BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg924955
Length=287
Score E
Sequences producing significant alignments: (Bits) Value
AT1G60913.1 | Symbols: | Rapid alkalinization factor (RALF) fa... 268 2e-71
AT1G60835.1 | Symbols: | Encodes a Rapid ALkalinization Factor... 128 3e-29
AT1G60625.1 | Symbols: RALFL6 | RALF-like 6 | chr1:22333916-223... 111 3e-24
> AT1G60913.1 | Symbols: | Rapid alkalinization factor (RALF)
family protein | chr1:22428605-22428844 REVERSE LENGTH=240
Length=240
Score = 268 bits (145), Expect = 2e-71
Identities = 217/249 (87%), Gaps = 15/249 (6%)
Strand=Plus/Plus
Query 25 ATGGCTACACACAAAATGAGTCTTAC-CGTTC-TTTCTTTTGTTTCAATGA-TGATTATA 81
|||||| ||||||||||||||||||| | || |||| ||||| ||||| | ||||| ||
Sbjct 1 ATGGCTGCACACAAAATGAGTCTTACTAG-TCTTTTC-TTTGTGTCAAT-AGTGATTGTA 57
Query 82 TTATCATTGTTCAGCGGATTCGGAGAAGGAAGAAA-ATATTTGAAATATGGAGTTATTAC 140
||||||||||||||||||||||||||||||||| | ||| |||||| ||| |||| |
Sbjct 58 TTATCATTGTTCAGCGGATTCGGAGAAGGAAGATACATA----AAATATAGAGCTATTGC 113
Query 141 AAAAGACAGAATTCCAAACTGC-CGTCA-GAATCCCGAAAATTGTGTCAGAGTTCCAGCC 198
|||||| ||| ||||| ||||| | ||| || |||| ||||||||||||||||||||| |
Sbjct 114 AAAAGATAGAGTTCCAGACTGCAC-TCAAGA-TCCCAAAAATTGTGTCAGAGTTCCAGTC 171
Query 199 AACCAATACCATCTTCCTCCTGGTTGTAAAAATTCTACACATTGTTATCGTGAAAAGTAT 258
||||| ||||||||||||||||||||| |||| ||||||||||||||||||||||||||
Sbjct 172 AACCAGTACCATCTTCCTCCTGGTTGTCAAAACACTACACATTGTTATCGTGAAAAGTAT 231
Query 259 CATATTTAA 267
|||||||||
Sbjct 232 CATATTTAA 240
> AT1G60835.1 | Symbols: | Encodes a Rapid ALkalinization Factor
(RALF) family protein [pseudogene] | chr1:22396666-22396913
FORWARD LENGTH=248
Length=248
Score = 128 bits (69), Expect = 3e-29
Identities = 149/185 (81%), Gaps = 15/185 (8%)
Strand=Plus/Plus
Query 37 AAAATGAGTCTTA-CCGTTC-TTTCTTTTGTTTCAATGA-TGATTATATTATCATTGTTC 93
|||| |||||||| ||| || |||| ||||||||| | | ||||||||||||||||||||
Sbjct 13 AAAAAGAGTCTTACCCG-TCTTTTC-TTTGTTTCAGTAATTGATTATATTATCATTGTTC 70
Query 94 AGCGGATTCGGAGAAGGAAGAAAATATTTGAAA-TATGGAGTTATTACAAAAGACAGAAT 152
|||||||| ||||||||||| |||||| | ||| ||| || ||| | ||||||||||||
Sbjct 71 AGCGGATTAGGAGAAGGAAGCAAATATAT-AAACTATAAAGATATGATAAAAGACAGAAT 129
Query 153 TCCAAACT-GCCGTCAG-AAT--CCCG-AAA-ATTGTGTCAGAGTTCCAGCCAACCAATA 206
|||| || || | ||| || ||| ||| | ||||||| | |||||| ||||| ||
Sbjct 130 TCCAG-CTTGCGG-CAGTAAAAGCCCCCAAAGAATGTGTCAAATTTCCAGTCAACCTTTA 187
Query 207 CCATC 211
|||||
Sbjct 188 CCATC 192
> AT1G60625.1 | Symbols: RALFL6 | RALF-like 6 | chr1:22333916-22334161
FORWARD LENGTH=246
Length=246
Score = 111 bits (60), Expect = 3e-24
Identities = 145/183 (79%), Gaps = 17/183 (9%)
Strand=Plus/Plus
Query 25 ATGGCTACACACAAAATGAGTCTTA-CCG-TTCTTTCTTTTGTTTCAATGATGATTATAT 82
|||||| ||||||||| ||||| || ||| | |||| ||||||||| | ||||||||||
Sbjct 1 ATGGCTGCACACAAAAAGAGTCATATCCGCAT-TTTC-TTTGTTTCAGTAATGATTATAT 58
Query 83 TATCATTGTTCAGCGGATTCGGAGAAGGAAGAAA-ATATTTGAAA-TATGGA-GTTATTA 139
||||||||||||||||||| ||||||||| ||| |||| | ||| ||| | | ||| |
Sbjct 59 TATCATTGTTCAGCGGATTTGGAGAAGGAC-AAACATATAT-AAACTATA-ATGGTATGA 115
Query 140 CAAA-AGACAGAATTCCAAACTGC--C-GTCAGAATCCCGAA-AATTGTGTCAGAGTTCC 194
|| ||||| ||||||| ||| | || |||||||| || ||| |||||| | ||||
Sbjct 116 -AAGGAGACATAATTCCAGGTTGCAGCAGTAAGAATCCCAAAGAAT-GTGTCAAAATTCC 173
Query 195 AGC 197
|||
Sbjct 174 AGC 176
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 13220417987
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5