BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg496564
Length=3349
Score E
Sequences producing significant alignments: (Bits) Value
AT5G63770.1 | Symbols: ATDGK2, DGK2 | diacylglycerol kinase 2 |... 4176 0.0
> AT5G63770.1 | Symbols: ATDGK2, DGK2 | diacylglycerol kinase 2
| chr5:25518641-25523621 FORWARD LENGTH=3580
Length=3580
Score = 4176 bits (2261), Expect = 0.0
Identities = 2538/2669 (95%), Gaps = 30/2669 (1%)
Strand=Plus/Plus
Query 1 CTCTCTCGCTGGCTTCGAGGAG-AAAAAGCAAAATTGGAGACATAATTTTACTGGTTCTC 59
||| |||||||||||||||||| |||||| ||||||||||| ||||||| ||||||||||
Sbjct 20 CTCGCTCGCTGGCTTCGAGGAGAAAAAAGAAAAATTGGAGAAATAATTTAACTGGTTCTC 79
Query 60 GGAGATTCTAGGGGttttttttttttttA-TCATCACCTTGCTGATCTCCGTCGTGAATT 118
||||| |||| ||| || |||||| || | |||||||||||||| |||||||||
Sbjct 80 GGAGAATCTA-GGG------TTCTTTTTAGCCA-CGCCTTGCTGATCTCCTTCGTGAATT 131
Query 119 CG-TCTCCGATTCTCACCTGGTATTTTGTGGCCTAAAGCTCATAAGTTTATGCTGGTGCA 177
|| | ||||||||||||||||| ||||||||||||||||||||||||||||||| |||||
Sbjct 132 CGAT-TCCGATTCTCACCTGGTCTTTTGTGGCCTAAAGCTCATAAGTTTATGCTAGTGCA 190
Query 178 ACAAGTTAAACAGGAATGACGTATTAGATTTGCAATGATGGAAGTGGGGTTCTCTCTTAT 237
| |||||| | ||||||||| |||||||||| ||||||||||||||||||||||||||||
Sbjct 191 AGAAGTTATATAGGAATGACATATTAGATTTACAATGATGGAAGTGGGGTTCTCTCTTAT 250
Query 238 TCAGTGGTTGATCAGTTCTGGTGCTGACAGTCCTTTTATATTTGGATGGCTTGTTACTGG 297
||||||||||||||||||||||||||||||||| |||||||||||||||||||| |||||
Sbjct 251 TCAGTGGTTGATCAGTTCTGGTGCTGACAGTCCGTTTATATTTGGATGGCTTGTAACTGG 310
Query 298 ATCTGTTGGGCTTCTCGCTGTTATCTACACATTTCTTAGATGGCAGaaaaaaaCTTCTTT 357
||||||||| ||||| |||||||||||||||||||||| |||||||||||||||||||||
Sbjct 311 ATCTGTTGGCCTTCTGGCTGTTATCTACACATTTCTTAAATGGCAGAAAAAAACTTCTTT 370
Query 358 AAATTGGGTTAAAGCTGCTGCTAGAGAGAAGAAGAAGGTTTGGAAAAGACTGAGAGTTCC 417
||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 371 AAATTGGGTTAAAGCTGCTGCTAGAGAGAAGAAGAAGGTATGGAAAAGACTGAGAGTTCC 430
Query 418 ACTTTCTCATCATCAATGGACCGATGATTATGGTTATGGCCCACAGCCATCCACTTGCTG 477
||||||||||||||||||||| ||||||||||||||||| | ||||||||||||||||||
Sbjct 431 ACTTTCTCATCATCAATGGACTGATGATTATGGTTATGGGCAACAGCCATCCACTTGCTG 490
Query 478 TGTTTGTCTCTACTCCCTTGTTCCAGGTCAAAATGTTTCTACTAAAGCAGCTCTGAGTAT 537
||||||||||||||||||||||||||||||||||||||||| ||||||| ||||||||||
Sbjct 491 TGTTTGTCTCTACTCCCTTGTTCCAGGTCAAAATGTTTCTAATAAAGCATCTCTGAGTAT 550
Query 538 TCCCGTCCACCGGTGTGCTGTATGCGGTGTAGCAGCTCACTTTTATTGCTCAGGCTCTGC 597
|||||||||||| ||||||||||||||||||||||||||||||||||||||| |||||||
Sbjct 551 TCCCGTCCACCGATGTGCTGTATGCGGTGTAGCAGCTCACTTTTATTGCTCAAGCTCTGC 610
Query 598 TGCAAAAGACTGCAAATGTGTTGCGCAGGCTGGATCTGACCATGTCCGACATCACTGGTC 657
||||||||||||||||||||||| |||||||| ||||||||||||||||||||||||||
Sbjct 611 AGCAAAAGACTGCAAATGTGTTGCACAGGCTGGGTCTGACCATGTCCGACATCACTGGTC 670
Query 658 TGAGCGATGGGTTAACATGGATGATAGCGCTGACATGACTGCTTTTTGCTTCTACTGTGA 717
|||||||||||||||||||||||||| |||||||||||||| |||||||||||||||||
Sbjct 671 TGAGCGATGGGTTAACATGGATGATAATGCTGACATGACTGCCTTTTGCTTCTACTGTGA 730
Query 718 TGAACCGTGTGGTATTCCTTTCATTGAAGCTTCTCCAATGTGGCACTGTCTTTGGTGCCA 777
|||||| ||||||||||||||||||||||||||||| |||||||||||||||||||||||
Sbjct 731 TGAACCTTGTGGTATTCCTTTCATTGAAGCTTCTCCTATGTGGCACTGTCTTTGGTGCCA 790
Query 778 GCGCCTCATACATGTAAAGTGCCACATGATTATGTCCAAGGAATCTGGTGACGCTTGTGA 837
||||||||||||||| ||||| ||||||||||||||||||||||||||||| ||||||||
Sbjct 791 GCGCCTCATACATGTTAAGTGTCACATGATTATGTCCAAGGAATCTGGTGATGCTTGTGA 850
Query 838 TCTTGGCTCTCTCAGAAGAGTAATCCTCTCTCCAGTACATGTAAAACTCAATGAAGCAAA 897
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 851 TCTTGGCTCTCTCAGAAGAGTAATCCTCTCTCCAGTACATGTAAAACTCAATGAAGCAAA 910
Query 898 TGGTGTGGATGGAGTACTGAGTACTATTAAAAACGAGTTAGCATCTATACGAGGGCATGT 957
|||||| ||||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 911 TGGTGTCGATGGAGTACTGACTACTATTAAAAACGAGTTAGCATCTATACGAGGGCATGT 970
Query 958 GAGAAGAAAGCGTCATAGGGGAAAAAATGGGAATGGCCAGTCACTAAATGGTAAGCTGTT 1017
|||||||||||| || ||||||||||||||||||||||| || ||||||||||||| |
Sbjct 971 GAGAAGAAAGCGCCACAGGGGAAAAAATGGGAATGGCCAATCTTTAAATGGTAAGCTACT 1030
Query 1018 GGAGGATTCAGCCAGCAATCCAGTAAAAACCGTAGTAAATGGGCTTGTAGTGAA-AAAGA 1076
||||||||||| |||| ||||||||||||||||||| ||||||||||||||||| |||
Sbjct 1031 GGAGGATTCAGTCAGCGATCCAGTAAAAACCGTAGTTAATGGGCTTGTAGTGAAGAAA-C 1089
Query 1077 TACGCAGAGATAGGAGCATAGATTGCTTACAGAAAGTCTCGGACATGCCTAATG-CAAAC 1135
||||||||||||||||||||||||||||| || ||||||||||||||||||||| ||||
Sbjct 1090 TACGCAGAGATAGGAGCATAGATTGCTTAAAGCAAGTCTCGGACATGCCTAATGCCAAA- 1148
Query 1136 GGATTGCAAAATGGTATAAGCGGCCACAAACGAAATAAAAGTGCCGCTTTTAATTTTATG 1195
|| ||||||||||||||| |||| |||||||||||||||||||||||||| |||||||||
Sbjct 1149 GGGTTGCAAAATGGTATAGGCGGGCACAAACGAAATAAAAGTGCCGCTTTAAATTTTATG 1208
Query 1196 AAGAAGTTTTCCTTGGTTGATTTGCCCCCAGATGCAAGACCGCTTTTGGTCTTTATTAAT 1255
||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1209 AAGAAGTTTTCCTTGGTTGATTTGCCCCCAGATGCAAGACCGCTTTTAGTCTTTATTAAC 1268
Query 1256 GCCAAAAGCGGAGGGCAACTCGGTCCTTTTCTTCACAGGAGACTTAACATGCTGTTGAAT 1315
|||||||||||||| |||||||||||||||||||||||||||||||| ||||| ||||||
Sbjct 1269 GCCAAAAGCGGAGGTCAACTCGGTCCTTTTCTTCACAGGAGACTTAATATGCTATTGAAT 1328
Query 1316 CCAGTACAGGTCTTTGAACTTGGCTCTTGTCAAGGGCCAGACGCTGGTTTAGACCTATGT 1375
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1329 CCAGTACAGGTCTTTGAACTTGGCTCTTGTCAAGGGCCAGACGCTGGTTTAGACCTATGT 1388
Query 1376 AGCAAAGTGAAGTATTTTAGAGTTTTGGTATGTGGGGGAGATGGAACAGTTGCATGGGTG 1435
||||||||||||||||||||||||||||| ||||| ||||||||||||||||||||||||
Sbjct 1389 AGCAAAGTGAAGTATTTTAGAGTTTTGGTGTGTGGAGGAGATGGAACAGTTGCATGGGTG 1448
Query 1436 CTTGATGCCATTGAGAAGCGCAATTTTGAATCCCCTCCACCTGTCGCTATTCTTCCTTTA 1495
|||||||| ||||||||||||||||| |||||||||||||||||||||||||||||||||
Sbjct 1449 CTTGATGCTATTGAGAAGCGCAATTTCGAATCCCCTCCACCTGTCGCTATTCTTCCTTTA 1508
Query 1496 GGCACAGGAAATGATTTATCTAGAGTTTTGCAGTGGGGAAGAGGTATTTCAGTAGTTGAT 1555
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1509 GGCACAGGAAATGATTTATCTAGAGTTTTGCAGTGGGGAAGAGGTATTTCAGTAGTTGAT 1568
Query 1556 GGACAAGGAAGTCTAAGAACGTTTTTGCAAGACATTGATCATGCTGCAGTTACAATGTTG 1615
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1569 GGACAAGGAAGTCTAAGAACGTTTTTGCAAGACATTGATCATGCTGCAGTTACAATGTTG 1628
Query 1616 GATCGATGGAGTGTTAAAATTGTAGAAGAGAGTACAGAAAAATTTCCAGCAAGAGAAGGC 1675
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1629 GATCGATGGAGTGTTAAAATTGTAGAAGAGAGTACAGAAAAATTTCCAGCAAGAGAAGGC 1688
Query 1676 CACAAGTTTATGATGAATTACTTAGGTATTGGCTGTGACGCAAAAGTTGCATATGAATTT 1735
|| |||||||||||||||||||||||||||||||| || |||||||||||||||||||||
Sbjct 1689 CATAAGTTTATGATGAATTACTTAGGTATTGGCTGCGATGCAAAAGTTGCATATGAATTT 1748
Query 1736 CATATGATGCGGCAAGA-AAATCCTCAAAAGTTTTGTAGTCAGTTTGTAAATAAATTACG 1794
||||||||||||||||| ||| ||| | ||||||||||||||||||||||||||||||||
Sbjct 1749 CATATGATGCGGCAAGAGAAA-CCTGAGAAGTTTTGTAGTCAGTTTGTAAATAAATTACG 1807
Query 1795 ATATGCTAAAGAAGGTGCGAGGGATATAATGGATCGAGCATGTGCCGATTTACCATGGCA 1854
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1808 ATATGCTAAAGAAGGTGCGAGGGATATAATGGATCGAGCATGTGCCGATTTACCATGGCA 1867
Query 1855 AGTTTGGCTTGAAGTTGATGGAAAAGACATTGAAATTCCCAAGGATTCAGAGGGTCTAAT 1914
||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 1868 AGTTTGGCTTGAAGTTGATGGAAAAGACATTGAAATTCCTAAGGATTCAGAGGGTCTAAT 1927
Query 1915 AGTACTTAACATTGGAAGCTATATGGGTGGAGTAGATCTTTGGCAAAATGATTATGAACA 1974
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1928 AGTACTTAACATTGGAAGCTATATGGGTGGAGTAGATCTTTGGCAAAATGATTATGAACA 1987
Query 1975 TGATGATAATTTTAGCATTCAGTGTATGCACGACAAGACACTAGAAGTGGTATGTGTCCG 2034
|||||||||||||||||||||||||||||||||||||| ||||| ||||||||||||||
Sbjct 1988 CGATGATAATTTTAGCATTCAGTGTATGCACGACAAGACTCTAGAGGTGGTATGTGTCCG 2047
Query 2035 TGGAGCGTGGCACCTTGGCAAACTACAGGTCGGTCTTTCTCAGGCTAGGAGGCTAGCACA 2094
|||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
Sbjct 2048 TGGAGCGTGGCACCTTGGCAAACTACAGGTCGGTCTTTCTCAGGCTAGGAGGTTAGCACA 2107
Query 2095 GGGGAAGGTCATAAGGATACATGTTTCTAGTCCTTTCCCTGTTCAAATAGATGGAGAACC 2154
||| ||||| ||||| |||||||||||||||||||| |||||||||||||||||||||||
Sbjct 2108 GGGAAAGGTAATAAGAATACATGTTTCTAGTCCTTTTCCTGTTCAAATAGATGGAGAACC 2167
Query 2155 GTTTATTCAGCAACCTGGCTGCTTGGAGATAACGCACCACGGGCAGGTGTTTATGCTAAG 2214
|||||| |||||||||||||||||||||||||| ||||||||||||||||||||||||||
Sbjct 2168 GTTTATCCAGCAACCTGGCTGCTTGGAGATAACTCACCACGGGCAGGTGTTTATGCTAAG 2227
Query 2215 AAGAGCATCGGATGAACCACGAGGGCATGCCGCAGCCATAATGAATGAGGTGTTACTCGA 2274
|||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 2228 AAGAGCATCGGATGAACCACGAGGGCATGCCGCAGCCATAATGAATGAAGTGTTACTCGA 2287
Query 2275 TGCGGAATGCAAAGGAGTCATAAATGCATCTCAAAAGAAAGTACTTCTACAGCAAATGGC 2334
||| ||||||||||||||||||||||||||||||||||||||||| ||||||||||||||
Sbjct 2288 TGCAGAATGCAAAGGAGTCATAAATGCATCTCAAAAGAAAGTACTACTACAGCAAATGGC 2347
Query 2335 TCTCCATCTCTCATAG-aaaaaa-caaaaaCAGATCATCCTCTCACCAAATTTTGCTAAC 2392
|||||||||||| ||| |||||| ||||||||||||||||||||||||||||||| ||||
Sbjct 2348 TCTCCATCTCTCGTAGTAAAAAAACAAAAACAGATCATCCTCTCACCAAATTTTGGTAAC 2407
Query 2393 AAGAAGAGTTAACTTACAATCTTGTCTTACCCACTAGAAAGCCAG-CCCGGGTACGAGAt 2451
||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||
Sbjct 2408 AAGAAGAGTTAACTTACAATCTTGTCTTACCCACTAGAAAGCCAGTCCCGGGTACGAGAT 2467
Query 2452 tttttttgtctatttacccttattttttgttttt-tttt-ttttCCCTTTGGTGTTTGTG 2509
||||| ||||||||| ||||| ||||||||| |||| |||| |||||||||||||
Sbjct 2468 TTTTT--GTCTATTTAACCTTAATTTTTGTTTCAATTTTGTTTTGT-TTTGGTGTTTGTG 2524
Query 2510 AAGAGTGTCATATGTATAGATAGTGTGCCGC-TACGAAAGCCGCTGAGATTGGCGTCAAA 2568
||||||| ||||||||||||||||||||| | |||||||||| |||||||||||||||||
Sbjct 2525 AAGAGTGCCATATGTATAGATAGTGTGCC-CATACGAAAGCCACTGAGATTGGCGTCAAA 2583
Query 2569 AAGTGTCAACTAGAGAGAGTTTGATTATATACATCATACAGTGATCAGTTTTAGGGTAAT 2628
||||||||| |||||| ||||||||||||||||||||||||||||| |||||||||||||
Sbjct 2584 AAGTGTCAAGTAGAGAAAGTTTGATTATATACATCATACAGTGATCGGTTTTAGGGTAAT 2643
Query 2629 ACAAAGGCCATTTATTACCCATGAGTTTT 2657
|||||||| | |||||||||||||||||
Sbjct 2644 ACAAAGGC--TCTATTACCCATGAGTTTT 2670
Score = 296 bits (160), Expect = 9e-79
Identities = 184/196 (94%), Gaps = 0/196 (0%)
Strand=Plus/Plus
Query 2770 AAAATAACATGATGTTATATTTGTGGAATCATTTTATACCAAACACGGAATATTTTGTTC 2829
|||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2848 AAAATACCATGATGTTATATTTGTGGAATCATTTTATACCAAACACGGAATATTTTGTTC 2907
Query 2830 TAGGGAATCAATGATATTTTGGCAAATAACTAGTTGCTAAAATTTAACTATCATTTAATT 2889
|||||||||||||||||||||| ||||| || || ||||| |||| |||||||||||||
Sbjct 2908 AAGGGAATCAATGATATTTTGGCTAATAAGTACTTACTAAAGTTTATCTATCATTTAATT 2967
Query 2890 ACCTTACTAATTTTGTTATTTCCTTAAATATTGGGATTATCCAGAGCAATTAGACTTTTG 2949
||||||||| ||||||||||||||||||||||||| ||||||||||||||||| ||||||
Sbjct 2968 ACCTTACTAGTTTTGTTATTTCCTTAAATATTGGGCTTATCCAGAGCAATTAGTCTTTTG 3027
Query 2950 TATCATTAACATAATT 2965
||||||||||| ||||
Sbjct 3028 TATCATTAACAGAATT 3043
Score = 237 bits (128), Expect = 5e-61
Identities = 161/177 (91%), Gaps = 2/177 (1%)
Strand=Plus/Plus
Query 3173 TATTATTTGATTTACACAATAAAAATGAATAATGCTTGGATCTAGTTTAATAAAAAATAA 3232
||||||||||||| | ||||||||||| || ||||||||||||||||||| |||| |||
Sbjct 3392 TATTATTTGATTTGCTCAATAAAAATGTATGATGCTTGGATCTAGTTTAA-GAAAATTAA 3450
Query 3233 ATCTAATTTCTAATTCTGCTAGATTATATTGGACTCTTTAGTTAATAAAATGATAATTAT 3292
||||||||||||||||||||||||||||||||||| |||||| || ||| ||||||||||
Sbjct 3451 ATCTAATTTCTAATTCTGCTAGATTATATTGGACTATTTAGTCAACAAA-TGATAATTAT 3509
Query 3293 AATATGAAAGGATATAAATTTGAATACGACAATTAAATTACTTATTTCAACAATTTG 3349
| ||| ||| ||||||| ||||||||||||||||||||||||||||||||| |||||
Sbjct 3510 ATTATCAAAAGATATAATTTTGAATACGACAATTAAATTACTTATTTCAACTATTTG 3566
Score = 152 bits (82), Expect = 2e-35
Identities = 137/160 (86%), Gaps = 17/160 (11%)
Strand=Plus/Plus
Query 3034 AAGTGGATAAATAAAACAGCTCCTCTCCATCCATTATGCGACATCCAGAA-AAAATATCT 3092
||||||||||||||||||||| || ||||||||| ||||||| |||| || |||||||||
Sbjct 3211 AAGTGGATAAATAAAACAGCTTCTTTCCATCCATCATGCGACCTCCA-AAGAAAATATCT 3269
Query 3093 ACAGAAAGAAACAGAATAATTTCAAATTTTCAGAGCAAAA-AAT-T-A-GA--------G 3140
|||||||||||||||||||||||||| || |||||||||| ||| | | || |
Sbjct 3270 ACAGAAAGAAACAGAATAATTTCAAAATT-CAGAGCAAAATAATATCAAGAAAAAATTAG 3328
Query 3141 --CCAAAAAGAGACATATCATTTGATTTTAGGGATATTAT 3178
|||||||||||||| |||||||||||||||||||||||
Sbjct 3329 AACCAAAAAGAGACATGTCATTTGATTTTAGGGATATTAT 3368
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 166654584646
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5