BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg495082
Length=996
Score E
Sequences producing significant alignments: (Bits) Value
AT5G50240.1 | Symbols: PIMT2, AtPIMT2 | protein-l-isoaspartate ... 1378 0.0
AT5G50250.1 | Symbols: CP31B | chloroplast RNA-binding protein ... 113 3e-24
> AT5G50240.1 | Symbols: PIMT2, AtPIMT2 | protein-l-isoaspartate
methyltransferase 2 | chr5:20451228-20452569 FORWARD LENGTH=1061
Length=1061
Score = 1378 bits (746), Expect = 0.0
Identities = 897/967 (93%), Gaps = 21/967 (2%)
Strand=Plus/Plus
Query 49 GAATaaaa-aaaaTGAGAGCTCAGGTTTTATTATGCCCAACAATAACAGCCTATGGTCGC 107
||| |||| |||||||||||||||||||||||||| |||||||||||||| ||||||||
Sbjct 97 GAA-AAAAGAAAATGAGAGCTCAGGTTTTATTATGTCCAACAATAACAGCTTATGGTCGT 155
Query 108 CTCTATTGTGCGCCACGTAGACTTCACCGCTACAA-T--TCATCATCATCATCTCAGTTT 164
||||||||||||||||||||||||||||||||||| | |||||||||||||||||||||
Sbjct 156 CTCTATTGTGCGCCACGTAGACTTCACCGCTACAATTCATCATCATCATCATCTCAGTTT 215
Query 165 CTGGATCTCAGTCTCTCTCGCTTCTCCGGTCG-TCTCTTCTTCCACAT-G-G-------A 214
||| |||||| ||||||||||||||||||| | ||||||||||||||| | | |
Sbjct 216 CTGAATCTCAATCTCTCTCGCTTCTCCGGT-GCTCTCTTCTTCCACATGGAGCAATTCCA 274
Query 215 GAGTGGAACTAGCTCGAGTGGCAAGAAATCAATGGTGGAAAATCTGAAGCGGTACGGTGT 274
|||||||||| ||||||| ||||||| | |||||||||||| ||||||||||||||||
Sbjct 275 GAGTGGAACTGGCTCGAGCGGCAAGAGAGGGATGGTGGAAAATTTGAAGCGGTACGGTGT 334
Query 275 GATATCATCGAAGAGAGTAGCTCAAGTTATGGAAGCTTTAGATAGAGGTCTCTTTGTACC 334
|||||| ||||| |||||||||||||||||||| ||||||||||||||||||||||||||
Sbjct 335 GATATCGTCGAAAAGAGTAGCTCAAGTTATGGAGGCTTTAGATAGAGGTCTCTTTGTACC 394
Query 335 AGTTGGATCTTCAGCTTATGTTGATACTCCTGTACCTATAGGTTATAATGCTACCATTTC 394
|||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 395 AGTTGGTTCTTCAGCTTATGTTGATACTCCTGTACCTATAGGTTATAATGCTACCATTTC 454
Query 395 TGCTCCGCATATGCATGCTACTTGCTTGCAGCTTTTGGAAGATAAGCTCAAGCCCGGGAT 454
||||||||||||||||||||||||||||||||| ||||||||||||||| | ||||||||
Sbjct 455 TGCTCCGCATATGCATGCTACTTGCTTGCAGCTCTTGGAAGATAAGCTCCACCCCGGGAT 514
Query 455 GCGTGCTTTGGATGTTGGTTCAGGAACCGGGTACTTGACTGGCTGTTTTGCTTTGATGGT 514
||| ||||| ||||| || |||||||||||||||||||||||||||||||||||||||||
Sbjct 515 GCGCGCTTTAGATGTCGGCTCAGGAACCGGGTACTTGACTGGCTGTTTTGCTTTGATGGT 574
Query 515 TGGAGCTGAGGGACGCGTTGTTGGTGTGGATCATATCCCTGAATTGGTTGATATGTCTAT 574
||||||||||||||| |||||||| |||||||||||||||||||||||||||||||||||
Sbjct 575 TGGAGCTGAGGGACGTGTTGTTGGCGTGGATCATATCCCTGAATTGGTTGATATGTCTAT 634
Query 575 CAAGAATATCGAAAAGAGTGTCGCTGCTTCCTTGCTTAAGAAAGGATCTCTCTCCCTACA 634
||||||||||||||||||||| ||||||||||| ||||||||||||||||||||| ||||
Sbjct 635 CAAGAATATCGAAAAGAGTGTTGCTGCTTCCTTTCTTAAGAAAGGATCTCTCTCCTTACA 694
Query 635 TGTTGGTGATGGAAGAAAAGGTTGGCAAGAGTTTGCACCATATGATGCGATACACGTAGG 694
|||||| |||||||||||||||||||||||||||||||| |||||||| |||||||||||
Sbjct 695 TGTTGGGGATGGAAGAAAAGGTTGGCAAGAGTTTGCACCGTATGATGCAATACACGTAGG 754
Query 695 AGCAGCGGCATCAGAAATCCCGCAGCCTCTTTTGGATCAGCTAAAACCCGGTGGGCGAAT 754
||||||||||||||||||||| ||||| |||||||||||||||||||||||||| |||||
Sbjct 755 AGCAGCGGCATCAGAAATCCCTCAGCCCCTTTTGGATCAGCTAAAACCCGGTGGACGAAT 814
Query 755 GGTGATTCCACTAGGAACCTATTTTCAAGAGCTCAAGGTAATCGACAAAAACAAAGATGG 814
|||||||||||||||||||||||||||||||||||||||||||||||||||| | |||||
Sbjct 815 GGTGATTCCACTAGGAACCTATTTTCAAGAGCTCAAGGTAATCGACAAAAACGAGGATGG 874
Query 815 TTCCATCAAGGTGCATACCGAAACTTCGGTCAGGTATGTGCCCCTCACTAGCCGCGACGA 874
||| |||||||| |||||||||||||| ||| |||||||||||||||||||||| | |||
Sbjct 875 TTCAATCAAGGTACATACCGAAACTTCAGTCCGGTATGTGCCCCTCACTAGCCGTGTCGA 934
Query 875 ACAGTTAGGAGGATTTTGACATCAACGA-A--GAGAAAACTCTACATACCACGCCTTAGT 931
||||||||||||||||||||| |||| | | ||||||||||||||| ||||| ||||||
Sbjct 935 ACAGTTAGGAGGATTTTGACAACAACAACAAAGAGAAAACTCTACATCCCACGTCTTAGT 994
Query 932 GTACATATAGA-GGTTGTG-AATGTTCTGTAATATGTTTTGATGGGTAATAAAGAAAATC 989
||||||||| | ||||||| |||||| ||||||||||||||||||||||||||||||||
Sbjct 995 GTACATATAAAAGGTTGTGTAATGTTTTGTAATATGTTTTGATGGGTAATAAAGAAAATA 1054
Query 990 TGTTTAC 996
|||||||
Sbjct 1055 TGTTTAC 1061
Score = 52.8 bits (28), Expect = 6e-06
Identities = 28/28 (100%), Gaps = 0/28 (0%)
Strand=Plus/Plus
Query 25 AACAGATCATCAGAGAAGAAACTAGAAT 52
||||||||||||||||||||||||||||
Sbjct 49 AACAGATCATCAGAGAAGAAACTAGAAT 76
> AT5G50250.1 | Symbols: CP31B | chloroplast RNA-binding protein
31B | chr5:20452487-20454013 REVERSE LENGTH=1108
Length=1108
Score = 113 bits (61), Expect = 3e-24
Identities = 76/83 (92%), Gaps = 2/83 (2%)
Strand=Plus/Minus
Query 916 CATACCACGCCTTAGTGTACATAT-AGAGGTTGTG-AATGTTCTGTAATATGTTTTGATG 973
||| ||||| |||||||||||||| | |||||||| |||||| |||||||||||||||||
Sbjct 1108 CATCCCACGTCTTAGTGTACATATAAAAGGTTGTGTAATGTTTTGTAATATGTTTTGATG 1049
Query 974 GGTAATAAAGAAAATCTGTTTAC 996
||||||||||||||| |||||||
Sbjct 1048 GGTAATAAAGAAAATATGTTTAC 1026
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 48777356737
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5