BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg937469
Length=692
Score E
Sequences producing significant alignments: (Bits) Value
AT3G47350.2 | Symbols: ATHSD2, HSD2 | hydroxysteroid dehydrogen... 497 4e-140
AT3G47360.1 | Symbols: ATHSD3, HSD3 | hydroxysteroid dehydrogen... 189 3e-47
> AT3G47350.2 | Symbols: ATHSD2, HSD2 | hydroxysteroid dehydrogenase
2 | chr3:17446818-17449703 FORWARD LENGTH=996
Length=996
Score = 497 bits (269), Expect = 4e-140
Identities = 312/332 (94%), Gaps = 5/332 (2%)
Strand=Plus/Plus
Query 204 GGATATTAACTTTTGGGGCTCAACGTACATCACTTATTTTGCTATTCCTCATCTTCGAAA 263
|||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 489 GGATATCAACTTTTGGGGCTCAACGTACATCACTTATTTTGCTATTCCTCATCTTCGAAA 548
Query 264 AAGCAAAGGAAAAATTGTTGTGATTACTTCTGCTACGGCAATTATACCTTTGCCATCGGC 323
||||||||||||||||||||||||| ||||||||||||||||||||||||||| | ||||
Sbjct 549 AAGCAAAGGAAAAATTGTTGTGATTTCTTCTGCTACGGCAATTATACCTTTGCAAGCGGC 608
Query 324 AAGCGTCTATTCAGCAAGCAAAGCAGCTTTATTAAAATTCTTTGAAACATTAAGAGTTGA 383
||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||
Sbjct 609 AAGCGTCTATTCAGCAAGCAAAGCAGCTTTAGTAAAATTCTTTGAAACATTAAGAGTTGA 668
Query 384 GATCAGTCCATG-TATCAAAATAACAATTGCTATTCCCGAATTTATATCTACAGATATGA 442
||||||||| | ||||||||||||||| ||| |||||| |||||||||||| |||||||
Sbjct 669 GATCAGTCCC-GATATCAAAATAACAATCGCTCTTCCCGGATTTATATCTACGGATATGA 727
Query 443 CCACTC---AGTTTAAAGAAATGTATGGTTCAGATTTCATCCTTTCAGAATCAGTGAGTA 499
|||||| ||||||||||||||||||||||||||||||| ||||| ||||||||||||
Sbjct 728 CCACTCCTCAGTTTAAAGAAATGTATGGTTCAGATTTCATACTTTCGGAATCAGTGAGTC 787
Query 500 AATGCGCAAAAGCCATCTTCAGAGGTATTGGT 531
|||||||||||| ||||||||||||||||||
Sbjct 788 GATGCGCAAAAGCGATCTTCAGAGGTATTGGT 819
Score = 219 bits (118), Expect = 4e-56
Identities = 164/186 (88%), Gaps = 3/186 (2%)
Strand=Plus/Plus
Query 19 AACATGGATAAGCTTCACAATATTCTTAATCTTCTTCTACCTCCTCTTACCATCAGTTTT 78
|||||||||| |||||||| |||||| ||| ||||||| ||||||||||| |||||||||
Sbjct 28 AACATGGATATGCTTCACACTATTCTCAATTTTCTTCTTCCTCCTCTTACTATCAGTTTT 87
Query 79 CTTGTCTTCTTTTACCCATTTTATCTTTTGATCAAACTTGTGAGTTGTCTTTACAAGAAC 138
|| ||| | |||||||||||||||||||| | ||||||| ||||||||| || ||| ||
Sbjct 88 CTCGTCCTATTTTACCCATTTTATCTTTTTACCAAACTTATGAGTTGTC-TT--AAGCAC 144
Query 139 ATTCGCTTTGAAAACGTTGCCGGAAAAGTAGTTCTAATCACCGGAGCTTCCTCCGGCATT 198
||| ||||||||||||| | || |||||||||||||||||||||||||||||||||||
Sbjct 145 CTTCACTTTGAAAACGTTACTGGGAAAGTAGTTCTAATCACCGGAGCTTCCTCCGGCATC 204
Query 199 GGAGAG 204
||||||
Sbjct 205 GGAGAG 210
> AT3G47360.1 | Symbols: ATHSD3, HSD3 | hydroxysteroid dehydrogenase
3 | chr3:17451112-17452843 FORWARD LENGTH=930
Length=930
Score = 189 bits (102), Expect = 3e-47
Identities = 146/167 (87%), Gaps = 4/167 (2%)
Strand=Plus/Plus
Query 40 ATTCTTAATCTTCTTCTACCTCCTCTTACCATCAGT-TTTCT-TGTCTTCTTTTACCCAT 97
||||| ||||||||||| ||||||||||| |||| | ||||| | || | ||||||||||
Sbjct 19 ATTCTCAATCTTCTTCTTCCTCCTCTTACTATCA-TCTTTCTCT-TCCTGTTTTACCCAT 76
Query 98 TTTATCTTTTGATCAAACTTGTGAGTTGTCTTTACAAGAACATTCGCTTTGAAAACGTTG 157
||||||||||||||||||||||| ||||||| |||||| ||| ||| |||||||| |
Sbjct 77 TTTATCTTTTGATCAAACTTGTGCTTTGTCTTCGTAAGAACCTTCACTTCGAAAACGTAG 136
Query 158 CCGGAAAAGTAGTTCTAATCACCGGAGCTTCCTCCGGCATTGGAGAG 204
|| ||||||||||||||||||||||||||||||| ||||| ||||||
Sbjct 137 CCAGAAAAGTAGTTCTAATCACCGGAGCTTCCTCTGGCATCGGAGAG 183
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 33506176049
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5