BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg939524
Length=347
Score E
Sequences producing significant alignments: (Bits) Value
AT4G04720.1 | Symbols: CPK21 | calcium-dependent protein kinase... 422 1e-117
AT4G04740.2 | Symbols: CPK23 | calcium-dependent protein kinase... 185 2e-46
> AT4G04720.1 | Symbols: CPK21 | calcium-dependent protein kinase
21 | chr4:2394458-2397759 REVERSE LENGTH=2083
Length=2083
Score = 422 bits (228), Expect = 1e-117
Identities = 311/349 (89%), Gaps = 13/349 (4%)
Strand=Plus/Plus
Query 1 GAAAGTCTCCTCCTTTATTAATGGGTTGCGTT-AGTAGTAAACACCGGAAAACTCAAAAC 59
||||||||||||||||| ||||||||||| || || ||||||||||||||||||||||||
Sbjct 109 GAAAGTCTCCTCCTTTACTAATGGGTTGC-TTCAGCAGTAAACACCGGAAAACTCAAAAC 167
Query 60 GACGGTGGAGAAAGAA-CAATTCCGATCAATCCAGTACAAACCCATGTCGTTAATCAAGT 118
||||| ||||||| || |||| |||||||||||||| |||||||| | | | |||
Sbjct 168 GACGGCGGAGAAA-AATCAATCCCGATCAATCCAGTTCAAACCCA--T-G----T--AGT 217
Query 119 CCCTGAGCATTGTAAACCTCAAATCCTAACACCCAAACCCATGAAACAAGCAATCCTTCA 178
||| |||||| |||||||||||| || |||||| |||||||||| ||| |||||| |||
Sbjct 218 CCCCGAGCATCGTAAACCTCAAACCCCAACACCAAAACCCATGACTCAACCAATCCATCA 277
Query 179 ACAGATTTCAACGCCGTCTTCAAACCCAATCTCAGTTCGAGATCCAGATACGATTTTAGG 238
||||||||||| ||||||||||| ||| |||||||||||||||||||||||||||||||
Sbjct 278 GCAGATTTCAACACCGTCTTCAAATCCAGTCTCAGTTCGAGATCCAGATACGATTTTAGG 337
Query 239 TAAACCATTCGAAGACATCAGGAAATCTTACAACTTGGGAAAAGAATTAGGTCGAGGTCA 298
|||||||||||||||||||||||||| ||||| |||||| ||||||||||||||||||||
Sbjct 338 TAAACCATTCGAAGACATCAGGAAATTTTACAGCTTGGGGAAAGAATTAGGTCGAGGTCA 397
Query 299 ATTTGGGATTATGTATATGTGTAAATAGATTGGAACTGGGAACACTTAT 347
||||||||||| ||||||||||||| ||||||| ||||| |||||||||
Sbjct 398 ATTTGGGATTACGTATATGTGTAAAGAGATTGGTACTGGTAACACTTAT 446
> AT4G04740.2 | Symbols: CPK23 | calcium-dependent protein kinase
23 | chr4:2403609-2408737 REVERSE LENGTH=2518
Length=2518
Score = 185 bits (100), Expect = 2e-46
Identities = 132/147 (90%), Gaps = 3/147 (2%)
Strand=Plus/Plus
Query 202 ACCCAATCTCAGTTCGAGATCCAGATACGATTTTAGGTAAACCATTCGAAGACATCAGGA 261
|||| |||||||||||||||||||| ||||||||||||||||||||||||||||||||||
Sbjct 385 ACCC-ATCTCAGTTCGAGATCCAGAGACGATTTTAGGTAAACCATTCGAAGACATCAGGA 443
Query 262 AATCTTACAACTTGGGAAAAGAATTAGGTCGAGGTCAATTT-GGGATTATGTATATGTGT 320
||| ||||| |||||| | |||||||||||||||| ||| ||||||| |||||||||
Sbjct 444 AATTTTACAGCTTGGGGAGAGAATTAGGTCGAGGTGG-TTTAGGGATTACGTATATGTGC 502
Query 321 AAATAGATTGGAACTGGGAACACTTAT 347
||| ||||||| |||||||||| ||||
Sbjct 503 AAAGAGATTGGGACTGGGAACATTTAT 529
Score = 169 bits (91), Expect = 2e-41
Identities = 135/155 (87%), Gaps = 7/155 (5%)
Strand=Plus/Plus
Query 2 AAAGTCTCCTCCTTTATTAATGGGTTGCGTT-AGTAGTAAACACCGGAAAACTCAAAACG 60
|||||||||||||||| |||||||||| || || |||||||||||||||||||||||||
Sbjct 226 AAAGTCTCCTCCTTTACTAATGGGTTG-TTTCAGCAGTAAACACCGGAAAACTCAAAACG 284
Query 61 ACGGTGG---AGAAAGAACAATTCCGATCAAT-CCAGTACAAACCCATGTCGTTAATCAA 116
|||| || ||||||| |||||||||| ||| ||||| ||||||||| ||||| |||||
Sbjct 285 ACGGCGGCGGAGAAAGATCAATTCCGAT-AATTCCAGTTCAAACCCATATCGTTGATCAA 343
Query 117 GTCCCTGAGCATTGTAAACCTCAAATCCTAACACC 151
||||| || ||| ||||||||||||||| | ||||
Sbjct 344 GTCCCCGATCATCGTAAACCTCAAATCCCATCACC 378
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 16236482927
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5