BLASTN 2.2.26+
Reference:
Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000),
"A greedy algorithm for aligning DNA sequences", J Comput Biol 2000;
7(1-2):203-14.
Database: TAIR10_cdna_20110103_representative_gene_model_updated
33,602 sequences; 51,074,197 total letters
Query= Ahg940242
Length=3625
Score E
Sequences producing significant alignments: (Bits) Value
AT5G07810.1 | Symbols: | SNF2 domain-containing protein / heli... 5616 0.0
> AT5G07810.1 | Symbols: | SNF2 domain-containing protein / helicase
domain-containing protein / HNH endonuclease domain-containing
protein | chr5:2491283-2498484 REVERSE LENGTH=3702
Length=3702
Score = 5616 bits (3041), Expect = 0.0
Identities = 3430/3616 (95%), Gaps = 34/3616 (1%)
Strand=Plus/Plus
Query 21 ATGGAGGTTTCCGAAGAGCAACGGCGGAGAGACGAAGCGAAC-CGATTAGCTTTTGCGGA 79
||||||||||||||||||||| |||| |||||||||||| || || ||||||||||||||
Sbjct 1 ATGGAGGTTTCCGAAGAGCAAAGGCGAAGAGACGAAGCG-ACTCGGTTAGCTTTTGCGGA 59
Query 80 GAAACGAAAGAC-GTCGTTTGATTCTTCAGAAAACCCACAGCGACAACAAGATTTCAGGT 138
|||||||||| | |||||| |||||||| |||||||||||||||||||||||||||||||
Sbjct 60 GAAACGAAAG-CAGTCGTTCGATTCTTCGGAAAACCCACAGCGACAACAAGATTTCAGGT 118
Query 139 TAGCTAAATGTCGAAAGCTCGACGGTGGTAAAGATGTCTGTTTTCAAGAAGGGTATCGGA 198
||||||||||||||||||| |||||| |||| ||||| ||| |||||||||||||||||
Sbjct 119 TAGCTAAATGTCGAAAGCTTGACGGTAGTAACGATGTGTGTCCTCAAGAAGGGTATCGGA 178
Query 199 ACTACGAACCGGAACGAGGATTGAGTACTAGTGTACCGGAAAAGTTCCGCGTCAGGCTCG 258
|||||||||||| ||||| ||||||||||||||| ||||||||||| |||||||||||||
Sbjct 179 ACTACGAACCGGTACGAGTATTGAGTACTAGTGTGCCGGAAAAGTTTCGCGTCAGGCTCG 238
Query 259 AGATCTGTTCTCCTGATTCGTTTTCGGTGACGCCGGTTCAATTGCAAGGGTTTCGATGCC 318
|||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 239 AGATCTGTTCTCCTGATTCGTTTTCGGTTACGCCGGTTCAATTGCAAGGGTTTCGATGCC 298
Query 319 CAGAAGAGCAAGAGTGTCTTCGTCAATTGAGAGAGATTCTATCTGAGGCGATTCCTTTAC 378
| ||||| |||||||| ||||||||||||||| ||||||||||||| |||||||||||||
Sbjct 299 CTGAAGAACAAGAGTGCCTTCGTCAATTGAGAAAGATTCTATCTGATGCGATTCCTTTAC 358
Query 379 ACTACACTCAAAATGATGACGGAGGAAAAGCTGGGGTTTATAAGATTAGGGACTACAATA 438
||||||||||||||||||| ||||||||||||||||||||||||||||| ||||||||||
Sbjct 359 ACTACACTCAAAATGATGATGGAGGAAAAGCTGGGGTTTATAAGATTAGAGACTACAATA 418
Query 439 TGGTCTCTGGATGTCTAAAAAGGTCCAAGAGCGTTGAAGTTGAAGAGATACCTTGGAAAA 498
||||||||||||||| |||||||||||||||||||||||||||| | ||||||||||||
Sbjct 419 AGGTCTCTGGATGTCTTAAAAGGTCCAAGAGCGTTGAAGTTGAAGGGGTACCTTGGAAAA 478
Query 499 CGTTGGCTGTTGTTGAGAAACTTTCCCAGTCAT-TCATTTCTGGGAAATGGCAACCTTGT 557
||||||||||||||||||||||||| ||||||| | ||||| ||||||||||||||||||
Sbjct 479 CGTTGGCTGTTGTTGAGAAACTTTCTCAGTCATAT-ATTTCCGGGAAATGGCAACCTTGT 537
Query 558 ATACCTGAACATTATACGGAGGAGAAGGTTGAACAACTGATTGAAACCCTGCCAAGAAAA 617
||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
Sbjct 538 TTACCTGAACATTATACGGAGGAGAAGGTTGAACAACTGATTGAAACCCTGCCAAGGAAA 597
Query 618 TTGGTGAATGCCCTTTTGCCTTTCCAACTTGATGGTCTAAGATTTGGTTTACGGAGAGGT 677
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 598 TTGGTGAATGCCCTTTTGCCTTTCCAACTTGATGGTCTAAGATTTGGTTTACGGAGAGGT 657
Query 678 GGCAGGTGTTTCATTGCTGATGAAATGGGGCTTGGAAAGACGCTTCAGGCCATTGCCATT 737
||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||
Sbjct 658 GGCAGGTGTTTCATTGCTGATGAAATGGGGCTTGGAAAGACGCTTCAGGCCATCGCCATT 717
Query 738 GCTGGTTGCTTCATAAGTGAGGGTTCCATACTTGTTGTCTGTCCAGCTGTTCTGCGTTTC 797
|| ||||||||||||||||||||||||||||||||||||||||| |||||| ||||||||
Sbjct 718 GCCGGTTGCTTCATAAGTGAGGGTTCCATACTTGTTGTCTGTCCTGCTGTTTTGCGTTTC 777
Query 798 TCATGGGCAGAAGAATTGGAACGTTGGTTGCCATTTTGTTTGCCTTCTGACATACATCTC 857
||||||||||||||||||||||||||||||||| |||||||||||||||| ||||||||
Sbjct 778 ACATGGGCAGAAGAATTGGAACGTTGGTTGCCATCTTGTTTGCCTTCTGACGTACATCTC 837
Query 858 GTTTTCGGTCATCAAGATAATCCTGCCTATTTACCAAGATGGCCAAAAGTTGTGGTTATC 917
|| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 838 GTATTCGGTCATCAAGATAATCCTGCCTATTTACCAAGATGGCCAAAAGTTGTGGTTATC 897
Query 918 TCATACAAGATGCTTCAGCATCTCCGGACGACCATGCTAGAGCGAGAATGGGCCCTCTTG 977
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 898 TCATACAAGATGCTTCAGCATCTCCGGACGACCATGCTAGAGCGAGAATGGGCCCTCTTG 957
Query 978 ATAGTAGATGAATCTCATCATCTACGGTGTTCGaaaaaaaaGTCAGATCCACCTGAGATA 1037
|||||||||||||||||||||||||| ||||||||||| |||||||||||||||||||||
Sbjct 958 ATAGTAGATGAATCTCATCATCTACGATGTTCGAAAAAGAAGTCAGATCCACCTGAGATA 1017
Query 1038 AAGACAGTCCTTAATGTTGCTGAAAAGGTTAAGCACATAGTTCTGTTGTCAGGAACTCCT 1097
|||||||||||| |||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 1018 AAGACAGTCCTTGATGTTGCTGAAAAGGTTAAGCACATAATTCTGTTGTCAGGAACTCCT 1077
Query 1098 TCTATATCGAGGCCCTTCGACATTTTCCATCAGATAAACATCCTATGGCCTGGTTTGCTG 1157
||| ||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
Sbjct 1078 TCTGTATCGAGGCCCTTCGACATTTTCCATCAGATAAACATGCTATGGCCTGGTTTGCTG 1137
Query 1158 GGAAAAGACAAGTATGAGTTTGCGAAAACCTATTGTGAAGTTGGGCTTGTCAGAGGTATG 1217
|||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1138 GGAAAAGATAAGTATGAGTTTGCGAAAACCTATTGTGAAGTTGGGCTTGTCAGAGGTATC 1197
Query 1218 CAAGGGAAGATTTTCCAGGATTTTTCAAAGGGTACTCGCTTACTGGAGTTAAACATACTG 1277
|||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||
Sbjct 1198 CAAGGGAAGATTTTCCAGGATTTTTCCAAGGGTACTCGCTTACTGGAGTTAAACATACTG 1257
Query 1278 CTAAACCAAACAGTGATGATAAGAAGACTGAAACAGCATGTGTTAACACAGCTACCCCCA 1337
|||||||||||||||||||||||||||||||| |||||| ||||||||||| ||||||||
Sbjct 1258 CTAAACCAAACAGTGATGATAAGAAGACTGAAGCAGCATTTGTTAACACAGTTACCCCCA 1317
Query 1338 AAACGTAGGCAAATCGTAACCATCTTGCTAAAGAAGTCTGATATATCTTTAGCAATGTCT 1397
||||||||||||||||||||||||||||||||||||||||||||| ||||||||||| ||
Sbjct 1318 AAACGTAGGCAAATCGTAACCATCTTGCTAAAGAAGTCTGATATAGCTTTAGCAATGGCT 1377
Query 1398 ATTGTCAGTGAGGCTAATCAGAAGGCTAATAAACAAAAGGATGGTGCTATAGCAGAAGTC 1457
|||||| | | | | ||||||| ||||||||||||||||||||||||||||||
Sbjct 1378 ATTGTC----A-G-----C-G-AGGCTAAGAAACAAAAGGATGGTGCTATAGCAGAAGTC 1425
Query 1458 ACAGAGAACTCACATGAACCTAAAGATCAAAATGCTCAAGGATCAAATGAAGCTGGACAT 1517
|||||||| |||||||||||| || |||||||||| |||||||||||||||||||||
Sbjct 1426 ACAGAGAAGTCACATGAACCT---GACCAAAATGCTCGTGGATCAAATGAAGCTGGACAT 1482
Query 1518 GTAAATGCAGAGAATTCTGATGGTCCGAACTCTGACAGAGAGAATCAACTATGTGGGAAA 1577
||||| ||||||||||||||||| || |||||||||| ||||||||||||||||||||||
Sbjct 1483 GTAAACGCAGAGAATTCTGATGGCCCTAACTCTGACAAAGAGAATCAACTATGTGGGAAA 1542
Query 1578 CTTTCCTATCAACAACTTGGCATTGCAAAGCTTTCTGCCTTTCGTGAATGGTTATCCCTA 1637
|||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
Sbjct 1543 CTTTCCTATCAACAACTTGGCATTGCAAAGCTTTCTGCCTTTCGTGAATGGTTATCTCTA 1602
Query 1638 CATCCACTTCTATCAGGATTGGATTATACCCCAGAAGATATTGATGGAGACACG-AGCTC 1696
|||||||||||||||||||||||||||||||||||||| ||||||||||||| | |||||
Sbjct 1603 CATCCACTTCTATCAGGATTGGATTATACCCCAGAAGAAATTGATGGAGACA-GAAGCTC 1661
Query 1697 AACTAAAATGGTCGTTTTTGCTCATCACCATAAGGTTCTGGATGGAATTCAGGAATTTAT 1756
||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
Sbjct 1662 AACTAAAATGGTCGTTTTTGCACATCACCATAAGGTTCTGGATGGAATTCAGGAATTTAT 1721
Query 1757 ATGTGATAAAGGGATTGGTTTTGTTCGCATTGATGGGACGACGTTGCCAAGAGATAGACA 1816
|||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
Sbjct 1722 ATGTGATAAAGGGATTGGTTTTGTTCGCATTGATGGGACGACATTGCCAAGAGATAGACA 1781
Query 1817 ATTGGCTGTACAGTCATTTCAGTTTTCTAGTGAGGTAAAAGTTGCAATAATTGGCGTAGA 1876
|||||||||||||||||||||||||||||||||||||||| ||||||||||||| |||||
Sbjct 1782 ATTGGCTGTACAGTCATTTCAGTTTTCTAGTGAGGTAAAAATTGCAATAATTGGTGTAGA 1841
Query 1877 AGCTGGAGGAGTTGGACTGGATTTTTCAGCAGCACAAAATGTTGTGTTCCTTGAATTACC 1936
||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||
Sbjct 1842 AGCTGGAGGAGTTGGACTGGATTTTTCAGCAGCACAAAATGTTGTGTTCCTTGAATTGCC 1901
Query 1937 TAAAACACCGTCACTGTTGCTTCAGGCTGAGGATAGAGCACATAGGCGAGGCCAAACAAG 1996
|||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1902 TAAAACACCGTCTCTGTTGCTTCAGGCTGAGGATAGAGCACATAGGCGAGGCCAAACAAG 1961
Query 1997 TGCAGTCAATGTATACATCTTCTGCGCAAAGGATACTATGGACGAGTCAAATTGGCAAAA 2056
|||||| |||||||||||||||||||| ||||||||||||||||||||||||||||||||
Sbjct 1962 TGCAGTAAATGTATACATCTTCTGCGCGAAGGATACTATGGACGAGTCAAATTGGCAAAA 2021
Query 2057 CCTGAACAAGAAGTTGCACCGTATTTCATCTACCACTGATGGAAAATATGATGGGAAGAC 2116
|| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2022 TCTAAACAAGAAGTTGCACCGTATTTCATCTACCACTGATGGAAAATATGATGGGAAGAC 2081
Query 2117 CGAAATCGAAATTGAAAGAGCATATATTTTCAAGCCTGCTGAAGAAAGCAGTGAAAGAGA 2176
||||||||||||| | |||||| ||||||||||||||||||||||||||||||||||||
Sbjct 2082 TGAAATCGAAATTGGAGGAGCATCTATTTTCAAGCCTGCTGAAGAAAGCAGTGAAAGAGA 2141
Query 2177 AGTTCTTGAAGCACAACCATCAAAAGCTAATACTGTAGTAGCTGATAAGATTGTTGAAAG 2236
||||||||||| ||||||||| || | ||||||||||||||||||||||||||||||||
Sbjct 2142 AGTTCTTGAAGGGCAACCATCAGAATCAAATACTGTAGTAGCTGATAAGATTGTTGAAAG 2201
Query 2237 CTGTGACGATCTGGGATCTGAGACAGATGTATCAAATAGTATTGATCTCAAGGACGACAT 2296
||||||||||| |||| ||||||||||||| ||||||| |||||||||||||||||||||
Sbjct 2202 CTGTGACGATCCGGGAACTGAGACAGATGTGTCAAATACTATTGATCTCAAGGACGACAT 2261
Query 2297 GACTTCTCA-TCTGGAAATATTAGAAGTCTGTAAATTTGTTGAGAATGGTTCCGGCTCTG 2355
||||||||| | |||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 2262 GACTTCTCAAT-TGGAAATATTAGAAGTCTGTCCTTTTGTTGAGAATGGTTCCGGCTCTG 2320
Query 2356 AG-ATGAGATCTTCTGGCACCATTTGTTTAACTATGTTAGACCAGGAAAACCAAGAAAAT 2414
| ||||| |||||||||||||||| ||| |||||||||| |||||||||||||||||||
Sbjct 2321 -GCATGAGGTCTTCTGGCACCATTTCTTTGACTATGTTAGCCCAGGAAAACCAAGAAAAT 2379
Query 2415 CATCAGCCTAAAAATCTTATTGCTGATGACGGGCTTGTGAAAGAATTTGATTCCAGTTCC 2474
||| ||||||||||||||||||||||||| ||||||||||||||| ||||||||||||||
Sbjct 2380 CATAAGCCTAAAAATCTTATTGCTGATGATGGGCTTGTGAAAGAAGTTGATTCCAGTTCC 2439
Query 2475 ATTTTTCCACTCATAGATTCTCTCAGATTTGAGGTGAGCCAAAACACTGGAAGAATTCAT 2534
||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
Sbjct 2440 ATTTTTCCACTCATAGATTCTCTGAGATTTGAGGTGAGCCAAAACACTGGAAGAATTCAT 2499
Query 2535 TTATACTCTTGCATTCTTGGGAAAGATCCACGACCAAGGCCTCATTTCCAGAACTTCCGC 2594
|||||||||||||||| ||||||||||||| ||||||||||||||||||| ||||||||
Sbjct 2500 TTATACTCTTGCATTCCTGGGAAAGATCCAAGACCAAGGCCTCATTTCCAAAACTTCCGT 2559
Query 2595 CCAGAGGAGATTGAAGCAAGCAATACAAGTCAGGGTCCCAATAAAGaaaaaaaTCCTGAA 2654
|||||||||||||||||||||||| ||||||||||| ||| ||||||||||||||||||
Sbjct 2560 CCAGAGGAGATTGAAGCAAGCAATCCAAGTCAGGGTACCACGAAAGAAAAAAATCCTGAA 2619
Query 2655 TCCATTACAGATGATCCGGTTCATGTACTTGCTATCTTAGAATTTATGAAAGAGTGGAAA 2714
|||||||| ||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2620 TCCATTACGGATGACCCGGTTCATGTACTTGCTATCTTAGAATTTATGAAAGAGTGGAAA 2679
Query 2715 TCACTACGGCCAATCGAAAAGCGGAAACTACTTGGAAAGCCTTTGCAACTACCTTTATCA 2774
|| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2680 TCGCTACGGCCAATCGAAAAGCGGAAACTACTTGGAAAGCCTTTGCAACTACCTTTATCA 2739
Query 2775 CTGGAGTTGAGCTACTTGAGTGAAAGTACTAGTCACAACAACGAGGGATTGCTTAGGGGA 2834
||||||||||||||| |||||||||||||||||||||||| |||||||||||||||||||
Sbjct 2740 CTGGAGTTGAGCTACCTGAGTGAAAGTACTAGTCACAACAGCGAGGGATTGCTTAGGGGA 2799
Query 2835 GGGAGCAAAAGACGAAACACACCCTTTTCTGAGATCAGTATTCCTGTACCTGAAAATGCA 2894
||||||||||||||||||||||| |||||||||||||| |||||| ||||||||||||||
Sbjct 2800 GGGAGCAAAAGACGAAACACACCGTTTTCTGAGATCAGCATTCCTTTACCTGAAAATGCA 2859
Query 2895 GTGTGGAAAAAGGTCAACCTTCGAAGTGGCCACCAAAGAAAGGAAAAAGAATACACCCAA 2954
||||||| ||||||||||||||||||||| ||||||||||||||||||||||||||||||
Sbjct 2860 GTGTGGATAAAGGTCAACCTTCGAAGTGGTCACCAAAGAAAGGAAAAAGAATACACCCAA 2919
Query 2955 GCTTGGAGTATGAGCAACGAACCACTCTGTAAACTCTGTCAAAAGCCCTGCAAGGGAAAC 3014
||||||||||||||||||||||||||||||||||||||||||||||||||| |||| ||
Sbjct 2920 GCTTGGAGTATGAGCAACGAACCACTCTGTAAACTCTGTCAAAAGCCCTGCGCGGGATAC 2979
Query 3015 AACGCCAAAGAACCTGAGTATTTTGAGGATCTTTTCTGTGATCTTGCATGCTATGAAGAT 3074
||||||||||| ||||||||||| ||||||||||||||||| |||||||||||||||||
Sbjct 2980 AACGCCAAAGAGCCTGAGTATTTAGAGGATCTTTTCTGTGAGCTTGCATGCTATGAAGAC 3039
Query 3075 TACCGGACGAGAACAAGCAGCAGATACATTCGCCAGATAGAACATGGAATTTGCACAAAC 3134
|||||||| |||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3040 TACCGGACAAGAACAAGCAGCAGATACATTCGCCAGATAGAACATGGAATTTGCACAAAC 3099
Query 3135 TGCGAATTAGACTGCCACCAACTTGTCAGACGGTTAAGGCCTTTACCATTGGAAAAACGC 3194
||| |||||||||||||||||||||||| ||||||||||||||||||| ||||||||||
Sbjct 3100 TGCAAATTAGACTGCCACCAACTTGTCAAACGGTTAAGGCCTTTACCACTGGAAAAACGA 3159
Query 3195 CGTACATACATAAACAAAGTTGCTCCAGAATTGTTTGCGCGGAAGAACCTGCTCGAAACC 3254
||||||||||||||||||| |||||||||||||||||| ||||||||| |||| ||||||
Sbjct 3160 CGTACATACATAAACAAAGCTGCTCCAGAATTGTTTGCCCGGAAGAACTTGCTTGAAACC 3219
Query 3255 CTGGTTAATGATCCAACTGAGGGTAATGCTTGGCATGCGGATCATATCATTCCAGTCTAC 3314
||||||||||||||||| ||||| ||||| ||||||||||||||||||||||||||||||
Sbjct 3220 CTGGTTAATGATCCAACGGAGGGAAATGCATGGCATGCGGATCATATCATTCCAGTCTAC 3279
Query 3315 CAAGGCGGAGGTGAATGCCGGCTAGAGAACATGAGGACACTTTGTGTGGCTTGTCATGCC 3374
||||||||||| || |||||||||||||||||||||||||| ||||||||||| ||||||
Sbjct 3280 CAAGGCGGAGGCGAGTGCCGGCTAGAGAACATGAGGACACTATGTGTGGCTTGCCATGCC 3339
Query 3375 AATGTCACTGCAGCTCAATGCGGAGAACGTAAACTGATACGGTCCAAAGCCAGGAAGCAA 3434
||||||||||||||||||| | |||||||||||||||||||||||||||||||||||||
Sbjct 3340 GATGTCACTGCAGCTCAATGTGCAGAACGTAAACTGATACGGTCCAAAGCCAGGAAGCAA 3399
Query 3435 CTCAAAAACACTTTAAACGAACTCAGAAACAATCCAAAGCAAACAGAACTGC-CGGCTGA 3493
||||||||||||||||||||||||||||||||||| ||||||| ||| || | |||||||
Sbjct 3400 CTCAAAAACACTTTAAACGAACTCAGAAACAATCCGAAGCAAAAAGATCT-CTCGGCTGA 3458
Query 3494 AG-GCAACACAAAGGAGACTGATTCAGCTACCGATGAAGAGGAAGATGAGCTAATGGTTG 3552
| | ||||||||||||||||||||||||||| |||||||||||||||| |||||| |||
Sbjct 3459 CGAG-AACACAAAGGAGACTGATTCAGCTACCAATGAAGAGGAAGATGAACTAATGATTG 3517
Query 3553 AAGTTCCTGGTAGTGCCTACTCCATAGATCAGAAAATCAGTCATG---CCGCATAAAACT 3609
||||||||||||||||||||||||||||||||||||||| ||||| || |||| ||||
Sbjct 3518 AAGTTCCTGGTAGTGCCTACTCCATAGATCAGAAAATCAATCATGATGCCTCATAGAACT 3577
Query 3610 CATTTTTTGACTTGTC 3625
||||||||||||||||
Sbjct 3578 CATTTTTTGACTTGTC 3593
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 180500660914
Database: TAIR10_cdna_20110103_representative_gene_model_updated
Posted date: Sep 25, 2014 6:13 PM
Number of letters in database: 51,074,197
Number of sequences in database: 33,602
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5