||||||||||||||||||| ||
Sbjct 1542 TGTCTCCCCGGCATTA-CTCGAGCTACTGTGATGGAACTGGTGGTGAAAGAGAACTTTAT 1600
Query 1587 CTTAGAAGAACGTAGAATTAGCTTGTCGGAATTCCATACAGCTGATGAGGTATGGACTAC 1646
|||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 1601 CTTAGAAGAACGTAGAATCAGCTTGTCGGAATTCCATACAGCTGATGAGGTATGGACTAC 1660
Query 1647 AGGAACTATGGGAGAACTCAGCCCGGTTGTGAAAATTGATGGGCGTGTGATCGGTGAAGG 1706
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1661 AGGAACTATGGGAGAACTCAGCCCGGTTGTGAAAATTGATGGGCGTGTGATCGGTGAAGG 1720
Query 1707 AAAAGTAGGACCGGTAACAAGAAGACTGCAAAGTGCTTACAAGAAACTGACGGATGGTTC 1766
||||||||||||||||||||||||||| |||| |||||||||||||||||||||||||||
Sbjct 1721 AAAAGTAGGACCGGTAACAAGAAGACTTCAAAATGCTTACAAGAAACTGACGGATGGTTC 1780
Query 1767 AGGCGTCCCAATACCTACTTACCAAGAA--C-----C-TTGAACCATGTGTCTGGTCGTA 1818
||| |||||||||||||||||||||||| | | ||||||||||||| || |||||
Sbjct 1781 AGGTGTCCCAATACCTACTTACCAAGAAGTCAAGAACCTTGAACCATGTGTTTGATCGTA 1840
Query 1819 GAACACGAATGGGTAAAAAACATGGCATACGAACAAAACGGCACGTAATACAGAGGAAAG 1878
|||||||||||| ||||||||| |||||||| ||||||||||||| | ||||||||||
Sbjct 1841 GAACACGAATGGA-AAAAAACATTGCATACGAGGAAAACGGCACGTATTTCAGAGGAAAG 1899
Query 1879 CTGTGGTGTTTTAAGATTGTGAATAGAAAGTGCGTGCTGTTTT 1921
||||||||||||| | || ||||||||||||||||||||||||
Sbjct 1900 CTGTGGTGTTTTACGTTTTTGAATAGAAAGTGCGTGCTGTTTT 1942
> AT3G05190.1 | Symbols: | D-aminoacid aminotransferase-like PLP-dependent
enzymes superfamily protein | chr3:1471397-1475784
FORWARD LENGTH=2147
Length=2147
Score = 1827 bits (989), Expect = 0.0
Identities = 1455/1678 (87%), Gaps = 40/1678 (2%)
Strand=Plus/Plus
Query 144 GGAGGTGATTCACGCATGGTCTGCTCCAAGATCACTCAGCACCA-CTCTTTTGTACTCCT 202
||||||||||||| |||||||||| |||||||| |||||||||| | || |||||||||
Sbjct 72 GGAGGTGATTCACTCATGGTCTGCACCAAGATCTCTCAGCACCAGC-CTCATGTACTCCT 130
Query 203 TTGCCCAGAGAGATGACATTGAAGTCCTCGACGAGCCATTATACGC-TGC-GTTCCTCAA 260
|||| |||||| ||||| |||||| |||||||||| || || || | | ||| | ||
Sbjct 131 TTGCTCAGAGACTTGACACTGAAGTTGTCGACGAGCCGTTGTATGCAT-CATTTCTTAAA 189
Query 261 GTCTACGGGTGTTGATAGGCCATACAGAGATG-AGCTTCTCTCTAAAATGGAGTGTGATG 319
| |||| ||| ||||||||||||||||||| | || ||||||| || |||||||| |||
Sbjct 190 G-CTACAGGTTTTGATAGGCCATACAGAGACGAAG-TTCTCTCCAAGATGGAGTGCAATG 247
Query 320 GAGA-AAAGGTGGTGAAGGATATCATTTATGG-TCCAGGAAAGAAGAAATATAGGTTTTG 377
|||| ||| || ||||| ||| |||| ||||| | ||||| ||||| || ||| |||
Sbjct 248 GAGAGAAA-GTTGTGAAAGATGTCATATATGGAT-CAGGATCTAAGAAGTACCGGTATTG 305
Query 378 TAAGCATATATCGAAACAGCGACTTCTT-GGTTTGCCGAGTGAACTAATGAGTAAAGGAA 436
||||||||| ||||| ||||| ||| || |||||||| |||||||| ||||||| |||||
Sbjct 306 TAAGCATATCTCGAAGCAGCGGCTT-TTCGGTTTGCCAAGTGAACTGATGAGTAGAGGAA 364
Query 437 AGCACTTT-ATATTGATACGCAATCCCCTTAACATATTGCCTTCTTTTGAGAAGGTACAC 495
|||| ||| ||||| ||||| ||||| ||||||||||||||||| |||||||||||||||
Sbjct 365 AGCA-TTTCATATTAATACGAAATCCTCTTAACATATTGCCTTCCTTTGAGAAGGTACAC 423
Query 496 CCTTCATCGTTTCACGAATTGGGATTGGGAGAGTTAGTATCGATATACAGTGATCTCTGT 555
||| |||| |||| |||||| || ||||| || ||||| |||||||||||||||||||||
Sbjct 424 CCTCCATCATTTCTCGAATTAGGCTTGGGGGAATTAGTGTCGATATACAGTGATCTCTGT 483
Query 556 CAGATGGGAACACCACCAGCAATCATCGATGCAGATGAGCTCCAGCGAGATCCCGAGGTT 615
||||||||||||||||||||| |||||||||| |||||||| ||||||||||||||| |
Sbjct 484 CAGATGGGAACACCACCAGCAGTCATCGATGCGGATGAGCTTCAGCGAGATCCCGAGACT 543
Query 616 ACACTACGCAGTCTTTGCGATGACTTAGAGATCCCGTTTCAAGCCTCTATGCTTAAATGG 675
||||| ||| ||||||| ||||| || ||||| || ||||||||||||||||||||||||
Sbjct 544 ACACTTCGCGGTCTTTGTGATGATTTGGAGATTCCATTTCAAGCCTCTATGCTTAAATGG 603
Query 676 GAGGCTGGTCCTATACCAGAAGATGGACTTTGGGCTCCATGGTGGTACAAGACTCTTCAC 735
|||||||||||||||||||||||||| | ||||| |||||||||||||||| | |||||
Sbjct 604 AAGGCTGGTCCTATACCAGAAGATGGAGTATGGGCACCATGGTGGTACAAGAGTGTTCAC 663
Query 736 AAATCAACGGGGTTT-TCTTCTCCGAAGAAGTACCCTCAGACTTTCCCTTTAAT-GCATT 793
||||||| |||||| || ||||| |||||||| |||| ||| |||||||| | |||||
Sbjct 664 GAATCAAC-GGGTTTCTCATCTCCAAAGAAGTATCCTCGGACATTCCCTTT-GTCGCATT 721
Query 794 ACGATTTGCTGGAGC-AATGTTTACCGCTATACAACATTCTCAGGCGCCACGTGAAGCAC 852
||||||||||||||| || || ||||||| ||||||||||||||| ||||||||||||||
Sbjct 722 ACGATTTGCTGGAGCGAA-GTCTACCGCTTTACAACATTCTCAGGAGCCACGTGAAGCAC 780
Query 853 AAG-TCATCTCTCTTGACCAGAACCTTAACTCCTCCCAGTCTCCCGGTTCCTGAAAATGC 911
|| || || ||||||| || |||| ||||||| |||||||| ||||||||||||||
Sbjct 781 -AGTTCCTCCCTCTTGAGTAGTCCCTTGCCTCCTCCTAGTCTCCCAGTTCCTGAAAATGC 839
Query 912 TAAACTTCTTGCTTGGGTTGGTGATGAGATTGTACCACGTGAGATGGCAAAGGTTTCTGT 971
||||||||||| ||||| ||||| |||||| | || ||||| |||||||||||||||||
Sbjct 840 AAAACTTCTTGCATGGGTCGGTGACGAGATTTTGCCTCGTGAAATGGCAAAGGTTTCTGT 899
Query 972 TTTTGACTCGGTCGTGCAAGGTGGTGACTCTGTATGGGAAGGTCTTCGAATATATA-AAG 1030
|||||||||||| || ||||| |||||||||||||||||||| ||||| || | || |||
Sbjct 900 TTTTGACTCGGTTGTTCAAGGCGGTGACTCTGTATGGGAAGGACTTCGGAT-T-TACAAG 957
Query 1031 GAAAAG-TATTCAAGCTTGAAGAACATCTAGACCGGTTGTCTGATTCAGCAAAGGCTCTG 1089
| |||| ||||||||||||| ||||||||||| ||||||| |||||||||||||||||||
Sbjct 958 GGAAAGATATTCAAGCTTGAGGAACATCTAGATCGGTTGTTTGATTCAGCAAAGGCTCTG 1017
Query 1090 GCTTTCAACAATGTCCCAACCCGCGAGGAGATAAAAGAAGCCATCTTCAGAACTCTCATA 1149
|||||| | ||||| ||| | || || ||| |||||||||| |||||||||||||| |||
Sbjct 1018 GCTTTCGATAATGTTCCAGCGCGTGAAGAGGTAAAAGAAGCAATCTTCAGAACTCTTATA 1077
Query 1150 ACCAATGGGATGTTTGACAATACACATATAAGACTGTCTCTAACTCGAGGCAAAAAGGTC 1209
|||||||| |||||||||||||||||||| || |||||||||||||||||||||||||
Sbjct 1078 ACCAATGGTATGTTTGACAATACACATATCAGGTTGTCTCTAACTCGAGGCAAAAAGGTT 1137
Query 1210 ACTTCTGGAATGAGCCCTGCATTTAACCGTTATGGATGTACT-TTGATCGTGCTTGCCGA 1268
|||||||||||||||||||||| ||| || ||||| || ||| || || |||||||||||
Sbjct 1138 ACTTCTGGAATGAGCCCTGCATATAATCGATATGGGTGCACTCTT-ATTGTGCTTGCCGA 1196
Query 1269 ATGGAAGCCTCCGGTATATGACAATGATGGCGGAATTGTGCTAGTGACTGCAACTACACG 1328
||||||||||||||||||||||||||| || ||||||||||| ||||||||||| |||||
Sbjct 1197 ATGGAAGCCTCCGGTATATGACAATGAAGGTGGAATTGTGCTGGTGACTGCAACCACACG 1256
Query 1329 CCGCAATTCACCCAATAATTTAGATTCCAAGATTCATCACAACAACCTCCTCAATAATAT 1388
||||||||| || |||||||| |||||||||||||||||||||||||| |||||||| ||
Sbjct 1257 CCGCAATTCGCCTAATAATTTGGATTCCAAGATTCATCACAACAACCTTCTCAATAACAT 1316
Query 1389 ACTTGCAAAGATTGAAAGCAACAATGCCAAAGTC-GATGATGCAATCATGCTTGACAAGG 1447
||| |||||||||||||| ||||| | || | | | ||| || || ||||||||||| |
Sbjct 1317 ACTAGCAAAGATTGAAAGTAACAACACGAATG-CTGCTGACGCCATTATGCTTGACAAAG 1375
Query 1448 ATGGATT-TGTGTCTGAAACCAATGCAACCAACATTTTCATGGTGAAGAAAGACCGAGTA 1506
|||| || |||||||||||||||||||||||||||||||||||||||||||| | | |||
Sbjct 1376 ATGG-TTATGTGTCTGAAACCAATGCAACCAACATTTTCATGGTGAAGAAAGGCTGTGTA 1434
Query 1507 CTCACCCCTCACGCAGATTATTGTCTCCCGGGCATTAGCC-GAGCTACTGTTATGGAACT 1565
|| || |||||||||||||| |||||||| ||||| | || |||| ||||| ||||||||
Sbjct 1435 CTTACTCCTCACGCAGATTACTGTCTCCCTGGCATAA-CCAGAGCAACTGTAATGGAACT 1493
Query 1566 GGTGGTGAAAGAGAACTTCATCTTAGAAGAACGTAGAATTAG-CTTGTCGGAATTCCATA 1624
|||||||||||||||||||||||||||||| | ||||| || || || ||||||||||
Sbjct 1494 TGTGGTGAAAGAGAACTTCATCTTAGAAGAAAGAAGAATCAGTCTC-TCCGAATTCCATA 1552
Query 1625 CAGCTGATGAGGTATGGACTACAGGAACTATGGGAGAACTCAGCCCGGTTGTGAAAATTG 1684
||||| ||||||||||||| |||||||||||||||||||| ||||||||||||||||| |
Sbjct 1553 CAGCTAATGAGGTATGGACAACAGGAACTATGGGAGAACTAAGCCCGGTTGTGAAAATCG 1612
Query 1685 ATGGGCGTGTGATCGGTGAAGGAAAAGTAGGACCGGTAACAAGAAGACTGCAAAGTGCTT 1744
||||||||||||| ||||| || ||||| ||||||||||| |||| |||||||| ||||
Sbjct 1613 ATGGGCGTGTGATTGGTGACGGCAAAGTTGGACCGGTAACGAGAACACTGCAAAACGCTT 1672
Query 1745 ACAAGAAACTGACGGATGG-TTCAGGCGTCCCAATACCTACTTACCAAGAACCTTGAA 1801
||||||||||||| || || |||||| || || ||||||||||| |||||||| ||||
Sbjct 1673 ACAAGAAACTGACAGA-GGATTCAGGTGTGCCGATACCTACTTATCAAGAACCCTGAA 1729
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 98192266020
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5