BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg899704
Length=660
Score E
Sequences producing significant alignments: (Bits) Value
AT2G23830.1 | Symbols: | PapD-like superfamily protein | chr2:... 542 2e-153
AT3G60600.1 | Symbols: VAP27-1, VAP, (AT)VAP, VAP27 | vesicle a... 372 3e-102
AT2G45140.1 | Symbols: PVA12 | plant VAP homolog 12 | chr2:1861... 230 2e-59
AT2G23860.1 | Symbols: | pseudogene, similar to VAP27, blastp... 147 2e-34
> AT2G23830.1 | Symbols: | PapD-like superfamily protein | chr2:10143646-10144511
FORWARD LENGTH=450
Length=450
Score = 542 bits (293), Expect = 2e-153
Identities = 353/382 (92%), Gaps = 4/382 (1%)
Strand=Plus/Plus
Query 278 ATG-GTAACAACGAGCTTCTCAAAATCGAGCCTATGTACCTTCAATTCCCTTTTGAATTG 336
||| ||||||||||||||||| |||||||||||||||| |||||||||||||||||||||
Sbjct 1 ATGAGTAACAACGAGCTTCTCGAAATCGAGCCTATGTATCTTCAATTCCCTTTTGAATTG 60
Query 337 AAGAAGCAGATGTCTTGCTCTCTTAACTTGACGAACAAGACAGACAATAATGGGGCCTTT 396
|||||||||||||||||||||||| ||||||||||||||||||||||||| | |||||||
Sbjct 61 AAGAAGCAGATGTCTTGCTCTCTTTACTTGACGAACAAGACAGACAATAACGTGGCCTTT 120
Query 397 AAGGTTAAGACGAAAAATCCGAAAAATTATTGCGTTAGGTCTAATTATGGACTAATTCTT 456
||||||||||||| ||||| ||| || |||||||||||| ||||||||||||| ||||||
Sbjct 121 AAGGTTAAGACGACAAATCGGAACAACTATTGCGTTAGGCCTAATTATGGACTTATTCTT 180
Query 457 CCAAAGTCAACTTGTAAAGTTCTTGTGACAATGCAAGCCCAAAAGGAAGTTCTTAATTCT 516
|||||||| |||||||||||||||||||| |||||||| ||||||||||||| | | ||
Sbjct 181 CCAAAGTCGACTTGTAAAGTTCTTGTGACCATGCAAGCTCAAAAGGAAGTTCCT--T-CT 237
Query 517 GATATGCAGAGCAATGAAAAGTTTATGATTCAAAGTGTAATAGCTAGTCCTGCAGTCACA 576
|||||||||||| ||||||||||||||||||||||||| | |||||||||| |||||||
Sbjct 238 GATATGCAGAGCTTTGAAAAGTTTATGATTCAAAGTGTACTGGCTAGTCCTGGAGTCACA 297
Query 577 GCCAAAGAAGTTACTCGTGAGACGTTTAGGAAAGAATCAGGGCATCTAGTTGAGGAGACT 636
|||||||||||||||||||||| |||||| ||||||||||||||| ||||||||||||||
Sbjct 298 GCCAAAGAAGTTACTCGTGAGATGTTTAGCAAAGAATCAGGGCATGTAGTTGAGGAGACT 357
Query 637 AAACTGAAAGTTACTTATTTTT 658
||||||| |||||||||| |||
Sbjct 358 AAACTGAGAGTTACTTATGTTT 379
> AT3G60600.1 | Symbols: VAP27-1, VAP, (AT)VAP, VAP27 | vesicle
associated protein | chr3:22400395-22402702 FORWARD LENGTH=1207
Length=1207
Score = 372 bits (201), Expect = 3e-102
Identities = 333/396 (84%), Gaps = 12/396 (3%)
Strand=Plus/Plus
Query 264 GCGATCTTAT-GGAGATG-GTAACAACGAGCTTCTCAAAATCGAGCCTAT-GTACCTTCA 320
||||||| || || |||| |||||| ||||||||||| |||||||| | | | |||||
Sbjct 177 GCGATCTGATCGG-GATGAGTAACAGCGAGCTTCTCACCGTCGAGCCTCTCG-ATCTTCA 234
Query 321 ATTCCCTTTTGAATTGAAGAAGCAGATGTCTTGCTCTCT-TAACTTGACGAACAAGACAG 379
||||||||||||||||||||||||||| ||||||||||| || |||||||||||||| |
Sbjct 235 ATTCCCTTTTGAATTGAAGAAGCAGATCTCTTGCTCTCTCTAT-TTGACGAACAAGACCG 293
Query 380 ACAATAATGGGGCCTTTAAGGTTAAGACGAAAAATCCGAAAAATTATTGCGTTAGGTCTA 439
||||||||| ||||||||||||||||||| ||||||||||| ||||| |||||| |||
Sbjct 294 ACAATAATGTTGCCTTTAAGGTTAAGACGACGAATCCGAAAAAGTATTGTGTTAGGCCTA 353
Query 440 ATTA-TGGACTAATTCTTCCAAAGTCAACTTGTAAAGTTCTTGTGACAATGCAAGCCCAA 498
|| | |||| | |||| || | ||| ||||| ||||||||||||| |||||||| |||
Sbjct 354 AT-ACTGGAGTTGTTCTCCCGAGGTCTACTTGCGAAGTTCTTGTGACCATGCAAGCTCAA 412
Query 499 AAGGAAGTTCTTAATTCTGATATGCAGAGCAATGAAAAGTTTATGATTCAAAGTGTAATA 558
||||||| || | | | ||||||||| |||| || |||||| || ||||| |||| |||
Sbjct 413 AAGGAAGCTCCT--TCC-GATATGCAGTGCAAGGACAAGTTTCTGCTTCAAGGTGTGATA 469
Query 559 GCTAGTCCTGCAGTCACAGCCAAAGAAGTTACTCGTGAGACGTTTAGGAAAGAATCAGGG 618
|||||||||| ||||||||||| |||||||||| ||||| |||||| ||||| | ||
Sbjct 470 GCTAGTCCTGGTGTCACAGCCAAGGAAGTTACTCCTGAGATGTTTAGCAAAGAGGCTGGA 529
Query 619 CATCTAGTTGAGGAGACTAAACTGAAAGTTACTTAT 654
|||| ||||||||| |||||||||| ||||||||||
Sbjct 530 CATCGAGTTGAGGAAACTAAACTGAGAGTTACTTAT 565
> AT2G45140.1 | Symbols: PVA12 | plant VAP homolog 12 | chr2:18610806-18613290
FORWARD LENGTH=1262
Length=1262
Score = 230 bits (124), Expect = 2e-59
Identities = 292/371 (79%), Gaps = 19/371 (5%)
Strand=Plus/Plus
Query 286 AACGAGCTTCTCAAAATCGAGCCTAT-GTACCTTCAATTCCCTTTTGAATTGAAGAAGCA 344
||||||||||||| ||||| ||| | | |||||||||||||||||||||||||||||||
Sbjct 230 AACGAGCTTCTCACCATCGATCCTGTCG-ACCTTCAATTCCCTTTTGAATTGAAGAAGCA 288
Query 345 GATGTCTTGCTCTCT-TAACTTGACG-AACAAGACAGACAATAATGGGGCCTTTAAGGTT 402
||| ||||| ||||| || ||| | |||||||| || ||| ||| ||||| ||||||
Sbjct 289 GATCTCTTGTTCTCTCTAT-TTGG-GTAACAAGACCGATAATTATGTCGCCTTCAAGGTT 346
Query 403 AAGACGAAAAATCCGAAA-AATTATTGCGTTAGGTCTAATTA-TGGACTAATTCTTCCAA 460
||||||| ||||| ||| || || ||||||||| ||||| | ||| | ||| ||| |
Sbjct 347 AAGACGACGAATCC-AAAGAAGTACTGCGTTAGGCCTAAT-ACTGGTGTTGTTCATCCCA 404
Query 461 -AGTCAACTTGTAAAGTTCTT-GTGACAATGCAAGCCCAAAAGGAAGTTCTTAATTCTGA 518
| || ||| | ||||| || ||||| |||||||| |||||||||| || | ||||
Sbjct 405 GA-TCCTCTTCTGAAGTT-TTAGTGACCATGCAAGCTCAAAAGGAAGCTCCTG---CTGA 459
Query 519 TATGCAGAGCAATGAAAAGTT-TATGATTCAAAGTGTAATAGCTAGTCCTGCAGTCACAG 577
| ||||| | || || ||||| | || ||||| ||||| | |||||||| | || |||
Sbjct 460 TCTGCAGTGTAAAGATAAGTTCT-TGCTTCAATGTGTAGTCGCTAGTCCCGGAGCCACCC 518
Query 578 CCAAAGAAGTTACTCGTGAGACGTTTAGGAAAGAATCAGGGCATCTAGTTGAGGAGACTA 637
|||| || ||||| | ||||| |||||| ||||| ||||||||| |||||| |||||||
Sbjct 519 CCAAGGATGTTACACATGAGATGTTTAGCAAAGAGGCAGGGCATCGAGTTGAAGAGACTA 578
Query 638 AACTGAAAGTT 648
|| ||| ||||
Sbjct 579 AATTGAGAGTT 589
> AT2G23860.1 | Symbols: | pseudogene, similar to VAP27, blastp
match of 56% identity and 1.3e-25 P-value to GP|6688926|emb|CAB65313.1||AJ251365
VAP27 {Nicotiana plumbaginifolia} |
chr2:10157005-10157669 FORWARD LENGTH=665
Length=665
Score = 147 bits (79), Expect = 2e-34
Identities = 111/126 (88%), Gaps = 3/126 (2%)
Strand=Plus/Plus
Query 477 TCTTGTGACAATGCAAGCCCAAAAGGAAGTTC--TTAATTCTGATATGCAGAGCAATGAA 534
||| || || |||||||| |||||||||| | | |||||||||||||||||||||
Sbjct 283 TCTAGTAACCATGCAAGCTCAAAAGGAAGCCCGGCCCA-TCTGATATGCAGAGCAATGAA 341
Query 535 AAGTTTATGATTCAAAGTGTAATAGCTAGTCCTGCAGTCACAGCCAAAGAAGTTACTCGT 594
||||||||||||||||| |||| ||||||||||||||||||||||||||||||||||| |
Sbjct 342 AAGTTTATGATTCAAAGAGTAAAAGCTAGTCCTGCAGTCACAGCCAAAGAAGTTACTCTT 401
Query 595 GAGACG 600
||||||
Sbjct 402 GAGACG 407
Score = 82.4 bits (44), Expect = 5e-15
Identities = 54/59 (92%), Gaps = 0/59 (0%)
Strand=Plus/Plus
Query 600 GTTTAGGAAAGAATCAGGGCATCTAGTTGAGGAGACTAAACTGAAAGTTACTTATTTTT 658
||||| |||||||| |||||||||||||||||||||||||||| |||||||||| |||
Sbjct 512 GTTTAACAAAGAATCGGGGCATCTAGTTGAGGAGACTAAACTGAGAGTTACTTATGTTT 570
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 31898683345
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5