BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg491858
Length=2630
Score E
Sequences producing significant alignments: (Bits) Value
AT4G29000.1 | Symbols: | Tesmin/TSO1-like CXC domain-containin... 3376 0.0
AT2G20110.2 | Symbols: | Tesmin/TSO1-like CXC domain-containin... 736 0.0
> AT4G29000.1 | Symbols: | Tesmin/TSO1-like CXC domain-containing
protein | chr4:14293513-14297066 FORWARD LENGTH=2720
Length=2720
Score = 3376 bits (1828), Expect = 0.0
Identities = 2140/2288 (94%), Gaps = 32/2288 (1%)
Strand=Plus/Plus
Query 364 GAGATGGGTGAAGACGGCGGAGGAGGCGAATTCCCGCCAAAAAAGGATGGTGTTGAAGAG 423
||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
Sbjct 442 GAGATGGGTGAAGACGGCGGAGGAGGCGAATTCCCGCCAAAAAAGGACGGTGTTGAAGAG 501
Query 424 GGTTTTCCGACGAAGAAGCCAGCAAGACAGCTTGACTTCACTGGTGGCTCTGATGAACAA 483
|||||||||||||||||||||||||| ||||| ||||||||||||||||| ||||||||
Sbjct 502 GGTTTTCCGACGAAGAAGCCAGCAAGGCAGCTCGACTTCACTGGTGGCTCCGATGAACAT 561
Query 484 TCACTGTCTAAAGCAGTGGCTCCTACCGTCGTAGCTACGGCGGTGAAACCCGTCGTTACT 543
|||||||||||| ||| |||||||||||||||||||||| ||||||||||| || ||| |
Sbjct 562 TCACTGTCTAAACCAGCGGCTCCTACCGTCGTAGCTACGTCGGTGAAACCCATCATTAGT 621
Query 544 AGCTCCATCCCTTCGACGATACGGCCGGGTGTGACCATTGCAA----TC-GG----TCAG 594
||||| ||||||||||||||||||||||| ||||||| |||| || || ||||
Sbjct 622 AGCTCTGTCCCTTCGACGATACGGCCGGGTATGACCATAGCAATCGGTCAGGTTACTCAG 681
Query 595 GTACGACCGACGTTACCAATGGCGACGAC---GTCAAATCCGCCGTCACAATCACAAATC 651
||||||||||||||||||||||||||||| |||||||||||||||||| |||||||||
Sbjct 682 GTACGACCGACGTTACCAATGGCGACGACGATGTCAAATCCGCCGTCACAGTCACAAATC 741
Query 652 CTTAACGCGCCAATCAGGCATCCAAAACCGGAATCTCCAAAAGCTAGAGGTCCAAGGCCT 711
||||||||||||| |||||||| | ||||||||||||||||||||||||||||||||||
Sbjct 742 GTTAACGCGCCAATTAGGCATCCGATACCGGAATCTCCAAAAGCTAGAGGTCCAAGGCCT 801
Query 712 ATTGTGGAAGGTAGAGATGGAACTCCTCAGAAGAAGAAGCAGTGTAACTGTAAACACTCA 771
| ||||||||||||||||||||| ||||| ||||||||||||||||||||||||||||||
Sbjct 802 AATGTGGAAGGTAGAGATGGAACCCCTCAAAAGAAGAAGCAGTGTAACTGTAAACACTCA 861
Query 772 CGCTGCTTGAAACTGTACTGTGAATGCTTTGCATCCGGAACATATTGTGACGGTTGTAAC 831
|||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 862 CGCTGCTTGAAATTGTACTGTGAATGCTTTGCATCCGGAACATATTGTGACGGTTGTAAC 921
Query 832 TGTGTAAATTGTTTCAACAATGTTGATAATGAACCTGCAAGACGAGAAGCTGTGGAAGCA 891
|||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
Sbjct 922 TGTGTAAATTGTTTCAACAATGTTGATAATGAACCTGCTAGACGAGAAGCTGTGGAAGCA 981
Query 892 ACTCTGGAGCGGAATCCATTTGCGTTCAGGCCTAAAATTGCTAGCAGTCCACATGGTGTG 951
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
Sbjct 982 ACTCTGGAGCGGAATCCATTTGCGTTCAGGCCTAAAATTGCTAGCAGTCCACATGGTGGG 1041
Query 952 CGGGATAAAAGGGAGGACATTGGTGAAGTTGTGTTGTTAGGGAAACATAACAAAGGATGC 1011
||||||||||||||||| || |||||||||||||||||||||||||||||||||||||||
Sbjct 1042 CGGGATAAAAGGGAGGATATCGGTGAAGTTGTGTTGTTAGGGAAACATAACAAAGGATGC 1101
Query 1012 CACTGCAAGAAATCGGGATGCCTTAAGAAGTATTGTGAGTGCTTTCAAGCAAACATTCTT 1071
|||||||| ||||| |||||||||||||||||||| ||||||||||||||||| ||||||
Sbjct 1102 CACTGCAAAAAATCAGGATGCCTTAAGAAGTATTGCGAGTGCTTTCAAGCAAATATTCTT 1161
Query 1072 TGTTCTGAGAACTGCAAATGCTTGGATTGCAAAAACTTTGAAGGAAGTGAAGAGAGACAA 1131
||||| |||||||||||||||||||||||||| |||||||||||||||||||||||||||
Sbjct 1162 TGTTCCGAGAACTGCAAATGCTTGGATTGCAAGAACTTTGAAGGAAGTGAAGAGAGACAA 1221
Query 1132 GCTCTGTTTCACGGTGAACATGCCAACCACATGGCTTATCTTCAACAAGCAGCAAATGCA 1191
||| ||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
Sbjct 1222 GCTTTGTTTCACGGTGAACATTCCAACCACATGGCTTATCTTCAACAAGCAGCAAATGCA 1281
Query 1192 GCCATTACTGGAGCTGTTGGTTCCTCCGGCTTTGCTCCTTCTCCAGCACCTAAGAGAAGG 1251
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1282 GCCATTACTGGAGCTGTTGGTTCCTCCGGCTTTGCTCCTTCTCCAGCACCTAAGAGAAGG 1341
Query 1252 AAAGGTCAAGAGATTTTGTTCAACCAAGCAACCAAAGATTCATCTAGACTTGGCCAGTTT 1311
||||||||||||||||||||||||||||||| |||||||||||||| ||| |||| |||
Sbjct 1342 AAAGGTCAAGAGATTTTGTTCAACCAAGCAATTAAAGATTCATCTAGGCTTAGCCACTTT 1401
Query 1312 CCACGGGTAAATAGTGGAAGAGCTAGTGGACCAACCTCAGGCTCATCTCCGTCACCTGTT 1371
|||| |||||||| ||||||| || ||||||||||||||||| ||||||| |||||||||
Sbjct 1402 CCACAGGTAAATAATGGAAGAACTGGTGGACCAACCTCAGGCACATCTCCATCACCTGTT 1461
Query 1372 TCTCGTGCTGGTGGCAATGCATCGTCAGCTCCATCAAAGTTTGTATATAGGTCCCTTTTA 1431
|||||||||||||||||||||||||||| |||||||||||||||||| ||||||||||||
Sbjct 1462 TCTCGTGCTGGTGGCAATGCATCGTCAGTTCCATCAAAGTTTGTATACAGGTCCCTTTTA 1521
Query 1432 GCAGATATAATCCAACCGCATGATGTTAGAGCACTTTGCTCTGTTTTAGTCGCTGTAGCC 1491
||||||||||||||||| |||||||| |||||||||||||||||||||||| ||||||||
Sbjct 1522 GCAGATATAATCCAACCACATGATGTGAGAGCACTTTGCTCTGTTTTAGTCACTGTAGCC 1581
Query 1492 GGAGAAGCAGCAAAGACATCAACAGATAAAAGAAATGAAATAGAAAATCGTGTGGAAGAT 1551
|||||||||||||||||||||||||||||||||||||||||||||||||| ||||| |||
Sbjct 1582 GGAGAAGCAGCAAAGACATCAACAGATAAAAGAAATGAAATAGAAAATCGGGTGGACGAT 1641
Query 1552 CAAACAGAAACTTCTTTAGCTTCTTCTGCACAAGATCAGCCCCAAGGCGATAATGATGCA 1611
||||||||||||||||||||||||||||||||||||||||| |||||| |||| |||||
Sbjct 1642 CAAACAGAAACTTCTTTAGCTTCTTCTGCACAAGATCAGCCTCAAGGCAATAACAATGCA 1701
Query 1612 GCTGATATGGAGATGGTTGCAACTGATGGGAATCAAGCTGATAAGTCTGGACCAGAGGAA 1671
|||||| |||||||||||||||||||| ||||||||||||||||||||||||||||||
Sbjct 1702 GCTGATGTGGAGATGGTTGCAACTGATCATAATCAAGCTGATAAGTCTGGACCAGAGGAA 1761
Query 1672 TCCAATTCAGATGGTGCCGATGCCTCGAAAGGGAATCCACTATCTCCAGCAACTTTGGCT 1731
|||||||||||||||| |||||||| |||| || || |||||||||||||||||||||
Sbjct 1762 TCCAATTCAGATGGTGTTGATGCCTCTAAAGTGACCCCGCTATCTCCAGCAACTTTGGCT 1821
Query 1732 TTGATGTGCGATGAACAAGACACTATCTTCATGGTGGCAGCACCTTCGCCTAATGG-TGC 1790
|||||||||||||||||||||||||||||||||||||| |||||||| |||||||| | |
Sbjct 1822 TTGATGTGCGATGAACAAGACACTATCTTCATGGTGGCTGCACCTTCACCTAATGGCT-C 1880
Query 1791 AGTCGACCCCGGTGGCCGCAGAACGAACTCACAGGGCCAGTCAGAGATTTACGCAGAGCA 1850
|||||| ||| |||| ||||| | |||||||||||||||||||| ||||||||||||||
Sbjct 1881 AGTCGATCCCAATGGCTGCAGACCAAACTCACAGGGCCAGTCAGAAATTTACGCAGAGCA 1940
Query 1851 GGAGAGACTGGTATTAACCAAATTCAGAGACTGCCTTAGTCGACTTATCTCTTACGCAGA 1910
|||||||||||| ||||||||||||||||||||||||| |||||||||||||||||||||
Sbjct 1941 GGAGAGACTGGTGTTAACCAAATTCAGAGACTGCCTTAATCGACTTATCTCTTACGCAGA 2000
Query 1911 AATAAAAGAATCAAAGTGTTTATCTTTAGCAAGAATGCACATACAGCCACCTGCAACCGC 1970
|||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
Sbjct 2001 AATAAAAGAATCAAAGTGTTTATCTTTAGCAAGAATGCACATACAGCCACCTGCAATCGC 2060
Query 1971 GACTGTGAAAACCGAGAATGGGGTTCAACAGCAAGTACCAATTGTGAATGGAGCTTCGCG 2030
|||||| ||||||||||||||| |||||||||||||||||||||||||||||||||||||
Sbjct 2061 GACTGTAAAAACCGAGAATGGGATTCAACAGCAAGTACCAATTGTGAATGGAGCTTCGCG 2120
Query 2031 AACCAATTCTCAACCTACACTCAACAAACCGCAGCCTATGCAGCTGATAAACACAACATc 2090
|||||||| ||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 2121 AACCAATTGTCAACCTACACTCAACAAACCGCAGCCTATGCAGCTGATGAACACAACATC 2180
Query 2091 agcatcagcagcagcagcagcTGCAACAAACACTCATCATCTTCATAAACCTCCAGCTTT 2150
|||| || ||||| | |||| |||||||||||| |||||||| ||||||||||||||
Sbjct 2181 AGCAGCACCAGCA--A-CAGC---AACAAACACTCAACATCTTCAAAAACCTCCAGCTTT 2234
Query 2151 ATCAGAGAAGAAAGACCCCTGAATCAGATAGATTAT-A--CTCTACTTAGGACAGATAAT 2207
|||||||||||||||||||||||||||||||||||| | |||||||||||||||| |||
Sbjct 2235 ATCAGAGAAGAAAGACCCCTGAATCAGATAGATTATTATACTCTACTTAGGACAGAAAAT 2294
Query 2208 AACTTACCAGAGAGAACAAGCTGAAATCTGCCTCACGACTGCCCTAT-TTATCAGGTCAT 2266
||||||||||||||| || ||||||||||||||||| || ||||||| ||||||||| ||
Sbjct 2295 AACTTACCAGAGAGAGCACGCTGAAATCTGCCTCACCACCGCCCTATATTATCAGGTAAT 2354
Query 2267 GCCACCATAATTTTTCTTTCTCACTGGCGGTTGTTGCGGCTTCAGCGCATTGCCAAGTGA 2326
||||| |||||||||||||||| |||||||||||||||||||| ||||||| || ||||
Sbjct 2355 CCCACCGTAATTTTTCTTTCTCAGTGGCGGTTGTTGCGGCTTCAACGCATTGGCAGGTGA 2414
Query 2327 TGAGAGAGAGTAATAGACACAAGCTTTTATTGTGTCGTTAAGctttcttctcacttttct 2386
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2415 TTAGAGAGAGTAATAGACACAAGCTTTTATTGTGTCGTTAAGCTTTCTTCTCACTTTTCT 2474
Query 2387 ctttcttttcttAAAAAGTCCCCTCTTTAAAGCCCCT-GTGGCTGTTGTAGATTTTGGAG 2445
||||||||||||| |||||| |||||||||||||||| ||||||| |||||||||||| |
Sbjct 2475 CTTTCTTTTCTTATAAAGTCTCCTCTTTAAAGCCCCTTGTGGCTGGTGTAGATTTTGGTG 2534
Query 2446 AGCTTTGTTTCTTCTACTACACGAGCTTTTTGTTAGTTTTTTG---GTGTTCAGTATTAC 2502
||||||||||||| |||||||||| |||||||||||||||| ||||||||||| ||
Sbjct 2535 AGCTTTGTTTCTT-TACTACACGAATTTTTTGTTAGTTTTTTTTTTGTGTTCAGTATCAC 2593
Query 2503 TTTTTCTGTTGATGGTTTAGCTGTGGAACATCCTTGTTTACTGTGTCTGATTATGATGAT 2562
||||| |||||||||||||||||||||||| |||||||||||||||| |||||||||
Sbjct 2594 TTTTTATGTTGATGGTTTAGCTGTGGAACACCCTTGTTTACTGTGTCGGATTATGAT--- 2650
Query 2563 CTGAGATGTTTCTCAATCAGCTTCTGTAGGATTTGAAAGTAATATATAATACATCCCTAA 2622
||||||||||||||||||||||||||||||||||||||||||||||||||| |||| |||
Sbjct 2651 CTGAGATGTTTCTCAATCAGCTTCTGTAGGATTTGAAAGTAATATATAATAAATCCTTAA 2710
Query 2623 TTTGGTTA 2630
|||| |||
Sbjct 2711 TTTGATTA 2718
Score = 331 bits (179), Expect = 2e-89
Identities = 310/366 (85%), Gaps = 38/366 (10%)
Strand=Plus/Plus
Query 1 ATGTTagagagagggaggaggagagatggtgtgagagaagaagaagaaCAAAACCGAAAG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1 ATGTTAGAGAGAGGGAGGAGGAGAGATGGTGTGAGAGAAGAAGAAGAACAAAACCGAAAG 60
Query 61 TA--ttttt-tgcttttaa--ttt-ttcgt--tttt----aatt--t-t-A---C--CAA 99
|| ||||| ||||||||| ||| || | |||| |||| | | | | |
Sbjct 61 TATTTTTTTGTGCTTTTAATTTTTGTTTTTAATTTTACCAAATTCCTCTGAGGGCTTCTT 120
Query 100 ATTC--C-TCT--GAGGGcttcttcttcttcttcttc---AAGAGCTAAAACCCAATAGA 151
||| | ||| | ||||||||||||||||||| |||||||||||||||||||
Sbjct 121 CTTCTTCTTCTCCGTCTTCTTCTTCTTCTTCTTCTTCGTTTAGAGCTAAAACCCAATAGA 180
Query 152 ACCCTAATTCTCAATTCT-CTTTAAAGCTTATCCCTTtctctctctctctctA-ATCACG 209
|||||||||||| | ||| | ||||||||||||||||| ||||||||| | || || |||
Sbjct 181 ACCCTAATTCTC-A-TCTCCCTTAAAGCTTATCCCTTT-TCTCTCTCTATATATAT-ACG 236
Query 210 CGGATCAAATTCAAAGTCTCAGTTTTTGAGCTTTTCTTAACAGCCGCCGAATCGGTTTGT 269
|||||||||||||||||||||||||||||||||| ||||||||||||||||||| |||||
Sbjct 237 CGGATCAAATTCAAAGTCTCAGTTTTTGAGCTTT-CTTAACAGCCGCCGAATCGATTTGT 295
Query 270 GATTTTCTCGCTCCGGCGAGATTCGGATCTGGATTTT-CTTAGCTGATTTGTGGGAATTT 328
||||||| |||||||| |||||||||||||||||||| | ||||||||||| |||||||
Sbjct 296 GATTTTCCCGCTCCGGAGAGATTCGGATCTGGATTTTTCG-AGCTGATTTGTAGGAATTT 354
Query 329 CTCGTT 334
||||||
Sbjct 355 CTCGTT 360
> AT2G20110.2 | Symbols: | Tesmin/TSO1-like CXC domain-containing
protein | chr2:8684496-8687218 FORWARD LENGTH=2085
Length=2085
Score = 736 bits (398), Expect = 0.0
Identities = 731/885 (83%), Gaps = 50/885 (6%)
Strand=Plus/Plus
Query 649 ATCCTTAACG-CGCCAATCAGGCATCCAAAACCGGAATCTCCAAAAGCTAGAGGTCCAAG 707
|||||| ||| | ||||| || ||||| | || |||||||| || | || | |||||
Sbjct 259 ATCCTTCACGTC-CCAATTAGACATCCGAGGCCCGAATCTCC-GAA-TTCGATG-CCAAG 314
Query 708 GCCTATTGTGGAAGGTAGAGATGGAACTCCTCAGAAGAAGAAGCAGTGTAACTGTAAACA 767
|||| || |||| |||||||||||| |||||||||||||| |||||||||||||||||
Sbjct 315 GCCTGCTGGGGAAACTAGAGATGGAACCCCTCAGAAGAAGAAACAGTGTAACTGTAAACA 374
Query 768 CTCACGCTGCTTGAAACTGTACTGTGAATGCTTTGCATCCGGAACATATTGTGACGGTTG 827
|||||| |||||||| ||||| |||||||| |||||||| || || || ||||| |||||
Sbjct 375 CTCACGGTGCTTGAAGCTGTATTGTGAATGTTTTGCATCTGGCACTTACTGTGATGGTTG 434
Query 828 TAACTGTGTAAATTGTTTCAACAATGTTGATAATGAACCTGCAAGACGAGAAGCTGTGGA 887
||||||||||||||||||||||| ||||| ||||||||||| |||||| |||| || ||
Sbjct 435 CAACTGTGTAAATTGTTTCAACAACGTTGAAAATGAACCTGCGAGACGACAAGCGGTTGA 494
Query 888 AGCAACTCTGGAGCGGAATCC-ATTTGCGTTCAGGCCTAAA-ATTGCTAGC-AGTCCACA 944
| |||||||||| |||||||| | |||||||||||| ||| |||||| || ||||||||
Sbjct 495 ATCAACTCTGGAACGGAATCCGA-ATGCGTTCAGGCC-AAAGATTGCT-GCTAGTCCACA 551
Query 945 TGGTGTG-CGGGATAAAAGGGAGGACATTGGTGAAGTTGTGTTGTTAGGGAAACATAACA 1003
||||| | || ||||| |||||||| ||||||| |||||| |||||| || ||| ||||
Sbjct 552 TGGTG-GACGAGATAACAGGGAGGAGGTTGGTGACGTTGTGATGTTAGCGAGACACAACA 610
Query 1004 AAGGATGCCACTGCAAGAAATCGGGATGCCTTAAGAAGTATTGTGAGTGCTTTCAAGCAA 1063
|||| || |||||||||||||| ||||| |||||||||||||||||||||||||||||||
Sbjct 611 AAGGCTGTCACTGCAAGAAATCCGGATGTCTTAAGAAGTATTGTGAGTGCTTTCAAGCAA 670
Query 1064 ACATTCTTTGTTCTGAGAACTGCAAATGCTTGGATTGCAAAAACTTTGAAGGAAGTGAAG 1123
|||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
Sbjct 671 ACATTCTTTGTTCTGAGAACTGCAAATGCTTGGATTGCAAAAACTTTGAAGGGAGTGAAG 730
Query 1124 AGAGACAAGCTCTGTTTCACGGTGAACATGCCAACCACATGGCTTATCTTCAACAAGCAG 1183
|||||| |||| ||||||||||||||| || || || |||||||||||||||| | |
Sbjct 731 TTAGACAATCTCTTTTTCACGGTGAACATTCCCACAACTTGGCTTATCTTCAACA--C-G 787
Query 1184 CAAATGCAGCCATTACTGGAGCTGTTGGTTCCTCCGGCTTTGC-TCCTTCTCCAGCACCT 1242
||||||| ||||| || |||||| |||||||||| |||||||| || | ||||| | |||
Sbjct 788 CAAATGCTGCCATAACCGGAGCTATTGGTTCCTCTGGCTTTGCATC-TGCTCCACCTCCT 846
Query 1243 AAGAGAAGGAAAGGTCAAGAGATTTTGTTCAACCAAGCAACCAAAGATTCATCTAGACTT 1302
||||||||||||||||| |||||||| |||||||||| ||||||||||||||| | | |
Sbjct 847 AAGAGAAGGAAAGGTCAGGAGATTTTCTTCAACCAAGGAACCAAAGATTCATCCACTCAT 906
Query 1303 -GGCCAGTTTCCACGGGTAAATAGTGGAAGAGCTAGTGGACCAACCTCAGGCTCATCTCC 1361
||| | ||| || ||||| |||||| | ||| | | || | ||
Sbjct 907 AGGCTTGG--CCA-GGC-AAATAACGGAAGA---A-----CCA-C---A---TC-T-TC- 944
Query 1362 GTCAC-CTGTTTCTCGTGCTGGTGGCAATGCATCGTCAG-CTCCATC-AAAGTTTGTATA 1418
| || ||| |||||||||||| |||||||| |||| || | ||||| |||||| |||||
Sbjct 945 G-CAAACTGGTTCTCGTGCTGGAGGCAATGCGTCGTTAGGC-CCATCCAAAGTT-GTATA 1001
Query 1419 TAGGTCCCTTTTAGCAGATATAATCCAACCGCATG-ATGTTAGAGCACTTTGCTCTGTTT 1477
|| ||| ||||| ||| | ||||| |||| ||| |||| | |||||| || ||||||
Sbjct 1002 TAAGTCTCTTTTGGCAAACATAATTAAACCA-ATGGATGTGAAAGCACTATGTTCTGTTC 1060
Query 1478 TAGTCGCTGTAGCCGGAGAAGCAGCAAAGACATCAACAGATAAAA 1522
| || |||||||| || ||||| |||||||||| |||||| ||||
Sbjct 1061 TGGTTGCTGTAGCTGGGGAAGCTGCAAAGACATTAACAGA-AAAA 1104
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 130584552629
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5