BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg491471
Length=1759
Score E
Sequences producing significant alignments: (Bits) Value
AT4G32520.1 | Symbols: SHM3, AtSHMT3 | serine hydroxymethyltran... 2651 0.0
> AT4G32520.1 | Symbols: SHM3, AtSHMT3 | serine hydroxymethyltransferase
3 | chr4:15689464-15692857 REVERSE LENGTH=1924
Length=1924
Score = 2651 bits (1435), Expect = 0.0
Identities = 1564/1627 (96%), Gaps = 6/1627 (0%)
Strand=Plus/Plus
Query 73 TTCTACCTTTCCGGAGTAGAGAGAAAGAATGCAAGCTTGTTGTGGTGGTACTTCGATGGA 132
||||||||||||||||||||||||||| |||||||||||||||||||||| ||||||||
Sbjct 130 TTCTACCTTTCCGGAGTAGAGAGAAAG-ATGCAAGCTTGTTGTGGTGGTAATTCGATGGC 188
Query 133 TTCTCTTCAGCAACCTGGACGGGTTCAAGGATCTGTCTTTCCCCCACTAATGCCTCCAGT 192
|||||||||||||||||||||||||||||||||||||||||||||| | |||||||||||
Sbjct 189 TTCTCTTCAGCAACCTGGACGGGTTCAAGGATCTGTCTTTCCCCCAATTATGCCTCCAGT 248
Query 193 GACCAAATTTTCTCAGCAATTGAAGTTCAATTTTTCAAAACCTTTCCGTTCTTCTTTTCT 252
|||||||||||||||||| |||||||||||| |||||||||||||||||||||| |||||
Sbjct 249 GACCAAATTTTCTCAGCAGTTGAAGTTCAATATTTCAAAACCTTTCCGTTCTTCATTTCT 308
Query 253 GGAAAGAAACTTGGTCTTTGAAAGGAGAGCATCTTCAGTCTCGCTACCTAATGTCGAGAT 312
| |||||||| |||||| ||||| ||||||||||||||||||||||||||||||||||||
Sbjct 309 GAAAAGAAACCTGGTCTCTGAAATGAGAGCATCTTCAGTCTCGCTACCTAATGTCGAGAT 368
Query 313 GAGCAGCAAAGAGATCGCTTTTGCGGACTATGGTCTTGGTGAAGTTGATCCAGAGGTCCG 372
||||||||||| ||| |||||| ||||||||||||||||||||||||||||||||||||
Sbjct 369 AAGCAGCAAAGAAATCCCTTTTGAGGACTATGGTCTTGGTGAAGTTGATCCAGAGGTCCG 428
Query 373 AACGATCATCACAAAGGAGAAAGATAGACAATTCAGGAGCTTGGAGCTTATTGCGTCTGA 432
||| |||||||||||||||||||||||||||||||||||||||||||||||||| |||||
Sbjct 429 AACCATCATCACAAAGGAGAAAGATAGACAATTCAGGAGCTTGGAGCTTATTGCATCTGA 488
Query 433 GAATTTCACGTCTCGAGCTGTTATGGAGGCGGTTGGATCATGCCTTACCAACAAGTATTC 492
|||||||||||||||||| ||||||||||||||||| |||||||||||||||||||||||
Sbjct 489 GAATTTCACGTCTCGAGCAGTTATGGAGGCGGTTGGCTCATGCCTTACCAACAAGTATTC 548
Query 493 TGAGGGACTGCCTGGTAAAAGATATTATGGTGGCAATGAGTACATCGATCAGTTGGAGAC 552
||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||
Sbjct 549 TGAGGGACTGCCTGGTAAAAGATATTATGGTGGCAATGAGTACATTGATCAGTTGGAGAC 608
Query 553 ACTTTGCCAAAACCGGGCTTTAGCAGCCTTCCGCTTAGATTCTACGAAATGGGGGGTTAA 612
|||||||| ||||||||||| ||||||||||||||||||||||||||||||||||||||
Sbjct 609 GCTTTGCCAGAACCGGGCTTTGGCAGCCTTCCGCTTAGATTCTACGAAATGGGGGGTTAA 668
Query 613 TGTTCAGCCGTTATCTGGTTCACCTGCAAACTTTGCTGTCTACACTGCGATACTTAGCCC 672
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 669 TGTTCAGCCGTTATCTGGTTCACCTGCAAACTTTGCTGTCTACACTGCGATACTTAGCCC 728
Query 673 TCATGATCGGATCATGGGTTTGGATTTACCTCACGGTGGTCATCTGTCGCATGGGTTCAT 732
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 729 TCATGATCGGATCATGGGTTTGGATTTACCTCACGGTGGTCATCTGTCGCATGGGTTCAT 788
Query 733 GACTGCTAAAAGGCGTGTGTCTGGAACTTCAATATACTTTGAGTCCATGCCTTATCGACT 792
||| |||||||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 789 GACCGCTAAAAGGCGTGTATCTGGAACTTCAATATACTTTGAGTCCATGCCTTATCGACT 848
Query 793 TGATGAATCAACAGGCATTGTTGACTACGATATGCTAGAGAAAACTGCTACACTCTTCAG 852
|||||||||||||||||||||||||||||||||||| ||||||||||||||||| |||||
Sbjct 849 TGATGAATCAACAGGCATTGTTGACTACGATATGCTCGAGAAAACTGCTACACTTTTCAG 908
Query 853 ACCAAAGCTTATTATCGCAGGAGCTAGTGCGTACAGCCGAGATTTTGACTATCCTCGCAT 912
|||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
Sbjct 909 ACCAAAGCTTATTATCGCAGGAGCTAGTGCATACAGCCGAGATTTTGACTATCCTCGCAT 968
Query 913 GAGGAAGATCGCAGACTCAATTGGTGCTTTTCTGATGATGGATATGGCTCATATCAGCGG 972
||||||||||||||||||| ||||||||||||||||||||||||||||||||||||| ||
Sbjct 969 GAGGAAGATCGCAGACTCAGTTGGTGCTTTTCTGATGATGGATATGGCTCATATCAGTGG 1028
Query 973 GCTTGTGGCTGCTTCTGTGGTAGCTGACCCTTTTGAGTATTGTGATATTGTTACAACAAC 1032
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1029 GCTTGTGGCTGCTTCTGTGGTAGCTGACCCTTTTGAGTATTGTGATATTGTTACAACAAC 1088
Query 1033 AACTCACAAGTCTTTGAGAGGTCCGAGAGGTGGCATGATCTTCTTCAGGAAGGATCCAAT 1092
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1089 AACTCACAAGTCTTTGAGAGGTCCGAGAGGTGGCATGATCTTCTTCAGGAAGGATCCAAT 1148
Query 1093 TAATGGGGTTGATCTTGAATCTGCCGTAAACAATGCTGTCTTCCCTGGTTTGCAGGGTGG 1152
||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||
Sbjct 1149 TAATGGGGTTGATCTTGAATCTGCCGTGAACAATGCTGTCTTCCCTGGTTTGCAGGGTGG 1208
Query 1153 CCCTCATAACCACACAATTGGAGGATTAGCAGTCTGCTTGAAACACGCACAATCACTGGA 1212
|||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
Sbjct 1209 CCCTCATAACCACACAATTGGAGGATTAGCAGTCTGCTTGAAACACGCACAATCACCGGA 1268
Query 1213 ATTCAAGGCTTACCAGAAAAGAGTTGTGTCCAACTGTAGAGCTCTAGCTAACCGATTGGT 1272
||||||||| |||||||||||||||||||| |||||||||||||||||||||||||||||
Sbjct 1269 ATTCAAGGCGTACCAGAAAAGAGTTGTGTCTAACTGTAGAGCTCTAGCTAACCGATTGGT 1328
Query 1273 TGAACTCGGGTTTAAGCTGGTTTCTGGTGGCAGCGATAACCACTTGGTTCTCGTTGATCT 1332
|||||||||||||||||| ||||||||||||||||||||||||||||||||||| |||||
Sbjct 1329 TGAACTCGGGTTTAAGCTAGTTTCTGGTGGCAGCGATAACCACTTGGTTCTCGTCGATCT 1388
Query 1333 TAGACCCATGGTAAGCATGGATGGTGCTCGGGTTGAGAAAATCTTAGACATGGCATCTAT 1392
||||||||||| | ||||||||||| ||||||||||||||||||||||||||||||||
Sbjct 1389 TAGACCCATGG---GTATGGATGGTGCGCGGGTTGAGAAAATCTTAGACATGGCATCTAT 1445
Query 1393 TACTCTTAACAAGAACTCTGTCCCTGGGGATAAAAGTGCGTTGGTTCCAGGAGGAATAAG 1452
|||||| ||||||||||||||||||||||| |||||||||||||| ||||| ||||||||
Sbjct 1446 TACTCTCAACAAGAACTCTGTCCCTGGGGACAAAAGTGCGTTGGTACCAGGGGGAATAAG 1505
Query 1453 GATAGGATCGCCTGCGATGACGACAAGAGGACTGTCAGAGAAAGACTTTGTAGCAGTCGC 1512
||||||||| || |||||||||||||||||||||||||||||||||||||||| ||| ||
Sbjct 1506 GATAGGATCCCCCGCGATGACGACAAGAGGACTGTCAGAGAAAGACTTTGTAGTAGTGGC 1565
Query 1513 TGACTTCATCAAGGAAGGTGTGGAGATAACAATGGAAGCCAAGAAAGCTACGCCGGGTTC 1572
|||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
Sbjct 1566 TGACTTCATCAAGGAAGGTGTGGAGATAACAATGGAAGCCAAGAAAGCCGCGCCGGGTTC 1625
Query 1573 AAAGCTGCAAGATTTCACCAAGTTCGTGACTTCTCCTGACTTTCCATT-AATAGAGAGAG 1631
|||||| |||||||||| |||||||||||| |||||||| |||||||| || ||||||||
Sbjct 1626 AAAGCTTCAAGATTTCAACAAGTTCGTGACATCTCCTGAGTTTCCATTGAA-AGAGAGAG 1684
Query 1632 TGAAGAGTCTCAAGGAAAGAGTGGAATCCTTCACCTCTCATTTTCCCATTCCTGGCGTTT 1691
|||||||||| ||||| ||||||||| ||||||| |||| ||||||||||||||||||||
Sbjct 1685 TGAAGAGTCTAAAGGAGAGAGTGGAAACCTTCACATCTCGTTTTCCCATTCCTGGCGTTT 1744
Query 1692 AAGCTAT 1698
|||||||
Sbjct 1745 AAGCTAT 1751
Score = 91.6 bits (49), Expect = 2e-17
Identities = 66/74 (89%), Gaps = 2/74 (3%)
Strand=Plus/Plus
Query 1 TCCCGTAAAACCCTAAACATAGTCACTTTATCGTCTCTCCTCCACCAAATCCTTCAATCT 60
|||||||||||||||||||||||||||| |||||||| | ||||| ||| ||||||||
Sbjct 26 TCCCGTAAAACCCTAAACATAGTCACTTCATCGTCTC-C-TCCACTGTATCATTCAATCT 83
Query 61 CCGTCTTCCTTCTT 74
| ||||||||||||
Sbjct 84 CTGTCTTCCTTCTT 97
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 86997544485
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5