BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg889357
Length=1557
Score E
Sequences producing significant alignments: (Bits) Value
AT4G27850.1 | Symbols: | Glycine-rich protein family | chr4:13... 448 1e-124
AT4G27852.1 | Symbols: | Potential natural antisense gene, loc... 255 7e-67
> AT4G27850.1 | Symbols: | Glycine-rich protein family | chr4:13870382-13873509
FORWARD LENGTH=1734
Length=1734
Score = 448 bits (242), Expect = 1e-124
Identities = 443/537 (82%), Gaps = 26/537 (5%)
Strand=Plus/Plus
Query 157 GGAACCGAGAGAGAAG-GCACCAACAATAATGAATCTCAAGTTATACATGAG-GTGGCGA 214
|||||||||||||||| | | ||||||||||||||||||| | | | ||| | |||
Sbjct 76 GGAACCGAGAGAGAAGAG-AATAACAATAATGAATCTCAAG-AAGAGACGAGAACGTCGA 133
Query 215 GAAGTCCTAATCATGCTTTGGATGAGATAAAGAAGCAACAGACTCATAATCTCTACTGTC 274
||||||| ||| | |||||||||||||||| || ||||||||| ||||| ||||||||||
Sbjct 134 GAAGTCCCAATGAGGCTTTGGATGAGATAAGGAGGCAACAGACGCATAACCTCTACTGTC 193
Query 275 CTAAATGTAAAAGTAACATCACC-ACAGATGCTGTACTCTTTGTAAAGAATCAAGAAACC 333
|||||||||||| |||||||||| | || |||| |||| |||||||||||||||| |||
Sbjct 194 CTAAATGTAAAACTAACATCACCAAAAG-CGCTGAACTCGTTGTAAAGAATCAAGAGACC 252
Query 334 GATTCGTACAAAACAGAGCCTTACGTACTTTGGGTTCCCCTCGTTATCCCGGCCATCAAG 393
||||||||||||||| || ||||||||||||||||||||||||||||| || || || ||
Sbjct 253 GATTCGTACAAAACAAAGGCTTACGTACTTTGGGTTCCCCTCGTTATCTCGCCCTTCGAG 312
Query 394 TTCCCTACCTTTGATCTCTCGTTGTCTTTTCTCGCAGGACCATGGAAGAAACTAAAGACA 453
||||||||| || |||||| |||||||||||| |||||| |||||||||||||||||||
Sbjct 313 TTCCCTACCCTTCATCTCTTATTGTCTTTTCTCACAGGACTATGGAAGAAACTAAAGACA 372
Query 454 CTTGTCGCAAACTGTTTGCCAAG--TC--TC--AA-TT-CTCCACGTTTGCGGTTCCTAT 505
|||||| ||||||||||| || | || || || || || | | | |||||| ||||
Sbjct 373 CTTGTCCCAAACTGTTTGTCACGGCTCAGTCCCAACTTTCTGC-C-TCTGCGGTACCTAC 430
Query 506 TGGTTGCGGTTCTACTATTGCTATCAGTCATTGTTCTGTGGTCCTCTCATCCCCCT-TCG 564
|||||||||||||||||||||||||||||||||| |||||||||||| |||| ||| | |
Sbjct 431 TGGTTGCGGTTCTACTATTGCTATCAGTCATTGTCCTGTGGTCCTCTGATCCTCCTCT-G 489
Query 565 CCACCGCCACTGCCACCGCCTCCTTCTCC-TTCGCCAGTGCCGG-T--GCCGCCACCG-C 619
||||| |||| |||||| |||||| || || |||| ||| | ||| |||| |
Sbjct 490 CCACCACCACCGCCACCTTATCCTTCACCATT-GCCACCGCCTCCTTCGCCTTCACCAAC 548
Query 620 -C-AGGACCACCTTCTCCTTCGCCATCGCCAGAGCCTCCTTCTCCTTCGCCAGTGCC 674
| ||| || |||||||| ||||||||||| ||| |||||||| |||| ||||
Sbjct 549 ACCAGGGCCGGATTCTCCTTTGCCATCGCCAGGGCCGGATTCTCCTTTGCCACTGCC 605
Score = 255 bits (138), Expect = 7e-67
Identities = 204/235 (87%), Gaps = 8/235 (3%)
Strand=Plus/Plus
Query 1321 CGACAAGGGACACTACAAAGCTGCGGTTGTTCTCGCTGTATCTTTGGTCTGCGTGATCT- 1379
||||||||||||||||||||||||||||||||| |||| |||| |||||||||| ||||
Sbjct 1500 CGACAAGGGACACTACAAAGCTGCGGTTGTTCTTGCTGCATCTCTGGTCTGCGTCATCTC 1559
Query 1380 TACTCTCCTTTGCCAAAAAGCTTACGCCTTCCGAATGGGAAGAGTCAAGACCGTGGCTGA 1439
| ||||||||||||||| || ||||| ||||| ||| || | ||| |||| |||||||
Sbjct 1560 T-CTCTCCTTTGCCAAA--GCCTACGCTTTCCGCATGCAAAAACTCAGGACCATGGCTGA 1616
Query 1440 ATACACCGGAATGGCGATTGGAGCGTCTGCTCTTT-CTTTCATCGCCAGCCAAATCG-CT 1497
|||||||||||||||| |||| || ||||| |||| | ||||| ||||||| ||||| |
Sbjct 1617 ATACACCGGAATGGCGTTTGGGGCATCTGC-CTTTTCATTCATTGCCAGCCGAATCGTC- 1674
Query 1498 AGTGATATCTTTGAAAAGTTAGGCTTTCATGAGCTTGCCTCTGAATACCGAAAAG 1552
|||||||||||||||||||| ||||||||||||||||| |||| ||||||||||
Sbjct 1675 AGTGATATCTTTGAAAAGTTTGGCTTTCATGAGCTTGCAGCTGAGTACCGAAAAG 1729
Score = 228 bits (123), Expect = 1e-58
Identities = 137/144 (95%), Gaps = 0/144 (0%)
Strand=Plus/Plus
Query 1055 TGTGGAAATATATCGTGGTTCAAGTTCAAAACCTCGTACCAAGAGAGCTAGACATCTTGA 1114
||| ||||||||||||||||| ||||||||||| | ||||||||||||||||||||||||
Sbjct 1350 TGTTGAAATATATCGTGGTTCTAGTTCAAAACCACTTACCAAGAGAGCTAGACATCTTGA 1409
Query 1115 AGAGTATTGTTTACGGAGGTCTTATCGAGTCCATCACAAGCCTCGGTGTCGTATCCTCTG 1174
|||||||||| ||||||||||||||||||||||||||||||||||||||||||||||| |
Sbjct 1410 AGAGTATTGTGTACGGAGGTCTTATCGAGTCCATCACAAGCCTCGGTGTCGTATCCTCCG 1469
Query 1175 CTATCGCTTCCGGAGCTTCAACTC 1198
|| |||||||||||||||||||||
Sbjct 1470 CTGTCGCTTCCGGAGCTTCAACTC 1493
Score = 180 bits (97), Expect = 4e-44
Identities = 115/124 (93%), Gaps = 0/124 (0%)
Strand=Plus/Plus
Query 740 TCATACATTTTCTGAGTATCAAGTACCTTTCCGTATTCTCGTTATTGTTCCTTGCCATTC 799
|||||||||| | |||||||||||||||||| ||||||||| ||||||||||||||||||
Sbjct 890 TCATACATTTCCCGAGTATCAAGTACCTTTCTGTATTCTCGCTATTGTTCCTTGCCATTC 949
Query 800 TGACTCTCCCCTGGCCTTCCATTTCCCCTATCATCAATAACCTTATAGCAGCAATTAAAA 859
|||||||||| ||| ||||||||||||| ||| ||||||||||||| |||||||||||||
Sbjct 950 TGACTCTCCCTTGGGCTTCCATTTCCCCCATCTTCAATAACCTTATTGCAGCAATTAAAA 1009
Query 860 CATG 863
||||
Sbjct 1010 CATG 1013
> AT4G27852.1 | Symbols: | Potential natural antisense gene, locus
overlaps with AT4G27850 and AT4G27860 | chr4:13872079-13873907
REVERSE LENGTH=1589
Length=1589
Score = 255 bits (138), Expect = 7e-67
Identities = 204/235 (87%), Gaps = 8/235 (3%)
Strand=Plus/Minus
Query 1321 CGACAAGGGACACTACAAAGCTGCGGTTGTTCTCGCTGTATCTTTGGTCTGCGTGATCT- 1379
||||||||||||||||||||||||||||||||| |||| |||| |||||||||| ||||
Sbjct 393 CGACAAGGGACACTACAAAGCTGCGGTTGTTCTTGCTGCATCTCTGGTCTGCGTCATCTC 334
Query 1380 TACTCTCCTTTGCCAAAAAGCTTACGCCTTCCGAATGGGAAGAGTCAAGACCGTGGCTGA 1439
| ||||||||||||||| || ||||| ||||| ||| || | ||| |||| |||||||
Sbjct 333 T-CTCTCCTTTGCCAAA--GCCTACGCTTTCCGCATGCAAAAACTCAGGACCATGGCTGA 277
Query 1440 ATACACCGGAATGGCGATTGGAGCGTCTGCTCTTT-CTTTCATCGCCAGCCAAATCG-CT 1497
|||||||||||||||| |||| || ||||| |||| | ||||| ||||||| ||||| |
Sbjct 276 ATACACCGGAATGGCGTTTGGGGCATCTGC-CTTTTCATTCATTGCCAGCCGAATCGTC- 219
Query 1498 AGTGATATCTTTGAAAAGTTAGGCTTTCATGAGCTTGCCTCTGAATACCGAAAAG 1552
|||||||||||||||||||| ||||||||||||||||| |||| ||||||||||
Sbjct 218 AGTGATATCTTTGAAAAGTTTGGCTTTCATGAGCTTGCAGCTGAGTACCGAAAAG 164
Score = 228 bits (123), Expect = 1e-58
Identities = 137/144 (95%), Gaps = 0/144 (0%)
Strand=Plus/Minus
Query 1055 TGTGGAAATATATCGTGGTTCAAGTTCAAAACCTCGTACCAAGAGAGCTAGACATCTTGA 1114
||| ||||||||||||||||| ||||||||||| | ||||||||||||||||||||||||
Sbjct 1074 TGTTGAAATATATCGTGGTTCTAGTTCAAAACCACTTACCAAGAGAGCTAGACATCTTGA 1015
Query 1115 AGAGTATTGTTTACGGAGGTCTTATCGAGTCCATCACAAGCCTCGGTGTCGTATCCTCTG 1174
|||||||||| ||||||||||||||||||||||||||||||||||||||||||||||| |
Sbjct 1014 AGAGTATTGTGTACGGAGGTCTTATCGAGTCCATCACAAGCCTCGGTGTCGTATCCTCCG 955
Query 1175 CTATCGCTTCCGGAGCTTCAACTC 1198
|| |||||||||||||||||||||
Sbjct 954 CTGTCGCTTCCGGAGCTTCAACTC 931
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 76857034395
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5