BLASTN 2.2.26+ Reference: Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000), "A greedy algorithm for aligning DNA sequences", J Comput Biol 2000; 7(1-2):203-14. Database: TAIR10_cdna_20110103_representative_gene_model_updated 33,602 sequences; 51,074,197 total letters Query= Ahg934181 Length=1294 Score E Sequences producing significant alignments: (Bits) Value AT1G31650.1 | Symbols: ATROPGEF14, ROPGEF14 | RHO guanyl-nucleo... 455 5e-127 > AT1G31650.1 | Symbols: ATROPGEF14, ROPGEF14 | RHO guanyl-nucleotide exchange factor 14 | chr1:11326301-11330738 REVERSE LENGTH=2255 Length=2255 Score = 455 bits (246), Expect = 5e-127 Identities = 409/486 (84%), Gaps = 18/486 (4%) Strand=Plus/Plus Query 236 ACATTTAT-ATGGTAAGGAGAAACGTGGCTA--TGCACTCTGCTTTTCTGACGTTGAAGT 292 |||||| | ||| ||||||||||| || || || ||| | | ||| || || |||| Sbjct 711 ACATTT-TGATGCTAAGGAGAAACCAGGGTATGTGTACTGT-CATTT-GGATGTGGAAG- 766 Query 293 C-ATGAAGGAGAGATTTTCAAAGCTATTTCTCGGTGAAGATGTTAC-GGGAGGATGCAAC 350 | |||||||||| |||||| ||| |||| ||||| |||||||| || ||| ||||| || Sbjct 767 CTATGAAGGAGAAATTTTCTAAGTTATTGCTCGGCGAAGATGTCACTGGG-GGATGTAAG 825 Query 351 GGTGTGCAAACCGCTTTGGCTTTATCAAACGCTATAACTCATCTTGCTACCTCGGTTTTT 410 ||||| ||| ||||||||||| ||||| ||| |||| ||||||||||| ||| ||||| Sbjct 826 GGTGTCCAAGTAGCTTTGGCTTTGTCAAATGCTGTAACACATCTTGCTACGTCGATTTTT 885 Query 411 GGCGAGCTCTGGAAGTTGGAACCATTGTGTGAAGACAA-AAAGCAGAGATGGAGAACGGA 469 |||||||||||||| |||||||| ||||||||||| || ||| |||| |||||||| ||| Sbjct 886 GGCGAGCTCTGGAAATTGGAACCTTTGTGTGAAGAGAAGAAA-CAGAAATGGAGAAGGGA 944 Query 470 AATGGATTGGTTACTCTCTCCAACTAACTACATGATTGAGTTAGTCCCATCTAAGCAAGA 529 |||||||||| |||| |||||||||||||||||||||||||||||||| ||||||||| | Sbjct 945 AATGGATTGGCTACTTTCTCCAACTAACTACATGATTGAGTTAGTCCCTTCTAAGCAAAA 1004 Query 530 TGGCGAAAACGGAAGATCACTTGAGATAATGACGCCGAAAGCCCGTGCAGACATTCATAT 589 || | || ||||||||||||||||||||||| ||||||||||| |||||||||||||| Sbjct 1005 TGATGCCAATGGAAGATCACTTGAGATAATGACTCCGAAAGCCCGCGCAGACATTCATAT 1064 Query 590 GAATATACCAGCTCTCCGGAAATTGGACTCCATGCTAATTGAGACACTGGACTCTGTGGT 649 |||| | |||||||| | ||| |||||||| |||||||||||||||||||| ||| |||| Sbjct 1065 GAATCTCCCAGCTCTTCAGAAGTTGGACTCAATGCTAATTGAGACACTGGATTCTATGGT 1124 Query 650 AAACACAGAGTTTTGGTATTCAGA-AGTGGGTCACAAG-GCAGA-GGGAAAGAACAAGAT 706 |||||||||||||||||||| || | ||| | | || || || ||||| |||||||| Sbjct 1125 GAACACAGAGTTTTGGTATTCTGAGATTGG-T-AGTAGAGCTGAAGGGAA-GAACAAGAG 1181 Query 707 TACAAG 712 |||||| Sbjct 1182 TACAAG 1187 Score = 139 bits (75), Expect = 6e-32 Identities = 145/178 (81%), Gaps = 8/178 (4%) Strand=Plus/Plus Query 17 AATAATGACTTATGATGGCTTAGAAACTTATATCATCAACAATCAATCTTTA-GAAGAAG 75 ||| || || ||| ||||||| ||||||| ||| ||||| |||||||| || ||||||| Sbjct 435 AATGATTACATATTATGGCTTGGAAACTTGTATTATCAATAATCAATC-ATATGAAGAAG 493 Query 76 AAAGTGGAACAAGCCGCGGAGAT-GAGTACCCGA-ATGATTCATTGGATGATGATTCATT 133 | ||||||||||| || |||||| || | | | | ||||| || ||||||||| |||| Sbjct 494 AGAGTGGAACAAGTCGAGGAGATGGA-TGTCTTACA-GATTCGTTAGATGATGATGCATT 551 Query 134 TTCAAGCTGTTCATCAAGCAAAGATGTGCTCGAG-TCATTTTCCTCAAAATTGTTACC 190 |||||||||||||||||||||||||| ||| || || ||||| || |||| |||||| Sbjct 552 TTCAAGCTGTTCATCAAGCAAAGATGC-CTCTAGCTCTTTTTCTTCGAAATGGTTACC 608 Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 63654291060 Database: TAIR10_cdna_20110103_representative_gene_model_updated Posted date: Sep 25, 2014 6:13 PM Number of letters in database: 51,074,197 Number of sequences in database: 33,602 Matrix: blastn matrix 1 -2 Gap Penalties: Existence: 0, Extension: 2.5