BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg905735
Length=3165
Score E
Sequences producing significant alignments: (Bits) Value
AT3G25500.1 | Symbols: AFH1, FH1, AHF1, ATFH1 | formin homology... 4525 0.0
AT3G25490.1 | Symbols: | Protein kinase family protein | chr3:... 595 7e-169
> AT3G25500.1 | Symbols: AFH1, FH1, AHF1, ATFH1 | formin homology
1 | chr3:9251093-9255002 REVERSE LENGTH=3559
Length=3559
Score = 4525 bits (2450), Expect = 0.0
Identities = 2955/3188 (93%), Gaps = 77/3188 (2%)
Strand=Plus/Plus
Query 11 tcttgttcttgttcttcttctacttactcttatcttcATCCTCCGATCTAGTCTTCGCCG 70
|||| ||||| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 181 TCTTCTTCTTATTCTTCTTCTACTTACTCTTATCTTCATCCTCCGATCTAGTCTTCGCCG 240
Query 71 ACCGTCGTGTCCTTCACGAACCATTCTTCCCTGTAGATTcaccaccaccgtcaccaccat 130
|||||||||| || |||||||||||||||||| |||||||||||||||||||||||||||
Sbjct 241 ACCGTCGTGTACTCCACGAACCATTCTTCCCTATAGATTCACCACCACCGTCACCACCAT 300
Query 131 caccaccaccGCTTCCTAAGCTTCCATTCTCTTCAACTACTCCACCATC-T--TCAGACC 187
|||||||||| |||||||| || |||||||||||||| ||||| ||||| | |||||||
Sbjct 301 CACCACCACCACTTCCTAAACTACCATTCTCTTCAACCACTCCTCCATCTTCATCAGACC 360
Query 188 CAAATGCTTCACCTTTCTTCCCTTTATACCCTTCTTCTCCACCACCACCATCACCAGCCT 247
|||||||||| ||||||||||||||||||||||| |||||||||||||| || |||||||
Sbjct 361 CAAATGCTTCTCCTTTCTTCCCTTTATACCCTTCATCTCCACCACCACCTTCTCCAGCCT 420
Query 248 CATTCGCTTCTTTTCCGGCGAATATCTCATCTCTAATCGTCCCTCACGCCACTAAATCAC 307
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
Sbjct 421 CCTTCGCTTCTTTTCCGGCGAATATCTCATCTCTAATCGTCCCTCACGCCACTAAATCCC 480
Query 308 CACCTAACTCCAAGAAACTCCTTATAGTCGCTATCTCCGCCGTCTCCTCCGCCGCTTTAG 367
||||||||||||| ||||||||||| ||||||||||||||||| |||||||| |||||||
Sbjct 481 CACCTAACTCCAAAAAACTCCTTATCGTCGCTATCTCCGCCGTTTCCTCCGCTGCTTTAG 540
Query 368 TCGCTGTACTTATCGCTTTACTCTATTGGCGTAGAAGAAGAAC-CAACCAAGATCTTAAC 426
||||| ||||||||||||||||||||||||| ||||| | ||| |||||||||||||||
Sbjct 541 TCGCTCTACTTATCGCTTTACTCTATTGGCGAAGAAGCA-AACGTAACCAAGATCTTAAC 599
Query 427 TTCTCCGATGATAGCAAAACTTACACCACCGACAGTAGCCGTAGAGTCTACCCTCCTCCT 486
|||||||||||||||||||| |||||||||||||||||||| | ||||| | ||||||
Sbjct 600 TTCTCCGATGATAGCAAAACATACACCACCGACAGTAGCCGCCGTGTCTA--C-CCTCCT 656
Query 487 CCTCCGGCAACGGCTG-CTCCAACACGACGCAATGCGGAGGCTAGAAGTAAACAGAGGTC 545
|||||||||||||| | ||||||||||||||||||||||||||||||||||||||||| |
Sbjct 657 CCTCCGGCAACGGC-GCCTCCAACACGACGCAATGCGGAGGCTAGAAGTAAACAGAGGAC 715
Query 546 CACGACAAGAACGAGCTCCACCAAT---AACAGCTCTGAGTTTCTTTACTTAGGAACAAT 602
||| | ||||||||||||||| ||||||||||||||||||||||||||||||||
Sbjct 716 CAC--C----ACGAGCTCCACCAATAACAACAGCTCTGAGTTTCTTTACTTAGGAACAAT 769
Query 603 GGTGAATCAAAGAGGAATTGAAGAACAATCTCTTAGTAATAATGGATCAAGCTCAAGAAA 662
|||||||||||||||||| || ||||||||||||||||||||||||||||||||||||||
Sbjct 770 GGTGAATCAAAGAGGAATCGATGAACAATCTCTTAGTAATAATGGATCAAGCTCAAGAAA 829
Query 663 ACTTGAATCTCCAGATCTTCAACCACTTCCTCCATTGATGAAACGAAGTTTCCGGTTAAA 722
|||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
Sbjct 830 ACTTGAATCTCCAGATCTTCAACCACTTCCTCCATTGATGAAACGAAGTTTCCGTTTAAA 889
Query 723 TCCAGATGTTGGTTCAATCGGagaagaagaagaagaagaagaGTTTTACTCTCCACGTGG 782
|||||||||||||||||||||||||||||| |||||||| ||||||||||||||||||||
Sbjct 890 TCCAGATGTTGGTTCAATCGGAGAAGAAGATGAAGAAGATGAGTTTTACTCTCCACGTGG 949
Query 783 CTCACAAAGCGGGCGAGAAACATTAAACCGGGTCGGAGTTCCGGGTCAAAACCCTAGATC 842
||||||||||||||||||| | ||||||||||||||| ||||||||||||| ||||||||
Sbjct 950 CTCACAAAGCGGGCGAGAACCGTTAAACCGGGTCGGACTTCCGGGTCAAAATCCTAGATC 1009
Query 843 TGTTAACAATGACACTATCTCTTGCTCATCTTCAAGCTCTGGTTCACCAGGAAGATCAAC 902
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1010 TGTTAACAATGACACTATCTCTTGCTCATCTTCAAGCTCTGGTTCACCAGGAAGATCAAC 1069
Query 903 GTTTATCAGTATCTCTCCTTCAATGAGTCCTAAAAGATCTGAACCAAAACCTG-TAGTAA 961
|||||||||||||||||||||||||||||||| ||||||||||||||||| | || |
Sbjct 1070 ATTTATCAGTATCTCTCCTTCAATGAGTCCTAAGAGATCTGAACCAAAACC-GCCGGTTA 1128
Query 962 TCACCACACCAGAACCACCGGCAAGGATTTCTCCGGCGGAGTTAACAGATTATAGATTTG 1021
|| | || | |||| | | | |||||||||||||| |||||||||||||
Sbjct 1129 TC-TC-CA-C---ACCA---G--A--A-----CCGGCGGAGTTAACCGATTATAGATTTG 1170
Query 1022 TTAGGTCTCCGTCACTATCGTTAGCTTCTTTATCGTCGGGATTTTGTCCGGTGAAAAACT 1081
|| ||||||||||||| |||||||||||||||||||||||| | |||||||||
Sbjct 1171 TTCGGTCTCCGTCACTGTCGTTAGCTTCTTTATCGTCGGGA-----T----TGAAAAACT 1221
Query 1082 CCGATGAAGGAAGATTGAATCAAATCTCTAGATCTCCGACGGTTACATCACTCACAACTT 1141
||||||||| | ||||||||||||||| ||||||||||||||||||||| || |||||||
Sbjct 1222 CCGATGAAGTAGGATTGAATCAAATCTTTAGATCTCCGACGGTTACATCTCTAACAACTT 1281
Query 1142 CACCGGAGAATAACAAAAAAGAGAACTCTCCGTTATCATCTGCTTCAACTTCACCGGAGA 1201
||||||||||||||||||||||||||||||| ||||||||| |||||||||||||||| |
Sbjct 1282 CACCGGAGAATAACAAAAAAGAGAACTCTCCATTATCATCTACTTCAACTTCACCGGA-A 1340
Query 1202 -GACGACCAAATGATACACCAGAAGCTTATTTGAGATCTCCGTCGCATTCTTCTGCTTCG 1260
|||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
Sbjct 1341 CGACGACCAAATGATACACCAGAAGCTTACTTGAGATCTCCGTCGCATTCTTCTGCTTCT 1400
Query 1261 ACATCACCGTATAGATGTTTTCAGAAGTCTCCGGAGGTGTTACCGGCGTTTATGAGTAAT 1320
|||||||||||||||||||||||||| ||||||||||| |||||||||||||||||||||
Sbjct 1401 ACATCACCGTATAGATGTTTTCAGAAATCTCCGGAGGTCTTACCGGCGTTTATGAGTAAT 1460
Query 1321 CTCCGGCAAGGTTTGCAATCTCAGTTACTTTCTTCTCCTTCAAATTCTCATGGAGGACAA 1380
||||||||||||||||||||||||||||| ||||||||||| || |||||||||||||||
Sbjct 1461 CTCCGGCAAGGTTTGCAATCTCAGTTACTATCTTCTCCTTCTAACTCTCATGGAGGACAA 1520
Query 1381 GGTTTCCTTAAGCAGTTAGATGCATTGCGTTCTCGTTCACCGTCGTCTTCTTCTTCATCA 1440
|||||||||||||||||||||||||| |||||||||||||||||||| |||||||| ||
Sbjct 1521 GGTTTCCTTAAGCAGTTAGATGCATTACGTTCTCGTTCACCGTCGTCGTCTTCTTCTTCT 1580
Query 1441 GTTTGTTCTTCACCGGAGAAAGCTTCTCACAAGTCACCGGTTACATCTCCGAAGTTGTCT 1500
||||||||||||||||||||||||||||| |||||||| ||||||||||| ||||| |||
Sbjct 1581 GTTTGTTCTTCACCGGAGAAAGCTTCTCATAAGTCACCAGTTACATCTCCTAAGTTATCT 1640
Query 1501 TCCCGGAATTCGCAGTCTCTAGCATCATCTCCGGATAGAGATTTTAGTCATAGCTTAGAT 1560
||||||||||||||||||||| |||| |||||||||||||||||||||||||||||||||
Sbjct 1641 TCCCGGAATTCGCAGTCTCTATCATCTTCTCCGGATAGAGATTTTAGTCATAGCTTAGAT 1700
Query 1561 GTATCACCACGGATTTCGAACATTTCACCTCAAATTTTACAGTCTCGAGTGCCTCCGCCT 1620
|||||||||||||| |||||||||||||||||||||||||||||||| ||||||||||||
Sbjct 1701 GTATCACCACGGATATCGAACATTTCACCTCAAATTTTACAGTCTCGTGTGCCTCCGCCT 1760
Query 1621 CCGCCACCTCCTCCA------CCATTGTGGGGACGACGGAATCAGGTGACTCA-TAAGGC 1673
|| || ||||| ||| || |||||||||||||||| |||||||||| | ||| ||
Sbjct 1761 CCTCCTCCTCCCCCACCGTTGCCGTTGTGGGGACGACGGAGTCAGGTGACT-ACTAAAGC 1819
Query 1674 GGAGACAATCTCGAAACCGCCTTCTCTTACACCGCCTTCACATCCATTTGTGATATCATC 1733
||| |||||||||| |||||||||||||||||||||||||||||| |||||||| ||||
Sbjct 1820 GGACACAATCTCGAGACCGCCTTCTCTTACACCGCCTTCACATCCTTTTGTGATCCCATC 1879
Query 1734 CGAAAACTTGCCAGTGAC-T--TCTCCAATGGAGACTCCAGAGACGGTTAGTGCCAGTGA 1790
|||||||| |||||||| | ||||| ||||||||||||||||||||| |||| |||||
Sbjct 1880 TGAAAACTTACCAGTGACTTCGTCTCCTATGGAGACTCCAGAGACGGTTTGTGCGAGTGA 1939
Query 1791 GCCAGCGGATGAAACTCCGAAACCGAAGCTAAAGGCGTTACATTGGGATAAAGTTAGAGC 1850
| | ||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1940 GGCGGCGGAGGAAACTCCGAAACCGAAGCTAAAGGCGTTACATTGGGATAAAGTTAGAGC 1999
Query 1851 AAGTTCGGATCGTGAGATGGTTTGGGATCATCTTCGATCAAGCTCTTTCAAATTAGATGA 1910
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2000 AAGTTCGGATCGTGAGATGGTTTGGGATCATCTTCGATCAAGCTCTTTCAAATTAGATGA 2059
Query 1911 AGAGATGATTGAGACGTTGTTCGTGGCAAAGTCGTTAGATAACAAACCAAATCAGAGTCA 1970
|||||||||||||||||||| ||||| ||||||||| | ||||||||||||||||||||
Sbjct 2060 GGAGATGATTGAGACGTTGTTTGTGGCGAAGTCGTTAAACAACAAACCAAATCAGAGTCA 2119
Query 1971 GACAACTCCAAGATGTGTTCTCCCGAGCCCGAACCAAGAGAACAGAGTCTTGGACCCTAA 2030
||||||||||||||||||||||||||||||||||||||||||||||||| ||||||| ||
Sbjct 2120 GACAACTCCAAGATGTGTTCTCCCGAGCCCGAACCAAGAGAACAGAGTCCTGGACCCGAA 2179
Query 2031 GAAGGCTCAGAATATTGCGATACT-GCTTCGCGCACTTAATGTCACTATAGAAGAAGTTT 2089
|||||||||||||||||| || || |||||| ||||||||||||||||||||||||||||
Sbjct 2180 GAAGGCTCAGAATATTGCCAT-CTTGCTTCGTGCACTTAATGTCACTATAGAAGAAGTTT 2238
Query 2090 GTGAGGCTCTTCTTGAAGGCAATGCTGATACACTTGGGACTGAACTTCTTGAGAGCTTAC 2149
|||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 2239 GTGAGGCTCTTCTTGAAGGCAATGCTGATACACTGGGGACTGAACTTCTTGAGAGCTTAC 2298
Query 2150 TGAAGATGGCACCGACAAAAGAAGAAGAACGCAAATTGAAAGCGTATAAGGATGATTCGC 2209
|||||||||||||||||||||||||||| ||||| ||||||||||| || ||||||||||
Sbjct 2299 TGAAGATGGCACCGACAAAAGAAGAAGAGCGCAAGTTGAAAGCGTACAATGATGATTCGC 2358
Query 2210 CCGTTAAGCTTGGACATGCTGAGAAATTCCTTAAGGCAATGCTGGACATCCCTTTCGCCT 2269
| ||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
Sbjct 2359 CTGTTAAGCTTGGACATGCTGAGAAATTCCTTAAGGCAATGTTGGACATCCCTTTCGCCT 2418
Query 2270 TTAAAAGAGTTGATGCGATGCTCTATGTTGCTAACTTTGAGTCTGAGGTTGAATACCTGA 2329
|||||||||||||||| ||||||||||| || ||||||||||| |||||||||||| |||
Sbjct 2419 TTAAAAGAGTTGATGCAATGCTCTATGTAGCCAACTTTGAGTCCGAGGTTGAATACTTGA 2478
Query 2330 AAAAATCTTTTGAGACTCTTGAGGCTGCTTGTGAGGAACTCAGGAACAGTAGGATGTTCT 2389
| |||||||||||||||||||||||||||||||| ||||| |||||||||||||||||||
Sbjct 2479 AGAAATCTTTTGAGACTCTTGAGGCTGCTTGTGAAGAACTGAGGAACAGTAGGATGTTCT 2538
Query 2390 TAAAGCTT-TTGGAAGCGGTTCTAAAGACCGGAAACCGTATGAACGTTGGAACAAACCGA 2448
|||||||| ||| |||||||||||||||| ||||||||||||||||||||||||||||||
Sbjct 2539 TAAAGCTTCTTG-AAGCGGTTCTAAAGACAGGAAACCGTATGAACGTTGGAACAAACCGA 2597
Query 2449 GGGGATGCTCATGCGTTCAAGCTTGACACACTCCTCAAGCTAGTCGATGTTAAAGGCGCT 2508
|| ||||| ||||||||||||||||| ||||| ||||||||||||||||| |||||||||
Sbjct 2598 GGAGATGCACATGCGTTCAAGCTTGATACACTTCTCAAGCTAGTCGATGTCAAAGGCGCT 2657
Query 2509 GACGGGAAAACAACTCTCTTGCATTTCGTTGTACAAGAGATAATCCGAGCAGAAGGCACA 2568
|| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2658 GATGGGAAAACAACTCTCTTGCATTTCGTTGTACAAGAGATAATCCGAGCAGAAGGCACA 2717
Query 2569 CGTCTCTCAGGTAACAATACA------GATGACATCAAATGCAGGAAACTAGGCCTCCAA 2622
||||||||||||||||||||| |||||||| |||||| |||||||||| ||||||
Sbjct 2718 CGTCTCTCAGGTAACAATACACAAACAGATGACATTAAATGCCGGAAACTAGGTCTCCAA 2777
Query 2623 GTCGTATCAAGTCTCTGTTCAGAGCTAAGTAACGTTAAGAAAGCTGCTGCAATGGACTCA 2682
|| ||||||||||||||||| ||||| |||||||| |||||||||||||| |||||||||
Sbjct 2778 GTTGTATCAAGTCTCTGTTCTGAGCTTAGTAACGTCAAGAAAGCTGCTGCGATGGACTCA 2837
Query 2683 GAAGTACTAAGCAGCTACGTCTCCAAGCTTTCTCAAGGGATCGCCAAGATTAACGAAGCT 2742
|||||||||||||||||||||||||||||||||||||| || |||||||| ||||||||
Sbjct 2838 GAAGTACTAAGCAGCTACGTCTCCAAGCTTTCTCAAGGCATTGCCAAGATCAACGAAGCA 2897
Query 2743 ATCAAAGTCCAATCAACGATGATAGAAGAAAGCAACAGTCAGAGATTTTCGGAATCGATG 2802
||| ||||||||||||| || | ||||||||||||||||||||| |||||||||||||||
Sbjct 2898 ATCCAAGTCCAATCAACAATCACAGAAGAAAGCAACAGTCAGAGGTTTTCGGAATCGATG 2957
Query 2803 AACACGTTTCTGAAAAGAGCTGAGGAAGAGATCATCAGAGTACAAGCTCAAGAGAGCGTC 2862
|| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2958 AAAACGTTTCTGAAAAGAGCTGAGGAAGAGATCATCAGAGTACAAGCTCAAGAGAGCGTA 3017
Query 2863 GCATTATCACTTGTGAAAGAAATCACAGAGTATTTCCACGGAAACTCGGCTAAAGAAGAA 2922
|| ||||||||||| ||||||||||||||||||||||| |||||||||||||||||||||
Sbjct 3018 GCGTTATCACTTGTAAAAGAAATCACAGAGTATTTCCATGGAAACTCGGCTAAAGAAGAA 3077
Query 2923 GCGCACCCGTTTAGAATATTCTTGGTGGTTAGAGACTTCCTTGGAGTAGTAGACAGAGTC 2982
||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3078 GCGCATCCGTTTAGAATATTCTTGGTGGTTAGAGACTTCCTTGGAGTAGTAGACAGAGTT 3137
Query 2983 TGCAAAGAAGTAGGGATGATAAACGAAAGAACAATGGTTAGTTCTGCTCATAAGTTCCCT 3042
|||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
Sbjct 3138 TGCAAAGAAGTAGGGATGATAAACGAAAGAACAATGGTTAGTTCTGCTCATAAGTTTCCT 3197
Query 3043 GTTCCAGTGAATCCAATGCTGCCACAACCTCTTCCTGGACTCGTAGGACGAAGACAAtct 3102
|||||||||||||||||| ||||||||||||||||||||||||| |||||||||||||||
Sbjct 3198 GTTCCAGTGAATCCAATGATGCCACAACCTCTTCCTGGACTCGTTGGACGAAGACAATCT 3257
Query 3103 tcttcttcgtcgtcttcttcttc--CG-CTTCGTCATCA-GACGACGAACATAACTCA-T 3157
|||||||| ||||| || ||||| | ||||||| | | || ||||||||||||||| |
Sbjct 3258 TCTTCTTCTTCGTCGTCGTCTTCAACCTCTTCGTC-TGATGAAGACGAACATAACTCAAT 3316
Query 3158 CTCATTAG 3165
||||||||
Sbjct 3317 CTCATTAG 3324
> AT3G25490.1 | Symbols: | Protein kinase family protein | chr3:9241725-9243485
FORWARD LENGTH=1674
Length=1674
Score = 595 bits (322), Expect = 7e-169
Identities = 353/368 (96%), Gaps = 2/368 (1%)
Strand=Plus/Minus
Query 2747 AAGTCCAATCAACGATGATAGAAGAAAGCAACAGTCAGAGATTTTCGGAATCGAT-GAAC 2805
||||||||||||| || | ||||||||||||||||||||| |||||||||||||| |||
Sbjct 1673 AAGTCCAATCAACAATCACAGAAGAAAGCAACAGTCAGAGGTTTTCGGAATCGATGGAA- 1615
Query 2806 ACGTTTCTGAAAAGAGCTGAGGAAGAGATCATCAGAGTACAAGCTCAAGAGAGCGTCGCA 2865
|||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||
Sbjct 1614 ACGTTTCTGAAAAGAGCTGAGGAAGAGATCATCAGAGTACAAGCTCAAGAGAGCGTAGCG 1555
Query 2866 TTATCACTTGTGAAAGAAATCACAGAGTATTTCCACGGAAACTCGGCTAAAGAAGAAGCG 2925
||||||||||| ||||||||||||||||||||||| ||||||||||||||||||||||||
Sbjct 1554 TTATCACTTGTAAAAGAAATCACAGAGTATTTCCATGGAAACTCGGCTAAAGAAGAAGCG 1495
Query 2926 CACCCGTTTAGAATATTCTTGGTGGTTAGAGACTTCCTTGGAGTAGTAGACAGAGTCTGC 2985
|| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1494 CATCCGTTTAGAATATTCTTGGTGGTTAGAGACTTCCTTGGAGTAGTAGACAGAGTCTGC 1435
Query 2986 AAAGAAGTAGGGATGATAAACGAAAGAACAATGGTTAGTTCTGCTCATAAGTTCCCTGTT 3045
||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||
Sbjct 1434 AAAGAAGTAGGGATGATAAACGAAAGAACAATGGTTAGTTCTGCTCATAAGTTTCCTGTT 1375
Query 3046 CCAGTGAATCCAATGCTGCCACAACCTCTTCCTGGACTCGTAGGACGAAGACAAtcttct 3105
||||||||||||||| ||||||||||||||||||||||||| ||||||| ||||||||||
Sbjct 1374 CCAGTGAATCCAATGATGCCACAACCTCTTCCTGGACTCGTTGGACGAAAACAATCTTCT 1315
Query 3106 tcttcgtc 3113
||||||||
Sbjct 1314 TCTTCGTC 1307
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 157423867134
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5