BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg923562
Length=1519
Score E
Sequences producing significant alignments: (Bits) Value
AT1G50970.1 | Symbols: | Membrane trafficking VPS53 family pro... 741 0.0
AT1G50500.2 | Symbols: HIT1 | Membrane trafficking VPS53 family... 468 8e-131
> AT1G50970.1 | Symbols: | Membrane trafficking VPS53 family protein
| chr1:18896323-18900268 FORWARD LENGTH=1710
Length=1710
Score = 741 bits (401), Expect = 0.0
Identities = 536/595 (90%), Gaps = 34/595 (6%)
Strand=Plus/Plus
Query 1 TATGTAATCATTTCGAAGCATACATGGATGTTCCTAAAATTATGGAGCTCAGAGAAAAAA 60
|||||||||||||| |||||||||||||| |||||||||||||||||||||||||||||
Sbjct 437 TATGTAATCATTTCAAAGCATACATGGATCTTCCTAAAATTATGGAGCTCAGAGAAAAAC 496
Query 61 TTAAGAATATAAAACAAATTTTAAAGTCCCATGTATTTTCTGATTTTTCCAGCTTAGGTA 120
||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||
Sbjct 497 TTAAGAATATAAAACAAATTTTAAAGTTCCATGTATTTTCTGATTTTTCCAGCTTAGGTA 556
Query 121 CTGGGACAAAGACAGAGGAAAtt-tttttGCTAAAGAATTTATCTGATTCTTGCTTGGTT 179
|||||||| ||||||||||| || |||||||||||||| |||||||||||||||||||||
Sbjct 557 CTGGGACAGAGACAGAGGAA-TTGTTTTTGCTAAAGAAGTTATCTGATTCTTGCTTGGTT 615
Query 180 ATTGATGCTCCGGGGCCATCTGTGAGGGAAGAGTTGGTAAACAACTTTTGCAGCAGGGAG 239
||||||||| || |||||||||||||||||||||| |||||||||||||||||||||||
Sbjct 616 GTTGATGCTCTGGAGCCATCTGTGAGGGAAGAGTTGATAAACAACTTTTGCAGCAGGGAG 675
Query 240 CTCACCTCATATGAACAAATTTATGTAGGAGCTGA---G----CG--G-AT-A--TA--- 283
|| |||||||||||||||||||||||||||||||| | || | || | ||
Sbjct 676 CTAACCTCATATGAACAAATTTATGTAGGAGCTGAACTGAAAACGTTGGATGAAATAGAG 735
Query 284 C--A-AT-CGGTTA---AA-TTGTCTAATTCG-AACAAATCAAGAAAAATGGACGATTTT 334
| | || | | | || ||||||||| || || |||||||| ||| |||||||||||
Sbjct 736 CTGATATACAATCAGCTAAGTTGTCTAATCCGCAA-AAATCAAGGAAAGTGGACGATTTT 794
Query 335 TCCTGCTTCATGGCATGTGCCTTATAGACTATGCATCCAGTTGTGCAACAAAACAAGGGT 394
||||||||||||||||||||||||||||||||||||||||||| ||| |||||||||||
Sbjct 795 TCCTGCTTCATGGCATGTGCCTTATAGACTATGCATCCAGTTGAGCAGGAAAACAAGGGT 854
Query 395 GCAAGTAGAATCTATTCTAGTCAACCTGAAAGAGAAGCCAGATGTAGAAAAATTGCTTCT 454
|||||||||||||||||| |||||||||||||||||| ||||||||||||||||||||||
Sbjct 855 GCAAGTAGAATCTATTCTGGTCAACCTGAAAGAGAAGTCAGATGTAGAAAAATTGCTTCT 914
Query 455 GGAACTGAAAAGGACGCTAGAGTTTGAAATGGAACTTGAAATGAAATTTGTTGGTGGTGG 514
||||||||||||||||||||||||||||| |||||||||||||||||||| |||||||||
Sbjct 915 GGAACTGAAAAGGACGCTAGAGTTTGAAAGGGAACTTGAAATGAAATTTGGTGGTGGTGG 974
Query 515 GAGTTTCGGAGATGACACTAAGGAAATTGGTGGAGGGGGAAATAATAGTCAGAAA 569
|||| |||||||||||| ||| |||||||||||||||||||||||||||||
Sbjct 975 GAGTATCGGAGATGACA-TAA-----TTGGTGGAGGGGGAAATAATAGTCAGAAA 1023
Score = 372 bits (201), Expect = 6e-102
Identities = 361/432 (84%), Gaps = 36/432 (8%)
Strand=Plus/Plus
Query 1104 ACTCCAACTGCGAGCTATGTCGAATTTGTGAACCATCAAATGAAAAGAGCTGAAGCAGTC 1163
||| ||||||||||||||||| || |||||||||||||||||||||||||||||||||||
Sbjct 1279 ACTGCAACTGCGAGCTATGTCAAACTTGTGAACCATCAAATGAAAAGAGCTGAAGCAGTC 1338
Query 1164 CTAAAGGTCATAACATCCCCAATCGTCTCAGTGGTAGACACTTATTGTGCACTGTTTCCA 1223
|||||||||||| |||||||||||||| ||||| ||||||||||| ||||||||||||||
Sbjct 1339 CTAAAGGTCATAGCATCCCCAATCGTCACAGTGATAGACACTTATCGTGCACTGTTTCCA 1398
Query 1224 GAAGGAACACCAATGGAGTTTCAACGCATCTTGGAACTTAAG---CT---GA--GC--AG 1273
|||| ||||||||||||||||||||||||||||| ||||||| || || || ||
Sbjct 1399 GAAGAAACACCAATGGAGTTTCAACGCATCTTGGTACTTAAGGGTCTTACGAAAGCTGAG 1458
Query 1274 -A--AGAGTATACTTGATGATTTCAACAATC----CT---G---------------G-TT 1307
| ||||||||||||||||||||||||||| || | | ||
Sbjct 1459 CAGCAGAGTATACTTGATGATTTCAACAATCATAGCTCAAGAATCACACAACTCTCGGTT 1518
Query 1308 GCAGCGGCAATGCCAGAAGCACCTGCACCGCCACTGGCAGTCGCTAATCCGGGTGCAGCT 1367
|||||| || ||||||||||| ||||| |||||| ||| || ||||| || | |||||
Sbjct 1519 GCAGCGAAAACGCCAGAAGCACATGCACTGCCACTTGCACTCACTAATGTGGCTCCAGCT 1578
Query 1368 GTTGGGTTCATAGCAAACAGTGAGGAAGTGTTAACCACAGCCGCTGAAGCTGCAACCACT 1427
|| |||| | |||||||||||||||||||||||||| ||| ||| |||||||||||||
Sbjct 1579 GTCCGGTTTAAAGCAAACAGTGAGGAAGTGTTAACCAGAGCAGCTTCAGCTGCAACCACT 1638
Query 1428 AGCTTCATGAAGTTATATTCTGTTACTGAAACGGCCAAAGACCGTCCCTTCAGGAAACTC 1487
|||||||||||||||||| || |||||| | | |||||||||||||||||||||||||||
Sbjct 1639 AGCTTCATGAAGTTATATGCTCTTACTGGAGCTGCCAAAGACCGTCCCTTCAGGAAACTC 1698
Query 1488 TTCAATGCATGA 1499
|||||| |||||
Sbjct 1699 TTCAATCCATGA 1710
Score = 100 bits (54), Expect = 3e-20
Identities = 60/63 (95%), Gaps = 0/63 (0%)
Strand=Plus/Plus
Query 1041 ATGTTGCTAGATGCGCACGATATGAAGATGATAGTTTTAAAAGTTCCATCTCTTGCCAGA 1100
||||||||||||||||| |||||||||||||| ||||||||||||||||||||||||||
Sbjct 1189 ATGTTGCTAGATGCGCATGATATGAAGATGATTCTTTTAAAAGTTCCATCTCTTGCCAGA 1248
Query 1101 CAG 1103
|||
Sbjct 1249 CAG 1251
> AT1G50500.2 | Symbols: HIT1 | Membrane trafficking VPS53 family
protein | chr1:18707990-18715935 REVERSE LENGTH=2907
Length=2907
Score = 468 bits (253), Expect = 8e-131
Identities = 451/541 (83%), Gaps = 36/541 (7%)
Strand=Plus/Plus
Query 3 TGTAATCA-TTTCGAAGCATACATGGATGTTCCTAAAATTATGGAGCTCAGAGAAAAAAT 61
||||| || ||| |||||||||| ||||||||||||||| | |||||||||||| || |
Sbjct 632 TGTAACCACTTT-GAAGCATACAGGGATGTTCCTAAAATCACGGAGCTCAGAGAGAAGCT 690
Query 62 TAAGAATATAAAACAAAT-TTTAAAGTCCCATGTATTTTCTGATTTTTCCAGCTTAGGTA 120
||| |||||||| ||||| || ||||||||||||||||||||||||||||| |||||||
Sbjct 691 TAATAATATAAAGCAAATCCTT-AAGTCCCATGTATTTTCTGATTTTTCCAGTTTAGGTA 749
Query 121 CTGGGACAA-AGACAGAGGAAAtttttttGCTAAAGAATTTATCTGATTCTTGCTTGGTT 179
|||||| || |||||||||||| ||||||| |||| |||||||||||||||||||||
Sbjct 750 CTGGGA-AAGAGACAGAGGAAACAAATTTGCTACAGAAGTTATCTGATTCTTGCTTGGTT 808
Query 180 ATTGATGCTCCGGGGCCATCTGTGAGGGAAGAGTTGGTAAACAACTTTTGCAGCAGGGAG 239
||||||||| || |||||||||||||||||||||||||| ||||||||||||||||||
Sbjct 809 GTTGATGCTCTGGAACCATCTGTGAGGGAAGAGTTGGTAAATAACTTTTGCAGCAGGGAG 868
Query 240 CTCACCTCATATGAACAAATTTATGTAGGAGCTGA---G-C---G--G-ATAT-ACA-AT 287
|||||||||||||||||||||| || ||||||||| | | | | ||| ||| |
Sbjct 869 CTCACCTCATATGAACAAATTTTTGAAGGAGCTGAATTGGCAAAGTTGGATAAGACAGAG 928
Query 288 CGGTTAAAT--T-G--T----C-T--AATTCGAACAAATCAAGAAAAATGGACGATTTTT 335
||| | || | | | | | ||| ||||||||| |||||| |||| |||||||
Sbjct 929 CGGAGATATGCTTGGATAAAGCGTCGAATCCGAACAAATGAAGAAATTTGGAAGATTTTT 988
Query 336 CCTGCTTCATGGCATGTGCCTTATAGACTATGCATCCAGTTGTGCAA-CAAAACAAGGGT 394
|||||||| ||||||||||| ||||| |||||||| ||||| ||||| |||| |||||
Sbjct 989 CCTGCTTCTTGGCATGTGCCGTATAGGCTATGCATTCAGTTCTGCAAGCAAA-CAAGGAA 1047
Query 395 GCAAGTAGAATCTATTCTAGTCAACCTGAAAGAGAAGCCAGATGTAGAAAAATTGCTTCT 454
|||||||||||||||||| |||||| ||||||||||||||| ||||| | |||||||||
Sbjct 1048 GCAAGTAGAATCTATTCTGGTCAACATGAAAGAGAAGCCAGTTGTAGCGATATTGCTTCT 1107
Query 455 GGAACTGA-AAAGGAC-GCTAGAGTTTGAAATGGAACTTGAAATGAAATTTGTTGGTGGT 512
|| ||| | |||| || | | |||||||||| |||||||||| |||||||| |||||||
Sbjct 1108 GGCACT-ACAAAGTACAG-TGGAGTTTGAAAAAGAACTTGAAAAGAAATTTGGTGGTGGT 1165
Query 513 G 513
|
Sbjct 1166 G 1166
Score = 446 bits (241), Expect = 4e-124
Identities = 346/396 (87%), Gaps = 10/396 (3%)
Strand=Plus/Plus
Query 572 GGAGGAAAT-ATGGGATATTGAAGA-GGAAAGTCAAACTAATATTCTATCTAGCAGCATA 629
||||| ||| ||||||| |||| || || ||| |||| |||| || ||||||| |||| |
Sbjct 1453 GGAGG-AATCATGGGATGTTGAGGATGG-AAGCCAAAATAATGTTTTATCTAGTAGCACA 1510
Query 630 CTGTTGTTTTTCACTATAAAAAAGAGCTTGAAACGTTGTAGTGCTCTCACCAAGAACCAG 689
| | | |||| || |||||| |||||||||||||| |||| | |||||| |||||||||
Sbjct 1511 CAGCTATTTTCCAATATAAAGAAGAGCTTGAAACGCTGTAATACTCTCAGTAAGAACCAG 1570
Query 690 ACTTTATTCAATTTGTTCAAGGTCTTTCAACGAGTTCTGAAAG-CCTATGCAACAAAGCT 748
|||||||||||||||||||||||||||||||||||| | |||| ||||||| ||||||||
Sbjct 1571 ACTTTATTCAATTTGTTCAAGGTCTTTCAACGAGTTTT-AAAGGCCTATGCTACAAAGCT 1629
Query 749 ATGTTTTAAGCTCCCAAAAGGAGGCACCAGTATCATTGCCGCAGCCACAGGCATGGAGGG 808
|| |||||||||||||||||||||||| ||||| |||||||||||||||||||||| ||
Sbjct 1630 ATTTTTTAAGCTCCCAAAAGGAGGCACTGGTATCGTTGCCGCAGCCACAGGCATGGATGG 1689
Query 809 TCGGATAAAGGTCTCGGACAAAGATGAAAGGATGATATGTTACATAGTTAATACTGCAGA 868
| ||||||||||||||| | |||||||||| ||||||| |||||||||||| |||| ||
Sbjct 1690 ACAGATAAAGGTCTCGGAGAGAGATGAAAGGGTGATATGCTACATAGTTAATTCTGCTGA 1749
Query 869 GTATTGTCACAAAACATCTGGTGATCTGGCAGAAGAA-GTCTCA-ACAATTATTGACCCG 926
|||||| ||||||||||||||||| ||||||||| || |||||| | ||||||||||||
Sbjct 1750 GTATTGCCACAAAACATCTGGTGAACTGGCAGAA-AACGTCTCAGA-AATTATTGACCCA 1807
Query 927 CCTTATGCTGACGGTGTAGACATATCAGAGGTCCAG 962
| ||||||||| ||||||||||| ||||||||||||
Sbjct 1808 CATTATGCTGATGGTGTAGACATGTCAGAGGTCCAG 1843
Score = 311 bits (168), Expect = 1e-83
Identities = 267/313 (85%), Gaps = 13/313 (4%)
Strand=Plus/Plus
Query 961 AGCTGGCATTATCCCTTGGACCAAGGTTCTATGCTAATATTTTCAGATGCAAGCAAATAT 1020
||||||||| |||||| |||||| |||||||||||||||||||||||||||||||| |||
Sbjct 2070 AGCTGGCATCATCCCTAGGACCACGGTTCTATGCTAATATTTTCAGATGCAAGCAACTAT 2129
Query 1021 CAGAAACTGGAGCTCACCAGATGTTGCTAGATGCGCACGATATGAAGAT-GATAGTTTTA 1079
| |||||||||||||| |||||||| |||||| |||| | | ||||| | ||| | ||
Sbjct 2130 CCGAAACTGGAGCTCAACAGATGTTACTAGATACGCAAGCTGTGAAG-TCGATTCTACTA 2188
Query 1080 -AAAGTTCCATCTCTTGCCAGACAGAC-TCCAACTGC-G--AGCTATGTCGAA-TTTGTG 1133
||| |||| || |||||||||||||| || |||||| | |||||| || || ||||||
Sbjct 2189 GAAA-TTCCTTCCCTTGCCAGACAGACCTC-AACTGCTGCCAGCTAT-TCCAAATTTGTG 2245
Query 1134 AACCATCAAATGAAAAGAGCTGAAGCAGTCCTAAAGGTCATAAC-ATCCCCAATCGTCTC 1192
| || | |||||| |||||||||||| |||| ||||||||| | ||||||||||| |||
Sbjct 2246 AGCCGTGAAATGAGCAGAGCTGAAGCACTCCTTAAGGTCATA-CTATCCCCAATCGACTC 2304
Query 1193 AGTGGTAGACACTTATTGTGCACTGTTTCCAGAAGGAACACCAATGGAGTTTCAACGCAT 1252
||||| |||||||||| ||||||||||||| || ||||| || |||||||||||||||||
Sbjct 2305 AGTGGCAGACACTTATCGTGCACTGTTTCCGGAGGGAACGCCCATGGAGTTTCAACGCAT 2364
Query 1253 CTTGGAACTTAAG 1265
||| |||||||||
Sbjct 2365 CTTAGAACTTAAG 2377
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 74949413685
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5