BLASTN 2.2.26+ Reference: Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000), "A greedy algorithm for aligning DNA sequences", J Comput Biol 2000; 7(1-2):203-14. Database: TAIR10_cdna_20110103_representative_gene_model_updated 33,602 sequences; 51,074,197 total letters Query= Ahg493523 Length=1175 Score E Sequences producing significant alignments: (Bits) Value AT4G13890.1 | Symbols: SHM5, EDA36, EDA37 | Pyridoxal phosphate... 1711 0.0 AT4G13930.1 | Symbols: SHM4 | serine hydroxymethyltransferase 4... 1358 0.0 > AT4G13890.1 | Symbols: SHM5, EDA36, EDA37 | Pyridoxal phosphate (PLP)-dependent transferases superfamily protein | chr4:8031972-8033723 REVERSE LENGTH=1592 Length=1592 Score = 1711 bits (926), Expect = 0.0 Identities = 1094/1177 (93%), Gaps = 4/1177 (0%) Strand=Plus/Plus Query 1 AGAGATGGAATCAGTCTCTTCTTGGGGAAACACGCCTCTTGTCTCCGTCGATCCCGAGAT 60 |||||||||| |||||| ||||||||||||||||| ||||| || ||||||||| ||||| Sbjct 2 AGAGATGGAACCAGTCTATTCTTGGGGAAACACGCATCTTGACTTCGTCGATCCGGAGAT 61 Query 61 CCACGACCTAATCGAGAAGGAGAAACATCGGCAATGTCGAGGAATCGAACTCATAGCCGC 120 | |||||||||||||||||||||||||||| |||||||||||||||||||||||||| || Sbjct 62 CTACGACCTAATCGAGAAGGAGAAACATCGTCAATGTCGAGGAATCGAACTCATAGCAGC 121 Query 121 CGAGAATTTCACTTCCTTGGCTGTCATGGAAGCTCTTGGAAG-TGCCTTAACCAACAAAT 179 |||||||||||||||| |||| |||||||||||||| ||||| || |||||||||||||| Sbjct 122 CGAGAATTTCACTTCCGTGGCCGTCATGGAAGCTCTCGGAAGTTG-CTTAACCAACAAAT 180 Query 180 ACTCGGAAGGTATGCCTGGTAATCGTTACTACGGAGGTACCGAGTTCATCGATGAGATCG 239 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| | Sbjct 181 ACTCGGAAGGTATGCCTGGTAATCGTTACTACGGAGGTACCGAGTTCATCGATGAGATAG 240 Query 240 AGAGTCTCTGCCGCTCACGCGCTCTCGAGGCTTTCCATTGTGATCCAGAGAAATGGGGCG 299 |||||||||||||||||||| ||||||| || |||||||| |||||||||||||||||| Sbjct 241 AGAGTCTCTGCCGCTCACGCTCTCTCGAAGCCTTCCATTGCAATCCAGAGAAATGGGGCG 300 Query 300 TTAATGTTCAGCCTTACTCTGGTTCTCCTGCAAACTTCGCCGCCTACACGGCCTTGCTCC 359 |||| |||||||||||||||||||| ||||| || ||||| |||||||| |||||||||| Sbjct 301 TTAACGTTCAGCCTTACTCTGGTTCCCCTGCGAATTTCGCAGCCTACACCGCCTTGCTCC 360 Query 360 AGCCTCATGATCGTATCATGAGGCTTGATCTGCCTTCAGGTGGTCATCTAACGCATGGTT 419 |||||||||||||||||||| |||||||| |||| |||||||||||| |||||||||||| Sbjct 361 AGCCTCATGATCGTATCATGGGGCTTGATTTGCCATCAGGTGGTCATATAACGCATGGTT 420 Query 420 ACTATACCTCTGGTGGGAAGAATATATCCGCGACCTCGATCTACTTCGAAAACCTTCTGT 479 |||||| ||||||||||||||| || |||||||||||||||||||| |||||||||| | Sbjct 421 ACTATAGCTCTGGTGGGAAGAACATCTCCGCGACCTCGATCTACTTTGAAAACCTTCCCT 480 Query 480 ACAAGGTGGATTCCACCACTGGTTACATTGATTACGACAAGCTTGAGGAGAAAGCAATGG 539 ||||||||||||||| ||||||||||||||||||||||| |||||||||||||| |||| Sbjct 481 ACAAGGTGGATTCCAAGACTGGTTACATTGATTACGACAAACTTGAGGAGAAAGCTATGG 540 Query 540 ATTTTAGGCCTAAGTTAATCATCTGTGGAGGCACTTCTTACCCTAGGGAATGGGATTACG 599 ||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||| Sbjct 541 ATTTTAGGCCTAAGTTAATTATCTGTGGAGGCACTTCTTACCCTAGGGAATGGGATTACG 600 Query 600 CGAGGTTCAAAGTTGTCGCGGATAAGGTTGGAGCTCTTTTGCTTTGTGATATGGCTCACA 659 |||| |||| | |||||| || |||||||||||| ||||||| |||||||||||||| | Sbjct 601 CGAGATTCAGGGCTGTCGCCGACAAGGTTGGAGCTTTTTTGCTCTGTGATATGGCTCATA 660 Query 660 TCAGTGGTCTCGTTGCTGCTCAGGAAGCTGCAGACCCGTTCGAGTACTGTGACGTTGTGA 719 ||||| |||||||||||||||||||| ||||||||||||||||||||||||||||||| Sbjct 661 ACAGTGCCCTCGTTGCTGCTCAGGAAGCCGCAGACCCGTTCGAGTACTGTGACGTTGTGA 720 Query 720 CAACCTCAACTCACAAGAGTTTGAGAGGTCCAAGGGCTGGTATGATCTTTTATAGAAAGG 779 |||| |||||||||||||| |||||||||||||||||||||||||||||||||||||||| Sbjct 721 CAACGTCAACTCACAAGAGCTTGAGAGGTCCAAGGGCTGGTATGATCTTTTATAGAAAGG 780 Query 780 GCCCAAAACCAGCCAAGAAGGGCCAACCCGAGGGTGAAGTCTACGATTTTGACaaaaaaa 839 | ||||||||||||||||||||||||||||||||||||||||||||||| ||| ||||| Sbjct 781 GACCAAAACCAGCCAAGAAGGGCCAACCCGAGGGTGAAGTCTACGATTTCGACGCAAAAA 840 Query 840 TCAACTTTGCGGTGTTCCCTGCGCTTCAAAGTGGTCCTCACAATAACAAGATTGGTGCTC 899 |||||| |||||| |||||||||||||||||||||||||||||||||||||||||||||| Sbjct 841 TCAACTCTGCGGTATTCCCTGCGCTTCAAAGTGGTCCTCACAATAACAAGATTGGTGCTC 900 Query 900 TAGCTGTTGCGTTGAAGCAGGCTATGGCTCCTGGGTTCAAGGTCTACGCAAAACAGGTGA 959 ||||||||||||||||||||| |||||||||| | ||||| || || ||||||||||||| Sbjct 901 TAGCTGTTGCGTTGAAGCAGGTTATGGCTCCTAGCTTCAAAGTTTATGCAAAACAGGTGA 960 Query 960 AAGCCAATGCTGCTTGCCTTGGAAACTACCTAATGGGA-AAGGGATATTGTATTGTAACC 1018 |||| |||||||||||||||| || ||||||||| | ||||||||| | |||||||| Sbjct 961 AAGCTAATGCTGCTTGCCTTGCAAGCTACCTAATAA-ATAAGGGATATACTCTTGTAACC 1019 Query 1019 GGTGGAACTGATAACCACCTTGTTCTTTGGGATCTTCGCCCTCTTGGATTGACCGGTAAC 1078 | ||||||||||||||||||| |||||||||||||||||||||||||||||||||||||| Sbjct 1020 GATGGAACTGATAACCACCTTATTCTTTGGGATCTTCGCCCTCTTGGATTGACCGGTAAC 1079 Query 1079 AAGGTTGAGAAGGTTAGTGAATTGGGCTACATTACTTTGAACAGGAATGCGGTATTCGGA 1138 ||||||||||||||| |||||||| ||||||||||||||||||||||||||||||||||| Sbjct 1080 AAGGTTGAGAAGGTTTGTGAATTGTGCTACATTACTTTGAACAGGAATGCGGTATTCGGA 1139 Query 1139 GACACTAGTTTTCTTGCTCCTGGAGGTGTAAGAATCG 1175 |||||||||||||||||||| |||||||||||||||| Sbjct 1140 GACACTAGTTTTCTTGCTCCCGGAGGTGTAAGAATCG 1176 > AT4G13930.1 | Symbols: SHM4 | serine hydroxymethyltransferase 4 | chr4:8047692-8050106 REVERSE LENGTH=1822 Length=1822 Score = 1358 bits (735), Expect = 0.0 Identities = 1037/1183 (88%), Gaps = 20/1183 (2%) Strand=Plus/Plus Query 3 AGATGGAATCAGTCTCTTCTTGGGGAAACACGC-CTCTTGTCTCCGTCGATCCCGAGATC 61 |||||||| |||||||||| ||||| ||||| | |||| |||||||| ||||| |||||| Sbjct 84 AGATGGAACCAGTCTCTTCATGGGGTAACAC-CTCTCTCGTCTCCGTAGATCCAGAGATC 142 Query 62 CACGACCTAATCGAGAAGGAGAAACATCGGCAATGTCGAGGAATCGAACTCATAGCCGCC 121 ||||||||||||||||||||||||| |||||||||||||||||||||||| || || || Sbjct 143 CACGACCTAATCGAGAAGGAGAAACGTCGGCAATGTCGAGGAATCGAACTTATCGCTTCC 202 Query 122 GAGAATTTCACTTCCTTGGCTGTCATGGAAGCTCTTGGAAGTGCCTTAACCAACAAATAC 181 ||||||||||||||||| || ||||| |||||||| |||||||||||||||||||||||| Sbjct 203 GAGAATTTCACTTCCTTCGCCGTCATCGAAGCTCTCGGAAGTGCCTTAACCAACAAATAC 262 Query 182 TCGGAAGGTATGCCTGGTAATCGTTACTACGGAGGTACCGAGTTCATCGATGAGATCGAG 241 || |||||||| |||||||||||||| |||||||||| ||| |||||||||||||||||| Sbjct 263 TCCGAAGGTATTCCTGGTAATCGTTATTACGGAGGTAACGAATTCATCGATGAGATCGAG 322 Query 242 AGTCTCTGCCGCTCACGCGCTCTCGAGGCTTTCCATTGTGATCCAG-AGAAATGGGGCGT 300 | ||| ||||| ||| | |||||||| ||||||||||||||||||| || |||||||| Sbjct 323 AATCTTTGCCGTTCAAGAGCTCTCGAAGCTTTCCATTGTGATCCAGCAG-CGTGGGGCGT 381 Query 301 TAATGTTCAGCCTTACTCTGGTTCTCCTGCAAACTTCGCCGCCTACACGGCCTTGCTCCA 360 |||||||||||| || ||||| ||||| ||||| ||||| || |||||||| ||||| || Sbjct 382 TAATGTTCAGCCGTATTCTGGATCTCCGGCAAATTTCGCTGCTTACACGGCTTTGCTTCA 441 Query 361 GCCTCATGATCGTATCATGAGGCTTGATCTGCCTTCAGGTGGTCATCTAACGCATGGTTA 420 |||||| |||||||||||| |||||||| ||||||||||||||||| | || |||||||| Sbjct 442 GCCTCACGATCGTATCATGGGGCTTGATTTGCCTTCAGGTGGTCATTTGACTCATGGTTA 501 Query 421 CTATACCTCTGGTGGGAAGAATATATCCGCGACCTCGATCTACTTCGAAAACCTTCTGTA 480 |||||| |||||||| ||||| || || ||||| ||||||||||| || | |||| ||| Sbjct 502 CTATACATCTGGTGGTAAGAAGATCTCTGCGACTTCGATCTACTTTGAGAGTCTTCCGTA 561 Query 481 CAAGGTGGATTCCACCACTGGTTACATTGATTACGACAAGCTTGAGGAGAAAGCAATGGA 540 |||||| ||| |||||||||||||||||||||||| |||||||| ||||| || |||| Sbjct 562 TAAGGTGAATTTCACCACTGGTTACATTGATTACGATAAGCTTGAAGAGAAGGCGTTGGA 621 Query 541 TTTTAGGCCTAAGTTAATCATCTGTGGAGGCACTTCTTACCCTAGGGAATGGGATTACGC 600 ||| ||||||||||| | |||||||| || | | | || |||||||| ||||||||||| Sbjct 622 TTTCAGGCCTAAGTTGCTTATCTGTGGTGGTAGTGCGTATCCTAGGGATTGGGATTACGC 681 Query 601 GAGGTTCAAAGTTGTCGCGGATAAGGTTGGAGCTCTTTTGCTTTGTGATATGGCTCACAT 660 || || | || | | || ||||||||||||||||||||||||||||| ||||||||||| Sbjct 682 TAGATTTAGAGCTATTGCTGATAAGGTTGGAGCTCTTTTGCTTTGTGACATGGCTCACAT 741 Query 661 CAGTGGTCTCGTTGCTGCTCAGGAAGCTGCAGACCCGTTCGAGTACTGTGACGTTGTGAC 720 ||||||||||||||||||||||||||||||| |||| || |||||||||||||||||||| Sbjct 742 CAGTGGTCTCGTTGCTGCTCAGGAAGCTGCAAACCCATTTGAGTACTGTGACGTTGTGAC 801 Query 721 AACCTCAACTCACAAGAGTTTGAGAGGTCCAAGGGCTGGTATGATCTTT-TATAGAAAGG 779 |||| |||| |||||||||||||| |||||||||||||||||||| ||| || || |||| Sbjct 802 AACCACAACCCACAAGAGTTTGAGGGGTCCAAGGGCTGGTATGAT-TTTCTACAGGAAGG 860 Query 780 GCCCAAAACCAGCCAA-GAAGGGCCAACCCGAGGGTGAAGTCTACGATTTTGACaaaaaa 838 | ||||||||| |||| |||||| ||||||||||||| |||||| |||||||| | ||| Sbjct 861 GTCCAAAACCA-CCAAAGAAGGGTCAACCCGAGGGTGCAGTCTATGATTTTGAGGACAAA 919 Query 839 aTCAACTTTGCGGTGTTCCCTGCGCTTCAAAGTGGTCCTCACAATAACAAGATTGGTGCT 898 ||||||||||| || ||||||||||||||| |||||||||||||| || ||||||||||| Sbjct 920 ATCAACTTTGCTGTATTCCCTGCGCTTCAAGGTGGTCCTCACAATCACCAGATTGGTGCT 979 Query 899 CTAGCTGTTGCGTTGAAGCAGGCTA-TGGCTCCTGGGTTCAAGGTCTACGCAAAACAGGT 957 ||||||||||||||||||||||||| | |||||||||||||||||||||||||||| || Sbjct 980 CTAGCTGTTGCGTTGAAGCAGGCTAATA-CTCCTGGGTTCAAGGTCTACGCAAAACAAGT 1038 Query 958 GAAAGCCAATGCTGCTTGCC-TTGGAAACTACCTAATGGGAAAGGGATATT-GTATTGTA 1015 |||||||||||||| ||||| ||||||||||||||||| | ||||| || | ||||| Sbjct 1039 GAAAGCCAATGCTG-TTGCCCTTGGAAACTACCTAATGAGCAAGGGTTACCAG-ATTGTG 1096 Query 1016 ACCGGTGGAACTGATAACCACCTTGTTCTTTGGGATCTTCGCCCTCTTGGATTGACCGGT 1075 ||| ||||||||| |||||||||||||| ||||||||||||||||||||||||||||| Sbjct 1097 ACCAATGGAACTGAGAACCACCTTGTTCTCTGGGATCTTCGCCCTCTTGGATTGACCGGA 1156 Query 1076 AACAAGGTTGAGAAGGT-TAGTGAAT-TGGGCTA-CATTACTTTGAACAGGAATGCGGTA 1132 ||||||||||||||| | | | || | || || | |||||| ||||||| ||| || ||| Sbjct 1157 AACAAGGTTGAGAAGCTCT-GCGA-TCTGTGC-AGCATTACATTGAACAAGAACGCAGTA 1213 Query 1133 TTCGGAGACACTAGTTTTCTTGCTCCTGGAGGTGTAAGAATCG 1175 || ||||||| |||| |||||||||||||||||||||||||| Sbjct 1214 TTTGGAGACAGTAGTGCTCTTGCTCCTGGAGGTGTAAGAATCG 1256 Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 57680426205 Database: TAIR10_cdna_20110103_representative_gene_model_updated Posted date: Sep 25, 2014 6:13 PM Number of letters in database: 51,074,197 Number of sequences in database: 33,602 Matrix: blastn matrix 1 -2 Gap Penalties: Existence: 0, Extension: 2.5