BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg899705
Length=417
Score E
Sequences producing significant alignments: (Bits) Value
AT2G23830.1 | Symbols: | PapD-like superfamily protein | chr2:... 580 3e-165
AT3G60600.1 | Symbols: VAP27-1, VAP, (AT)VAP, VAP27 | vesicle a... 381 3e-105
AT2G45140.1 | Symbols: PVA12 | plant VAP homolog 12 | chr2:1861... 237 6e-62
AT2G23860.1 | Symbols: | pseudogene, similar to VAP27, blastp... 172 2e-42
> AT2G23830.1 | Symbols: | PapD-like superfamily protein | chr2:10143646-10144511
FORWARD LENGTH=450
Length=450
Score = 580 bits (314), Expect = 3e-165
Identities = 384/418 (92%), Gaps = 4/418 (1%)
Strand=Plus/Plus
Query 1 ATGTACCTTCAATTCCCTTTTGAATTGAAGAAGCAGATGTCTTGCTCTCTTAACTTGACG 60
||||| ||||||||||||||||||||||||||||||||||||||||||||| ||||||||
Sbjct 34 ATGTATCTTCAATTCCCTTTTGAATTGAAGAAGCAGATGTCTTGCTCTCTTTACTTGACG 93
Query 61 AACAAGACAGACAATAATGGGGCCTTTAAGGTTAAGACGAAAAATCCGAAAAATTATTGC 120
||||||||||||||||| | |||||||||||||||||||| ||||| ||| || ||||||
Sbjct 94 AACAAGACAGACAATAACGTGGCCTTTAAGGTTAAGACGACAAATCGGAACAACTATTGC 153
Query 121 GTTAGGTCTAATTATGGACTAATTCTTCCAAAGTCAACTTGTAAAGTTCTTGTGACAATG 180
|||||| ||||||||||||| |||||||||||||| |||||||||||||||||||| |||
Sbjct 154 GTTAGGCCTAATTATGGACTTATTCTTCCAAAGTCGACTTGTAAAGTTCTTGTGACCATG 213
Query 181 CAAGCCCAAAAGGAAGTTCTTAATTCTGATATGCAGAGCAATGAAAAGTTTATGATTCAA 240
||||| ||||||||||||| | | |||||||||||||| |||||||||||||||||||
Sbjct 214 CAAGCTCAAAAGGAAGTTCCT--T-CTGATATGCAGAGCTTTGAAAAGTTTATGATTCAA 270
Query 241 AGTGTAATAGCTAGTCCTGGACTCACAGCCAAAGAAGTTACTCGTGAGACGTTAAGGAAA 300
|||||| | |||||||||||| ||||||||||||||||||||||||||| ||| || |||
Sbjct 271 AGTGTACTGGCTAGTCCTGGAGTCACAGCCAAAGAAGTTACTCGTGAGATGTTTAGCAAA 330
Query 301 GAATCGGGGCATCTAGTTGAGGAGACTAAACTGAAAGTTTCTTATGTTTGCTCCACCACG 360
||||| |||||| ||||||||||||||||||||| |||| ||||||||||||||||||||
Sbjct 331 GAATCAGGGCATGTAGTTGAGGAGACTAAACTGAGAGTTACTTATGTTTGCTCCACCACG 390
Query 361 AC-AACATCACCAATTCACCAAGGACCAGAAGAGGGTTTATATTTTAGTGTTTTTGTT 417
|| |||||||| | |||||||||||| |||||||||||||||||| ||||||||||||
Sbjct 391 ACCAACATCACTAGTTCACCAAGGACTAGAAGAGGGTTTATATTTCAGTGTTTTTGTT 448
> AT3G60600.1 | Symbols: VAP27-1, VAP, (AT)VAP, VAP27 | vesicle
associated protein | chr3:22400395-22402702 FORWARD LENGTH=1207
Length=1207
Score = 381 bits (206), Expect = 3e-105
Identities = 333/394 (85%), Gaps = 9/394 (2%)
Strand=Plus/Plus
Query 7 CTTCAATTCCCTTTTGAATTGAAGAAGCAGATGTCTTGCTCTCT-TAACTTGACGAACAA 65
|||||||||||||||||||||||||||||||| ||||||||||| || |||||||||||
Sbjct 230 CTTCAATTCCCTTTTGAATTGAAGAAGCAGATCTCTTGCTCTCTCTAT-TTGACGAACAA 288
Query 66 GACAGACAATAATGGGGCCTTTAAGGTTAAGACGAAAAATCCGAAAAATTATTGCGTTAG 125
||| |||||||||| ||||||||||||||||||| ||||||||||| ||||| |||||
Sbjct 289 GACCGACAATAATGTTGCCTTTAAGGTTAAGACGACGAATCCGAAAAAGTATTGTGTTAG 348
Query 126 GTCTAATTA-TGGACTAATTCTTCCAAAGTCAACTTGTAAAGTTCTTGTGACAATGCAAG 184
| ||||| | |||| | |||| || | ||| ||||| ||||||||||||| |||||||
Sbjct 349 GCCTAAT-ACTGGAGTTGTTCTCCCGAGGTCTACTTGCGAAGTTCTTGTGACCATGCAAG 407
Query 185 CCCAAAAGGAAGTTCTTAATTCTGATATGCAGAGCAATGAAAAGTTTATGATTCAAAGTG 244
| |||||||||| || | | | ||||||||| |||| || |||||| || ||||| |||
Sbjct 408 CTCAAAAGGAAGCTCCT--TCC-GATATGCAGTGCAAGGACAAGTTTCTGCTTCAAGGTG 464
Query 245 TAATAGCTAGTCCTGGACTCACAGCCAAAGAAGTTACTCGTGAGACGTTAAGGAAAGAAT 304
| |||||||||||||| |||||||||| |||||||||| ||||| ||| || |||||
Sbjct 465 TGATAGCTAGTCCTGGTGTCACAGCCAAGGAAGTTACTCCTGAGATGTTTAGCAAAGAGG 524
Query 305 CGGGGCATCTAGTTGAGGAGACTAAACTGAAAGTTTCTTATGTTTGCTCCACCACGACAA 364
| || |||| ||||||||| |||||||||| |||| |||||||| ||||||||||||| |
Sbjct 525 CTGGACATCGAGTTGAGGAAACTAAACTGAGAGTTACTTATGTT-GCTCCACCACGACCA 583
Query 365 C-ATCACCAATTCACCAAGGACCAGAAGAGGGTT 397
| |||||| ||||| ||||| | ||||||||||
Sbjct 584 CCATCACCGGTTCACGAAGGATCTGAAGAGGGTT 617
> AT2G45140.1 | Symbols: PVA12 | plant VAP homolog 12 | chr2:18610806-18613290
FORWARD LENGTH=1262
Length=1262
Score = 237 bits (128), Expect = 6e-62
Identities = 315/402 (78%), Gaps = 25/402 (6%)
Strand=Plus/Plus
Query 5 ACCTTCAATTCCCTTTTGAATTGAAGAAGCAGATGTCTTGCTCTCT-TAACTTGACG-AA 62
|||||||||||||||||||||||||||||||||| ||||| ||||| || ||| | ||
Sbjct 258 ACCTTCAATTCCCTTTTGAATTGAAGAAGCAGATCTCTTGTTCTCTCTAT-TTGG-GTAA 315
Query 63 CAAGACAGACAATAATGGGGCCTTTAAGGTTAAGACGAAAAATCCGAAA-AATTATTGCG 121
|||||| || ||| ||| ||||| ||||||||||||| ||||| ||| || || ||||
Sbjct 316 CAAGACCGATAATTATGTCGCCTTCAAGGTTAAGACGACGAATCC-AAAGAAGTACTGCG 374
Query 122 TTAGGTCTAATTA-TGGACTAATTCTTCCAA-AGTCAACTTGTAAAGTTCTT-GTGACAA 178
||||| ||||| | ||| | ||| ||| | | || ||| | ||||| || ||||| |
Sbjct 375 TTAGGCCTAAT-ACTGGTGTTGTTCATCCCAGA-TCCTCTTCTGAAGTT-TTAGTGACCA 431
Query 179 TGCAAGCCCAAAAGGAAGTTCTTAATTCTGATATGCAGAGCAATGAAAAGTT-TATGATT 237
||||||| |||||||||| || | ||||| ||||| | || || ||||| | || ||
Sbjct 432 TGCAAGCTCAAAAGGAAGCTCCTG---CTGATCTGCAGTGTAAAGATAAGTTCT-TGCTT 487
Query 238 CAAAGTGTAATAGCTAGTCCTGGA-CTCACAGCCAAAGAAGTTACTCGTGAGACGTTAAG 296
||| ||||| | |||||||| ||| | ||| |||| || ||||| | ||||| ||| ||
Sbjct 488 CAATGTGTAGTCGCTAGTCCCGGAGC-CACCCCCAAGGATGTTACACATGAGATGTTTAG 546
Query 297 GAAAGAATCGGGGCATCTAGTTGAGGAGACTAAACTGAAAGTT-TCTTATGTTTGCTCCA 355
||||| | ||||||| |||||| ||||||||| ||| |||| ||| ||||| ||||||
Sbjct 547 CAAAGAGGCAGGGCATCGAGTTGAAGAGACTAAATTGAGAGTTGTCT-ATGTT-GCTCCA 604
Query 356 CCACGACAAC-ATCACCAATTC-ACCAAGGACCAGAAGAGGG 395
||||||| || |||||| ||| | ||||| | ||||||||
Sbjct 605 CCACGACCACCATCACCGGTTCGAG-AAGGATCTGAAGAGGG 645
> AT2G23860.1 | Symbols: | pseudogene, similar to VAP27, blastp
match of 56% identity and 1.3e-25 P-value to GP|6688926|emb|CAB65313.1||AJ251365
VAP27 {Nicotiana plumbaginifolia} |
chr2:10157005-10157669 FORWARD LENGTH=665
Length=665
Score = 172 bits (93), Expect = 2e-42
Identities = 112/121 (93%), Gaps = 2/121 (2%)
Strand=Plus/Plus
Query 298 AAAGAATCGGGGCATCTAGTTGAGGAGACTAAACTGAAAGTTTCTTATGTTTGCTCCACC 357
||||||||||||||||||||||||||||||||||||| |||| |||||||||||||||||
Sbjct 519 AAAGAATCGGGGCATCTAGTTGAGGAGACTAAACTGAGAGTTACTTATGTTTGCTCCACC 578
Query 358 ACGAC-AACATCACCAATTCACCAAGGACCAGAAGAGGGTTTATATTTTAGTGTTTTTGT 416
||||| |||||||||| | ||||||||||||||| | ||||||||||| | |||||||||
Sbjct 579 ACGACCAACATCACCAGTCCACCAAGGACCAGAA-ATGGTTTATATTTCAATGTTTTTGT 637
Query 417 T 417
|
Sbjct 638 T 638
Score = 135 bits (73), Expect = 2e-31
Identities = 109/126 (87%), Gaps = 3/126 (2%)
Strand=Plus/Plus
Query 168 TCTTGTGACAATGCAAGCCCAAAAGGAAGTTC--TTAATTCTGATATGCAGAGCAATGAA 225
||| || || |||||||| |||||||||| | | |||||||||||||||||||||
Sbjct 283 TCTAGTAACCATGCAAGCTCAAAAGGAAGCCCGGCCCA-TCTGATATGCAGAGCAATGAA 341
Query 226 AAGTTTATGATTCAAAGTGTAATAGCTAGTCCTGGACTCACAGCCAAAGAAGTTACTCGT 285
||||||||||||||||| |||| ||||||||||| | ||||||||||||||||||||| |
Sbjct 342 AAGTTTATGATTCAAAGAGTAAAAGCTAGTCCTGCAGTCACAGCCAAAGAAGTTACTCTT 401
Query 286 GAGACG 291
||||||
Sbjct 402 GAGACG 407
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 19755225357
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5