BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg495109
Length=1169
Score E
Sequences producing significant alignments: (Bits) Value
AT5G50430.1 | Symbols: UBC33 | ubiquitin-conjugating enzyme 33 ... 1615 0.0
AT1G17280.1 | Symbols: UBC34 | ubiquitin-conjugating enzyme 34 ... 765 0.0
> AT5G50430.1 | Symbols: UBC33 | ubiquitin-conjugating enzyme 33
| chr5:20534173-20537117 REVERSE LENGTH=1216
Length=1216
Score = 1615 bits (874), Expect = 0.0
Identities = 1068/1157 (92%), Gaps = 31/1157 (3%)
Strand=Plus/Plus
Query 35 CGAATGATTTGATCATC-ATCATCGACGAGAA-ACACA--GAG--AGAGAATCTTAAATT 88
||||||||||||||||| |||| ||| || || ||||| ||| ||||||||| |||||
Sbjct 67 CGAATGATTTGATCATCAATCAACGA-GA-AACACACACGGAGAAAGAGAATCTCAAATT 124
Query 89 CGCTCTAGCTCCTGATCGGTTTCGATTTGTCACAATTC-TTCCTTGGATCTGCTCTTACC 147
|||| |||||||||||| || |||||| ||||||||| |||||||||||||||||||||
Sbjct 125 AGCTCCAGCTCCTGATCGATTCCGATTT-TCACAATTCTTTCCTTGGATCTGCTCTTACC 183
Query 148 TAGTTACCA-ATCACTTGGCTGCCAttttt-atttttttttCTGCAGT-TTACAAT-TCG 203
| || || | |||||| ||| ||||| | ||| | | || || | |||| ||
Sbjct 184 TTGTCACGATTTCACTTCCCTG-TGTTTTTGA-TTTATACT-TG--GTCATCCAATAACG 238
Query 204 TTGTTTTGAT-TAACTGGAACTACAATTAATTGGATCTCCCTGAAGCATTTAGATGGCAG 262
|||||| ||||||||||||| || |||||| ||||||||||||||||||||||||
Sbjct 239 AAACTTTGATCAAACTGGAACTACAGTTTATTGGAACTCCCTGAAGCATTTAGATGGCAG 298
Query 263 AAAAAGCTTGTATAAAGCGTCTTCAAAAAGAATACAGAGCACTTTGCAAGGAACCAGTCT 322
|||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
Sbjct 299 AAAAAGCTTGTATAAAGCGTCTTCAAAAAGAATACAGAGCGCTTTGCAAGGAACCAGTCT 358
Query 323 CGCATGTTGTTGCTCGGCCTTCCCCAAATGACATTCTTGAGTGGCATTATGTGTTGGAAG 382
|||||||||||||||| ||||||||||||||||||||||| |||||||||||||||||||
Sbjct 359 CGCATGTTGTTGCTCGTCCTTCCCCAAATGACATTCTTGAATGGCATTATGTGTTGGAAG 418
Query 383 GCAGTGAGGGAACACCTTTTGCAGGTGGATTTTACTATGGGAAGATCAAGTTCCCTCCAG 442
||||||||||||| |||||||||||||||||||||||||| |||||||||||||||||||
Sbjct 419 GCAGTGAGGGAACGCCTTTTGCAGGTGGATTTTACTATGGAAAGATCAAGTTCCCTCCAG 478
Query 443 AATATCCTTACAAGCCACCTGGAATAACAATGACCACACCAAATGGTCGATTTGTGACAC 502
||||||||||||||||||||||||| |||||||||||||||||||||||||||||||| |
Sbjct 479 AATATCCTTACAAGCCACCTGGAATCACAATGACCACACCAAATGGTCGATTTGTGACGC 538
Query 503 AAAAGAAAATCTGTTTGTCTATGAGTGACTTTCATCCAGAAAGCTGGAATCCAATGTGGT 562
||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 539 AAAAGAAAATCTGCTTGTCTATGAGTGACTTTCATCCAGAAAGCTGGAATCCAATGTGGT 598
Query 563 CAGTGTCAAGCATACTTACAGGACTTCTCTCATTTATGATGGACAACAGCCCCACAACGG 622
||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
Sbjct 599 CAGTGTCAAGCATACTTACAGGACTTCTCTCATTTATGATGGATAACAGCCCCACAACGG 658
Query 623 GAAGTGTAAACACTAGTGTAGCCGATAAACAACAGTTGGCGAAGTCATCTCTTGCTTTTA 682
||||||||||||||||||||||||| ||||||| |||||||||||||||||||||||| |
Sbjct 659 GAAGTGTAAACACTAGTGTAGCCGAGAAACAACGGTTGGCGAAGTCATCTCTTGCTTTCA 718
Query 683 ATTGTAAAAATGCAACATTCCGGAAACTATTTCCCGAGTATGTAGAGAAGTACAGCCAGC 742
||||||||| || |||||||||||||||||||||||||||||| ||||||||||||||||
Sbjct 719 ATTGTAAAAGTGTAACATTCCGGAAACTATTTCCCGAGTATGTCGAGAAGTACAGCCAGC 778
Query 743 AACAAGTAGCTGAAGAGGAAGCAGCCACTCAACAGACAACA-C--CTGAG-----G-ATT 793
|||||||||||||||| |||||||||||||||||||||||| | ||||| | |||
Sbjct 779 AACAAGTAGCTGAAGAAGAAGCAGCCACTCAACAGACAACAACATCTGAGAATCAGGATT 838
Query 794 CTCCTCAGGAGGAAAATGCGAAAGTCGAATCAGAGAAAAGCATTGGTCTTAAAAAGGACA 853
||||||| |||| ||||||||||||||||||||||||||| |||||||||||||||| |
Sbjct 839 TTCCTCAGAAGGACAATGCGAAAGTCGAATCAGAGAAAAGCGTTGGTCTTAAAAAGGAAA 898
Query 854 GTATTCAAGAAGTGGGATTGAAAGAGAGGAGAAAGAACAAGAAAGAAGCATTACCGGGTT 913
||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||
Sbjct 899 GTATTCAAGAAGTGGGATTGAAAGAGAGGAGAAGGAACAAGAAAGAAGCATTACCGGGTT 958
Query 914 GGATAGTATTGCTGCTAGTATCGATTGTCGGTGTTGTAATGGCGTTGCCTTTGCTTCAAC 973
||||||||||| |||||||||||||||||||||||||||||||||||||||||||||| |
Sbjct 959 GGATAGTATTGTTGCTAGTATCGATTGTCGGTGTTGTAATGGCGTTGCCTTTGCTTCAGC 1018
Query 974 TGTGATTTAGATGCAAATGCAACGCAACACAACATGACTGCAAATCAATCTGGTAAAATC 1033
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1019 TGTGATTTAGATGCAAATGCAACGCAACACAACATGACTGCAAATCAATCTGGTAAAATC 1078
Query 1034 TGTTTATCATCGTCTCTCACTCTCAGTACTAAGTAGTGTCTTTTGTTAT-CCCCCTTCTC 1092
|||||||||| |||||||| ||||||||||||||||||||||||||| | |||||| |||
Sbjct 1079 TGTTTATCATTGTCTCTCAGTCTCAGTACTAAGTAGTGTCTTTTGTTCTTCCCCCT-CTC 1137
Query 1093 TTGAAAAGAAAACAGATACATTGAGTTTGGTATGGCATTTGAAATTACATATAAATATAA 1152
||||||||||||||||||||||||||||||||||||||||||| |||| |||||||||||
Sbjct 1138 TTGAAAAGAAAACAGATACATTGAGTTTGGTATGGCATTTGAAGTTACGTATAAATATAA 1197
Query 1153 ATGTTAGCTGAATTTGA 1169
||||||||| |||||||
Sbjct 1198 ATGTTAGCTTAATTTGA 1214
> AT1G17280.1 | Symbols: UBC34 | ubiquitin-conjugating enzyme 34
| chr1:5916871-5920089 REVERSE LENGTH=1135
Length=1135
Score = 765 bits (414), Expect = 0.0
Identities = 637/743 (86%), Gaps = 21/743 (3%)
Strand=Plus/Plus
Query 253 TAGATGGCAGAAAAAGCTTGTATAAAGCGTCTTCAAAAAGAATACAGAGCACTTTGCAAG 312
|||||||||||||| || |||||||| ||||||||||| ||||| |||||||||||||||
Sbjct 144 TAGATGGCAGAAAAGGCCTGTATAAAACGTCTTCAAAAGGAATATAGAGCACTTTGCAAG 203
Query 313 GAACCAGTCTCGCATGTTGTTGCTCGGCCTTCCCCAAATGACATTCTTGAGTGGCATTAT 372
||||||||||| |||||||||||||| |||||||||||||||||||| ||||||||||||
Sbjct 204 GAACCAGTCTCCCATGTTGTTGCTCGTCCTTCCCCAAATGACATTCTCGAGTGGCATTAT 263
Query 373 GTGTTGGAAGGCAGTGAGGGAACACCTTTTGCAGGTGGATTTTACTATGGGAAGATCAAG 432
||| |||||||||||||||||||||||||||||||||||||||||||||| |||||||||
Sbjct 264 GTGCTGGAAGGCAGTGAGGGAACACCTTTTGCAGGTGGATTTTACTATGGAAAGATCAAG 323
Query 433 TTCCCTCCAGAATATCCTTACAAGCCACCTGGAATAACAATGACCACACCAAATGGTCGA 492
|| ||||| || ||||||||||||||||| || || |||||||| |||||||||||||||
Sbjct 324 TTTCCTCCCGAGTATCCTTACAAGCCACCAGGGATTACAATGACTACACCAAATGGTCGA 383
Query 493 TTTGTGACACAAAAGAAAATCTGTTTGTCTATGAGTGACTTTCATCCAGAAAGCTGGAAT 552
|| ||||||| |||||||| ||||| ||||||||||| |||||||||||||||||||||
Sbjct 384 TTCATGACACAGAAGAAAATTTGTTTATCTATGAGTGATTTTCATCCAGAAAGCTGGAAT 443
Query 553 CCAATGTGGTCAGTGTCAAGCATACTTACAGGACTTCTCTCATTTATGATGGACAACAGC 612
|| |||||||| || |||||||||||||||||||||||||||||||||||||| | |||
Sbjct 444 CCGATGTGGTCTGTATCAAGCATACTTACAGGACTTCTCTCATTTATGATGGATACCAGT 503
Query 613 CCCACAACGGGAAGTGTAAACACTAGTGTAGCCGATAAACAACAGTTGGCGAAGTCATCT 672
|| ||||| |||||||| ||||||| |||| || ||||||| |||||| |||||||||
Sbjct 504 CCGACAACTGGAAGTGTTAACACTACTGTAATTGAGAAACAACGGTTGGCTAAGTCATCT 563
Query 673 CTTGCTTTTAATTGTAAAAATGCAACATTCCGGAAA-CTATTTCCCGAGTATGTAGAGAA 731
|| ||||| |||||||||| || |||| || ||| |||||||| ||||||||||||||
Sbjct 564 CTCGCTTTCAATTGTAAAACCCCAGCATTTCG-AAAGCTATTTCCAGAGTATGTAGAGAA 622
Query 732 GTACAGCCAGCAACAAGTAGCTGAAGAGGAAGC-AGCCACTCAACAGACAACACCTGAGG 790
||||| |||||| ||| ||||||| | |||| | |||| |||| ||||||||| |||
Sbjct 623 GTACAACCAGCAGCAACTAGCTGA-GC--AAGCTA-CCACACAACTGACAACACCAGAG- 677
Query 791 ATTCTCCTCAGGAG-GAAAATGCGAAAGTCGAATCAGAGAAAAGCATTGGTCTTAA-AAA 848
| ||||||| || | | | | |||||||| |||||||||| ||| | || || |||
Sbjct 678 -T-CTCCTCAAAAGAGCGA-TACCAAAGTCGAGTCAGAGAAAACCATCGATCC-AACAAA 733
Query 849 GGACAGTATTCAAGAAG-TGGGATTGAAAGAGAGGAGAAAGAACAAGAAAGAAGCATTAC 907
|| || ||||| |||| ||| ||||| ||||||| ||||||||| ||| | | ||| |
Sbjct 734 GGG-AG-ATTCA-GAAGGTGGC-TTGAAGGAGAGGAAAAAGAACAATAAACAGGGATTGC 789
Query 908 CGG-GTTGGATAGTATTGCTGCTAGTATCGATTGTCGGTGTTGTAATGGCGTTGCCTTTG 966
| | || ||||| || || ||||||| ||| || ||||||| ||||||||||||||| ||
Sbjct 790 CAGCGT-GGATAATACTGTTGCTAGTGTCGGTTTTCGGTGTGGTAATGGCGTTGCCTCTG 848
Query 967 CTTCAACTGTGATTTAGATGCAA 989
||||||||||||||| ||||||
Sbjct 849 CTTCAACTGTGATTTCTATGCAA 871
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 57379222935
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5