BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg939971
Length=1822
Score E
Sequences producing significant alignments: (Bits) Value
AT5G05450.1 | Symbols: | P-loop containing nucleoside triphosp... 3049 0.0
AT1G71370.1 | Symbols: | DEA(D/H)-box RNA helicase family prot... 957 0.0
AT1G71280.1 | Symbols: | DEA(D/H)-box RNA helicase family prot... 451 1e-125
> AT5G05450.1 | Symbols: | P-loop containing nucleoside triphosphate
hydrolases superfamily protein | chr5:1612006-1615356
FORWARD LENGTH=2014
Length=2014
Score = 3049 bits (1651), Expect = 0.0
Identities = 1768/1825 (97%), Gaps = 5/1825 (0%)
Strand=Plus/Plus
Query 1 GAGAGGCAGCGGCGGCCGTCATGGATTCATCGGCGAACATAAACAAAGCTTTAACGGAGA 60
|||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
Sbjct 52 GAGAGGCAGCGGCGGCCGTCATGGATTCATCGGCGAACATAAACAAAGCTCTAACGGAGA 111
Query 61 CGCGTTTCTCCGACCTCGAACCTCCACTCTCCGGAGATATCATTGAGGCGCTTAATCAGT 120
||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
Sbjct 112 CGCGTTTCTCCGACCTCGAACCTCCACTCTCCGGAGATATCATTGAGGCTCTTAATCAGT 171
Query 121 CTGATTTCGAGTTCTGCACTCCGGTTCAAGCCGCGACGATTCCTTTACTCTGTAGTTACA 180
|||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
Sbjct 172 CTGATTTCGAGTTCTGCACTCCGGTTCAAGCCGCGACGATCCCTTTACTCTGTAGTTACA 231
Query 181 AGGATGTTGCCGTCGACGCTGCCACCGGTTCTGGAAAAACCCTAGCTTTCGTCG-TCCCT 239
||||||||||||||||||||||||| |||||||||||||||||||||||||||| ||||
Sbjct 232 AGGATGTTGCCGTCGACGCTGCCACTGGTTCTGGAAAAACCCTAGCTTTCGTCGTTCCC- 290
Query 240 CTCGTTGAAATCCTCCGTCGATCCACCTCATTCCCTCCGAAGCCTCATCAGGTCATGGGG 299
||||| ||||||||||||||||||||||||||||||||||||||||| ||||||||||||
Sbjct 291 CTCGTAGAAATCCTCCGTCGATCCACCTCATTCCCTCCGAAGCCTCACCAGGTCATGGGG 350
Query 300 GTGATTATATCGCCAACAAGAGAGCTATCGACGCAGATATATAATGTGGCACAACCATTT 359
||||||||||||||||| ||||||||||| ||||||||||||||||| ||||||||||||
Sbjct 351 GTGATTATATCGCCAACGAGAGAGCTATCAACGCAGATATATAATGTCGCACAACCATTT 410
Query 360 GTTTCGACTTTGGCAAATGTGAACTCTGTGTTACTTGTTGGAGGCCGAGAGGTGAAAGCT 419
|||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
Sbjct 411 GTTTCGACTTTGGCAAATGTGAACTCTGTGTTGCTTGTTGGAGGCCGAGAGGTGAAAGCT 470
Query 420 GACACGAAGATTATTGAAGAAGAAGGATGCAATCTCTTGATTGGTACACCTGGAAGGCTT 479
|||| |||||||||||||||||||||||||||| |||||||||||||||||||||| |||
Sbjct 471 GACATGAAGATTATTGAAGAAGAAGGATGCAATGTCTTGATTGGTACACCTGGAAGACTT 530
Query 480 TCAGATATAATGGAACGAATGGAGATTCTCGATTTTCGAAATCTTGAGATTCTTA-TCTT 538
|| ||||| |||||||||||||||||||| ||||||||||||||||||||||||| || |
Sbjct 531 TCTGATATTATGGAACGAATGGAGATTCTGGATTTTCGAAATCTTGAGATTCTTATTC-T 589
Query 539 AGACGAGGCTGATAGGCTTTTGGAGATGGGGTTCCAAAGGCAGGTGAATTACATTATCTC 598
||| ||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 590 AGATGAGGCAGATAGGCTTTTGGAGATGGGGTTCCAAAGGCAGGTGAATTACATTATCTC 649
Query 599 ACGCTTGCCAAAGCAGAGGAGGACTGGTCTCTTTTCAGCTACACAAACAGAGGGCGTTGA 658
||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||
Sbjct 650 ACGCTTGCCAAAGCAGAGGAGGACTGGCCTCTTTTCAGCTACACAAACAGAGGGCGTTGA 709
Query 659 AGAGCTCGCCAAGGCTGGACTTAGGAACCCTGTGAGAGTTGAAGTTCGAGCTGAGGCGAA 718
|||||||||||||||||| ||||||||||||||||||||||||||||||||| || ||||
Sbjct 710 AGAGCTCGCCAAGGCTGGGCTTAGGAACCCTGTGAGAGTTGAAGTTCGAGCTAAGTCGAA 769
Query 719 GTCGGAATCTTCCCAGCAATTTACAAACTCGAAAACCCCTTCTGGTCTTCATCTTGAGTA 778
||||||||| ||||||||||| ||||||||||||||||||||||||||||||||||||||
Sbjct 770 GTCGGAATCATCCCAGCAATTGACAAACTCGAAAACCCCTTCTGGTCTTCATCTTGAGTA 829
Query 779 TATCGAATGCGAAGCAGATAAGAAATCATCACAACTAGTGGATCTCCTCATCAAGAACAG 838
||| ||||||||||||||||| ||||||||||||||||| ||||||||||||||||||||
Sbjct 830 TATGGAATGCGAAGCAGATAAAAAATCATCACAACTAGTTGATCTCCTCATCAAGAACAG 889
Query 839 TGATAAAAAGCTTATAGTATTCTTCATGACCTGTGCTTCTGTTGACTACTGGGGACTCGT 898
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 890 TGATAAAAAGCTTATAGTATTCTTCATGACCTGTGCTTCTGTTGACTACTGGGGACTCGT 949
Query 899 ACTTTCAAAAATTCCTGCCCTGAAGTCTATATCTTTAATTCCTATCCATGGGGATATGAA 958
||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||
Sbjct 950 ACTTTCAAAAATTCCTGCCCTGAAGTCCATATCTTTAATTCCTATCCATGGGGATATGAA 1009
Query 959 GCAGAATGCAAGAGACAAGGCATTAGCTTCGTTTACTAAAGCATCAAGCGGTGCCCTTCT 1018
|||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1010 GCAGAATGCAAGAGACAAGGCATTAGCTTCGTTTACTAAAGCATCAAGTGGTGCCCTTCT 1069
Query 1019 GTGCACAGACGTTGCTGCACGTGGACTTGATATTCCAGGCATTGATTATGTAGTTCAGTA 1078
||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
Sbjct 1070 GTGCACAGACGTTGCTGCACGTGGACTTGATATTCCAGGCATTGATTATGTTGTTCAGTA 1129
Query 1079 TGATCCCCCACAAGACCCAAATATGTTCAACCACAGGGCCGGCAGAACTGCAAGATTGGG 1138
||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||
Sbjct 1130 TGATCCCCCACAAGACCCAAATATGTTCAACCACAGGGCCGGCAGAACTGCAAGATTAGG 1189
Query 1139 AAGACTAGGGAAGGCCATTGTGTTTTTACTGCCTAAGGAGGAAGCCTATGTAGAGTTTAT 1198
||||| ||||| ||||||||||||||||||||| ||||| ||||||||||||||||||||
Sbjct 1190 AAGACAAGGGAGGGCCATTGTGTTTTTACTGCCCAAGGAAGAAGCCTATGTAGAGTTTAT 1249
Query 1199 GCGCATAAGAAGAGTCCCTCTGGAAGAGAGAAAATGCTCTGAGGACGCATCCGATGTCAT 1258
||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 1250 GCGCATAAGAAGAGTCCCTTTGGAAGAGAGAAAATGCTCTGAGGACGCATCCGATGTCAT 1309
Query 1259 CCCGATCATACGTTCAGCTGCCATGAAGGACCGAGCAGTGTTGGAGAAGGGATTAAAGGC 1318
|||||| ||||||||||||||||||||||||||||||||| |||||||||||||||||||
Sbjct 1310 CCCGATTATACGTTCAGCTGCCATGAAGGACCGAGCAGTGATGGAGAAGGGATTAAAGGC 1369
Query 1319 ATTTGTTTCTTTTGTCCGTGCTTATAAGGAGCATCACTGCTCTTTCATTTTCAGATGGAA 1378
||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1370 ATTTGTTTCCTTTGTCCGTGCTTATAAGGAGCATCACTGCTCTTTCATTTTCAGATGGAA 1429
Query 1379 AGAACTTGAAATTGGAGAGTTAGCCATGGGATATGGCCTCTTGTATCTTCCTTCAATGTC 1438
||| ||||| |||||| |||||||||||||||||||||||||||||||||||||||||||
Sbjct 1430 AGACCTTGAGATTGGAAAGTTAGCCATGGGATATGGCCTCTTGTATCTTCCTTCAATGTC 1489
Query 1439 TGAAGTTAAACAACACAGACTTTCCAGTGAGGGTTTCACACCTGTTGAAGGTGTCAAGTT 1498
|||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
Sbjct 1490 TGAAGTTAAACAACACAGACTTTCCAGTGAGGGTTTCACACCAGTTGAAGGTGTCAAGTT 1549
Query 1499 TGACGAAATAAAATTCAAGGACAAATCTCGGgagaaacaaagacagcaaaacttacaagt 1558
||| |||||||||||||||||||||| |||||||||||||||||||||||||||||||||
Sbjct 1550 TGAAGAAATAAAATTCAAGGACAAATATCGGGAGAAACAAAGACAGCAAAACTTACAAGT 1609
Query 1559 aaggaaagagaaacggcaagaagagaaaagagagaaaggcaagagaaaaagGGTTGATGC 1618
||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
Sbjct 1610 AAGGAAAGAGAAACGGCAAGAAGAGAAAAAAGAGAAAGGCAAGAGAAAAAGGGTTGATGC 1669
Query 1619 TCCTACTACTAATGATCCTAAGAAAGCGTCGAGAAAGTTAACAGGGAAGCAAAGACAAAC 1678
| || ||| ||||||||||||||||||||||||||||||||| ||||| |||||||||||
Sbjct 1670 TTCTGCTAGTAATGATCCTAAGAAAGCGTCGAGAAAGTTAACGGGGAAACAAAGACAAAC 1729
Query 1679 TATTCAAACTGCTGAAGATGAAGAAGTGATGGATCGAGATTATAAGTTAATGATAAAAGT 1738
|||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1730 TATTCAAACTGCTGAAGATGAAGAAGTGATGGATCGAGATTATAAGTTGATGATAAAAGT 1789
Query 1739 AAAGAAAGGGTTAATTAAAGAAGATGAATATGAAAGACTTACAGGAGATGATGATTTATT 1798
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1790 AAAGAAAGGGTTAATTAAAGAAGATGAATATGAAAGACTTACAGGAGATGATGATTTATT 1849
Query 1799 CTGATGAGAAGAAACAAA-CCTGCA 1822
|||||||||||||||||| ||||||
Sbjct 1850 CTGATGAGAAGAAACAAAACCTGCA 1874
> AT1G71370.1 | Symbols: | DEA(D/H)-box RNA helicase family protein
| chr1:26897005-26899434 REVERSE LENGTH=1960
Length=1960
Score = 957 bits (518), Expect = 0.0
Identities = 912/1097 (83%), Gaps = 47/1097 (4%)
Strand=Plus/Plus
Query 742 CAAACTCGAAAACCCCTTCTGGTCT-TCATCT-TGAGTATATCGAATGCGAAGCAGAT-A 798
|||| |||||||| |||||||||| | || | ||||||| | |||||||||||||| |
Sbjct 748 CAAAGTCGAAAACGTCTTCTGGTCTGT-AT-TGTGAGTATTTAAAATGCGAAGCAGATCA 805
Query 799 AGAAATCATCACAACTAGTGGATCTCCTCATCAAGAACAGTGATAAAAAGCTTATAGTAT 858
| || ||||||||||||||| ||||||| ||| |||||| ||||||||||| | ||||
Sbjct 806 A-AAGTCATCACAACTAGTGCATCTCCTGATCGAGAACAAAAATAAAAAGCTTGTCGTAT 864
Query 859 TCTTCATGACCTGTGCTTCTGTTGACTACTGGGGACTCGTACTTTCAAAAATTCCTGCCC 918
|||||||||| ||||||| |||||| |||||||||||||||||||||||||||||| |||
Sbjct 865 TCTTCATGACATGTGCTTGTGTTGATTACTGGGGACTCGTACTTTCAAAAATTCCTACCC 924
Query 919 TGAAGTCTATAT-CTT-TAATTCCTATCCATGGGGATATGAAGCAGAATGCAAGAGACAA 976
| ||||| || | ||| | |||| | ||||||| | ||| | ||||| | |||||||
Sbjct 925 TCAAGTCCATTTCCTTCT-TTTCC-ACCCATGGGAAGATGGATCAGAAAGGTAGAGACAC 982
Query 977 GGCATTAGCTTCGTTTACTA-AAGCATCAAGCGGTGCCCTTCTGTGCACAGACGTTGCTG 1035
|||||||||||||||||| | | ||||||||||||| |||||| |||||||| || ||||
Sbjct 983 GGCATTAGCTTCGTTTAC-AGAGGCATCAAGCGGTGTCCTTCTATGCACAGATGTCGCTG 1041
Query 1036 CACGTGGACTTGATATTCCAGGCATTGATTATGTAGTTCAGTATGATCCCCCACAAGACC 1095
||||||||||||| ||||||||||| ||||| || |||||||||||||||||||||||||
Sbjct 1042 CACGTGGACTTGACATTCCAGGCATCGATTACGTTGTTCAGTATGATCCCCCACAAGACC 1101
Query 1096 CAAATATGTTCAACCACAGGGCCGGCAGAACTGCAAGATTGGGAAGACTAGGGAAGGCCA 1155
|| || | || | ||| |||| ||||| ||||||||| ||| ||||| ||| | |||||
Sbjct 1102 CAGATGTATTTATCCATAGGGTTGGCAGGACTGCAAGAATGGAAAGACAAGGTAGGGCCA 1161
Query 1156 TTGTGTTTTTACTGCCTAAGGAGGA-AGCCTATGTAGAGTTTATGCGCATAAGAAGAGTC 1214
||||||||||| |||| ||||| || || |||||||||||| |||||||| |||| |||
Sbjct 1162 TTGTGTTTTTAATGCCCAAGGA-GACAGACTATGTAGAGTTCATGCGCATTCGAAGGGTC 1220
Query 1215 CCTCTGGAAGAGAGAAAATGCTCTGAGGACGCATCCGATGTCATCCCGATCATACGTTCA 1274
||||| | |||||||||||||||||| |||| || |||||||||||||| |||||||||
Sbjct 1221 CCTCTTCAGGAGAGAAAATGCTCTGAGAACGCTTCTGATGTCATCCCGATTATACGTTCA 1280
Query 1275 GCT-GCCATGAAGGACCGAGCAGTGTTGGAGAAGGGATTAAAGGCATTTGTTTCTTTTGT 1333
|| ||||| |||||||| ||||||||||||||||||||| ||||||||||||| |||||
Sbjct 1281 -CTAGCCATCAAGGACCGGGCAGTGTTGGAGAAGGGATTACAGGCATTTGTTTCCTTTGT 1339
Query 1334 CCGTGCTTATAAGGAGCATCACTGCTCTTTCATTTTCAGATGGAAAGAACTTGAAATTGG 1393
||||||||||||||||||||||||||||| ||||||||| ||||||| ||||||||| ||
Sbjct 1340 CCGTGCTTATAAGGAGCATCACTGCTCTTACATTTTCAGTTGGAAAGGACTTGAAATCGG 1399
Query 1394 AGAGTTAGCCATGGGATATGGCCTCTTGTATCTTCCTTCA-ATGTCTGAAGTTAAACAAC 1452
| |||||||||||||||||||| |||||| | |||||| | || |||||||||||||||
Sbjct 1400 AAAGTTAGCCATGGGATATGGCATCTTGTCTTTTCCTT-ACATATCTGAAGTTAAACAAG 1458
Query 1453 ACAGACTTTCCAGTGAGGGTTTCACACCTGTTGAAGGTGTCAAGTTTGACGAAATAAAAT 1512
||||| || || || ||||| |||||||| |||| ||| |||||| || |||||||
Sbjct 1459 ACAGAATTGGCATTGTCGGTTTTACACCTGTCCAAGGCATCACGTTTGAGGACATAAAAT 1518
Query 1513 TCAAGGACAAATCTCGGgagaaacaaagacagcaaaacttacaagtaaggaaagagaaac 1572
|||| |||||||||||||||||||||||||||||||||||| || ||||||||| ||||
Sbjct 1519 ACAAGAACAAATCTCGGGAGAAACAAAGACAGCAAAACTTACTAGCAAGGAAAGACAAAC 1578
Query 1573 ggcaagaagagaaa-agagagaaaggcaagagaaaaagGGTTGAT-GCTCCTACTACTAA 1630
|||| |||| ||| |||| |||| |||||| ||| | | | | | | | |
Sbjct 1579 TGCAACAAGA-AAAGAGAGGGAAACGCAAGA-AAA--GC-TCCAAAGAGGCGG-T--TGA 1630
Query 1631 TGATCCTAAGAAAGCGTCGAGAAAG------TTAACAGGGAAGCAAAGACAAACTATTCA 1684
|||| |||| ||||||||||||||| ||||| |||| ||||||||||||||| ||
Sbjct 1631 TGATTCTAACAAAGCGTCGAGAAAGAGAAAGTTAACGGGGAGGCAAAGACAAACTATCCA 1690
Query 1685 AACTGCTG-AAGATGAAGAAGTGATGGATC-GAGATTATAAG-TTAATG-ATAAAAGTAA 1740
||| || | |||||||||||| ||| || ||||||||| | ||| || | |||| | |
Sbjct 1691 AACAGC-GCAAGATGAAGAAGAAATGAATTTGAGATTATA-GCTTACTGGA-AAAACTGA 1747
Query 1741 AGAAAGGGTTAATTAAAGAAGATGAATATGA-AAGACTTACAGGAGATGATGATTTAT-T 1798
||||||| | ||||| ||||||||| | || || ||||||||||| | ||||||| | |
Sbjct 1748 AGAAAGGCTCAATTATAGAAGATGATTTTGCTAA-ACTTACAGGAGCTCATGATTT-TGT 1805
Query 1799 CTGATGAGAAGAAACAA 1815
||||| | ||||| |||
Sbjct 1806 CTGAT-A-AAGAAGCAA 1820
> AT1G71280.1 | Symbols: | DEA(D/H)-box RNA helicase family protein
| chr1:26870141-26872152 REVERSE LENGTH=1519
Length=1519
Score = 451 bits (244), Expect = 1e-125
Identities = 464/566 (82%), Gaps = 31/566 (5%)
Strand=Plus/Plus
Query 1265 CATACGTTCAGCT-GCCATGAAGGACCGAGCAGTGTTGGAGAAGGGATTAAAGGCATTTG 1323
|||||||||| || ||||| |||||||| | |||||||||||||||||||||||||||||
Sbjct 939 CATACGTTCA-CTAGCCATCAAGGACCGGGAAGTGTTGGAGAAGGGATTAAAGGCATTTG 997
Query 1324 TTTCTTTTGTCCGTGCTTATAAGGAGCATCACTGCTCTTTCATTTTCAGATGGAAAGAAC 1383
|||| |||||||||||||||||||||||||| ||||||| ||||||||| ||||||| ||
Sbjct 998 TTTCCTTTGTCCGTGCTTATAAGGAGCATCAATGCTCTTACATTTTCAGTTGGAAAGGAC 1057
Query 1384 TTGAAATTGGAGAGTTAGCCATGGGATATGGCCTCTTGTATCTTCCTT-CAATGTCTGAA 1442
||||||| ||| |||||||||||||||||||| |||||| | |||||| || | ||||||
Sbjct 1058 TTGAAATCGGAAAGTTAGCCATGGGATATGGCATCTTGTCTTTTCCTTACA-TATCTGAA 1116
Query 1443 GTTAAACAACACAGACTTTCCAGTGAGGGTTTCACACCTGTTGAAGGTGTCAAGTTTGAC 1502
||||||||| ||||| || || || ||||| |||||||| |||| ||| ||||||
Sbjct 1117 GTTAAACAAGACAGAATTGGCATTGTCGGTTTTACACCTGTCCAAGGCATCACGTTTGAG 1176
Query 1503 GAAATAAAATTCAAGGACAAATCTCGGgagaaacaaagacagcaaaacttacaagtaagg 1562
|| |||||||||||| |||||||||||||||||||||| |||||||||||| || ||||
Sbjct 1177 GACATAAAATTCAAGAACAAATCTCGGGAGAAACAAAGGCAGCAAAACTTATTAGCAAGG 1236
Query 1563 aaagagaaacggcaagaagagaaa-agagagaaaggcaagagaaaaagGGTTGAT-GCTC 1620
||||| |||| |||| |||| ||| |||| |||| |||||| ||| | | | |
Sbjct 1237 AAAGACAAACTGCAACAAGA-AAAGAGAGGGAAACGCAAGA-AAA--GC-TCCAAAGAGG 1291
Query 1621 CTACTACTAATGATCCTAAGAAAGCGTCGAGAAAG------TTAACAGGGAAGCAAAGAC 1674
| | | ||||| |||| ||||||||||||||| ||||| |||| ||||||||
Sbjct 1292 CGG-T--TGATGATTCTAACAAAGCGTCGAGAAAGAGAAAGTTAACGGGGAGGCAAAGAC 1348
Query 1675 AAACTATTCAAACTGCTGAAGATGAAGAAGTGATGGATC-GAGATTATAAG-TTAATGAT 1732
||||||| ||||| ||| |||||||||||| ||| || ||||||||| | ||| |||
Sbjct 1349 AAACTATCCAAACAGCTCAAGATGAAGAAGAAATGAATTTGAGATTATA-GCTTACTGAA 1407
Query 1733 AAAAGTAAAGAAAGGGTT-AATTAAAGAAGATGAATATGA-AAGACTTACAGGAGATGAT 1790
|||| | |||||||| || ||||| ||||||||| | || || ||||||||||| | ||
Sbjct 1408 AAAACTGAAGAAAGG-TTCAATTACAGAAGATGATTTTGCTAA-ACTTACAGGAGCTCAT 1465
Query 1791 GATTTAT-TCTGATGAGAAGAAACAA 1815
||||| | |||||| | ||||| |||
Sbjct 1466 GATTT-TGTCTGAT-A-AAGAAGCAA 1488
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 90160178820
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5